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ALOX5AP Gene(Protein Coding)

Arachidonate 5-Lipoxygenase Activating Protein

GCID:
GC13P030713
GIFtS:
63
Genes Participants
UDN Logo

Aliases for ALOX5AP Gene

Aliases for ALOX5AP Gene

  • Arachidonate 5-Lipoxygenase Activating Protein 2 3
  • MK-886-Binding Protein 2 3 4
  • Arachidonate 5-Lipoxygenase-Activating Protein 2 5
  • FLAP 3 4
  • Five-Lipoxygenase Activating Protein 2

External Ids for ALOX5AP Gene

Previous GeneCards Identifiers for ALOX5AP Gene

  • GC13P029289
  • GC13P025289
  • GC13P030239
  • GC13P029108
  • GC13P029109
  • GC13P030207
  • GC13P031309
  • GC13P012127

Summaries for ALOX5AP Gene

Entrez Gene Summary for ALOX5AP Gene

  • This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

GeneCards Summary for ALOX5AP Gene

ALOX5AP (Arachidonate 5-Lipoxygenase Activating Protein) is a Protein Coding gene. Diseases associated with ALOX5AP include stroke, ischemic and gastroesophageal junction adenocarcinoma. Among its related pathways are Leukotriene modifiers pathway, Pharmacodynamics and Arachidonic acid metabolism. GO annotations related to this gene include protein homodimerization activity and enzyme binding. An important paralog of this gene is MGST2.

UniProtKB/Swiss-Prot for ALOX5AP Gene

  • Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Tocris Summary for ALOX5AP Gene

  • Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1, 15-LOX-2 and E-LOX.

Gene Wiki entry for ALOX5AP Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ALOX5AP Gene

Genomics for ALOX5AP Gene

Products:

  1. Regulatory Element

Regulatory Elements for ALOX5AP Gene

Enhancers for ALOX5AP Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around ALOX5AP on UCSC Golden Path with GeneCards custom track

Promoters for ALOX5AP Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around ALOX5AP on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the ALOX5AP gene promoter:

Genomic Location for ALOX5AP Gene

Chromosome:
13
Start:
30,713,478 bp from pter
End:
30,764,428 bp from pter
Size:
50,951 bases
Orientation:
Plus strand

Genomic View for ALOX5AP Gene

Genes around ALOX5AP on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ALOX5AP Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ALOX5AP Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for ALOX5AP Gene

Proteins for ALOX5AP Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for ALOX5AP Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P20292-AL5AP_HUMAN
    Recommended name:
    Arachidonate 5-lipoxygenase-activating protein
    Protein Accession:
    P20292
    Secondary Accessions:
    • Q5VV04

    Protein attributes for ALOX5AP Gene

    Size:
    161 amino acids
    Molecular mass:
    18157 Da
    Quaternary structure:
    • Homotrimer. Interacts with LTC4S and ALOX5.

    Three dimensional structures from OCA and Proteopedia for ALOX5AP Gene

neXtProt entry for ALOX5AP Gene

Proteomics data for ALOX5AP Gene at MOPED

Protein Expression for ALOX5AP Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for ALOX5AP Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ALOX5AP Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for ALOX5AP Gene

Domains & Families for ALOX5AP Gene

Protein Domains for ALOX5AP Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for ALOX5AP Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P20292

UniProtKB/Swiss-Prot:

AL5AP_HUMAN :
  • The C-terminal part after residue 140 is mostly unstructured.
  • Belongs to the MAPEG family.
Domain:
  • The C-terminal part after residue 140 is mostly unstructured.
Family:
  • Belongs to the MAPEG family.
genes like me logo Genes that share domains with ALOX5AP: view

No data available for Gene Families for ALOX5AP Gene

Function for ALOX5AP Gene

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  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line
  7. Flow Cytometry

Molecular function for ALOX5AP Gene

GENATLAS Biochemistry:
arachidonate 5-lipoxygenase activating protein,expressed in cells of myeloid origin,localized in the nuclear envelop,involved in the biosynthesis of leukotrienes (role in inflammation),microsomal,GST superfamily
UniProtKB/Swiss-Prot Function:
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Gene Ontology (GO) - Molecular Function for ALOX5AP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004364 NOT glutathione transferase activity IBA --
GO:0005515 protein binding IEA,IPI 19233132
GO:0047485 protein N-terminus binding IPI 19233132
GO:0050544 arachidonic acid binding IDA 8440384
genes like me logo Genes that share ontologies with ALOX5AP: view

Phenotypes for ALOX5AP Gene

MGI mutant phenotypes for ALOX5AP:
inferred from 2 alleles
GenomeRNAi human phenotypes for ALOX5AP:
genes like me logo Genes that share phenotypes with ALOX5AP: view

Animal Models for ALOX5AP Gene

MGI Knock Outs for ALOX5AP:

Animal Model Products

CRISPR Products

miRNA for ALOX5AP Gene

miRTarBase miRNAs that target ALOX5AP

Inhibitory RNA Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ALOX5AP Gene

Localization for ALOX5AP Gene

Subcellular locations from UniProtKB/Swiss-Prot for ALOX5AP Gene

Nucleus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ALOX5AP Gene COMPARTMENTS Subcellular localization image for ALOX5AP gene
Compartment Confidence
endoplasmic reticulum 5
nucleus 5
cytosol 2
lysosome 2
plasma membrane 2
vacuole 2

Gene Ontology (GO) - Cellular Components for ALOX5AP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol IEA --
genes like me logo Genes that share ontologies with ALOX5AP: view

Pathways & Interactions for ALOX5AP Gene

genes like me logo Genes that share pathways with ALOX5AP: view

Gene Ontology (GO) - Biological Process for ALOX5AP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0044281 small molecule metabolic process TAS --
genes like me logo Genes that share ontologies with ALOX5AP: view

No data available for SIGNOR curated interactions for ALOX5AP Gene

Drugs & Compounds for ALOX5AP Gene

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  1. Drug

(20) Drugs for ALOX5AP Gene - From: DrugBank, ApexBio, DGIdb, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Zileuton Approved, Investigational, Withdrawn Pharma Inhibition, Inhibitor 5-lipoxygenase (5-LOX) inhibitor, orally active 20
DG031 Investigational Pharma inhibitor, Target 0
AM103 Investigational Pharma Target 0
Malotilate Pharma Tyrosine kinase inhibitors 0
AM-103 Pharma 0

(20) Additional Compounds for ALOX5AP Gene - From: Novoseek and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
MK 886
118414-82-7
2-TEDC
132465-10-2
BW-B 70C
134470-38-5
STEARDA
105955-10-0

(5) Tocris Compounds for ALOX5AP Gene

Compound Action Cas Number
2-TEDC 5-, 12-, 15-Lipoxygenase inhibitor 132465-10-2
Baicalein 5- and 12-Lipoxygenase inhibitor 491-67-8
BW-B 70C 5-Lipoxygenase inhibitor 134470-38-5
MK 886 Inhibitor of 5-lipoxygenase-activating protein (FLAP) 118414-82-7
STEARDA Endogenous lipid, active in vivo. Enhances effects of endovanilloids at TRPV1 receptors 105955-10-0

(2) ApexBio Compounds for ALOX5AP Gene

Compound Action Cas Number
Malotilate Tyrosine kinase inhibitors 59937-28-9
Zileuton 5-lipoxygenase (5-LOX) inhibitor, orally active 111406-87-2
genes like me logo Genes that share compounds with ALOX5AP: view

Drug Products

Transcripts for ALOX5AP Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
  5. Flow Cytometry

mRNA/cDNA for ALOX5AP Gene

(4) REFSEQ mRNAs :
(2) Additional mRNA sequences :
(167) Selected AceView cDNA sequences:
(3) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for ALOX5AP Gene

Arachidonate 5-lipoxygenase-activating protein:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ALOX5AP Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b
SP1: - - -
SP2: -
SP3: - - - - -
SP4: -

Relevant External Links for ALOX5AP Gene

GeneLoc Exon Structure for
ALOX5AP
ECgene alternative splicing isoforms for
ALOX5AP

Expression for ALOX5AP Gene

Products:

  1. Primer

mRNA expression in normal human tissues for ALOX5AP Gene

mRNA expression in normal human tissues for ALOX5AP Gene
mRNA expression by BioGPS for ALOX5AP GenemRNA expression by GTEx for ALOX5AP Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ALOX5AP Gene

This gene is overexpressed in Whole Blood (x40.3).

Protein differential expression in normal tissues from HIPED for ALOX5AP Gene

This gene is overexpressed in Nasal epithelium (37.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for ALOX5AP Gene



SOURCE GeneReport for Unigene cluster for ALOX5AP Gene Hs.507658

genes like me logo Genes that share expression patterns with ALOX5AP: view

Protein tissue co-expression partners for ALOX5AP Gene

- Elite partner

Primer Products

No data available for mRNA Expression by UniProt/SwissProt for ALOX5AP Gene

Orthologs for ALOX5AP Gene

This gene was present in the common ancestor of chordates.

Orthologs for ALOX5AP Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
 Mammalia ALOX5AP 35
  • 86.13 (n)
  • 90.68 (a)
ALOX5AP 36
  • 91 (a)
 OneToOne
dog
(Canis familiaris)
 Mammalia ALOX5AP 35
  • 86.89 (n)
  • 91.26 (a)
ALOX5AP 36
  • 78 (a)
 OneToOne
mouse
(Mus musculus)
 Mammalia Alox5ap 35
  • 85.71 (n)
  • 91.93 (a)
Alox5ap 16
Alox5ap 36
  • 92 (a)
 OneToOne
chimpanzee
(Pan troglodytes)
 Mammalia ALOX5AP 35
  • 98.62 (n)
  • 99.08 (a)
ALOX5AP 36
  • 100 (a)
 OneToOne
rat
(Rattus norvegicus)
 Mammalia Alox5ap 35
  • 85.09 (n)
  • 91.93 (a)
oppossum
(Monodelphis domestica)
 Mammalia ALOX5AP 36
  • 91 (a)
 OneToOne
platypus
(Ornithorhynchus anatinus)
 Mammalia ALOX5AP 36
  • 90 (a)
 OneToOne
chicken
(Gallus gallus)
 Aves ALOX5AP 35
  • 76.19 (n)
  • 82.61 (a)
ALOX5AP 36
  • 82 (a)
 OneToOne
lizard
(Anolis carolinensis)
 Reptilia ALOX5AP 36
  • 72 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia alox5ap 35
  • 72.05 (n)
  • 79.5 (a)
Str.11819 35
zebrafish
(Danio rerio)
 Actinopterygii alox5ap 35
  • 62.82 (n)
  • 58.97 (a)
wufj65d02 35
alox5ap 36
  • 57 (a)
 OneToOne
Species with no ortholog for ALOX5AP:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for ALOX5AP Gene

ENSEMBL:
Gene Tree for ALOX5AP (if available)
TreeFam:
Gene Tree for ALOX5AP (if available)

Paralogs for ALOX5AP Gene

Paralogs for ALOX5AP Gene

(2) SIMAP similar genes for ALOX5AP Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with ALOX5AP: view

Variants for ALOX5AP Gene

Sequence variations from dbSNP and Humsavar for ALOX5AP Gene

SNP ID Clin Chr 13 pos Sequence Context AA Info Type
rs1132340 -- 30,764,325(+) TTCCC(A/G)TGCAT utr-variant-3-prime
rs3803277 -- 30,744,171(+) CCTAA(A/C)CCTGA intron-variant
rs3803278 -- 30,744,264(+) CCAAG(C/T)TTCTG intron-variant
rs3809376 -- 30,734,585(+) CCCAC(A/C)CAGCA intron-variant, upstream-variant-2KB
rs3885907 -- 30,740,318(+) CCCTC(A/C)GTTTC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ALOX5AP Gene

Variant ID Type Subtype PubMed ID
nsv832574 CNV Gain 17160897
esv2747225 CNV Deletion 23290073
nsv984 CNV Insertion 18451855

Variation tolerance for ALOX5AP Gene

Residual Variation Intolerance Score: 47.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.47; 10.28% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ALOX5AP Gene

HapMap Linkage Disequilibrium report
ALOX5AP
Human Gene Mutation Database (HGMD)
ALOX5AP

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ALOX5AP Gene

Disorders for ALOX5AP Gene

MalaCards: The human disease database

(5) MalaCards diseases for ALOX5AP Gene - From: OMIM, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
stroke, ischemic
  • ischemic stroke
gastroesophageal junction adenocarcinoma
  • adenocarcinoma of cardioesophageal junction
asthma
  • asthma, protection against
myocardial infarction
  • myocardial infarction, protection against
cerebrovascular disease
  • stroke
- elite association - COSMIC cancer census association via MalaCards

UniProtKB/Swiss-Prot

AL5AP_HUMAN
  • Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. {ECO:0000269 PubMed:14770184, ECO:0000269 PubMed:17304054}.

Relevant External Links for ALOX5AP

Genetic Association Database (GAD)
ALOX5AP
Human Genome Epidemiology (HuGE) Navigator
ALOX5AP
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
ALOX5AP
genes like me logo Genes that share disorders with ALOX5AP: view

No data available for Genatlas for ALOX5AP Gene

Publications for ALOX5AP Gene

  1. Gene characterization and promoter analysis of the human 5- lipoxygenase-activating protein (FLAP). (PMID: 1673682) Kennedy B.P. … Dixon R.A.F. (J. Biol. Chem. 1991) 2 3 4 23 67
  2. Cytogenetic and radiation hybrid mapping of human arachidonate 5-lipoxygenase-activating protein (ALOX5AP) to chromosome 13q12. (PMID: 10036194) Yandava C.N. … Drazen J.M. (Genomics 1999) 2 3 23
  3. The role of LTA4H and ALOX5AP genes in the risk for asthma in Latinos. (PMID: 20067482) Via M. … GonzA!lez Burchard E. (Clin. Exp. Allergy 2010) 3 23
  4. Hypoxia-mediated expression of 5-lipoxygenase-activating protein involves HIF-1alpha and NF-kappaB and microRNAs 135a and 199a-5p. (PMID: 20194722) Gonsalves C.S. … Kalra V.K. (J. Immunol. 2010) 3 23
  5. Association of a genetic variant in the ALOX5AP with higher risk of ischemic stroke: a case-control, meta-analysis and functional study. (PMID: 20357438) Domingues-Montanari S. … Montaner J. (Cerebrovasc. Dis. 2010) 3 23

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