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Aliases for ALKBH8 Gene

Aliases for ALKBH8 Gene

  • AlkB Homolog 8, TRNA Methyltransferase 2 3
  • S-Adenosyl-L-Methionine-Dependent TRNA Methyltransferase ABH8 3 4
  • TRNA (Carboxymethyluridine(34)-5-O)-Methyltransferase ABH8 3 4
  • Probable Alpha-Ketoglutarate-Dependent Dioxygenase ABH8 3 4
  • ABH8 3 4
  • TRNA Methyltransferase 9 Homolog (S. Cerevisiae) 2
  • AlkB, Alkylation Repair Homolog 8 (E. Coli) 2
  • AlkB, Alkylation Repair Homolog 8 3
  • TRNA Methyltransferase 9 Homolog 3
  • AlkB Homologue 8 3
  • EC 1.14.11.- 4
  • EC 2.1.1.229 4
  • TRMT9 3
  • TRM9 3

External Ids for ALKBH8 Gene

Previous GeneCards Identifiers for ALKBH8 Gene

  • GC11M106879
  • GC11M107373
  • GC11M103298

Summaries for ALKBH8 Gene

GeneCards Summary for ALKBH8 Gene

ALKBH8 (AlkB Homolog 8, TRNA Methyltransferase) is a Protein Coding gene. Among its related pathways are Gene Expression and tRNA modification in the nucleus and cytosol. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is KIAA1456.

UniProtKB/Swiss-Prot for ALKBH8 Gene

  • Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ALKBH8 Gene

Genomics for ALKBH8 Gene

Regulatory Elements for ALKBH8 Gene

Genomic Location for ALKBH8 Gene

Chromosome:
11
Start:
107,502,726 bp from pter
End:
107,565,746 bp from pter
Size:
63,021 bases
Orientation:
Minus strand

Genomic View for ALKBH8 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ALKBH8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ALKBH8 Gene

Proteins for ALKBH8 Gene

  • Protein details for ALKBH8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96BT7-ALKB8_HUMAN
    Recommended name:
    Alkylated DNA repair protein alkB homolog 8
    Protein Accession:
    Q96BT7
    Secondary Accessions:
    • B1Q2M0
    • B4DEF6
    • Q8N989

    Protein attributes for ALKBH8 Gene

    Size:
    664 amino acids
    Molecular mass:
    75208 Da
    Cofactor:
    Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.;
    Quaternary structure:
    • Interacts with TRMT112.

    Three dimensional structures from OCA and Proteopedia for ALKBH8 Gene

    Alternative splice isoforms for ALKBH8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ALKBH8 Gene

Proteomics data for ALKBH8 Gene at MOPED

Post-translational modifications for ALKBH8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ALKBH8 Gene

Antibody Products

  • Santa Cruz Biotechnology (SCBT) Antibodies for ALKBH8

No data available for DME Specific Peptides for ALKBH8 Gene

Domains & Families for ALKBH8 Gene

Suggested Antigen Peptide Sequences for ALKBH8 Gene

Graphical View of Domain Structure for InterPro Entry

Q96BT7

UniProtKB/Swiss-Prot:

ALKB8_HUMAN :
  • Contains 1 Fe2OG dioxygenase domain.
  • Belongs to the alkB family.
Domain:
  • Contains 1 Fe2OG dioxygenase domain.
  • Contains 1 RRM (RNA recognition motif) domain.
Family:
  • Belongs to the alkB family.
genes like me logo Genes that share domains with ALKBH8: view

Function for ALKBH8 Gene

Molecular function for ALKBH8 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
S-adenosyl-L-methionine + carboxymethyluridine(34) in tRNA = S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA.
UniProtKB/Swiss-Prot Function:
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).
UniProtKB/Swiss-Prot Induction:
Up-regulated after DNA damage. Induction is mediated via ATM.

Enzyme Numbers (IUBMB) for ALKBH8 Gene

Gene Ontology (GO) - Molecular Function for ALKBH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000049 tRNA binding IDA 22065580
GO:0000166 nucleotide binding --
GO:0003676 nucleic acid binding --
GO:0005506 iron ion binding ISS --
GO:0005515 protein binding IPI 20123966
genes like me logo Genes that share ontologies with ALKBH8: view
genes like me logo Genes that share phenotypes with ALKBH8: view

Animal Models for ALKBH8 Gene

MGI Knock Outs for ALKBH8:

Animal Model Products

No data available for miRNA , Transcription Factor Targets and HOMER Transcription for ALKBH8 Gene

Localization for ALKBH8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ALKBH8 Gene

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. {ECO:0000269 PubMed:20308323}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ALKBH8 Gene COMPARTMENTS Subcellular localization image for ALKBH8 gene
Compartment Confidence
cytosol 5
nucleus 5
cytoskeleton 4

Gene Ontology (GO) - Cellular Components for ALKBH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 20308323
GO:0005654 nucleoplasm IDA --
GO:0005829 cytosol IDA 20308323
GO:0015630 microtubule cytoskeleton IDA --
genes like me logo Genes that share ontologies with ALKBH8: view

Pathways & Interactions for ALKBH8 Gene

genes like me logo Genes that share pathways with ALKBH8: view

Pathways by source for ALKBH8 Gene

Gene Ontology (GO) - Biological Process for ALKBH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002098 tRNA wobble uridine modification IDA 21285950
GO:0006400 tRNA modification TAS --
GO:0006974 cellular response to DNA damage stimulus IDA 20308323
GO:0008033 tRNA processing TAS --
GO:0008152 metabolic process --
genes like me logo Genes that share ontologies with ALKBH8: view

No data available for SIGNOR curated interactions for ALKBH8 Gene

Drugs & Compounds for ALKBH8 Gene

(2) Drugs for ALKBH8 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
S-Adenosylmethionine Approved Nutra 0
s-adenosylhomocysteine Experimental Pharma 0
genes like me logo Genes that share compounds with ALKBH8: view

Transcripts for ALKBH8 Gene

Unigene Clusters for ALKBH8 Gene

AlkB, alkylation repair homolog 8 (E. coli):
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ALKBH8

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ALKBH8 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b
SP1: - -
SP2:
SP3:
SP4: -

Relevant External Links for ALKBH8 Gene

GeneLoc Exon Structure for
ALKBH8
ECgene alternative splicing isoforms for
ALKBH8

Expression for ALKBH8 Gene

mRNA expression in normal human tissues for ALKBH8 Gene

Protein differential expression in normal tissues from HIPED for ALKBH8 Gene

This gene is overexpressed in CD4 Tcells (48.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for ALKBH8 Gene



SOURCE GeneReport for Unigene cluster for ALKBH8 Gene Hs.503763

mRNA Expression by UniProt/SwissProt for ALKBH8 Gene

Q96BT7-ALKB8_HUMAN
Tissue specificity: Widely expressed, with highest expression in spleen, followed by pancreas and lung.
genes like me logo Genes that share expression patterns with ALKBH8: view

Protein tissue co-expression partners for ALKBH8 Gene

Primer Products

  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for ALKBH8 Gene

Orthologs for ALKBH8 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ALKBH8 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia ALKBH8 35
  • 89.16 (n)
  • 85.54 (a)
ALKBH8 36
  • 86 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ALKBH8 35
  • 85.73 (n)
  • 83.06 (a)
ALKBH8 36
  • 81 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Alkbh8 35
  • 81.07 (n)
  • 77.26 (a)
Alkbh8 16
Alkbh8 36
  • 77 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia ALKBH8 35
  • 99.15 (n)
  • 98.34 (a)
ALKBH8 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Alkbh8 35
  • 80.57 (n)
  • 76.81 (a)
oppossum
(Monodelphis domestica)
Mammalia ALKBH8 36
  • 73 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ALKBH8 36
  • 71 (a)
OneToOne
chicken
(Gallus gallus)
Aves ALKBH8 35
  • 72.43 (n)
  • 68.13 (a)
ALKBH8 36
  • 62 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia alkbh8 35
  • 66.94 (n)
  • 66.18 (a)
zebrafish
(Danio rerio)
Actinopterygii alkbh8 35
  • 60.15 (n)
  • 61.73 (a)
Dr.17847 35
alkbh8 36
  • 56 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP011900 35
  • 50.33 (n)
  • 48.16 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG17807 35
  • 53.1 (n)
  • 50.09 (a)
CG17807 36
  • 44 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea alkb-8 35
  • 47.79 (n)
  • 40.53 (a)
alkb-8 36
  • 37 (a)
ManyToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADR181W 35
  • 50.85 (n)
  • 43.92 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B01023g 35
  • 49.37 (n)
  • 40.23 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TRM9 35
  • 47.79 (n)
  • 39.34 (a)
TRM9 36
  • 37 (a)
OneToMany
bread mold
(Neurospora crassa)
Ascomycetes NCU01705 35
  • 44.22 (n)
  • 34.66 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes trm9 35
  • 56.04 (n)
  • 51.69 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6013 36
  • 41 (a)
OneToOne
Species with no ortholog for ALKBH8:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ALKBH8 Gene

ENSEMBL:
Gene Tree for ALKBH8 (if available)
TreeFam:
Gene Tree for ALKBH8 (if available)

Paralogs for ALKBH8 Gene

Paralogs for ALKBH8 Gene

Pseudogenes.org Pseudogenes for ALKBH8 Gene

genes like me logo Genes that share paralogs with ALKBH8: view

Variants for ALKBH8 Gene

Sequence variations from dbSNP and Humsavar for ALKBH8 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs56163485 -- 107,551,164(+) TTATT(G/T)GGTTT intron-variant
rs55928933 -- 107,506,425(+) GACCA(C/T)AGAAA intron-variant
rs55846094 -- 107,566,821(+) AGTAG(A/T)GGCAG upstream-variant-2KB
rs55784935 -- 107,518,081(+) TAAAA(G/T)ACAGA intron-variant
rs55658681 -- 107,511,455(+) TTACC(A/G)TATAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ALKBH8 Gene

Variant ID Type Subtype PubMed ID
dgv1312n71 CNV Gain 21882294
nsv898373 CNV Gain 21882294
nsv898376 CNV Gain 21882294

Variation tolerance for ALKBH8 Gene

Gene Damage Index Score: 4.83; 67.12% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ALKBH8 Gene

HapMap Linkage Disequilibrium report
ALKBH8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ALKBH8 Gene

Disorders for ALKBH8 Gene

Relevant External Links for ALKBH8

Genetic Association Database (GAD)
ALKBH8
Human Genome Epidemiology (HuGE) Navigator
ALKBH8
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
ALKBH8
genes like me logo Genes that share disorders with ALKBH8: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ALKBH8 Gene

Publications for ALKBH8 Gene

  1. ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA. (PMID: 21285950) van den Born E. … Falnes P.A.9. (Nat Commun 2011) 2 67
  2. Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding. (PMID: 20123966) Songe-MA … Klungland A. (Mol. Cell. Biol. 2010) 2 67
  3. A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression. (PMID: 19293182) Shimada K. … Konishi N. (Cancer Res. 2009) 23 67
  4. Shared genetic susceptibility to ischemic stroke and coronary artery disease: a genome-wide analysis of common variants. (PMID: 24262325) Dichgans M. … . (Stroke 2014) 67
  5. Crystal structure and RNA binding properties of the RNA recognition motif (RRM) and AlkB domains in human AlkB homolog 8 (ABH8), an enzyme catalyzing tRNA hypermodification. (PMID: 22065580) Pastore C. … Hunt J.F. (J. Biol. Chem. 2012) 67

Products for ALKBH8 Gene

Sources for ALKBH8 Gene

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