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AMTN Gene

protein-coding GIFtS: 38

GC04P071419

amelotin
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
MGC148132 ^²
MGC148133 ^²
RSTI689 ^¹, ²
UNQ689 ^¹, ²
amelotin ^²

External Ids
HGNC: 33188¹
Entrez Gene: 401138²
UniProtKB: Q6UX39³
Ensembl: ENSG00000187689⁷

Search outside databases for aliases for AMTN gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for AMTN:

The mineralized portions of teeth, the dentin and enamel, are formed by mesenchyme-derived

odontoblasts and epithelium-derived ameloblasts, respectively. As ameloblasts differentiate, they

deposit specific proteins necessary for enamel formation, including amelogenin (AMELX; MIM

300391), enamelin (ENAM; MIM 606585), and ameloblastin (AMBN; MIM 601259), in the organic enamel

matrix. Amelotin is specifically expressed in maturation-stage ameloblasts (Iwasaki et al., 2005

[PubMed 16304441]).[supplied by OMIM]

UniProtKB/Swiss-Prot: AMTN_HUMAN, Q6UX39

Function: Could be a cell adhesion protein involved in the maturation of tooth enamel

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q13.3 Ensembl cytogenetic band: 4q13.3 HGNC cytogenetic band: 4q13.3

AMTN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4 GeneLoc Exon Structure

GeneLoc location for GC04P071419: (about GC identifiers)

Start:	71,418,887 bp from pter
End:	71,433,048 bp from pter
Size:	14,162 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000004.10 NT_077444.3

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: AMTN_HUMAN, Q6UX39 (See protein sequence)

Recommended Name: Amelotin precursor

Size: 209 amino acids; 21588 Da

Subcellular location: Secreted (Probable)

Secondary accessions: Q0P503 Q0P506

Alternative splicing: 2 isoforms: Q6UX39-1 Q6UX39-2

Post-translational modifications:

O-glycosylated (By similarity)¹

REFSEQ proteins: NP_997722.1

ENSEMBL proteins:

ENSP00000341013

Human Recombinant Proteins

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	Human Recombinant Proteins from Abnova (AMTN)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--
GO:0005578	proteinaceous extracellular matrix	ISS	16304441 16787391
GO:0005605	basal lamina	ISS	16787391
GO:0005911	cell-cell junction	ISS	16787391

About this table

Antibodies for AMTN:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for AMTN

Assays for AMTN:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ProtoNet protein and cluster: Q6UX39

UniProtKB/Swiss-Prot: AMTN_HUMAN, Q6UX39

Similarity: Belongs to the amelotin family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (AMTN)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_212557

Applied Biosystems Silencer® siRNAs for AMTN

Sigma-Aldrich siRNA for AMTN

Sigma-Aldrich shRNA Panels and shRNA for AMTN

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_212557
Myc/DDK tagged cDNA clone in CMV expression vector: NM_212557
untagged cDNA clone in CMV expression vector: NM_212557

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_212557

UniProtKB/Swiss-Prot: AMTN_HUMAN, Q6UX39

Function: Could be a cell adhesion protein involved in the maturation of tooth enamel

1 MGI phenotypic allele for Amtn (no phenotypes)

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--
GO:0005515	protein binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

3 Interacting proteins for AMTN (ENSP00000341013³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
AMBN	ENSP00000313809³	STRING (score=.916)
ENAM	ENSP00000254800³	STRING (score=.905)
AMELX	ENSP00000370088³	STRING (score=.877)

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3 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001503	ossification	IEA	--
GO:0007155	cell adhesion	IEA	--
GO:0042475	odontogenesis of dentine-containing tooth	ISS	16304441

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for AMTN

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (AMTN)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_212557

Sigma-Aldrich siRNA for AMTN

Sigma-Aldrich shRNA Panels and shRNA for AMTN

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_212557

REFSEQ mRNAs for AMTN gene:

NM_212557.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_212557

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_212557
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_212557
                                 untagged cDNA clone in CMV expression vector: NM_212557

Additional cDNA sequence:

AY358528.1 BC121812.1 BC121817.1

1 DOTS entry:

DT.97766005

5 AceView cDNA sequences:

BU727294 BM688820 NM_212557 AY358528 BF664579

highest scoring ESTs for AMTN:

AY358528 BU727294 BF664579 BM688820 NM_212557 BC121812 BC121817 XM_371719 XM_376323

Unigene Cluster for AMTN:

Amelotin

Hs.453069 [show with all ESTs]

Unigene Representative Sequence: AY358528

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000339336

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

AMTN expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for AMTN

¹ Data from GNF BioGPS
About these images

About these images

CGAP SAGE TAG: TGAATCTTTA

SOURCE GeneReport for Unigene cluster: Hs.453069

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for AMTN gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	AMTN¹	--	amelotin	80.65(n) 70.73(a)	611794 XM_849506.1 XP_854599.1
chimpanzee (Pan troglodytes)	AMTN¹	--	amelotin	98.88(n) 98.09(a)	742016 XM_001160426.1 XP_001160426.1
cow (Bos taurus)	AMTN¹	--	amelotin	82.29(n) 72.46(a)	618393 XM_870725.2 XP_875818.2
rat (Rattus norvegicus)	Amtn¹	--	amelotin	75.45(n) 61.46(a)	689404 XM_001070666.1 XP_001070666.1
mouse (Mus musculus)	Amtn^{1, 5}	5⁵	amelotin^{1, 5}	76.6(n)¹ 61.06(a)¹	71421¹ XM_284166.3¹ XP_284166.1¹ AK017352⁵

About this table Species with no ortholog for AMTN

ENSEMBL Gene Tree for AMTN

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/148 NCBI SNPs in AMTN are shown (see all 148 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 56)

AB	SNP ID	Valid	Chr 4 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
--	rs7660807^1,2	C,F,H	71423140(+)	`GTCAAA/GTCAGG`	1	N/S	mis¹		9	EU EA WA NA	872
	rs34803339^1,2	C,F	71424183(+)	`TTCCTT/CCTTTA`	1	P/S	mis¹		5	NA EU EA WA	470
--	rs17149014^1,2	C,F	71433129(+)	`ACTCCA/TGGTCT`	1	--	ng3¹		3	NA	142
	rs17676820^1,2	C,F,H	71431578(+)	`GCCATC/TGAGGA`	1	I/I	syn¹		16	NA EU EA WA	1418
	rs17149007^1,2	C,F,H	71424526(+)	`CCTTCC/TTTAAT`	1	--	int¹		11	NA EU EA WA	956
--	rs17149008^1,2	C,F,H	71428347(+)	`AAATAT/CCCATC`	1	--	int¹		11	NA EU EA WA	922
	rs17149012^1,2	C,F,H	71430111(+)	`ATTGAG/AGCAAT`	1	--	int¹		7	NA EU EA WA	558
	rs17733431^1,2	C,F,H	71430084(+)	`GATATC/ATAAAC`	1	--	int¹		6	NA EU EA	438
--	rs3852099^1,2	C,F,H	71432653(+)	`AGAATG/AATATG`	1	--	int¹		7	EU EA WA NA	560
--	rs17733287^1,2	C,F,H	71425384(+)	`ACTGAC/AGAATG`	1	--	int¹		7	NA EU EA WA	538

About this table

HapMap Linkage Disequilibrium images for AMTN (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 610912

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

7 PubMed articles for AMTN gene:

Amelotin--a Novel Secreted, Ameloblast-specific Protein. (PubMed id 16304441)^{2, 3} Iwasaki K....Ganss B. (2005)
Signal peptide prediction based on analysis of experimentally verified cleavage sites. (PubMed id 15340161)^{3, 4} Zhang Z. and Henzel W.J. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)^{3, 4} Clark H.F....Gray A. (2003)
Cloning of rat amelotin and localization of the protein to the basal lamina of maturation stage ameloblasts and junctional epithelium. (PubMed id 16787391)³ Moffatt P....Nanci A. (2006)
The consensus coding sequences of human breast and colorectal cancers. (PubMed id 16959974)⁴ Sjoeblom T.... Velculescu V.E. (2006)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 401138	HGNC: 33188	AceView: UNQ689	Ensembl:ENSG00000187689	euGenes: HUgn401138
ECgene: AMTN	H-InvDB: AMTN

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for AMTN
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for AMTN:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for AMTN-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for AMTN		Browse antibodies at Sigma-Aldrich
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	Lentivirus-delivered shRNAs for AMTN		Browse small molecules at Sigma-Aldrich
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	Antibodies		Primer Pairs
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	Recombinant & Natural Proteins

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AMTN Gene

protein-coding GIFtS: 38

Aliases &
Descriptions
for AMTN

Summaries
for AMTN

Genomic Location
for AMTN

Proteins
for AMTN

Protein Domains/
Families
for AMTN

Gene Function
for AMTN

Pathways & Interactions
for AMTN

Drugs & Compounds
for AMTN

Transcripts
for AMTN

Expression
for AMTN

Orthologs
for AMTN

Paralogs
for AMTN

SNPs/Variants
for AMTN

Disorders & Mutations
for AMTN

Medical News
for AMTN

Publications
for AMTN

Search for AMTN

Genome Databases showing AMTN

Other Databases showing AMTN

Specialized Databases showing AMTN

Licensable Technologies
for AMTN

Services
for AMTN

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

AMTN Gene

protein-coding GIFtS: 38

Aliases & Descriptionsfor AMTN

Summariesfor AMTN

Genomic Locationfor AMTN

Proteinsfor AMTN

Protein Domains/Familiesfor AMTN

Gene Functionfor AMTN

Pathways & Interactionsfor AMTN

Drugs & Compoundsfor AMTN

Transcriptsfor AMTN

Expression for AMTN

Orthologsfor AMTN

Paralogsfor AMTN

SNPs/Variantsfor AMTN

Disorders & Mutationsfor AMTN

Medical Newsfor AMTN

Publicationsfor AMTN

Search for AMTN

Genome Databases showing AMTN

Other Databases showing AMTN

Specialized Databases showing AMTN

Licensable Technologiesfor AMTN

Servicesfor AMTN

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for AMTN

Summaries
for AMTN

Genomic Location
for AMTN

Proteins
for AMTN

Protein Domains/
Families
for AMTN

Gene Function
for AMTN

Pathways & Interactions
for AMTN

Drugs & Compounds
for AMTN

Transcripts
for AMTN

Expression
for AMTN

Orthologs
for AMTN

Paralogs
for AMTN

SNPs/Variants
for AMTN

Disorders & Mutations
for AMTN

Medical News
for AMTN

Publications
for AMTN

Licensable Technologies
for AMTN

Services
for AMTN