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Aliases for AKT1S1 Gene

Aliases for AKT1S1 Gene

  • AKT1 Substrate 1 (Proline-Rich) 2 3
  • 40 KDa Proline-Rich AKT Substrate 3 4
  • PRAS40 3 4
  • Proline-Rich AKT1 Substrate 1 3
  • Proline-Rich Akt Substrate 2
  • 40 KDa 2
  • Lobe 3

External Ids for AKT1S1 Gene

Previous GeneCards Identifiers for AKT1S1 Gene

  • GC19M055067
  • GC19M055071
  • GC19M055073
  • GC19M050372
  • GC19M046748

Summaries for AKT1S1 Gene

Entrez Gene Summary for AKT1S1 Gene

  • AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

GeneCards Summary for AKT1S1 Gene

AKT1S1 (AKT1 Substrate 1 (Proline-Rich)) is a Protein Coding gene. Among its related pathways are PI-3K cascade and PI-3K cascade.

UniProtKB/Swiss-Prot for AKT1S1 Gene

  • Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at Thr-389, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Gene Wiki entry for AKT1S1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AKT1S1 Gene

Genomics for AKT1S1 Gene

Regulatory Elements for AKT1S1 Gene

Transcription factor binding sites by QIAGEN in the AKT1S1 gene promoter:

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for AKT1S1 Gene

Start:
49,869,033 bp from pter
End:
49,878,459 bp from pter
Size:
9,427 bases
Orientation:
Minus strand

Genomic View for AKT1S1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for AKT1S1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AKT1S1 Gene

Proteins for AKT1S1 Gene

  • Protein details for AKT1S1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96B36-AKTS1_HUMAN
    Recommended name:
    Proline-rich AKT1 substrate 1
    Protein Accession:
    Q96B36
    Secondary Accessions:
    • A8MTQ1
    • B2RE93
    • J3KPM3
    • Q96BI4
    • Q96IK7
    • Q96NG2
    • Q9BWR5

    Protein attributes for AKT1S1 Gene

    Size:
    256 amino acids
    Molecular mass:
    27383 Da
    Quaternary structure:
    • Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins.
    SequenceCaution:
    • Sequence=AAH00031.2; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for AKT1S1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for AKT1S1 Gene

Proteomics data for AKT1S1 Gene at MOPED

Post-translational modifications for AKT1S1 Gene

  • Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for AKT1S1 (PRAS40)

Assay Products

  • antibodies-online kits for AKT1S1

No data available for DME Specific Peptides for AKT1S1 Gene

Domains for AKT1S1 Gene

Protein Domains for AKT1S1 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for AKT1S1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with AKT1S1: view

No data available for Gene Families and UniProtKB/Swiss-Prot for AKT1S1 Gene

Function for AKT1S1 Gene

Molecular function for AKT1S1 Gene

UniProtKB/Swiss-Prot Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at Thr-389, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Gene Ontology (GO) - Molecular Function for AKT1S1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 12524439
genes like me logo Genes that share ontologies with AKT1S1: view
genes like me logo Genes that share phenotypes with AKT1S1: view

Animal Model Products

CRISPR Products

miRNA for AKT1S1 Gene

miRTarBase miRNAs that target AKT1S1

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for AKT1S1

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for AKT1S1 Gene

Localization for AKT1S1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AKT1S1 Gene

Cytoplasm, cytosol. Note=Found in the cytosolic fraction of the brain. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for AKT1S1 Gene COMPARTMENTS Subcellular localization image for AKT1S1 gene
Compartment Confidence
cytosol 4
nucleus 2
extracellular 1

Gene Ontology (GO) - Cellular Components for AKT1S1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus --
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
GO:0031931 colocalizes_with TORC1 complex IDA 17386266
genes like me logo Genes that share ontologies with AKT1S1: view

Pathways for AKT1S1 Gene

genes like me logo Genes that share pathways with AKT1S1: view

Pathways by source for AKT1S1 Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for AKT1S1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006469 negative regulation of protein kinase activity IDA 17386266
GO:0007173 epidermal growth factor receptor signaling pathway TAS --
GO:0008543 fibroblast growth factor receptor signaling pathway TAS --
GO:0032007 negative regulation of TOR signaling IDA 17386266
GO:0034605 cellular response to heat TAS --
genes like me logo Genes that share ontologies with AKT1S1: view

Transcripts for AKT1S1 Gene

Unigene Clusters for AKT1S1 Gene

AKT1 substrate 1 (proline-rich):
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for AKT1S1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for AKT1S1 Gene

No ASD Table

Relevant External Links for AKT1S1 Gene

GeneLoc Exon Structure for
AKT1S1
ECgene alternative splicing isoforms for
AKT1S1

Expression for AKT1S1 Gene

mRNA expression in normal human tissues for AKT1S1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for AKT1S1 Gene

SOURCE GeneReport for Unigene cluster for AKT1S1 Gene Hs.515542

mRNA Expression by UniProt/SwissProt for AKT1S1 Gene

Q96B36-AKTS1_HUMAN
Tissue specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
genes like me logo Genes that share expressions with AKT1S1: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for AKT1S1 Gene

Orthologs for AKT1S1 Gene

This gene was present in the common ancestor of chordates.

Orthologs for AKT1S1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia AKT1S1 35
  • 98.96 (n)
  • 99.61 (a)
AKT1S1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia AKT1S1 35
  • 88.54 (n)
  • 94.53 (a)
AKT1S1 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia AKT1S1 35
  • 89.98 (n)
  • 93.84 (a)
AKT1S1 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Akt1s1 35
  • 83.59 (n)
  • 90.23 (a)
Akt1s1 16
Akt1s1 36
  • 93 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia AKT1S1 36
  • 64 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Akt1s1 35
  • 82.81 (n)
  • 89.84 (a)
lizard
(Anolis carolinensis)
Reptilia AKT1S1 36
  • 55 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.7177 35
tropical clawed frog
(Silurana tropicalis)
Amphibia akt1s1 35
  • 62.06 (n)
  • 60.22 (a)
Str.8926 35
zebrafish
(Danio rerio)
Actinopterygii akt1s1 36
  • 27 (a)
OneToOne
Species with no ortholog for AKT1S1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for AKT1S1 Gene

ENSEMBL:
Gene Tree for AKT1S1 (if available)
TreeFam:
Gene Tree for AKT1S1 (if available)

Paralogs for AKT1S1 Gene

No data available for Paralogs for AKT1S1 Gene

Variants for AKT1S1 Gene

Sequence variations from dbSNP and Humsavar for AKT1S1 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type MAF
rs1052068 -- 49,871,689(-) CCCCG(C/T)CGGCC reference, missense
rs2290774 -- 49,871,778(+) TGCCC(C/T)CAGGT intron-variant
rs2290775 -- 49,871,985(+) CACCA(C/T)CTCCA intron-variant
rs3745483 -- 49,869,610(+) AGGCG(A/G)AAAAC utr-variant-3-prime
rs3745484 -- 49,869,840(+) CAGAT(G/T)AGCAG utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for AKT1S1 Gene

Variant ID Type Subtype PubMed ID
nsv9739 CNV Gain+Loss 18304495
nsv912269 CNV Loss 21882294
dgv53n68 CNV Loss 17160897
nsv912272 CNV Loss 21882294
dgv3974n71 CNV Loss 21882294
dgv54n68 CNV Loss 17160897
nsv526372 CNV Loss 19592680
nsv524068 CNV Loss 19592680
nsv2519 CNV Loss 18451855
esv2718724 CNV Deletion 23290073
esv2718725 CNV Deletion 23290073
nsv138068 CNV Loss 16902084

Relevant External Links for AKT1S1 Gene

HapMap Linkage Disequilibrium report
AKT1S1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for AKT1S1 Gene

Disorders for AKT1S1 Gene

Relevant External Links for AKT1S1

Human Genome Epidemiology (HuGE) Navigator
AKT1S1
genes like me logo Genes that share disorders with AKT1S1: view

No data available for UniProtKB/Swiss-Prot for AKT1S1 Gene

Publications for AKT1S1 Gene

  1. Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. (PMID: 12524439) Kovacina K.S. … Roth R.A. (J. Biol. Chem. 2003) 2 3 4
  2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  4. Expression of proline-rich Akt-substrate PRAS40 in cell survival pathway and carcinogenesis. (PMID: 16174443) Huang B. … Porter G. (Acta Pharmacol. Sin. 2005) 3 4
  5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen J.V. … Mann M. (Cell 2006) 3 4

Products for AKT1S1 Gene

Sources for AKT1S1 Gene

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