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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

AMFR Gene

protein-coding GIFtS: 62
GCID: GC16M056395

autocrine motility factor receptor, E3 ubiquitin protein...

(Previous name: autocrine motility factor receptor )

Explore 36 diseases affiliated with
AMFR via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Autocrine Motility Factor Receptor, E3 Ubiquitin Protein Ligase¹ ²		GP78² ⁵
RNF45¹ ² ³		E3 Ubiquitin-Protein Ligase AMFR²
Autocrine Motility Factor Receptor¹ ³		EC 6.3.2.-³
Gp78¹		AMF Receptor³
RING Finger Protein 45² ³

External Ids:	HGNC: 463¹	Entrez Gene: 267²	Ensembl: ENSG00000159461⁷	OMIM: 603243⁵	UniProtKB: Q9UKV5³

Export aliases for AMFR gene to outside databases

Previous GC identifers: GC16M046778 GC16M056447 GC16M056130 GC16M056170 GC16M054952 GC16M042266

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for AMFR:

This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor

motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase

family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.

(provided by RefSeq, Feb 2012)

UniProtKB/Swiss-Prot: AMFR_HUMAN, Q9UKV5

Function: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D,

CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the

final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the

sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and

initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the

cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination,

retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine

motility factor

Gene Wiki entry for AMFR

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000016.9 NC_018927.1 NT_010498.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the AMFR gene promoter:
TBP AML1a AP-4 NF-AT Roaz RORalpha1 Nkx6-1 COMP1 STAT3 NF-AT1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: AMFR promoter sequence

Search SABiosciences Chromatin IP Primers for AMFR

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AMFR

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q21 Ensembl cytogenetic band: 16q12.2 HGNC cytogenetic band: 16q21

AMFR Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AMFR gene location

GeneLoc information about chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16M056395: view genomic region (about GC identifiers)

Start:	56,395,364 bp from pter	End:	56,459,450 bp from pter
Size:	64,087 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: AMFR_HUMAN, Q9UKV5 (See protein sequence)

Recommended Name: E3 ubiquitin-protein ligase AMFR

Size: 643 amino acids; 72996 Da

Subunit: Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required

for coupling retrotranslocation, ubiquitination and deglycosylation (By similarity). Interacts with DRL1. Interacts

(through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that

enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated

degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the

interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein

Sequence caution: Sequence=AAA36671.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors;

Sequence=AAA79362.1; Type=Frameshift; Positions=355, 388, 411, 487, 537, 583, 632;

6/9 PDB 3D structures from and Proteopedia for AMFR (see all 9):

2EJS (3D)

2LVN (3D)

2LVO (3D)

2LVP (3D)

2LVQ (3D)

3FSH (3D)

Secondary accessions: P26442 Q8IZ70

Explore the universe of human proteins at neXtProt for AMFR: NX_Q9UKV5

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q9UKV5

AMFR Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_001135.3

ENSEMBL proteins:

ENSP00000290649 ENSP00000456288 ENSP00000456745 ENSP00000455842

Human Recombinant Protein Products for AMFR:

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	Novus Biologicals AMFR Protein Novus Biologicals AMFR Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for AMFR

Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	--	--
GO:0005737	cytoplasm	--	--
GO:0016021	integral to membrane	NAS	1649192
GO:0030176	integral to endoplasmic reticulum membrane	IDA	11724934
GO:0030425	dendrite	--	--

AMFR for ontologies About GeneDecksing

AMFR Antibody Products:

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	Novus Biologicals AMFR Antibodies
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Assay Products for AMFR:

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	Uscn ELISAs and CLIAs for AMFR

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

AMFR for domains About GeneDecksing

4 InterPro domains/families:

IPR001841 Znf_RING

IPR013083 Znf_RING/FYVE/PHD

IPR026608 Amfr

IPR003892 CUE

Graphical View of Domain Structure for InterPro Entry Q9UKV5

ProtoNet protein and cluster: Q9UKV5

2 Blocks protein families:

IPB001841 Zn-finger

IPB003892 Ubiquitin system component Cue

UniProtKB/Swiss-Prot: AMFR_HUMAN, Q9UKV5

Domain: The VCP/p97-interacting motif (VIM) is sufficient for binding VCP/p97 to form a complex capable of transfering

VCP/p97 from the cytosol to microsomes

Similarity: Contains 1 CUE domain

Similarity: Contains 1 RING-type zinc finger

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: AMFR_HUMAN, Q9UKV5

Function: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D,

CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the

final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the

sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and

initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the

cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination,

retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine

motility factor

Genatlas biochemistry entry for AMFR:

autocrine motility factor receptor

Enzyme Number (IUBMB): EC 6.3.2.-¹

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004842	ubiquitin-protein ligase activity	IMP	16168377
GO:0004872	receptor activity	IPI	1649192
GO:0005515	protein binding	IPI	16275660
GO:0008270	zinc ion binding	IEA	--

AMFR for ontologies About GeneDecksing

Phenotypes:
3 GenomeRNAi human phenotypes for AMFR:

Decreased focal adhesion (FA)

Increased Salmonella enterica

Synthetic lethal with Ras

Animal Models:

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for AMFR

miRNA Products:		OriGene 3'-UTR Clone: AMFR Browse MicroRNA Expression Plasmids
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		hsa-miR-429 hsa-miR-29c hsa-miR-29a hsa-miR-4263 hsa-miR-4275 hsa-miR-548l hsa-miR-548a-3p hsa-miR-7-1*

		SwitchGear 3'UTR luciferase reporter plasmid: AMFR 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
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In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AMFR

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Protein processing in endoplasmic reticulum

Protein processing in endoplasmic reticulum

1.00

Protein Stability

Protein Stability

1.00

Regulation of degradation of wt-CFTR

Regulation of degradation of deltaF508 CFTR in CF

0.59

Ubiquitinated Orc1 is degraded by the proteasome

SREBP Proteolysis

0.40

Pathway sources
See GeneCards unified pathways
Show all pathways

1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for AMFR

SREBP Proteolysis

1 Cell Signaling Technology (CST) Pathway for AMFR

Protein Stability

1 GeneGo (Thomson Reuters) Pathway for AMFR

Regulation of degradation of deltaF508 CFTR in CF

1 Kegg Pathway (Kegg details for AMFR):

Protein processing in endoplasmic reticulum

UniProtKB/Swiss-Prot: AMFR_HUMAN, Q9UKV5

Pathway: Protein modification; protein ubiquitination

AMFR for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for AMFR

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/74 Interacting proteins for AMFR (Q9UKV5^{1, 2, 3} ENSP00000290649⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 74)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
VCP	P55072¹^,³, ENSP00000351777⁴	EBI-1046379,EBI-1046421 I2D: score=5 STRING: ENSP00000351777
CRMP1	Q14194²^,³, ENSP00000321606⁴	MINT-8247685 I2D: score=2 STRING: ENSP00000321606
TOM1L1	O75674²^,³, ENSP00000408958⁴	MINT-8247696 I2D: score=2 STRING: ENSP00000408958
UBE2D2	P62837³, ENSP00000381717⁴	I2D: score=4 STRING: ENSP00000381717
UBE2G2	P60604³, ENSP00000338348⁴	I2D: score=3 STRING: ENSP00000338348

About this table

Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000209	protein polyubiquitination	IDA	19103148
GO:0006511	ubiquitin-dependent protein catabolic process	IMP	16168377
GO:0006928	cellular component movement	TAS	1649192
GO:0007165	signal transduction	TAS	1649192
GO:0007568	aging	--	--

AMFR for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

AMFR for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for AMFR

4 HMDB Compounds for AMFR About this table

Compound	Synonyms	CAS #	PubMed Ids
Adenosine monophosphate	5'-AMP (see all 28)	61-19-8	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Phosphoric acid	acide phosphorique (FRENCH) (see all 20)	7664-38-2	--
Pyrophosphate	(4-)Diphosphoric acid ion (see all 10)	14000-31-8	--

6 Novoseek chemical compound relationships for AMFR gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
ilimaquinone	77.2	13	9365274 (6), 16709164 (1)
nocodazole	33.6	2	9365274 (1)
vegf	15.3	5	11269478 (3), 17115102 (1), 10800788 (1)
cholesterol	4.6	4	17681147 (2), 11724808 (1)
estrogen	0	1	10545751 (1)
retinoic acid	0	2	7895580 (2)

Search CenterWatch for drugs/clinical trials and news about AMFR

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for AMFR gene (2 alternative transcripts):

NM_001144.5 NM_138958.1

Unigene Cluster for AMFR:

Autocrine motility factor receptor, E3 ubiquitin protein ligase

Hs.295137 [show with all ESTs]

Unigene Representative Sequence: NM_001144

12 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000290649(uc002eix.3 uc002eiy.3) ENST00000492830 ENST00000566757

ENST00000568325 ENST00000563285 ENST00000567738 ENST00000566334 ENST00000568762

ENST00000565445 ENST00000563664 ENST00000568657 ENST00000564283

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		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat AMFR
		8/30 QIAGEN miScript miRNA Assays for microRNAs that regulate AMFR (see all 30):
		hsa-miR-429 hsa-miR-29c hsa-miR-29a hsa-miR-4263 hsa-miR-4275 hsa-miR-548l hsa-miR-548a-3p hsa-miR-7-1*

		SwitchGear 3'UTR luciferase reporter plasmid: AMFR 3' UTR sequence

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Additional cDNA sequence:

AF124145.1 AK023874.1 AK123962.1 BC017043.1 BC051032.1 BC056869.1 BC069197.1 BC153819.1

L35233.1 M63175.1

14 DOTS entries:

DT.92450014 DT.100798860 DT.92450015 DT.101983903 DT.40307474 DT.92450022 DT.100798870 DT.100798868

DT.100709573 DT.455385 DT.87016079 DT.92450016 DT.95334745 DT.95348055

24/288 AceView cDNA sequences (see all 288):

BC017043 BM818525 AW016513 BM475584 BM761427 AA813969 AW628736 BF348165

BM700037 CB216174 CA314535 CA425340 AI813343 AI912847 AA653080 AA280077

BM475770 AW675386 BE712868 BM844960 CA426429 BC056869 H02984 BM977340

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for AMFR About this scheme

ExUns:	1	^	2	^	3	^	4	^	5	^	6	^	7	^	8a	·	8b	^	9	^	10a	·	10b	^	11	^	12	^	13	^	14a	·	14b
SP1:																							-
SP2:																			-				-
SP3:																			-

ECgene alternative splicing isoforms for AMFR

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

AMFR expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AAGTCATAGG

About this image

AMFR expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

1 LifeMap In Vivo Development Anatomical Compartment/Cell

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Liver

Liver Lobule

Liver

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See AMFR Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for AMFR

SOURCE GeneReport for Unigene cluster: Hs.295137

SABiosciences Expression via Pathway-Focused PCR Arrays including AMFR:

Unfolded Protein Response in human mouse rat

Ubiquitin Ligases in human mouse rat

Molecular Toxicology PathwayFinder 384HT in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for AMFR Browse OriGene validated miRNA SYBR primer pairs
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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat AMFR

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AMFR

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for AMFR gene from 9/27 species (see all 27) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Amfr^{1 , 5}	autocrine motility factor receptor^{1, 5}	88.78(n)¹ 94.68(a)¹		8 (45.96 cM)⁵ 23802¹ NM_011787.2¹ NP_035917.2¹ 93971588⁵
chicken (Gallus gallus)	Aves	AMFR¹	autocrine motility factor receptor	79.53(n) 87.78(a)		415697 XM_414064.3 XP_414064.3
lizard (Anolis carolinensis)	Reptilia	AMFR⁶	--	86(a)	1 ↔ 1	GL343675.1(60632-85074)
African clawed frog (Xenopus laevis)	Amphibia	MGC68459²	hypothetical protein MGC68459	79.15(n)		BC060333.1
zebrafish (Danio rerio)	Actinopterygii	Dr.19542²	Danio rerio cDNA clone MGC63893 IMAGE6789887, complete cds less	77.29(n)		BC057411.1
mosquito (Anopheles gambiae)	Insecta	AgaP_AGAP007538¹	AGAP007538-PA	55.51(n) 48.97(a)		4576491 XM_001237061.2 XP_001237062.2
worm (Caenorhabditis elegans)	Secernentea	hrdl-1¹	Protein HRDL-1	39.44(n) 25.98(a)		172833 NM_060201.3 NP_492602.1
thale cress (Arabidopsis thaliana)	eudicotyledons	RIN2⁶ RIN3⁶	E3 ubiquitin protein ligase RIN3	18(a) 17(a)	many → 1 many → 1	4(12923919-12929156) 5(20892643-20897817)
rice (Oryza sativa)	Liliopsida	--	RIN3, putative, expressed	17(a)	1 ↔ 1	7(17743008-17754353)

ENSEMBL Gene Tree for AMFR (if available)
TreeFam Gene Tree for AMFR (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for AMFR gene

SYVN1² RNF139² RNF145²

3 SIMAP similar genes for AMFR using alignment to 6 protein entries: AMFR_HUMAN (see all proteins):

TRIM3 RAD18 TRIM39

AMFR for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 16 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs2617845^1,2	C,F,H	--	56394937(+)	`TGCTCT/CAAACA`	1	--	ds500¹		5		NS EA WA	522
rs144396200^1,2	C	--	56394956(+)	`GGCTG-/GGT TTTTTTTTTG`	1	--	ds500¹		0	--	--	--	--
rs189308209^1,2		--	56395034(+)	`ACATGC/TGGAAT`	1	--	ds500¹		0	--	--	--	--
rs181294366^1,2		--	56395085(+)	`TAATTC/TGAGGA`	1	--	ds500¹		0	--	--	--	--
rs149788614^1,2		--	56395107(+)	`TCTTTC/TGCTAC`	1	--	ds500¹		0	--	--	--	--
rs139897271^1,2		--	56395123(+)	`TACCT-/GAAAAA`	1	--	ds500¹		0	--	--	--	--
rs113127022^1,2	C	--	56395124(+)	`ACCTG-/AAAAAA`	1	--	ds500¹		1		CSA	2
rs28506013^1,2	F	--	56395379(+)	`TTTTTT/ATTATT`	1	--	ut3¹		1		NA	4
rs118005810^1,2	F	--	56395447(+)	`AAAAAT/ACACAG`	1	--	ut3¹		1		NA	120
rs200174342^1,2		--	56395447(+)	`AAAAA-/TCACAG`	1	--	ut3¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for AMFR (56395364 - 56459450 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for AMFR
1 CNV: 4957
1 Indel: 72590

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

AMFR for disorders           About GeneDecksing

OMIM gene information: 603243    OMIM disorders: --

20/36 diseases for AMFR (see all 36):    About MalaCards

japanese encephalitis cutaneous malignant melanoma esophageal squamous cell carcinoma squamous cell carcinoma

giant cell tumor adult t-cell leukemia transitional cell carcinoma bladder carcinoma

fibrosarcoma encephalitis cystic fibrosis carcinoma

hepatocellular carcinoma t-cell leukemia gastric cancer thymoma

colorectal cancer breast cancer endometrial cancer sinusitis

1 disease from the University of Copenhagen DISEASES database for AMFR:

Infertility

10/17 Novoseek disease relationships for AMFR gene (see all 17) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
metastasis	52.6	33	11724934 (2), 8980404 (2), 18037895 (2), 12209971 (2) (see all 20)
tumors	47.2	72	12962414 (4), 11668522 (4), 11269478 (3), 7591303 (2) (see all 30)
bladder carcinoma	38.1	4	8205527 (3), 9176385 (1)
fibrosarcoma	20.1	5	1649192 (2), 14968121 (1)
melanoma	20	4	2153051 (1), 9533771 (1), 12563351 (1), 8832943 (1)
cancer lung	19.2	11	11748469 (4), 12962414 (3), 11668522 (2)
carcinoma transitional cell	17.8	3	7490884 (2), 14685796 (1)
bladder cancer	17.3	1	8205527 (1)
nsclc	12.8	13	12962414 (3), 11668522 (3), 11269478 (2)
carcinoma squamous cell	0	5	7591303 (3)

Export disorders for AMFR gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for AMFR gene, integrated from 9 sources (see all 137):
(articles sorted by number of sources associating them with AMFR)

Utopia: connect your pdf to the dynamic
world of online information

Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor. (PubMed id 1649192)^{1, 2, 3, 9} Watanabe H....Raz A. (1991)
The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. (PubMed id 11724934)^{1, 2, 9} Fang S.... Weissman A.M. (2001)
The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein. (PubMed id 10456327)^{1, 2, 9} Shimizu K.... Yokota J. (1999)
CYP3A4 ubiquitination by gp78 (the tumor autocrine motility factor receptor, AMFR) and CHIP E3 ligases. (PubMed id 19103148)^{1, 2, 9} Pabarcus M.K....Correia M.A. (2009)
Overexpression of the tumor autocrine motility factor receptor, gp78, a ubiquitin protein ligase (E3), results in increased ubiquitinylation and decreased secretion of apolipoprotein B100 in Hep G2 cells. (PubMed id 12670940)^{1, 2, 9} Liang J.S....Ginsberg H.N. (2003)
Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase. (PubMed id 16168377)^{1, 2, 9} Song B.L....DeBose-Boyd R.A. (2005)
Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor. (PubMed id 7626106)^{1, 2, 9} Huang B.... Raz A. (1995)
Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane. (PubMed id 16186510)^{1, 2} Ye Y....Rapoport T.A. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Autocrine motility factor-receptor gene expression in lung cancer. (PubMed id 12962414)^{1, 9} Takanami I. and Takeuchi K. (2003)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 267	HGNC: 463	AceView: AMFR	Ensembl:ENSG00000159461	euGenes: HUgn267
ECgene: AMFR	Kegg: 267	H-InvDB: AMFR

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for AMFR	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for AMFR	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for AMFR gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for AMFR gene:
Search GeneIP for patents involving AMFR

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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