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ANKRD65 Gene

protein-coding   GIFtS: 32
GCID: GC01M001358

Ankyrin Repeat Domain 65

  Search for ANKRD65
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ankyrin Repeat Domain 651 2
Ankyrin Repeat Domain-Containing Protein 652

External Ids:    HGNC: 429501   Entrez Gene: 4418692   Ensembl: ENSG000002350987   UniProtKB: E5RJM63   

Export aliases for ANKRD65 gene to outside databases

Previous GC identifer: GC01M001355


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ANKRD65 Gene:
ANKRD65 (ankyrin repeat domain 65) is a protein-coding gene. An important paralog of this gene is TNKS2.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000001.10  NT_032977.10  NC_018912.2  
Regulatory elements:
   Search for regulatory transcription factor binding sites for ANKRD65
         Other transcription factors

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Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ANKRD65


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p36.33   Ensembl cytogenetic band:  1p36.33   HGNC cytogenetic band: 1p36.33

ANKRD65 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ANKRD65 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M001358:  view genomic region     (about GC identifiers)

Start:
1,353,800 bp from pter      End:
1,357,149 bp from pter
Size:
3,350 bases      Orientation:
minus strand
ORGUL member locations:
Legend (see complete legend)

ORGUL Member Locations for ANKRD65

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ANR65_HUMAN, E5RJM6 (See protein sequence)
Recommended Name: Ankyrin repeat domain-containing protein 65  
Size: 399 amino acids; 41497 Da
Secondary accessions: J3KR93
Alternative splicing: 3 isoforms:  E5RJM6-1   E5RJM6-2   E5RJM6-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ANKRD65: NX_E5RJM6

Explore proteomics data for ANKRD65 at MOPED


See ANKRD65 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (3 alternative transcripts): 
NP_001138682.1  NP_001230464.1  NP_001230465.1  

ENSEMBL proteins: 
 ENSP00000429035   ENSP00000428419   ENSP00000428201   ENSP00000427903   ENSP00000445688  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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HGNC Gene Families:
ANKRD: Ankyrin repeat domain containing

2 InterPro protein domains:
 IPR002110 Ankyrin_rpt
 IPR020683 Ankyrin_rpt-contain_dom

Graphical View of Domain Structure for InterPro Entry E5RJM6

ProtoNet protein and cluster: E5RJM6

UniProtKB/Swiss-Prot: ANR65_HUMAN, E5RJM6
Similarity: Contains 10 ANK repeats


Find genes that share domains with ANKRD65           About GenesLikeMe


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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     Gene Ontology (GO): 1 molecular function term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005515protein binding ----
     
Find genes that share ontologies with ANKRD65           About GenesLikeMe


Phenotypes:
     1 GenomeRNAi human phenotype for ANKRD65:
 Metaphase cells 

Animal Models:
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ANKRD65
Interactions:

    Search GeneGlobe Interaction Network for ANKRD65

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for ANKRD65 (ANR65)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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REFSEQ mRNAs for ANKRD65 gene (3 alternative transcripts): 
NM_001145210.2  NM_001243535.1  NM_001243536.1  

Unigene Cluster for ANKRD65:

Ankyrin repeat domain 65
Hs.381222  [show with all ESTs]
Unigene Representative Sequence: NM_001145210
6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000520296(uc001afp.3) ENST00000427211(uc001afq.3) ENST00000454272
ENST00000442470 ENST00000436362 ENST00000537107(uc010nyo.2)
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Additional mRNA sequence: 

BC018095.1 BC063445.1 BC073150.1 BC094869.1 M32220.1 

6 DOTS entries:

DT.100761671  DT.91690961  DT.95371719  DT.70103963  DT.100761676  DT.95242476 

Selected AceView cDNA sequences (see all 74):

BC063445 BM142089 BF515859 BU731480 BE218962 BF724652 BM563298 BU170270 
AI922941 AI094806 AI184936 CK819891 AI522076 BE503132 BI755193 CB321756 
AI190276 BC073150 BM353987 BE263901 AI539403 BX386219 BF341239 BI770529 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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ANKRD65 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: TGAAATCTTC
ANKRD65 Expression
About this image

ANKRD65 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

ANKRD65 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.381222
    Custom PCR Arrays for ANKRD65
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In Situ
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(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for ANKRD65 gene from Selected species (see all 9)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Ankrd651 , 5 RIKEN cDNA E230028L10 gene5
ankyrin repeat domain 651
69.96(n)1
67.51(a)1
  4 (87.39 cM)5
2428051  XM_006539421.11  XP_006539484.11 
 1557911725 
lizard
(Anolis carolinensis)
Reptilia ANKRD656
ankyrin repeat domain 65
41(a)
1 ↔ 1
GL343701.1(77665-85256)
tropical clawed frog
(Xenopus tropicalis)
Amphibia ankrd651 ankyrin repeat domain 65 49(n)
43.46(a)
  101731183  XM_004916184.1  XP_004916241.1 
zebrafish
(Danio rerio)
Actinopterygii ANKRD656
ankyrin repeat domain 65
34(a)
1 ↔ 1
18(39836152-39850284) ENSDARG00000068274
worm
(Caenorhabditis elegans)
Secernentea trp-46
Protein TRP-4 (trp-4) mRNA, complete cds
8(a)
1 ↔ 1
I(13899085-13913966) WBGene00006616


ENSEMBL Gene Tree for ANKRD65 (if available)
TreeFam Gene Tree for ANKRD65 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for ANKRD65 gene
TNKS22  ANK32  ANK12  ANKRD522  TNKS2  ANKFY12  ANKRD502  INVS2  
ANK22  ANKRD282  
Selected SIMAP similar genes for ANKRD65 using alignment to 2 protein entries:     ANR65_HUMAN (see all proteins) (see all similar genes):
ANKRD54    RAI14    ANKRD12    ASB8    FPGT-TNNI3K    GABPB1
PPP1R12A    DKFZp761E1322    MTPN    ANK2    PSMD10    ANKRD6
ANKDD1A    ANKRD44    TNKS    CASKIN2    IkBa    ANKRD42

Find genes that share paralogs with ANKRD65           About GenesLikeMe



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for ANKRD65 (see all 99)    About this table                                 

Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 1 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs114884621,2
C,F,H--1353380(+) TCACAT/CGATCT 3 -- int115Minor allele frequency- C:0.23NS EA NA WA 1700
rs1879091411,2
--1353400(+) CAAAAA/CCAGAA 3 -- int10--------
rs1927947001,2
--1353401(+) AAAACA/CAGAAC 3 -- int10--------
rs1152220721,2
F--1353430(+) ACGCCG/ATGGGC 3 -- int11Minor allele frequency- A:0.01NA 120
rs1843335371,2
--1353525(+) GCCAGG/TTCCTT 3 -- int10--------
rs49704471,2
C,F--1353561(+) CCACCG/ATGGGG 3 -- int15Minor allele frequency- A:0.50NA WA 10
rs1114363711,2
C,F--1353591(+) GGCGAG/TGTGTT 3 -- int12Minor allele frequency- T:0.04CSA WA 120
rs789911091,2
F--1353601(+) TCCACT/CGAGGC 3 -- int11Minor allele frequency- C:0.00NA 2
rs49704481,2
C--1353603(+) CACCGG/AGGCAA 3 -- int13Minor allele frequency- A:0.17WA NA 6
rs1819488321,2
--1353630(+) GCTCTC/TTAGCT 3 -- int10--------

HapMap Linkage Disequilibrium report for ANKRD65 (1353800 - 1357149 bp)

Structural Variations
      Database of Genomic Variants (DGV) variations for ANKRD65: --
Site Specific Mutation Identification with PCR Assays
Search QIAGEN SeqTarget long-range PCR primers for resequencing ANKRD65
DNA2.0 Custom Variant and Variant Library Synthesis for ANKRD65

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for ANKRD65 gene integrated from 10 sources:
(articles sorted by number of sources associating them with ANKRD65)
    Utopia: connect your pdf to the dynamic
world of online information

  1. The HECT type ubiquitin ligase NEDL2 is degraded by anaphase-promoting complex/cyclosome (APC/C)-Cdh1, and its tight regulation maintains the metaphase to anaphase transition. (PubMed id 24163370)1 Lu L....Zhang L. (J. Biol. Chem. 2013)
  2. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)2 Gregory S.G.... Bentley D.R. (Nature 2006)
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  4. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  5. The sequence of the human genome. (PubMed id 11181995)1 Venter J.C.... Zhu X. (Science 2001)
  6. Transcription of human endogenous retroviral long terminal repeat (LTR) sequence in a lung cancer cell line. (PubMed id 2302192)1 Tomita N....Matsubara K. (Biochem. Biophys. Res. Commun. 1990)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 441869 HGNC: 42950 AceView: LOC441869 Ensembl:ENSG00000235098 euGenes: HUgn441869
ECgene: ANKRD65 H-InvDB: ANKRD65

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for ANKRD65 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for ANKRD65 gene:
Search GeneIP for patents involving ANKRD65

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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 See all of Abcam's Antibodies, Kits and Proteins for ANKRD65
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 Browse ProSpec Recombinant Proteins
 Browse Proteins at Cloud-Clone Corp.
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 Browse CLIAs at Cloud-Clone Corp.



 Browse ESI BIO Cell Lines and PureStem Progenitors for ANKRD65
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 Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ANKRD65
 Browse SwitchGear 3'UTR luciferase reporter plasmids for ANKRD65
 Browse SwitchGear Promoter luciferase reporter plasmids for ANKRD65
 Search ThermoFisher Antibodies for ANKRD65
 Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat ANKRD65
 inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ANKRD65
 inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ANKRD65
 LSBio Antibodies in human, mouse, rat for ANKRD65
Customized transgenic rodents for:
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 Browse compounds at ApexBio
 Search Addgene for plasmids for ANKRD65
  Search eBioscience for proteins for ANKRD65
  Search eBioscience for elisas for ANKRD65
  eBioscience FlowRNA Probe Sets
       
GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

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