Free for academic non-profit institutions. Other users need a Commercial license

Aliases for AMFR Gene

Aliases for AMFR Gene

  • Autocrine Motility Factor Receptor, E3 Ubiquitin Protein Ligase 2 3
  • GP78 3 4 6
  • Autocrine Motility Factor Receptor 2 4
  • RING Finger Protein 45 3 4
  • RNF45 3 4
  • E3 Ubiquitin-Protein Ligase AMFR 3
  • AMF Receptor 4
  • EC 6.3.2.- 4

External Ids for AMFR Gene

Previous GeneCards Identifiers for AMFR Gene

  • GC16M046778
  • GC16M056447
  • GC16M056130
  • GC16M056170
  • GC16M054952
  • GC16M042266
  • GC16M056395

Summaries for AMFR Gene

Entrez Gene Summary for AMFR Gene

  • This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]

GeneCards Summary for AMFR Gene

AMFR (Autocrine Motility Factor Receptor, E3 Ubiquitin Protein Ligase) is a Protein Coding gene. Diseases associated with AMFR include colorectal cancer. Among its related pathways are Regulation of Cholesterol Biosynthesis by SREBP (SREBF) and Protein Stability. GO annotations related to this gene include ubiquitin-protein transferase activity and receptor activity. An important paralog of this gene is RNF145.

UniProtKB/Swiss-Prot for AMFR Gene

  • E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.

Gene Wiki entry for AMFR Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AMFR Gene

Genomics for AMFR Gene

Regulatory Elements for AMFR Gene

Genomic Location for AMFR Gene

Start:
56,361,452 bp from pter
End:
56,425,538 bp from pter
Size:
64,087 bases
Orientation:
Minus strand

Genomic View for AMFR Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for AMFR Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AMFR Gene

Proteins for AMFR Gene

  • Protein details for AMFR Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UKV5-AMFR_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase AMFR
    Protein Accession:
    Q9UKV5
    Secondary Accessions:
    • P26442
    • Q8IZ70

    Protein attributes for AMFR Gene

    Size:
    643 amino acids
    Molecular mass:
    72996 Da
    Quaternary structure:
    • Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation (By similarity). Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway.
    SequenceCaution:
    • Sequence=AAA36671.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors.; Evidence={ECO:0000305}; Sequence=AAA79362.1; Type=Frameshift; Positions=355, 388, 411, 487, 537, 583, 632; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for AMFR Gene

neXtProt entry for AMFR Gene

Proteomics data for AMFR Gene at MOPED

Post-translational modifications for AMFR Gene

  • Ubiquitination at Lys600
  • Modification sites at PhosphoSitePlus

Other Protein References for AMFR Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for AMFR (AMFR)

No data available for DME Specific Peptides for AMFR Gene

Domains for AMFR Gene

Gene Families for AMFR Gene

HGNC:
  • RNF :RING-type (C3HC4) zinc fingers

Protein Domains for AMFR Gene

Suggested Antigen Peptide Sequences for AMFR Gene

Graphical View of Domain Structure for InterPro Entry

Q9UKV5

UniProtKB/Swiss-Prot:

AMFR_HUMAN :
  • Q9UKV5
Domain:
  • The VCP/p97-interacting motif (VIM) is sufficient for binding VCP/p97 to form a complex capable of transferring VCP/p97 from the cytosol to microsomes
  • Contains 1 CUE domain.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with AMFR: view

Function for AMFR Gene

Molecular function for AMFR Gene

GENATLAS Biochemistry: autocrine motility factor receptor
UniProtKB/Swiss-Prot Function: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.

Enzyme Numbers (IUBMB) for AMFR Gene

Gene Ontology (GO) - Molecular Function for AMFR Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IMP 16168377
GO:0004872 receptor activity IPI 1649192
GO:0005515 protein binding IPI 11724934
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
genes like me logo Genes that share ontologies with AMFR: view

Phenotypes for AMFR Gene

genes like me logo Genes that share phenotypes with AMFR: view

Animal Models for AMFR Gene

MGI Knock Outs for AMFR:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for AMFR

In Situ Assay Products

Flow Cytometry Products

No data available for miRNA , Transcription Factor Targeting and HOMER Transcription for AMFR Gene

Localization for AMFR Gene

Subcellular locations from UniProtKB/Swiss-Prot for AMFR Gene

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for AMFR Gene COMPARTMENTS Subcellular localization image for AMFR gene
Compartment Confidence
endoplasmic reticulum 5
plasma membrane 3
cytosol 2
extracellular 2
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for AMFR Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000836 Hrd1p ubiquitin ligase complex IDA 17872946
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm --
GO:0005789 endoplasmic reticulum membrane IDA 17872946
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with AMFR: view

Pathways for AMFR Gene

genes like me logo Genes that share pathways with AMFR: view

Pathways by source for AMFR Gene

1 Qiagen pathway for AMFR Gene
1 Cell Signaling Technology pathway for AMFR Gene
1 BioSystems pathway for AMFR Gene
1 KEGG pathway for AMFR Gene
1 GeneGo (Thomson Reuters) pathway for AMFR Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

UniProtKB/Swiss-Prot Q9UKV5-AMFR_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for AMFR Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000209 protein polyubiquitination IDA 17310145
GO:0006511 ubiquitin-dependent protein catabolic process IMP 16168377
GO:0006928 movement of cell or subcellular component TAS 1649192
GO:0007165 signal transduction TAS 1649192
GO:0007568 aging IEA --
genes like me logo Genes that share ontologies with AMFR: view

Compounds for AMFR Gene

(4) HMDB Compounds for AMFR Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine monophosphate
  • 5'-AMP
61-19-8
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
Phosphoric acid
  • acide phosphorique (FRENCH)
7664-38-2
Pyrophosphate
  • (4-)Diphosphoric acid ion
14000-31-8

(6) Novoseek inferred chemical compound relationships for AMFR Gene

Compound -log(P) Hits PubMed IDs
ilimaquinone 77.2 7
nocodazole 33.6 1
vegf 15.3 5
cholesterol 4.6 3
estrogen 0 1
genes like me logo Genes that share compounds with AMFR: view

Transcripts for AMFR Gene

Unigene Clusters for AMFR Gene

Autocrine motility factor receptor, E3 ubiquitin protein ligase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for AMFR

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for AMFR Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14a · 14b
SP1: -
SP2: - -
SP3: -

Relevant External Links for AMFR Gene

GeneLoc Exon Structure for
AMFR
ECgene alternative splicing isoforms for
AMFR

Expression for AMFR Gene

mRNA expression in normal human tissues for AMFR Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for AMFR Gene

This gene is overexpressed in Muscle - Skeletal (4.8).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for AMFR Gene

SOURCE GeneReport for Unigene cluster for AMFR Gene Hs.295137

genes like me logo Genes that share expressions with AMFR: view

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for AMFR Gene

Orthologs for AMFR Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for AMFR Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia AMFR 35
  • 99.82 (n)
  • 100 (a)
AMFR 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia AMFR 35
  • 89.99 (n)
  • 94.25 (a)
AMFR 36
  • 89 (a)
OneToOne
dog
(Canis familiaris)
Mammalia AMFR 35
  • 89.49 (n)
  • 95.16 (a)
AMFR 36
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Amfr 35
  • 88.78 (n)
  • 94.68 (a)
Amfr 16
Amfr 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 90 (a)
OneToMany
-- 36
  • 72 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia AMFR 36
  • 86 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Amfr 35
  • 86 (n)
  • 91.03 (a)
chicken
(Gallus gallus)
Aves AMFR 35
  • 79.53 (n)
  • 87.78 (a)
AMFR 36
  • 88 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia AMFR 36
  • 86 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia MGC68459 35
tropical clawed frog
(Silurana tropicalis)
Amphibia amfr 35
  • 70.22 (n)
  • 77.74 (a)
Str.7084 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.5600 35
zebrafish
(Danio rerio)
Actinopterygii amfr 35
  • 69.46 (n)
  • 74.06 (a)
AMFR 36
  • 48 (a)
OneToOne
Dr.19542 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007538 35
  • 55.24 (n)
  • 49.06 (a)
worm
(Caenorhabditis elegans)
Secernentea hrdl-1 35
  • 40.92 (n)
  • 27.35 (a)
hrdl-1 36
  • 21 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes HRD1 36
  • 15 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 46 (a)
OneToOne
Species with no ortholog for AMFR:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for AMFR Gene

ENSEMBL:
Gene Tree for AMFR (if available)
TreeFam:
Gene Tree for AMFR (if available)

Paralogs for AMFR Gene

Paralogs for AMFR Gene

Selected SIMAP similar genes for AMFR Gene using alignment to 7 proteins:

genes like me logo Genes that share paralogs with AMFR: view

Variants for AMFR Gene

Sequence variations from dbSNP and Humsavar for AMFR Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs4924 -- 56,362,574(-) CATGC(A/G)TACGT utr-variant-3-prime
rs13468 -- 56,362,192(-) TGGGT(C/T)CCCTC utr-variant-3-prime
rs731118 -- 56,364,207(+) TGGAC(C/G)CAAAG intron-variant
rs731119 -- 56,364,459(+) TCCTG(A/G)GAATT intron-variant
rs899242 -- 56,364,673(-) GGGGA(C/G)GTTGA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for AMFR Gene

Variant ID Type Subtype PubMed ID
nsv833242 CNV Loss 17160897
nsv906714 CNV Loss 21882294
nsv519979 CNV Loss 19592680
nsv906715 CNV Loss 21882294
dgv2870n71 CNV Loss 21882294
nsv525929 CNV Loss 19592680
nsv833243 CNV Gain 17160897
esv2714510 CNV Deletion 23290073
esv29720 CNV Loss 19812545

Relevant External Links for AMFR Gene

HapMap Linkage Disequilibrium report
AMFR

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for AMFR Gene

Disorders for AMFR Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for AMFR Gene including...

Search for AMFR Gene in MalaCards »

(1) University of Copenhagen DISEASES for AMFR Gene

(17) Novoseek inferred disease relationships for AMFR Gene

Disease -log(P) Hits PubMed IDs
metastasis 52.6 26
tumors 47.2 46
bladder carcinoma 38.1 4
fibrosarcoma 20.1 3
melanoma 20 4
genes like me logo Genes that share disorders with AMFR: view

No data available for UniProtKB/Swiss-Prot for AMFR Gene

Publications for AMFR Gene

  1. Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor. (PMID: 1649192) Watanabe H. … Raz A. (J. Biol. Chem. 1991) 2 3 4 23
  2. The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. (PMID: 11724934) Fang S. … Weissman A.M. (Proc. Natl. Acad. Sci. U.S.A. 2001) 3 4 23
  3. The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein. (PMID: 10456327) Shimizu K. … Yokota J. (FEBS Lett. 1999) 3 4 23
  4. Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor. (PMID: 7626106) Huang B. … Raz A. (Biochem. Biophys. Res. Commun. 1995) 3 4 23
  5. Overexpression of the tumor autocrine motility factor receptor, gp78, a ubiquitin protein ligase (E3), results in increased ubiquitinylation and decreased secretion of apolipoprotein B100 in Hep G2 cells. (PMID: 12670940) Liang J.S. … Ginsberg H.N. (J. Biol. Chem. 2003) 3 4 23

Products for AMFR Gene

  • Addgene plasmids for AMFR

Sources for AMFR Gene

Back to Top

Content