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ANAPC7 Gene

protein-coding   GIFtS: 51

GC12M109295
anaphase promoting complex subunit 7
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
APC7 1, 2, 3
Descriptions
Cyclosome subunit 7 2, 3
anaphase promoting complex subunit 7 2
anaphase-promoting complex subunit 7 2
External Ids
HGNC: 173801
Entrez Gene: 514342
UniProtKB: Q9UJX33
Ensembl: ENSG000001965107
Search outside databases for aliases for ANAPC7 gene

Previous GC identifers: GC12M9C0183 GC12P9H0002 GC12U900269 GC12M109274

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for ANAPC7:
This gene encodes a tetratricopeptide repeat containing component of the anaphase promoting
complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by
targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated
degradation through ubiquitination. The encoded protein is required for proper protein
ubiquitination function of APC/C and for the interaction of APC/C with certain transcription
coactivators. Multiple transcript variants encoding different isoforms have been found for this
gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: APC7_HUMAN, Q9UJX3
Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated
ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle

Gene Wiki entry for ANAPC7

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ANAPC7 gene  

Entrez Gene cytogenetic band: 12q24.11   Ensembl cytogenetic band:  12q24.11   HGNC cytogenetic band: 12q13.12

ANAPC7 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M109295:     (about GC identifiers)

Start:
109,295,667 bp from pter
End:
109,325,868 bp from pter
Size:
30,202 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000012.10  NT_009775.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: APC7_HUMAN, Q9UJX3 (See protein sequence)
Recommended Name: Anaphase-promoting complex subunit 7  
Size: 565 amino acids; 63133 Da
Subunit: The APC/C is composed of at least 11 subunits
Sequence caution: Sequence=AAH17316.2; Type=Erroneous translation; Note=Wrong choice of frame;
PDB structures from and Proteopedia :
3FFL (3D)    
Secondary accessions: Q96AC4 Q96GF4 Q9BU24 Q9NT16

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001131136.1  NP_057322.2  


    ENSEMBL proteins: 
    ENSP00000346363 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Apc7)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005654 nucleoplasm EXP10548110 11340163 15029244
    GO:0005829 cytosol EXP10793135 11285280 11535616 11742988 12070128
    About this table

    Antibodies for ANAPC7: 
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    Novus Biologicals Antibody for ANAPC7

    Assays for ANAPC7: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001440 TPR-1
     IPR011990 TPR-like_helical
     IPR013026 TPR_region
     IPR019734 TPR_repeat


       GeneDecks  ANAPC7 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UJX3

    ProtoNet protein and cluster: Q9UJX3

    UniProtKB/Swiss-Prot: APC7_HUMAN, Q9UJX3
    Similarity: Belongs to the APC7 family
    Similarity: Contains 7 TPR repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ANAPC7)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_016238

                  Applied Biosystems Silencer® siRNAs for ANAPC7

                  Sigma-Aldrich siRNA and siRNA Panels for ANAPC7  
                         Sigma-Aldrich shRNA for ANAPC7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001137664
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001137664
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001137664 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_016238

    UniProtKB/Swiss-Prot: APC7_HUMAN, Q9UJX3
    Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated
    ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005488 binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    4 Invitrogen iPath™ Online BioAtlas - Pathways for ANAPC7 (Maps provided by GeneGo):
     PKA signaling
     Anaphase promoting complex (APC) Regulation
     TGF-beta receptor signaling
     G-Protein alpha-s signaling cascades

       GeneDecks  ANAPC7 for the pathways selected above  
    About GeneDecksing

    5/9 Millipore Pathways for ANAPC7 (see all 9 )
     Cell cycle Role of APC in cell cycle regulation
     Meiosis
     Cell cycle Regulation of G1/S transition (part 1)
     Development TGF-beta receptor signaling
     Signal transduction PKA signaling

       GeneDecks  ANAPC7 for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  ANAPC7  (Your Favorite Gene powered by Ingenuity) 
     Mitotic Roles of Polo-Like Kinase

       GeneDecks  ANAPC7 for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for ANAPC7):
     hsa04110 Cell cycle
     hsa04120 Ubiquitin mediated proteolysis

       GeneDecks  ANAPC7 for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: APC7_HUMAN, Q9UJX3
    Pathway: Protein modification; protein ubiquitination

     Gene Network CentralTM Interacting Genes and Proteins Network for  ANAPC7 


    5/77 Interacting proteins for ANAPC7 (ENSP000003463633) via UniProtKB, MINT, and/or STRING (see all 77 )

    InteractantInteraction Details
    GeneCardExternal ID(s)
    CDC27ENSP000000665443STRING (score=.996)
    ANAPC1ENSP000003391093STRING (score=.988)
    ANAPC4ENSP000003187753STRING (score=.987)
    CDC20ENSP000003084503STRING (score=.986)
    ANAPC5ENSP000002618193STRING (score=.969)
    About this table

    5/7 Gene Ontology (GO) biological process terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007049 cell cycle IEA--
    GO:0007067 mitosis IEA--
    GO:0019941 modification-dependent protein catabolic process IEA--
    GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process EXP11285280
    GO:0051301 cell division IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ANAPC7

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ANAPC7)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_016238

                  Sigma-Aldrich siRNA and siRNA Panels for ANAPC7  
                         Sigma-Aldrich shRNA for ANAPC7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001137664  NM_016238  

    REFSEQ mRNAs for ANAPC7 gene (2 alternative transcripts): 

    NM_001137664.1   NM_016238.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001137664  NM_016238  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001137664
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001137664
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001137664 

    Additional cDNA sequence: 

    AF004561.1 AF006086.1 AF191340.1 AK021809.1 AK297309.1 AK311789.1 AL137586.1 AL157481.1 
    AL357196.1 AY007104.1 BC002941.2 BC009498.2 BC017316.2 BC040130.1 BC067747.1 BC078162.1 
    BC098264.1 BC098295.1 BC098362.1 BC099732.1 BC111798.1 BC141848.1 BC148236.2 CR407667.1 
    CR595174.1 CR606213.1 CR611961.1 CR616701.1 DQ328220.1 

    24/47 DOTS entries (see all 47 ):

    DT.95290197  DT.100666390  DT.95162379  DT.97862620  DT.100666385  DT.444484  DT.99950552  DT.100782748 
    DT.102845358  DT.95162376  DT.312557  DT.121216052  DT.100666386  DT.121216159  DT.120690346  DT.121216120 
    DT.92453362  DT.121216080  DT.121216306  DT.100666384  DT.92453363  DT.40108271  DT.75112546  DT.86859893 

    24/983 AceView cDNA sequences (see all 983 ):

    BU195572 AI298479 CB069455 BU153627 AL137586 AW161094 BQ003692 AI373621 
    BM792288 AA159032 CA309839 BE910721 CR595174 CB159862 BI715388 BQ922005 
    AI621325 BG387564 AA629219 BM841738 BM550338 BM451842 BG825894 BG825422 

    highest scoring ESTs for ANAPC7:

    AA131793 AA334730 AF191340 AI358798 AI690444 AI910731 AK021809 AU143374 AW135464 AW471105 

    Unigene Cluster for ANAPC7:

    Anaphase promoting complex subunit 7
    Hs.524741  [show with all ESTs]
    Unigene Representative Sequence: NM_016238


    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for ANAPC7 (see all 11 )

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b · 9c ^ 10 ^ 11a · 11b ^ 12a · 12b ^
    SP1:                          -     -                                               -                 -                             -     -                 -   
    SP2:                          -     -                                               -                 -                             -     -                     
    SP3:                                                                                -                                                                           
    SP4:                          -     -     -     -                                   -                                                                           
    SP5:                          -     -                       -                                                                                                   

    ExUns: 13a · 13b · 13c
    SP1:                  
    SP2:                  
    SP3:                  
    SP4:                  
    SP5:                  

    About this scheme

    ECgene alternative splicing isoforms for ANAPC7

    1 Ensembl transcript including schematic representation:
    ENST00000354390  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ANAPC7 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ANAPC7

    1 / 2 / 3

    7 probe-sets matching ANAPC7 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    45808_at2, 3 U95-B 1 1.00 1.00 0.72 1.39 AA131793 0.80 0.88 0.84 1

    50144_at2, 3 U95-C 1 1.00 1.00 0.30 1.19 W72350 0.80 0.88 0.84 1

    63079_at2, 3 U95-C 1 1.00 1.00 0.79 0.44 T67474 0.20 1.00 0.72 1

    225554_s_at2, 3 U133-B 1 1.00 1.00 -- -- AA131793 0.80 0.88 0.84 1

    225521_at2, 3 U133-B 1 1.00 1.00 -- -- AL137586 0.60 0.83 0.73 1

    225554_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    225521_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.524741
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ANAPC7 gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ANAPC71   -- anaphase promoting complex subunit 7 96.05(n)
    99.29(a)
    486264  XM_543390.2  XP_543390.2 
    chimpanzee
    (Pan troglodytes)
    ANAPC71   -- anaphase promoting complex subunit 7 99.64(n)
    99.45(a)
    467128  XM_522528.2  XP_522528.2 
    cow
    (Bos taurus)
    ANAPC71   -- anaphase promoting complex subunit 7 95.4(n)
    99.12(a)
    535072  XM_615057.3  XP_615057.3 
    rat
    (Rattus norvegicus)
    Anapc71   -- anaphase promoting complex subunit 7 88.24(n)
    98(a)
    304490  XM_222171.4  XP_222171.4 
    mouse
    (Mus musculus)
    Anapc71, 5 55
    anaphase promoting complex subunit 71, 5 88.55(n)1
    97.52(a)1
    563171  NM_019805.31  NP_062779.21 
     AF0766075  AI4482945  (see all 20)
    About this table        Species with no ortholog for ANAPC7

    ENSEMBL Gene Tree for ANAPC7
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/144 NCBI SNPs in ANAPC7 are shown (see all 144 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 17)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs285552141,2
    C109313419(+) TAGATA/CCTCAC 1 -- int10--------
    --
    rs66067461,2
    C109301077(-) agagcA/Gagact 1 -- int10--------
    --
    rs286090961,2
    C109300305(+) GCGACA/GGAGTT 1 -- int10--------
    --
    rs41318511,2
    C109296728(-) ACTCTA/GAAAAT 1 -- int10--------
    --
    rs619425891,2
    --109318603(+) ATACAA/CCAAAA 1 V/L mis10--------
    --
    rs562096361,2
    --109326381(+) AGGGGC/TATATT 1 -- ng310--------
    --
    rs288014811,2
    --109327795(+) TGGGAA/CCCTAG 1 -- ng310--------
    rs285869301,2
    --109296007(+) ACACTA/GTCTAT 1 -- ng510--------
    rs285950301,2
    --109327283(+) CTCCTC/TAGACA 1 -- ng310--------
    --
    rs617549071,2
    --109309974(+) AAAGCC/TTACGC 2 Y/X non10--------
    About this table

    HapMap Linkage Disequilibrium images for ANAPC7 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 606949

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/30 PubMed articles for ANAPC7 gene (see all 30 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 51434 HGNC: 17380 AceView: ARPC3andANAPC7 Ensembl:ENSG00000196510 euGenes: HUgn51434
    ECgene: ANAPC7 H-InvDB: ANAPC7
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ANAPC7:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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