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AMER3 Gene

protein-coding   GIFtS: 32
GCID: GC02P131538

APC Membrane Recruitment Protein 3

(Previous name: family with sequence similarity 123C)
(Previous symbol: FAM123C)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
APC Membrane Recruitment Protein 31 2
FAM123C1 2 3
Family With Sequence Similarity 123C1 2
Protein FAM123C2 3
Amer33

External Ids:    HGNC: 267711   Entrez Gene: 2051472   Ensembl: ENSG000001781717   UniProtKB: Q8N9443   

Export aliases for AMER3 gene to outside databases

Previous GC identifer: GC02P131514


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for AMER3 Gene:
AMER3 (APC membrane recruitment protein 3) is a protein-coding gene. GO annotations related to this gene include lipid binding. An important paralog of this gene is AMER2.

UniProtKB/Swiss-Prot: AMER3_HUMAN, Q8N944
Function: Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol
4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000002.11  NC_018913.2  NT_005403.18  
Regulatory elements:
   Search for regulatory transcription factor binding sites for AMER3
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidAMER3 promoter sequence
   Search Chromatin IP Primers for AMER3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat AMER3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q21.1   Ensembl cytogenetic band:  2q21.1   HGNC cytogenetic band: 2q21.1

AMER3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AMER3 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P131538:  view genomic region     (about GC identifiers)

Start:
131,513,008 bp from pter      End:
131,525,707 bp from pter
Size:
12,700 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: AMER3_HUMAN, Q8N944 (See protein sequence)
Recommended Name: APC membrane recruitment protein 3  
Size: 861 amino acids; 90445 Da
Secondary accessions: B7ZLH6

Explore the universe of human proteins at neXtProt for AMER3: NX_Q8N944

Explore proteomics data for AMER3 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See AMER3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001098663.1  NP_001098664.1  NP_001098665.1  NP_689911.2  

    ENSEMBL proteins: 
     ENSP00000314914   ENSP00000410421   ENSP00000389242   ENSP00000392700  

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    antibodies-online kits for AMER3 (4 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR019003 Uncharacterised_FAM123

    Graphical View of Domain Structure for InterPro Entry Q8N944

    ProtoNet protein and cluster: Q8N944

    UniProtKB/Swiss-Prot: AMER3_HUMAN, Q8N944
    Similarity: Belongs to the Amer family


    Find genes that share domains with AMER3           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: AMER3_HUMAN, Q8N944
    Function: Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol
    4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane (By similarity)

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI--
    GO:0008289lipid binding IEA--
         
    Find genes that share ontologies with AMER3           About GenesLikeMe


    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for AMER3

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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    AMER3_HUMAN, Q8N944: Cell membrane; Peripheral membrane protein (By similarity). Note=Translocates to the cell
    membrane following binding to PtdIns(4,5)P2 (By similarity)

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane IEA--

    Find genes that share ontologies with AMER3           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for AMER3
    Interactions:

        Search GeneGlobe Interaction Network for AMER3

    4 Interacting proteins for AMER3 (Q8N9441) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    AMER1Q5JTC61EBI-8869590,EBI-6169747
    APCP250541EBI-8869590,EBI-727707
    AXIN2Q9Y2T11EBI-8869590,EBI-4400025
    Axin2O885661EBI-8869590,EBI-7690990
    About this table

    Gene Ontology (GO): 1 biological process term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016055Wnt signaling pathway IEA--

    Find genes that share ontologies with AMER3           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for AMER3



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for AMER3 gene (4 alternative transcripts): 
    NM_001105193.1  NM_001105194.1  NM_001105195.1  NM_152698.2  

    Unigene Cluster for AMER3:

    APC membrane recruitment protein 3
    Hs.369289  [show with all ESTs]
    Unigene Representative Sequence: NM_152698
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000321420(uc010fmv.2) ENST00000431758 ENST00000458606 ENST00000423981(uc002trw.2 uc010fms.1 uc010fmt.1 uc010fmu.1)

    miRNA
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    Predesigned siRNA for gene silencing in human, mouse, rat AMER3
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    Primer
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    Additional mRNA sequence: 

    AK095696.1 AK289525.1 AK291424.1 BC025667.1 BC113445.1 BC113447.1 BC143806.1 

    5 DOTS entries:

    DT.434326  DT.433963  DT.40296171  DT.120981030  DT.120981020 

    14 AceView cDNA sequences:

    BM931623 NM_152698 AI347882 AK095696 Z38230 AW270167 AA331856 Z41937 
    AI279217 T08731 AW339379 BM931771 H16063 H16064 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for AMER3    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b · 3c · 3d
    SP1:              -     -     -                           
    SP2:                    -     -                           
    SP3:                                                      
    SP4:                                                      


    ECgene alternative splicing isoforms for AMER3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    See probesets specificity/sensitivity at GeneAnnot
    CGAP TAG: --

    AMER3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    AMER3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.369289
        Custom PCR Arrays for AMER3
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AMER3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for AMER3 gene from Selected species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Amer31 , 5 family with sequence similarity 123, member C5
    APC membrane recruitment 31
    75.01(n)1
    67.1(a)1
      1 (13.34 cM)5
    2113831  NM_213727.21  NP_998892.11 
     345796935 
    chicken
    (Gallus gallus)
    Aves AMER36
    APC membrane recruitment protein 3
    29(a)
    1 ↔ 1
    9(2036682-2039627) ENSGALG00000026075
    lizard
    (Anolis carolinensis)
    Reptilia AMER36
    APC membrane recruitment protein 3
    49(a)
    1 ↔ 1
    3(37770690-37772333)
    zebrafish
    (Danio rerio)
    Actinopterygii AMER36
    APC membrane recruitment protein 3
    20(a)
    1 ↔ 1
    2(16186351-16200866) ENSDARG00000095819


    ENSEMBL Gene Tree for AMER3 (if available)
    TreeFam Gene Tree for AMER3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for AMER3 gene
    AMER22  AMER12  

    Find genes that share paralogs with AMER3           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for AMER3 (see all 401)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1901604171,2
    --131511128(+) CGGCCC/GGGCGT 1 -- us2k10--------
    rs1931645731,2
    --131511280(+) ATGCCC/TGTAAT 2 -- us2k10--------
    rs1440956611,2
    --131511388(+) AAGAAG/TAGTGA 2 -- us2k10--------
    rs75989071,2
    C,F,A,H--131511470(+) TGaaaT/Gaaaaa 2 -- us2k1 tfbs39Minor allele frequency- G:0.15NS EA NA WA 1208
    rs1399777711,2
    C--131511580(+) ATGGCC/TGACTC 2 -- us2k10--------
    rs1843576871,2
    C--131511581(+) TGGCCA/GACTCA 2 -- us2k10--------
    rs770436931,2
    C,F--131511653(+) TCTGAG/AACCCT 2 -- us2k11Minor allele frequency- A:0.18WA 118
    rs1887681131,2
    --131511832(+) GTGGCA/GCAATC 2 -- us2k10--------
    rs1811438981,2
    --131511938(+) GCTAAC/TTTTTT 4 -- us2k10--------
    rs1162107311,2
    C,F--131512019(+) CGCCCG/ATCTGG 4 -- us2k11Minor allele frequency- A:0.17WA 118

    HapMap Linkage Disequilibrium report for AMER3 (131513008 - 131525707 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for AMER3: --
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing AMER3
    DNA2.0 Custom Variant and Variant Library Synthesis for AMER3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for AMER3 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with AMER3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The WTX/AMER1 gene family: evolution, signature and function. (PubMed id 20843316)1, 3 Boutet A.... Schedl A. (BMC Evol. Biol. 2010)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    3. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
    4. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W.... Wilson R.K. (Nature 2005)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 205147 HGNC: 26771 AceView: FLJ38377 Ensembl:ENSG00000178171 euGenes: HUgn205147
    ECgene: AMER3 H-InvDB: AMER3

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for AMER3 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for AMER3 gene:
    Search GeneIP for patents involving AMER3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from genOway)
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    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

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