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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ANAPC16 Gene

protein-coding   GIFtS: 43
GCID: GC10P073975

anaphase promoting complex subunit 16

(Previous name: chromosome 10 open reading frame 104 )
(Previous symbol: C10orf104)
  Search for ANAPC16
in our new
 Human Malady Compendium 
Biological research products
for ANAPC16
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3

Aliases
Anaphase Promoting Complex Subunit 161 2     MSAG2 5
C10orf1041 2 3 5     FLJ337281
APC161 2 3     Chromosome 10 Open Reading Frame 1041
CENP-271 2     Anaphase-Promoting Complex Subunit 162
BA570G20.31     Centromere Protein 272
Cyclosome Subunit 162 3     Metabolic Syndrome-Associated Protein2

External Ids:    HGNC: 269761   Entrez Gene: 1195042   Ensembl: ENSG000001662957   OMIM: 6134275   UniProtKB: Q96DE53   
ORGUL members:         
NONCODE:n410929    

Export aliases for ANAPC16 gene to outside databases

Previous GC identifer: GC10P067970


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: APC16_HUMAN, Q96DE5
Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating
ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked
polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000010.10  NC_018921.1  NT_030059.13  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for ANAPC16
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidANAPC16 promoter sequence
   Search SABiosciences Chromatin IP Primers for ANAPC16

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ANAPC16


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q22.1   Ensembl cytogenetic band:  10q22.1   HGNC cytogenetic band: 10q22.2

ANAPC16 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ANAPC16 gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P073975:  view genomic region     (about GC identifiers)

Start:
73,975,787 bp from pter      End:
73,995,618 bp from pter
Size:
19,832 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: APC16_HUMAN, Q96DE5 (See protein sequence)
Recommended Name: Anaphase-promoting complex subunit 16  
Size: 110 amino acids; 11667 Da
Subunit: The APC/C is composed of at least 12 subunits. ANAPC16 associates with the rest of the complex independently
of ANAPC2 and ANAPC11
Subcellular location: Cytoplasm. Nucleus

Explore the universe of human proteins at neXtProt for ANAPC16: NX_Q96DE5

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96DE5

  • ANAPC16 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (4 alternative transcripts): 
    NP_001229475.1  NP_001229476.1  NP_001229477.1  NP_775744.1  

    ENSEMBL proteins: 
     ENSP00000299381  

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    Uscn Proteins for ANAPC16

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005680anaphase-promoting complex IDA--
    GO:0005737cytoplasm IDA--


    ANAPC16 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q96DE5

    UniProtKB/Swiss-Prot: APC16_HUMAN, Q96DE5
    Similarity: Belongs to the APC16 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: APC16_HUMAN, Q96DE5
    Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
    that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating
    ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked
    polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains

    miRNA
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    hsa-miR-548j hsa-miR-579 hsa-miR-607 hsa-miR-520e hsa-miR-605 hsa-miR-374a hsa-miR-219-5p hsa-miR-3921
    SwitchGear 3'UTR luciferase reporter plasmidANAPC16 3' UTR sequence
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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    UniProtKB/Swiss-Prot: APC16_HUMAN, Q96DE5
    Pathway: Protein modification; protein ubiquitination

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ANAPC16

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/29 Interacting proteins for ANAPC16 (Q96DE53 ENSP000002993814) via UniProtKB, MINT, STRING, and/or I2D (see all 29)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    RBBP6Q7Z6E93, ENSP000003178724I2D: score=2 STRING: ENSP00000317872
    ANAPC1Q9H1A43, ENSP000003391094I2D: score=1 STRING: ENSP00000339109
    ANAPC13Q9BS183, ENSP000003469874I2D: score=1 STRING: ENSP00000346987
    ANAPC5Q9UJX43, ENSP000002618194I2D: score=1 STRING: ENSP00000261819
    CDC26Q8NHZ83, ENSP000003633224I2D: score=1 STRING: ENSP00000363322
    About this table

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007067mitosis IEA--
    GO:0016567protein ubiquitination IEA--
    GO:0051301cell division IEA--


    ANAPC16 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ANAPC16
    Search CenterWatch for drugs/clinical trials and news about ANAPC16 / APC16 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ANAPC16 gene (4 alternative transcripts): 
    NM_001242546.1  NM_001242547.1  NM_001242548.1  NM_173473.3  

    Unigene Cluster for ANAPC16:

    Anaphase promoting complex subunit 16
    Hs.426296  [show with all ESTs]
    Unigene Representative Sequence: NM_001242546
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000478193 ENST00000299381(uc001jsw.3 uc001jsv.3 uc021psp.1 uc021psq.1 uc021psr.1 uc021pss.1)
    ENST00000470481(uc021psn.1) ENST00000468144 ENST00000496924

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    8/71 QIAGEN miScript miRNA Assays for microRNAs that regulate ANAPC16 (see all 71):
    hsa-miR-548j hsa-miR-579 hsa-miR-607 hsa-miR-520e hsa-miR-605 hsa-miR-374a hsa-miR-219-5p hsa-miR-3921
    SwitchGear 3'UTR luciferase reporter plasmidANAPC16 3' UTR sequence
    Inhib. RNA
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    Additional cDNA sequence: 

    AF085974.1 AK023981.1 BC009530.1 EU552924.1 NR_038391.1 NR_038392.1 

    24/26 DOTS entries (see all 26):

    DT.100038624  DT.456242  DT.86838897  DT.100038625  DT.121298978  DT.100773647  DT.100822176  DT.92327556 
    DT.100822163  DT.121299050  DT.95156635  DT.91642226  DT.100822175  DT.100822181  DT.429295  DT.100822174 
    DT.121298975  DT.121298980  DT.208458  DT.92449643  DT.92449645  DT.92416030  DT.92449652  DT.121298981 

    24/510 AceView cDNA sequences (see all 510):

    CA866146 AI342713 AA922997 BM998029 CD513155 AA702656 CD724246 AA909205 
    BM698148 AW338672 AI873156 BM670973 CA844207 AA887931 BM667313 BE677674 
    CA867235 CA418323 H83755 AW878580 BF222154 CD675046 BI255936 AV705539 

    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for ANAPC16 (see all 11)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2a · 2b ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b · 5c · 5d · 5e · 5f · 5g · 5h
    SP1:                                      -     -     -     -           -     -                                                               
    SP2:                                -     -     -     -     -           -     -                                                               
    SP3:                                            -     -     -           -     -                                                               
    SP4:                                                        -           -     -                                                               
    SP5:                                -     -     -     -     -     -     -     -                                                               


    ECgene alternative splicing isoforms for ANAPC16

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ANAPC16 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CCAAATGCTG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ANAPC16 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ANAPC16

    SOURCE GeneReport for Unigene cluster: Hs.426296
        SABiosciences Custom PCR Arrays for ANAPC16
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for ANAPC16 gene from 5/12 species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Anapc161 , 5 anaphase promoting complex subunit 161, 5 90(n)1
    100(a)1
      10 (29.66 cM)5
    527171  NM_025514.21  NP_079790.11 
     599879095 
    chicken
    (Gallus gallus)
    Aves ANAPC161 anaphase promoting complex subunit 16 82.12(n)
    99.09(a)
      423708  XM_421587.3  XP_421587.2 
    lizard
    (Anolis carolinensis)
    Reptilia ANAPC166
    --
    96(a)
    1 ↔ 1
    3(51634396-51643382)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.75412 Xenopus laevis transcribed sequence with strong similarity more 81.84(n)    CF547953.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.139492 Transcribed sequence with moderate similarity to protein more 76.6(n)    BI868103.1 


    ENSEMBL Gene Tree for ANAPC16 (if available)
    TreeFam Gene Tree for ANAPC16 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/319 NCBI SNPs in ANAPC16 are shown (see all 319    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 10 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1881081791,2
    --73978923(+) ACTGAC/TGGGAA 6 -- int10--------
    rs798150521,2
    --73979085(+) GTGTAG/ATCCGG 6 -- int11Minor allele frequency- A:0.01NA 120
    rs1512990671,2
    --73979089(+) AGTCCA/GGAAGA 6 -- int10--------
    rs1808852891,2
    --73979127(+) GTTGCC/TTCTGT 6 -- int10--------
    rs1439376771,2
    --73979265(+) GAGGT-/GGGA  
            
    GGATC
    6 -- int10--------
    rs1504713451,2
    --73979353(+) AGTGGA/GGTGTT 6 -- int10--------
    rs1383347891,2
    --73979364(+) CTGGCA/GCGTGC 6 -- int10--------
    rs1862957751,2
    --73979446(+) GCCAGA/GATAGT 6 -- int10--------
    rs1912718921,2
    --73979447(+) CCAGGA/GTAGTG 6 -- int10--------
    rs1835368251,2
    --73979493(+) CTGTCC/TCCAAA 6 -- int10--------

    HapMap Linkage Disequilibrium report for ANAPC16 (73975787 - 73995618 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for ANAPC16: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ANAPC16 for disorders           About MalaCards

    ANAPC16 for disorders           About GeneDecksing

    OMIM gene information: 613427    OMIM disorders: --


    Export disorders for ANAPC16 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ANAPC16 gene, integrated from 9 sources (see all 18):
    (articles sorted by number of sources associating them with ANAPC16)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Systematic analysis of human protein complexes identi fies chromosome segregation proteins. (PubMed id 20360068)1, 2, 3 Hutchins J.R....Peters J.M. (2010)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 3 Ota T.... Sugano S. (2004)
    4. The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)1, 2 Deloukas P.... Rogers J. (2004)
    5. Charting the landscape of tandem BRCT domain-mediated protein interactions. (PubMed id 22990118)1 Woods N.T....Monteiro A.N. (2012)
    6. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    7. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
    8. Lesson from the stoichiometry determination of the coh esin complex: a short protease mediated elution increases the recovery from cros s-linked antibody-conjugated beads. (PubMed id 21043528)1 Holzmann J....Mechtler K. (2011)
    9. BubR1 blocks substrate recruitment to the APC/C in a KEN-box-dependent manner. (PubMed id 22193957)1 Lara-Gonzalez P....Taylor S.S. (2011)
    10. UBE2S drives elongation of K11-linked ubiquitin chain s by the anaphase-promoting complex. (PubMed id 20080579)1 Wu T....Kirschner M.W. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 119504 HGNC: 26976 AceView: C10orf104 Ensembl:ENSG00000166295 euGenes: HUgn119504
    ECgene: ANAPC16 H-InvDB: ANAPC16

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ANAPC16 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ANAPC16 gene:
    Search GeneIP for patents involving ANAPC16

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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