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Aliases for ANO4 Gene

Aliases for ANO4 Gene

  • Anoctamin 4 2 3 5
  • Transmembrane Protein 16D 2 3 4
  • TMEM16D 3 4
  • Anoctamin-4 3

External Ids for ANO4 Gene

Previous HGNC Symbols for ANO4 Gene

  • TMEM16D

Previous GeneCards Identifiers for ANO4 Gene

  • GC12P099712
  • GC12P101188
  • GC12P098249
  • GC12P101111

Summaries for ANO4 Gene

GeneCards Summary for ANO4 Gene

ANO4 (Anoctamin 4) is a Protein Coding gene. Among its related pathways are Ion channel transport and Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds. GO annotations related to this gene include intracellular calcium activated chloride channel activity and phospholipid scramblase activity. An important paralog of this gene is ANO3.

UniProtKB/Swiss-Prot for ANO4 Gene

  • Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Does not exhibit calcium-activated chloride channel (CaCC) activity.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ANO4 Gene

Genomics for ANO4 Gene

Regulatory Elements for ANO4 Gene

Enhancers for ANO4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
- Elite enhancer/Elite enhancer-gene association

Enhancers around ANO4 on UCSC Golden Path with GeneCards custom track

Promoters for ANO4 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around ANO4 on UCSC Golden Path with GeneCards custom track

Genomic Location for ANO4 Gene

Chromosome:
12
Start:
100,717,463 bp from pter
End:
101,128,641 bp from pter
Size:
411,179 bases
Orientation:
Plus strand

Genomic View for ANO4 Gene

Genes around ANO4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ANO4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ANO4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ANO4 Gene

Proteins for ANO4 Gene

  • Protein details for ANO4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q32M45-ANO4_HUMAN
    Recommended name:
    Anoctamin-4
    Protein Accession:
    Q32M45
    Secondary Accessions:
    • Q8NAJ0
    • Q8NB39
    • Q8NB53

    Protein attributes for ANO4 Gene

    Size:
    955 amino acids
    Molecular mass:
    111462 Da
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • The term anoctamin was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.
    SequenceCaution:
    • Sequence=BAC03688.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Alternative splice isoforms for ANO4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ANO4 Gene

Post-translational modifications for ANO4 Gene

  • Glycosylation at Asn 83, Asn 105, Asn 257, and Asn 288
  • Modification sites at PhosphoSitePlus

Other Protein References for ANO4 Gene

No data available for DME Specific Peptides for ANO4 Gene

Domains & Families for ANO4 Gene

Gene Families for ANO4 Gene

Protein Domains for ANO4 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for ANO4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q32M45

UniProtKB/Swiss-Prot:

ANO4_HUMAN :
  • Belongs to the anoctamin family.
Family:
  • Belongs to the anoctamin family.
genes like me logo Genes that share domains with ANO4: view

Function for ANO4 Gene

Molecular function for ANO4 Gene

UniProtKB/Swiss-Prot Function:
Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Does not exhibit calcium-activated chloride channel (CaCC) activity.

Gene Ontology (GO) - Molecular Function for ANO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005229 intracellular calcium activated chloride channel activity TAS --
GO:0017128 phospholipid scramblase activity IEA --
GO:0046983 protein dimerization activity IEA --
genes like me logo Genes that share ontologies with ANO4: view

Phenotypes for ANO4 Gene

GenomeRNAi human phenotypes for ANO4:
genes like me logo Genes that share phenotypes with ANO4: view

Animal Model Products

CRISPR Products

miRNA for ANO4 Gene

miRTarBase miRNAs that target ANO4

Inhibitory RNA Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ANO4 Gene

Localization for ANO4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ANO4 Gene

Cell membrane; Multi-pass membrane protein. Note=Shows an intracellular localization. {ECO:0000250 UniProtKB:Q8C5H1}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ANO4 Gene COMPARTMENTS Subcellular localization image for ANO4 gene
Compartment Confidence
plasma membrane 5
peroxisome 2
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for ANO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular ISS --
GO:0005886 plasma membrane TAS --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with ANO4: view

Pathways & Interactions for ANO4 Gene

genes like me logo Genes that share pathways with ANO4: view

Pathways by source for ANO4 Gene

Interacting Proteins for ANO4 Gene

Selected Interacting proteins: Q32M45-ANO4_HUMAN for ANO4 Gene via IID

Symbol External ID(s) Details
FADD
NELL1

Gene Ontology (GO) - Biological Process for ANO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006821 chloride transport IDA 22946059
GO:0006869 lipid transport IEA --
GO:0034220 ion transmembrane transport TAS --
GO:0061589 calcium activated phosphatidylserine scrambling IEA --
GO:0061590 calcium activated phosphatidylcholine scrambling IEA --
genes like me logo Genes that share ontologies with ANO4: view

No data available for SIGNOR curated interactions for ANO4 Gene

Drugs & Compounds for ANO4 Gene

(1) Drugs for ANO4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
calcium Nutra 0

(1) Additional Compounds for ANO4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Chloride ion
  • Bertholite
  • Chloor
  • Chlor
  • Chlore
  • Chloride
16887-00-6
genes like me logo Genes that share compounds with ANO4: view

Transcripts for ANO4 Gene

Unigene Clusters for ANO4 Gene

Anoctamin 4:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ANO4 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^
SP1: - - -
SP2: -
SP3: -
SP4:

ExUns: 23 ^ 24 ^ 25 ^ 26 ^ 27 ^ 28 ^ 29
SP1:
SP2:
SP3:
SP4:

Relevant External Links for ANO4 Gene

GeneLoc Exon Structure for
ANO4
ECgene alternative splicing isoforms for
ANO4

Expression for ANO4 Gene

mRNA expression in normal human tissues for ANO4 Gene

mRNA differential expression in normal tissues according to GTEx for ANO4 Gene

This gene is overexpressed in Brain - Hippocampus (x4.1).

Protein differential expression in normal tissues from HIPED for ANO4 Gene

This gene is overexpressed in Cardia (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ANO4 Gene



Protein tissue co-expression partners for ANO4 Gene

NURSA nuclear receptor signaling pathways regulating expression of ANO4 Gene:

ANO4

SOURCE GeneReport for Unigene cluster for ANO4 Gene:

Hs.58785
genes like me logo Genes that share expression patterns with ANO4: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt for ANO4 Gene

Orthologs for ANO4 Gene

This gene was present in the common ancestor of animals.

Orthologs for ANO4 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia ANO4 34
  • 95.22 (n)
  • 98.59 (a)
ANO4 35
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ANO4 34
  • 94.97 (n)
  • 99.27 (a)
ANO4 35
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ano4 34
  • 92.08 (n)
  • 98.32 (a)
Ano4 16
Ano4 35
  • 98 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia ANO4 34
  • 99.76 (n)
  • 99.9 (a)
ANO4 35
  • 96 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ano4 34
  • 91.42 (n)
  • 99.07 (a)
oppossum
(Monodelphis domestica)
Mammalia ANO4 35
  • 77 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ANO4 35
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves ANO4 34
  • 86 (n)
  • 93.3 (a)
ANO4 35
  • 90 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ANO4 35
  • 90 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ano4 34
  • 79.06 (n)
  • 88.01 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.9960 34
fruit fly
(Drosophila melanogaster)
Insecta CG6938 35
  • 30 (a)
OneToMany
Species where no ortholog for ANO4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for ANO4 Gene

ENSEMBL:
Gene Tree for ANO4 (if available)
TreeFam:
Gene Tree for ANO4 (if available)

Paralogs for ANO4 Gene

Paralogs for ANO4 Gene

(7) SIMAP similar genes for ANO4 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with ANO4: view

Variants for ANO4 Gene

Sequence variations from dbSNP and Humsavar for ANO4 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs34162417 - 100,942,423(+) AGATG(C/G)AAAGT reference, missense, utr-variant-5-prime
rs1586105 -- 101,056,337(+) CTATA(C/T)ATTGG intron-variant
rs1588889 -- 101,063,822(-) tataa(A/C)caaat intron-variant
rs1606760 -- 100,771,594(-) ctgca(A/G)aaatc intron-variant
rs1606761 -- 100,771,591(-) CAGAA(A/G)TCTTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ANO4 Gene

Variant ID Type Subtype PubMed ID
dgv510e212 CNV loss 25503493
esv2586099 CNV deletion 19546169
esv2666281 CNV deletion 23128226
esv2673176 CNV deletion 23128226
esv27167 CNV loss 19812545
esv2763001 CNV loss 21179565
esv3630559 CNV loss 21293372
esv3630560 CNV gain 21293372
esv3630561 CNV loss 21293372
esv3630562 CNV loss 21293372
esv3630564 CNV loss 21293372
esv3630565 CNV loss 21293372
nsv1042478 CNV loss 25217958
nsv472341 CNV novel sequence insertion 20440878
nsv518877 CNV loss 19592680
nsv560005 CNV loss 21841781
nsv853 CNV deletion 18451855
nsv856 CNV insertion 18451855
nsv983358 CNV duplication 23825009

Variation tolerance for ANO4 Gene

Residual Variation Intolerance Score: 5.72% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.30; 76.56% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ANO4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ANO4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ANO4 Gene

Disorders for ANO4 Gene

Relevant External Links for ANO4

Genetic Association Database (GAD)
ANO4
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
ANO4

No disorders were found for ANO4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ANO4 Gene

Publications for ANO4 Gene

  1. FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. (PMID: 12739008) Katoh M. … Katoh M. (Int. J. Oncol. 2003) 2 3 4 65
  2. Structure and function of TMEM16 proteins (anoctamins). (PMID: 24692353) Pedemonte N. … Galietta L.J. (Physiol. Rev. 2014) 2 3 65
  3. Anoctamins are a family of Ca2+ activated Cl- channels. (PMID: 22946059) Tian Y. … Kunzelmann K. (J. Cell Sci. 2012) 3 4 65
  4. Identification and characterization of TMEM16E and TMEM16F genes in silico. (PMID: 15067359) Katoh M. … Katoh M. (Int. J. Oncol. 2004) 2 3 65
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 65

Products for ANO4 Gene

Sources for ANO4 Gene

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