Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



ANO1 Gene

protein-coding   GIFtS: 60
GCID: GC11P069929

Anoctamin 1, Calcium Activated Chloride Channel

(Previous names: oral cancer overexpressed 2, transmembrane protein 16A)
(Previous symbols: ORAOV2, TMEM16A)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Anoctamin 1, Calcium Activated Chloride Channel1 2     DOG12 3
TMEM16A1 2 3 5     TAOS22 3
ORAOV21 2 3     anoctamin-12
Oral Cancer Overexpressed 21 2     Ca2+-Activated Cl- Channel2
Transmembrane Protein 16A1 3     Transmembrane Protein 16A (Eight Membrane-Spanning Domains)2
Discovered On Gastrointestinal Stromal Tumors Protein 12 3     Oral Cancer Overexpressed Protein 23
Tumor-Amplified And Overexpressed Sequence 22 3     FLJ102615

External Ids:    HGNC: 216251   Entrez Gene: 551072   Ensembl: ENSG000001316207   OMIM: 6101085   UniProtKB: Q5XXA63   

Export aliases for ANO1 gene to outside databases

Previous GC identifers: GC11P069604 GC11P069925 GC11P066184 GC11P069927


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

GeneCards Summary for ANO1 Gene:
ANO1 (anoctamin 1, calcium activated chloride channel) is a protein-coding gene. Diseases associated with ANO1 include small intestinal sarcoma, and sarcocystosis. GO annotations related to this gene include intracellular calcium activated chloride channel activity. An important paralog of this gene is ANO9.

UniProtKB/Swiss-Prot: ANO1_HUMAN, Q5XXA6
Function: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and
smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which
generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal
and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage
development

summary for ANO1 Gene:
ANO1 functions as a calcium-activated chloride channel (CaCC). It is ubiquitously expressed in epithelia and
is thought to be important in epithelial fluid transport.

Gene Wiki entry for ANO1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000011.10  NT_167190.2  NC_018922.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ANO1 gene promoter:
         FOXF2   AP-1   Pax-2   FOXD1   Pax-2a   NF-E2 p45   C/EBPalpha   LyF-1   NF-E2   RSRFC4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): ANO1 promoter sequence
   Search Chromatin IP Primers for ANO1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ANO1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q13.3   Ensembl cytogenetic band:  11q13.3   HGNC cytogenetic band: 11q13.2

ANO1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ANO1 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P069929:  view genomic region     (about GC identifiers)

Start:
69,924,408 bp from pter      End:
70,035,651 bp from pter
Size:
111,244 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: ANO1_HUMAN, Q5XXA6 (See protein sequence)
Recommended Name: Anoctamin-1  
Size: 986 amino acids; 114078 Da
Subunit: Homodimer. Interacts with CFTR
Miscellaneous: The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT)
transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not
certain that all the members of this family are anion channels or have the 8-transmembrane topology
Secondary accessions: A8KAM3 Q8IYY8 Q8N7V3
Alternative splicing: 3 isoforms:  Q5XXA6-1   Q5XXA6-2   Q5XXA6-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ANO1: NX_Q5XXA6

Explore proteomics data for ANO1 at MOPED

Post-translational modifications: 

  • Glycosylation2 at Asn832
  • Modification sites at PhosphoSitePlus

  • See ANO1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_060513.5  
    ENSEMBL proteins: 
     ENSP00000347454   ENSP00000436392   ENSP00000319477   ENSP00000435797   ENSP00000433445  
     ENSP00000432843   ENSP00000435868   ENSP00000444689   ENSP00000381551  
    Reactome Protein details: Q5XXA6

    ANO1 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for ANO1
    GenScript Custom Purified and Recombinant Proteins Services for ANO1
    Novus Biologicals ANO1 Proteins
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for ANO1

    ANO1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of ANO1
    Browse R&D Systems for Antibodies
    OriGene Antibodies for ANO1
    OriGene Custom Antibody Services for ANO1
    Novus Biologicals ANO1 Antibodies
    Search for Antibodies for ANO1 at Abcam
    Cloud-Clone Corp. Antibodies for ANO1
    ThermoFisher Antibodies for ANO1
    LSBio Antibodies in human, mouse, rat for ANO1

    ANO1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ANO1
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ANO1
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ANO1
    Cloud-Clone Corp. CLIAs for ANO1


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    ANO: Ion channels / Chloride channels : Calcium activated : Anoctamins

    IUPHAR Guide to PHARMACOLOGY protein family classification: CaCC
    Calcium activated chloride channel

    1 InterPro protein domain:
     IPR007632 Anoctamin

    Graphical View of Domain Structure for InterPro Entry Q5XXA6

    ProtoNet protein and cluster: Q5XXA6

    UniProtKB/Swiss-Prot: ANO1_HUMAN, Q5XXA6
    Domain: The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region (By
    similarity)
    Similarity: Belongs to the anoctamin family


    ANO1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ANO1_HUMAN, Q5XXA6
    Function: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and
    smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which
    generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal
    and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage
    development
    Enzyme regulation: ATP and calmodulin are essential for its activation. Channel activity is inhibited by CFTR
    protein and by chloride inhibitors such as niflumic acid (NFA) and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic
    acid (DIDS)

         Gene Ontology (GO): 3 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005229intracellular calcium activated chloride channel activity ISS--
    GO:0005254chloride channel activity ----
    GO:0005515protein binding IPI--
         
    ANO1 for ontologies           About GeneDecksing


    Phenotypes:
         10 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Ano1):
     behavior/neurological  growth/size/body  homeostasis/metabolism  integument  mortality/aging 
     muscle  nervous system  no phenotypic analysis  respiratory system  skeleton 

    ANO1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Ano1tm1Bdh for ANO1

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ANO1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ANO1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ANO1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ANO1

    miRNA
    Products:
        
    miRTarBase miRNAs that target ANO1:
    hsa-mir-124-3p (MIRT022406), hsa-mir-335-5p (MIRT018614)

    Block miRNA regulation of human, mouse, rat ANO1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ANO1 (see all 36):
    hsa-miR-361-5p hsa-miR-300 hsa-miR-374c hsa-miR-9 hsa-miR-1183 hsa-miR-2113 hsa-miR-181b hsa-miR-1290
    SwitchGear 3'UTR luciferase reporter plasmidANO1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ANO1
    Predesigned siRNA for gene silencing in human, mouse, rat ANO1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ANO1

    Clone
    Products:
         
    OriGene clones in human, mouse for ANO1 (see all 13)
    OriGene ORF clones in mouse, rat for ANO1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ANO1 (NM_018043)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ANO1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ANO1

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for ANO1
    Browse ESI BIO Cell Lines and PureStem Progenitors for ANO1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ANO1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    ANO1_HUMAN, Q5XXA6: Cell membrane; Multi-pass membrane protein. Cytoplasm. Note=Cytoplasmic localization seen in
    neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    cytoskeleton2
    peroxisome2
    endoplasmic reticulum1
    nucleus1

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--
    GO:0005886plasma membrane TAS--
    GO:0016021integral component of membrane ----
    GO:0034707chloride channel complex IEA--
    GO:0070062extracellular vesicular exosome IDA19199708

    ANO1 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for ANO1 About    
    See pathways by source

    SuperPathContained pathways About
    1Ion channel transport
    Ion channel transport0.58
    Stimuli-sensing channels0.58
    2Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds
    Transmembrane transport of small molecules0.47

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for ANO1
        Stimuli-sensing channels



    ANO1 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ANO1
    Interactions:

        Search GeneGlobe Interaction Network for ANO1

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    2 Interacting proteins for ANO1 (Q5XXA63 ENSP000003474544) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CDC42P609533I2D: score=1 
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006821chloride transport ISS--
    GO:0007275multicellular organismal development IEA--
    GO:0034220ion transmembrane transport TAS--
    GO:0042391regulation of membrane potential IEA--
    GO:0055085transmembrane transport TAS--

    ANO1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Compounds for ANO1 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    DCPIB Selective blocker of VSAC/ICl, swell. Inhibits glucose-stimulated insulin release [82749-70-0]
    Chromanol 293BIKs blocker. Also blocks ICFTR[163163-23-3]
    NPPBInhibits calcium-sensitive chloride currents[107254-86-4]
    LonidamineMitochondrial hexokinase inhibitor[50264-69-2]
    CFTRinh 172 Voltage-independent, selective CFTR chloride channel blocker [307510-92-5]

    2 HMDB Compounds for ANO1    About this table
    CompoundSynonyms CAS #PubMed Ids
    CalciumCa (see all 2)7440-70-2--
    ChlorineCl2 (see all 13)16887-00-6--

    Selected IUPHAR Ligands for ANO1 (CaCC) (see all 12)    About this table
    LigandTypeActionAffinityPubmed IDs
    DCDPC
    Channel blockerNone--
    fluoxetine
    Channel blockerNone--
    tannic acid
    Channel blockerNone--
    SITS
    Channel blockerNone--
    9-A-C
    Channel blockerNone--
    niflumic acid
    Channel blockerNone--
    flufenamic acid
    Channel blockerNone--
    mibefradil
    Channel blockerNone--
    Ins(3,4,5,6)P4
    Channel blockerNone--
    Ca2+
    ActivatorNone--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for ANO1 gene: 
    NM_018043.5  

    Unigene Cluster for ANO1:

    Anoctamin 1, calcium activated chloride channel
    Hs.503074  [show with all ESTs]
    Unigene Representative Sequence: AY728143
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000355303(uc001opj.3 uc001opl.2 uc010rqk.2) ENST00000531604
    ENST00000316296(uc001opk.1) ENST00000530676 ENST00000530480 ENST00000529913
    ENST00000531349 ENST00000531300 ENST00000529636 ENST00000525494(uc010rql.1)
    ENST00000538023 ENST00000398543
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat ANO1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ANO1 (see all 36):
    hsa-miR-361-5p hsa-miR-300 hsa-miR-374c hsa-miR-9 hsa-miR-1183 hsa-miR-2113 hsa-miR-181b hsa-miR-1290
    SwitchGear 3'UTR luciferase reporter plasmidANO1 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for ANO1
    Predesigned siRNA for gene silencing in human, mouse, rat ANO1
    Clone
    Products:
         
    OriGene clones in human, mouse for ANO1 (see all 13)
    OriGene ORF clones in mouse, rat for ANO1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ANO1 (NM_018043)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ANO1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ANO1
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for ANO1
    OriGene qSTAR qPCR primer pairs in human, mouse for ANO1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ANO1
      QuantiTect SYBR Green Assays in human, mouse, rat ANO1
      QuantiFast Probe-based Assays in human, mouse, rat ANO1

    Additional mRNA sequence: 

    AK001123.1 AK293088.1 AK303971.1 AL833582.1 AY728143.1 BC027590.2 HQ418153.1 NR_030691.1 

    9 DOTS entries:

    DT.101980576  DT.410711  DT.100019792  DT.120698490  DT.92032108  DT.92431354  DT.99930906  DT.120698476 
    DT.91664586 

    Selected AceView cDNA sequences (see all 134):

    BF446942 AI905727 AA553708 BQ082130 AI983144 AI055977 BM842120 AI168140 
    BM840190 CA449823 BQ446478 BF438336 BM841770 BE184802 AL833582 BG055062 
    AI697426 NM_018043 AI521498 BF803248 BF593296 AI979079 AI698683 BC033036 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ANO1 (see all 6)    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19a · 19b ^ 20a · 20b ^ 21 ^
    SP1:              -     -     -                                                                                                           -     -               
    SP2:                                                                                                        -                             -     -               
    SP3:                                                                                                        -                             -     -               
    SP4:                          -                                                                             -                                                   
    SP5:              -     -     -           -                                                                                                                     

    ExUns: 22 ^ 23a · 23b ^ 24 ^ 25 ^ 26a · 26b ^ 27
    SP1:                                                
    SP2:                                      -         
    SP3:                                                
    SP4:                                                
    SP5:                                                


    ECgene alternative splicing isoforms for ANO1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    ANO1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CAATCTGATG
    ANO1 Expression
    About this image


    ANO1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 18) fully expand
     
     Kidney (Urinary System)    fully expand to see all 13 entries
             Loop of Henle Cells Loop of Henle
             Proximal Tubule
     
     Neural Tube (Nervous System)    fully expand to see all 4 entries
             Telencephalon
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Thalamus
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Pre-Granulosa Cells Primordial Follicle
             Oviduct
     
     Intermediate Mesoderm (Gastrulation Derivatives)    fully expand to see all 2 entries
             Mesonephric cells Mesonephros
             Mesonephros
    ANO1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ANO1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.503074

    UniProtKB/Swiss-Prot: ANO1_HUMAN, Q5XXA6
    Tissue specificity: Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles

        Custom PCR Arrays for ANO1
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for ANO1
    OriGene qSTAR qPCR primer pairs in human, mouse for ANO1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ANO1
    QuantiTect SYBR Green Assays in human, mouse, rat ANO1
    QuantiFast Probe-based Assays in human, mouse, rat ANO1
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ANO1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for ANO1 gene from Selected species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ano11 , 5 anoctamin 1, calcium activated chloride channel1, 5 85.44(n)1
    92.28(a)1
      7 (88.85 cM)5
    1017721  NM_178642.51  NP_848757.41 
     1445885495 
    chicken
    (Gallus gallus)
    Aves ANO11 anoctamin 1, calcium activated chloride channel 77.08(n)
    87.07(a)
      423144  XM_004941529.1  XP_004941586.1 
    lizard
    (Anolis carolinensis)
    Reptilia ANO16
    anoctamin 1, calcium activated chloride channel
    87(a)
    1 ↔ 1
    GL343283.1(1275-57379)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia ano11 anoctamin 1, calcium activated chloride channel 73.5(n)
    80.88(a)
      100170550  NM_001130327.1  NP_001123799.1 
    zebrafish
    (Danio rerio)
    Actinopterygii CD759475.12   -- 76.69(n)    CD759475.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG167181 CG16718 53.57(n)
    46.04(a)
      42340  NM_001275807.1  NP_001262736.1 


    ENSEMBL Gene Tree for ANO1 (if available)
    TreeFam Gene Tree for ANO1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for ANO1 gene
    ANO92  ANO62  ANO22  ANO52  ANO102  ANO32  ANO42  ANO82  
    ANO72  
    8 SIMAP similar genes for ANO1 using alignment to 7 protein entries:     ANO1_HUMAN (see all proteins):
    ANO2    ANO3    ANO4    ANO5    ANO7    ANO6
    DKFZp434P102    ANO9

    ANO1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for ANO1 (see all 2797)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs576478581,2
    C--69868690(+) TTTTT-/TTGAGAC 2 -- int11Minor allele frequency- TT:0.00NA 2
    rs1833474221,2
    --69923550(+) ATGGCC/TCCTGG 1 -- us2k10--------
    rs1482993791,2
    --69923689(+) CCCCCA/GCTCCG 1 -- us2k10--------
    rs108979641,2
    C,F,A--69923717(+) ACAGCC/TTTGCC 1 -- us2k16Minor allele frequency- T:0.16NA WA EA 364
    rs1417656651,2
    C--69923764(+) CCTCAC/GAGGAT 1 -- us2k10--------
    rs1181560201,2
    F--69923833(+) TTCCAA/GCCTTG 1 -- us2k11Minor allele frequency- G:0.04EA 120
    rs1150289921,2
    C,F--69923874(+) CTCACG/ACCCTA 1 -- us2k11Minor allele frequency- A:0.03WA 118
    rs1472163181,2
    --69924048(+) CCCCGA/GAGGAG 1 -- us2k10--------
    rs1879007211,2
    --69924071(+) TGACAA/GCAGGA 1 -- us2k10--------
    rs1180330381,2
    F--69924094(+) GGAGGG/AAAAGG 1 -- us2k11Minor allele frequency- A:0.02NA 120

    HapMap Linkage Disequilibrium report for ANO1 (69924408 - 70035651 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for ANO1 (see all 23):    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv222e199CNV Deletion23128226
    esv2744709CNV Deletion23290073
    esv2744704CNV Deletion23290073
    esv1360750CNV Deletion17803354
    esv2744705CNV Deletion23290073
    esv2744707CNV Deletion23290073
    esv2744710CNV Deletion23290073
    esv2744706CNV Deletion23290073
    nsv820744CNV Deletion20802225
    esv1620418CNV Deletion17803354

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ANO1
    DNA2.0 Custom Variant and Variant Library Synthesis for ANO1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 610108    OMIM disorders: --

    20 diseases for ANO1:    
    About MalaCards
    small intestinal sarcoma    sarcocystosis    toxic pneumonitis    gastrointestinal stromal tumor
    oral cancer    chondroblastoma    gastroparesis    constipation
    leiomyosarcoma    cerebellar ataxia    sinusitis    labyrinthitis
    ataxia    cystic fibrosis    sarcoma    hypertension
    squamous cell carcinoma    pancreatitis    cerebritis    breast cancer

    2 diseases from the University of Copenhagen DISEASES database for ANO1:
    Gastrointestinal stromal tumor     Small intestinal sarcoma

    ANO1 for disorders           About GeneDecksing

    1 Novoseek inferred disease relationship for ANO1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 0 3 12739008 (1), 15375614 (1)

    Genetic Association Database (GAD): ANO1
    Human Genome Epidemiology (HuGE) Navigator: ANO1 (4 documents)

    Export disorders for ANO1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for ANO1 gene, integrated from 10 sources (see all 82):
    (articles sorted by number of sources associating them with ANO1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. (PubMed id 12739008)1, 2, 9 Katoh M. and Katoh M. (Int. J. Oncol. 2003)
    2. Identification and characterization of TMEM16E and TMEM16F genes in silico. (PubMed id 15067359)1, 3, 9 Katoh M. and Katoh M. (Int. J. Oncol. 2004)
    3. CFTR and TMEM16A are separate but functionally related Cl-channels. (PubMed id 22178883)1, 2 Ousingsawat J.... Kunzelmann K. (Cell. Physiol. Biochem. 2011)
    4. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    5. Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. (PubMed id 19773279)1, 4 Hosgood H.D....Lan Q. (Occup Environ Med 2009)
    6. TMEM16A confers receptor-activated calcium-dependent chloride conductance. (PubMed id 18724360)1, 3 Yang Y.D....Oh U. (Nature 2008)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    8. Non-canonical translation start sites in the TMEM16A chloride channel. (PubMed id 23994600)1 Sondo E....Galietta L.J. (Biochim. Biophys. Acta 2014)
    9. Functional expression of the TMEM16 family of calcium-activated chloride channels in airway smooth muscle. (PubMed id 23997176)1 Gallos G....Emala C.W. (Am. J. Physiol. Lung Cell Mol. Physiol. 2013)
    10. TMEM16A alternative splicing coordination in breast cancer. (PubMed id 23866066)1 Ubby I....Pagani F. (Mol. Cancer 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 55107 HGNC: 21625 AceView: TMEM16A Ensembl:ENSG00000131620 euGenes: HUgn55107
    ECgene: ANO1 H-InvDB: ANO1

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for ANO1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for ANO1 gene:
    Search GeneIP for patents involving ANO1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Kits and Assays available from EMD Millipore
     Browse Small Molecules at EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of ANO1
     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for ANO1   OriGene RNAi products in human, mouse, rat for ANO1  
     OriGene qPCR primer pairs and template standards for ANO1   Browse OriGene Protein Over-expression Lysates  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for ANO1  
     OriGene qSTAR qPCR primer pairs in human, mouse for ANO1   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for ANO1   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for ANO1   OriGene Custom Protein Services for ANO1  

     
     
     Block miRNA regulation of human, mouse, rat ANO1 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing ANO1
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ANO1 Predesigned siRNA for gene silencing in human, mouse, rat ANO1
     QuantiFast Probe-based Assays in human, mouse, rat ANO1 QuantiTect SYBR Green Assays in human, mouse, rat ANO1
     Custom PCR Arrays for ANO1 Search Chromatin IP Primers for ANO1
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ANO1  Search GeneGlobe Interaction Network for ANO1
     Regulatory tfbs in ANO1 promoter
     GenScript Custom Purified and Recombinant Proteins Services for ANO1 GenScript cDNA clones with any tag delivered in your preferred vector for ANO1
     GenScript Custom Assay Services for ANO1 GenScript Custom overexpressing Cell Line Services for ANO1
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Tocris compounds for ANO1
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     ANO1 antibodies
     ANO1 proteins
     Search for Antibodies for ANO1 at Abcam
     See all of Abcam's Antibodies, Kits and Proteins for ANO1
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for ANO1
     Antibodies for ANO1
     ELISAs for ANO1
     CLIAs for ANO1



     Browse ESI BIO Cell Lines and PureStem Progenitors for ANO1
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ANO1
     SwitchGear 3'UTR luciferase reporter plasmids for ANO1
     SwitchGear Promoter luciferase reporter plasmids for ANO1
     ThermoFisher Antibodies for ANO1
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ANO1
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ANO1
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ANO1
     LSBio Antibodies in human, mouse, rat for ANO1
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      ANO1 gene at Home site.
    Version: 3.12.162 20 Aug 2014
    hostname: 356980-web2.xennexinc.com index build: 126 solr: 1.4