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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ALG14 Gene

protein-coding   GIFtS: 54
GCID: GC01M095449

ALG14, UDP-N-acetylglucosaminyltransferase subunit

(Previous names: asparagine-linked glycosylation 14 homolog (yeast), asparagine-linked...)
 Explore 1 disease affiliated with
ALG14 via our new
 Human Malady Compendium 
Biological research products
for ALG14
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
ALG14, UDP-N-Acetylglucosaminyltransferase Subunit1 2
MGC197801
Asparagine-Linked Glycosylation 14 Homolog (S. Cerevisiae)1
Asparagine-Linked Glycosylation 14 Homolog (Yeast)1
UDP-N-Acetylglucosamine Transferase Subunit ALG14 Homolog2

External Ids:    HGNC: 282871   Entrez Gene: 1998572   Ensembl: ENSG000001723397   OMIM: 6128665   UniProtKB: Q96F253   

Export aliases for ALG14 gene to outside databases

Previous GC identifers: GC01M095161 GC01M093570


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ALG14:
Asparagine (N)-glycosylation is an essential modification that regulates protein folding and stability. ALG13 and ALG14
(MIM 612866) constitute the UDP-GlcNAc transferase, which catalyzes a key step in endoplasmic reticulum N-linked
glycosylation (Averbeck et al., 2007 (PubMed 17686769)).(supplied by OMIM, Jun 2009)

UniProtKB/Swiss-Prot: ALG14_HUMAN, Q96F25
Function: May be involved in protein N-glycosylation. May play a role in the second step of the dolichol-linked
oligosaccharide pathway. May anchor the catalytic subunit ALG13 to the ER

Gene Wiki entry for ALG14


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_032977.9  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ALG14 gene promoter:
         HOXA9B   HOXA9   p300   Nkx2-5   HNF-3beta   Evi-1   Meis-1b   FOXO4   LyF-1   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidALG14 promoter sequence
   Search SABiosciences Chromatin IP Primers for ALG14

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ALG14


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p21.3   Ensembl cytogenetic band:  1p21.3   HGNC cytogenetic band: 1p21.3

ALG14 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ALG14 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M095449:  view genomic region     (about GC identifiers)

Start:
95,448,279 bp from pter      End:
95,538,507 bp from pter
Size:
90,229 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ALG14_HUMAN, Q96F25 (See protein sequence)
Recommended Name: UDP-N-acetylglucosamine transferase subunit ALG14 homolog  
Size: 216 amino acids; 24151 Da
Subunit: Heterodimer with ALG13 isoform 2 to form a functional enzyme (By similarity)
Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein (Probable)
Secondary accessions: A8K030

Explore the universe of human proteins at neXtProt for ALG14: NX_Q96F25

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96F25

  • ALG14 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_659425.1  
    ENSEMBL proteins: 
     ENSP00000359224  
    Reactome Protein details: Q96F25
    Human Recombinant Protein Products: 
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    Uscn Proteins for ALG14

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005789endoplasmic reticulum membrane TAS--
    GO:0016021integral to membrane IEA--


    ALG14 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ALG14 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR013969 Oligosacch_biosynth_Alg14

    Graphical View of Domain Structure for InterPro Entry Q96F25

    ProtoNet protein and cluster: Q96F25

    1 Blocks protein family: IPB013969 Oligosaccharide biosynthesis protein Alg14 like

    UniProtKB/Swiss-Prot: ALG14_HUMAN, Q96F25
    Similarity: Belongs to the ALG14 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ALG14_HUMAN, Q96F25
    Function: May be involved in protein N-glycosylation. May play a role in the second step of the dolichol-linked
    oligosaccharide pathway. May anchor the catalytic subunit ALG13 to the ER

    miRNA
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    SwitchGear 3'UTR luciferase reporter plasmidALG14 3' UTR sequence
    Inhib. RNA
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ALG14

    1 GenomeRNAi human phenotype for ALG14:
     Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Asparagine N-linked glycosylation
    Asparagine N-linked glycosylation1.00
    Post-translational protein modification0.44
    N-Glycan biosynthesis0.52
    Metabolism of proteins0.15
    2Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
    Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein1.00
    3Metabolism
    Metabolic pathways0.38

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    4        Reactome Pathways for ALG14
        Asparagine N-linked glycosylation
    Metabolism of proteins
    Post-translational protein modification
    Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein


    2         Kegg Pathways  (Kegg details for ALG14):
        N-Glycan biosynthesis
    Metabolic pathways


    ALG14 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ALG14

    STRING Interaction Network Preview (showing 5 interactants - click image to see 6)

    5/6 Interacting proteins for ALG14 (ENSP000003592244) via UniProtKB, MINT, STRING, and/or I2D (see all 6)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ALG1ENSP000002623744STRING: ENSP00000262374
    DPAGT1ENSP000003461424STRING: ENSP00000346142
    DPM1ENSP000003606444STRING: ENSP00000360644
    ALG13ENSP000003610474STRING: ENSP00000361047
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006488dolichol-linked oligosaccharide biosynthetic process TAS--
    GO:0018279protein N-linked glycosylation via asparagine TAS--
    GO:0043687post-translational protein modification TAS--
    GO:0044267cellular protein metabolic process TAS--


    ALG14 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ALG14

    4 HMDB Compounds for ALG14    About this table
    CompoundSynonyms CAS #PubMed Ids
    N,N'-DiacetylchitobiosyldiphosphodolicholChitobiosyldiphosphodolichol (see all 2)----
    N-Acetyl-D-glucosaminyldiphosphodolicholN-Acetyl-D-glucosaminyl-diphosphodolichol ----
    Uridine 5'-diphosphate5'-UDP (see all 9)58-98-0--
    Uridine diphosphate-N-acetylglucosamineN-[2-[[[5-[(2,4-dioxo-1H-pyrimidin-1-yl)]-3,4-dihydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxy-4,5-dihydroxy-6-(hydroxymethyl)tetrahydropyran-3-yl]acetamide (see all 30)528-04-1--
    Search CenterWatch for drugs/clinical trials and news about ALG14 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ALG14 gene: 
    NM_144988.3  

    Unigene Cluster for ALG14:

    Asparagine-linked glycosylation 14 homolog (S. cerevisiae)
    Hs.408927  [show with all ESTs]
    Unigene Representative Sequence: AK289395
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000370205(uc001dra.2) ENST00000495856

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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ALG14

    Additional cDNA sequence: 

    AK289395.1 BC011706.2 

    13 DOTS entries:

    DT.307367  DT.75102999  DT.121434274  DT.100793183  DT.91762397  DT.100793184  DT.95275478  DT.91762400 
    DT.91762404  DT.95175685  DT.121434255  DT.91762422  DT.91762428 

    24/173 AceView cDNA sequences (see all 173):

    BG824624 BG189903 BG185117 BG187579 BU682208 BM923794 BC011706 NM_144988 
    BE392296 BG209538 BG830360 BG204242 BG422099 AA505678 BG205288 BG191928 
    BG200132 BE729382 BG199074 BG750416 BG196054 BG203220 BX474159 BG185118 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for ALG14    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b
    SP1:                          -                                 
    SP2:                          -     -     -                     
    SP3:                                                            


    ECgene alternative splicing isoforms for ALG14

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ALG14 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTTCTTGTAA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ALG14 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ALG14

    SOURCE GeneReport for Unigene cluster: Hs.408927
        SABiosciences Custom PCR Arrays for ALG14
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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for ALG14 gene from 9/29 species (see all 29)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Alg141 , 5 asparagine-linked glycosylation 14 homolog (yeast)5
    asparagine-linked glycosylation 141
    80.09(n)1
    76.39(a)1
      3 (52.94 cM)5
    667891  NM_024178.21  NP_077140.11 
     1212918175 
    chicken
    (Gallus gallus)
    Aves ALG141 asparagine-linked glycosylation 14 homolog (S. cerevisiae) 74.01(n)
    72.88(a)
      424484  XM_430156.3  XP_430156.2 
    lizard
    (Anolis carolinensis)
    Reptilia ALG146
    --
    61(a)
    1 ↔ 1
    4(89380852-89425357)
    African clawed frog
    (Xenopus laevis)
    Amphibia 480129352   -- 72.97(n)    48012935 
    zebrafish
    (Danio rerio)
    Actinopterygii CD605349.12   -- 75.87(n)    CD605349.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG63081 CG6308 48.21(n)
    47.62(a)
      32479  NM_132803.2  NP_573031.1 
    worm
    (Caenorhabditis elegans)
    Secernentea hpo-161 Protein HPO-16 50(n)
    40.79(a)
      187398  NM_068016.1  NP_500417.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT4G182301 beta-1,4-N-acetylglucosaminyltransferase 51.7(n)
    45.92(a)
      827549  NM_117933.5  NP_193557.2 
    rice
    (Oryza sativa)
    Liliopsida --
    UDP-N-acetylglucosamine transferase subunit ALG14,...
    34(a)
    1 ↔ 1
    3(17615013-17621125)


    ENSEMBL Gene Tree for ALG14 (if available)
    TreeFam Gene Tree for ALG14 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1421 NCBI SNPs in ALG14 are shown (see all 1421    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1406896571,2
    --95447893(+) GTCAGA/GCTGGT 1 -- ds50010--------
    rs66945351,2
    C,F,--95447933(+) CGCCTC/TAGCCT 1 -- ds50015Minor allele frequency- T:0.30NA CSA 10
    rs1846685431,2
    --95447964(+) GGTGTA/GAGCCG 1 -- ds50010--------
    rs1900353771,2
    --95447977(+) ATGCCA/GGCCTA 1 -- ds50010--------
    rs66821851,2
    C,H--95448196(+) TGTATC/TTGAAC 1 -- ds50014Minor allele frequency- T:0.00NS EA 420
    rs66946391,2
    H--95448254(+) ACTTTA/TCaaaa 1 -- ds50014Minor allele frequency- T:0.00NS EA 388
    rs1449533231,2
    --95448292(+) ACAGGA/CAATAT 1 -- ut310--------
    rs1490248841,2
    --95448400(+) AAAAGA/CAATTT 1 -- ut310--------
    rs1808125851,2
    --95448424(+) ATAAAC/TATTTA 1 -- ut310--------
    rs792062061,2
    --95448578(+) CCAATT/CTGAGT 1 -- ut311Minor allele frequency- C:0.01NA 120

    HapMap Linkage Disequilibrium report for ALG14 (95448279 - 95538507 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for ALG14
         2 CNVs: 4235 60859

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ALG14
    DNA2.0 Custom Variant and Variant Library Synthesis for ALG14

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ALG14 for disorders           About GeneDecksing

    OMIM gene information: 612866    OMIM disorders: --

    1 disease for ALG14:    About MalaCards
    malaria

    1 disease from the University of Copenhagen DISEASES database for ALG14:
    Congenital myasthenic syndrome

    Export disorders for ALG14 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ALG14 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with ALG14)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation. (PubMed id 16100110)1, 2 Gao X.-D.... Dean N. (2005)
    2. Two proteins homologous to the N- and C-terminal domains of the bacterial glycosyltransferase Murg are required for the second step of dolichyl-linked oligosaccharide synthesis in Saccharomyces cerevisiae. (PubMed id 15615718)1, 3 Chantret I....Moore S.E. (2005)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Genome-wide association study identifies novel loci as sociated with circulating phospho- and sphingolipid concentrations. (PubMed id 22359512)1 Demirkan A....Schmitz G. (2012)
    5. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    6. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    7. Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit. (PubMed id 17686769)1 Averbeck N....Dean N. (2007)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    10. Biosynthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Alg13p and Alg14p form a complex required for the formation of GlcNAc(2)-PP-dolichol. (PubMed id 16100113)9 Bickel T....Jakob C.A. (2005)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 199857 HGNC: 28287 AceView: MGC19780 Ensembl:ENSG00000172339 euGenes: HUgn199857
    ECgene: ALG14 Kegg: 199857 H-InvDB: ALG14

    (According to HUGE)
    About This Section
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    About This Section
    NameDescription
    PharmGKB entry for ALG14 Pharmacogenomics, SNPs, Pathways
    GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/

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