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AKT1 Gene

protein-coding   GIFtS: 79

GC14M104361
v-akt murine thymoma viral oncogene homolog 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
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Aliases &
Descriptions
for AKT1

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
AKT 1, 2
C-AKT 3
EC 2.7.11.1 3
MGC99656 2
PKB 1, 2, 3
PKB-ALPHA 2
PRKBA 1, 2
RAC 1, 2, 3
RAC-ALPHA 2
RAC-PK-alpha 3
Descriptions
AKT1 kinase 2
Protein kinase B 2, 3
RAC-alpha serine/threonine-protein kinase 2
rac protein kinase alpha 2
v-akt murine thymoma viral oncogene homolog 1 2
External Ids
HGNC: 3911
Entrez Gene: 2072
UniProtKB: P317493
Ensembl: ENSG000001422087
Search outside databases for aliases for AKT1 gene

Previous GC identifers: GC14M102614 GC14M099226 GC14M103207

Summaries
for AKT1

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

 EntrezGene summary for AKT1:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in
serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by
platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by
mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs
through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator
of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a
transcription-independent manner by activating the serine/threonine kinase AKT1, which then
phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively
spliced transcript variants have been found for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: AKT1_HUMAN, P31749
Function: General protein kinase capable of phosphorylating several known proteins. Phosphorylates
TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of
various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor
(EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by
mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface.
Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly
by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced
activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced
activation of glycogen synthase

Gene Wiki entry for AKT1

Genomic Location
for AKT1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AKT1 gene  

Entrez Gene cytogenetic band: 14q32.32|14q32.32   Ensembl cytogenetic band:  14q32.33   HGNC cytogenetic band: 14q32.32-q32.33

AKT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M104361:     (about GC identifiers)

Start:
 104,306,731 bp from pter
End:
 104,333,125 bp from pter
Size:
 26,395 bases
Orientation:
 minus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  

Proteins
for AKT1

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: AKT1_HUMAN, P31749 (See protein sequence)
Recommended Name: RAC-alpha serine/threonine-protein kinase  
Size: 480 amino acids; 55686 Da
Subunit: Interacts with AGAP2 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The
C-terminus interacts with CCDC88A/GRDN and THEM4. Interacts with AKTIP. Interacts (via PH domain)
with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B
promoting 14-3-3 binding and cell-cycle progression
Subcellular location: Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by
integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with
TCL1A
PDB structures from and Proteopedia :
1H10 (3D)    1UNP (3D)    1UNQ (3D)    1UNR (3D)    2UVM (3D)    2UZR (3D)    2UZS (3D)    3CQU (3D)    
3CQW (3D)    
Secondary accessions: Q9BWB6

Post-translational modifications:

  • Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473
    phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473
    phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation
    is enhanced in focal cortical dysplasias with Taylor-type balloon cells1
  • View phosphorylation sites using PhosphoSite2


    • REFSEQ proteins (3 alternative transcripts): 
    NP_001014431.1  NP_001014432.1  NP_005154.2  


    ENSEMBL proteins: 
    ENSP00000270202 ENSP00000385326 ENSP00000384293 


    Human Recombinant Proteins 
    Invitrogen human recombinant proteins for AKT1
    Millipore Purified and/or Recombinant AKT1 Protein
    Sigma-Aldrich Proteins for AKT1  
    R&D Systems Recombinant & Natural Proteins for AKT1
    Enzo Life Sciences proteins for AKT1
    Recombinant Proteins from Abcam (AKT1, AKT , AKT1/2/3, AKT1/3, AKT)
    Human Recombinant Proteins from Abnova (AKT1)
                  OriGene Purified Recombinant Human Protein: AKT1 

    5/8 Gene Ontology (GO) cellular component terms (links to tree view) (see all 8 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005654 nucleoplasm EXP9829964 10490848 11274386
    GO:0005737 cytoplasm ISS--
    GO:0005819 spindle IEA--
    GO:0005829 cytosol EXP9381178 9812896 10485710 10698680 11715018 12167717 12244303 12524439 15183529 15718470
    About this table

    Antibodies for AKT1: 
    Invitrogen Antibodies for AKT1
    Millipore Mono- and Polyclonal Antibodies for the study of AKT1
    Sigma-Aldrich Antibody Arrays and Antibodies for AKT1
    R&D Systems Antibodies for AKT1
    Cell Signaling Technology (CST) Antibodies for AKT1 
    Antibodies from Abcam (AKT1, AKT , AKT1/2/3, AKT1/3, AKT), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (AKT1)
    Novus Biologicals Antibodies for AKT1

    Assays for AKT1: 
    Invitrogen Assays for AKT1
    Millipore Kits and Assays for the Analysis of AKT1
    R&D Systems ELISAs for AKT1         (see all)
    Cell Signaling Technology (CST) Sandwich ELISA Kits for AKT1
    Browse biochemical assays available from Enzo Life Sciences

    Protein Domains/
    Families
    for AKT1

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    5/10 InterPro domains/families (see all 10 ):
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser_thr_pkinase
     IPR015744 Serine/threonine_Kinase_Rac
     IPR017442 Se/Thr_pkinase-rel
     IPR000961 AGC-kinase_C


       GeneDecks  AKT1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P31749

    ProtoNet protein and cluster: P31749

    2 Blocks protein families:
    IPB000961 Protein kinase C-terminal domain
    IPB001849 Pleckstrin-like


    UniProtKB/Swiss-Prot: AKT1_HUMAN, P31749
    Domain: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its
    targeting to the plasma membrane
    Domain: The AGC-kinase C-terminal mediates interaction with THEM4
    Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC
    subfamily
    Similarity: Contains 1 AGC-kinase C-terminal domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 protein kinase domain

    Gene Function
    for AKT1

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (AKT1)
    Millipore RNAi Products for the Analysis of AKT1 Gene knock-down
    Abnova Chimera RNAi Products for Gene knock-down (AKT1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_001014431

                  Applied Biosystems Silencer® siRNAs for AKT1

                  Sigma-Aldrich siRNA and siRNA Panels for AKT1  
                         Sigma-Aldrich shRNA Panels and shRNA for AKT1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for AKT1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001014431
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001014431
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001014431 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001014431

    UniProtKB/Swiss-Prot: AKT1_HUMAN, P31749
    Function: General protein kinase capable of phosphorylating several known proteins. Phosphorylates
    TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of
    various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor
    (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by
    mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface.
    Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly
    by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced
    activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced
    activation of glycogen synthase
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Three specific sites, one in the kinase domain (Thr-308) and the two other ones
    in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full
    activation
    Enzyme Number (IUBMB): EC 2.7.11.1 

    Genatlas biochemistry entry for AKT1:
    V-akt murine thymoma viral oncogen homolog 1,general mediator of cell survival induced by insulin
    growth factor 1,AKT1 and others activated by phosphadidylinositol 3-kinase in the antiapoptotic
    PIK3 pathway,phosphorylating and inactivating components of the apoptotic machinery including
    BAD,CASP9 and the transcription factor FKHRL1

    15/21 MGI mutant phenotypes (inferred from 5 alleles) (MGI details for Akt1) (see all 21 ):

    behavior/neurologicalcellularembryogenesisendocrine/exocrine gland
    growth/sizehematopoietic systemhomeostasis/metabolismimmune systemlethality-postnatal
    lethality-prenatal/perinatallife span-post-weaning/agingliver/biliary systemmusclenervous system

    5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0004674 protein serine/threonine kinase activity IDA11994271 16139227
    GO:0005351 sugar:hydrogen symporter activity IEA--
    GO:0005524 ATP binding IC11994271
    GO:0016740 transferase activity IEA--
    About this table

    Pathways & Interactions
    for AKT1

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    5/49 Invitrogen iPath™ Online BioAtlas - Pathways for AKT1 (see all 49 ) (Maps provided by GeneGo):
     Putative integrins pathway. Part 2
     PTEN pathway
     Pleckstrin homology proteins interactions. Part VIII
     IL4 signaling pathway
     PDGF signaling via STATs and NF-kB

       GeneDecks  AKT1 for the pathways selected above  
    About GeneDecksing

    5/67 Millipore Pathways for AKT1 (see all 67 )
     Cytoskeleton remodeling FAK signaling
     G-protein signaling G-Protein beta/gamma signaling cascades
     Transcription NF-kB signaling pathway
     Development A2A receptor signaling
     Immune response IL-4 signaling pathway

       GeneDecks  AKT1 for the pathways selected above  
    About GeneDecksing

    5/81 Sigma-Aldrich "Your Favorite Gene" Pathways for  AKT1  (Your Favorite Gene powered by Ingenuity) (see all 81 ) 
     Human Embryonic Stem Cell Pluripotency
     IL-15 Signaling
     RAR Activation
     Insulin Receptor Signaling
     IL-17 Signaling

       GeneDecks  AKT1 for the pathways selected above  
    About GeneDecksing

    5/26 Kegg Pathways  (Kegg details for AKT1) (see all 26 ):
     hsa04010 MAPK signaling pathway
     hsa04012 ErbB signaling pathway
     hsa04150 mTOR signaling pathway
     hsa04210 Apoptosis
     hsa04370 VEGF signaling pathway

       GeneDecks  AKT1 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  AKT1 


    5/568 Interacting proteins for AKT1 (P317491, 2 ENSP000002702023) via UniProtKB, MINT, and/or STRING (see all 568 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    STK4Q130432, ENSP000003618923MINT-6175011 MINT-6175193 MINT-6175251 MINT-6175070 MINT-6175034 MINT-6174722 MINT-6175225 MINT-6175132 MINT-6175047 MINT-6175025 MINT-6175115 MINT-6175085 MINT-6175239 STRING (score=.702)
    PLCG1P191742, ENSP000003623693MINT-7146749 MINT-7146647 MINT-7146716 MINT-7146688 MINT-7146767 MINT-7146702 MINT-7146657 MINT-7146671 STRING (score=.814)
    MDM2Q009872, ENSP000002581493MINT-6823537 MINT-50904 MINT-50905 STRING (score=.999)
    CDKN1BP465272, ENSP000002288723MINT-17680 MINT-17679 STRING (score=.999)
    PPP2CAP677752, ENSP000002315043MINT-74931 MINT-74941 STRING (score=.984)
    About this table

    5/46 Gene Ontology (GO) biological process terms (links to tree view) (see all 46 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000060 protein import into nucleus, translocation IMP16280327
    GO:0001568 blood vessel development IEA--
    GO:0001890 placenta development IEA--
    GO:0005975 carbohydrate metabolic process IEA--
    GO:0006006 glucose metabolic process IEA--
    About this table

    Drugs & Compounds
    for AKT1

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for AKT1
    Sigma-Aldrich Small Molecules for AKT1:
    Inhibitor

    Compounds for AKT1 available from Tocris Bioscience
    CompoundAction CAS number
    10-DEBC hydrochlorideSelective Akt/PKB inhibitor[201788-90-1]
    API-2Selective inhibitor of Akt/PKB signaling. Antitumor and antiviral[35943-35-2]
    FPA 124Akt/PKB inhibitor[902779-59-3]
    About this table


    10/29 Novoseek chemical compound relationships for AKT1 gene (see all 29 )
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    phosphatidylinositol 88.47 234 12551955 (5), 10578066 (5), 8645157 (3), 10671514 (3) (see all 99)
    wortmannin 83.87 87 17380453 (3), 16458614 (2), 9346964 (2), 9755080 (2) (see all 62)
    phosphoinositide 83.69 118 10454216 (4), 10102683 (3), 9671484 (2), 15292195 (2) (see all 82)
    phosphatidylinositol-3,4,5-trisphosphate 77.90 19 7738099 (1), 15107457 (1), 9228007 (1), 12421919 (1) (see all 17)
    threonine 77.43 105 10362672 (1), 10411946 (1), 10491192 (1), 11279265 (1) (see all 96)
    glycogen 70.67 110 11574400 (5), 10623881 (4), 9478990 (3), 10446195 (2) (see all 67)
    tyrosine 68.72 98 9593727 (3), 7616102 (3), 9415029 (3), 10623881 (2) (see all 69)
    gtp 59.01 54 7493928 (3), 8485230 (2), 8663331 (2), 12955089 (2) (see all 33)
    gdp 58.61 23 18505730 (2), 8910315 (2), 8810315 (1), 16049005 (1) (see all 19)
    geranylgeranyl pyrophosphate 58.46 3 17266583 (1), 18658277 (1)
    About this table

    3 PharmGKB drug compound relationships for AKT1 gene
    Drug compound PharmGKB Relations PubMed IDs for articles supporting these relationships
    arsenic trioxideFA  15316930
    nicotineFA  12511591
    xenobioticsFA  12511591
    About this table


    Transcripts
    for AKT1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (AKT1)
    Millipore RNAi Products for the Analysis of AKT1 Gene knock-down
    Abnova Chimera RNAi Products for Gene knock-down (AKT1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_001014431

                  Sigma-Aldrich siRNA and siRNA Panels for AKT1  
                         Sigma-Aldrich shRNA Panels and shRNA for AKT1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001014431  NM_001014432  NM_005163  

    REFSEQ mRNAs for AKT1 gene (3 alternative transcripts): 

    NM_001014431.1   NM_001014432.1   NM_005163.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001014431  NM_001014432  NM_005163  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001014431
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001014431
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001014431 

    Additional cDNA sequence: 

    AB451242.1 AB451367.1 AK122894.1 AK299310.1 AK314256.1 BC000479.2 BC001737.1 BC063408.1 
    BC084538.1 BX648205.1 CR611982.1 M63167.1 X61037.1 

    24/25 DOTS entries (see all 25 ):

    DT.450038  DT.100059722  DT.100824191  DT.100059719  DT.95090241  DT.100733482  DT.120765970  DT.92461206 
    DT.100824203  DT.102829546  DT.75100119  DT.95341680  DT.100824190  DT.100824201  DT.120765944  DT.95090231 
    DT.95090239  DT.95090245  DT.120765955  DT.120765973  DT.92461194  DT.92461227  DT.95124889  DT.100059721 

    13 AceView cDNA sequences:

    BC001737 BC000479 BX648205 CR611982 AK131465 M63167 BX647722 AK094287 
    AK122894 NM_005163 AK127193 X61037 BC063408 

    highest scoring ESTs for AKT1:

    BC000479 M63167 AA349777 AA349778 AA444055 AA460546 AA573336 AA622342 AA716185 AA746277 

    Unigene Cluster for AKT1:

    V-akt murine thymoma viral oncogene homolog 1
    Hs.525622  [show with all ESTs]
    Unigene Representative Sequence: AK122894


    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for AKT1 (see all 10 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c ^ 10a · 10b · 10c ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14a · 14b ^ 15 ^
    SP1:                                                                                      -     -                                                               
    SP2:                                                                                      -     -                                                           -   
    SP3:                                                                                      -     -                                                               
    SP4:                                                                                -     -     -                                                               
    SP5:                                                                                                                                -                       -   

    ExUns: 16 ^ 17a · 17b ^ 18
    SP1:                        
    SP2:                        
    SP3:                        
    SP4:                        
    SP5:                        

    About this scheme

    ECgene alternative splicing isoforms for AKT1

    3 Ensembl transcripts including schematic representations:
    ENST00000349310  ENST00000402615  ENST00000407796  

    Expression
    for AKT1

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    AKT1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for AKT1

    1 / 2 / 3

    4 probe-sets matching AKT1 gene


    		Affymetrix
    probe-set    		 Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    		1564_at2, 3 U95-A 1 1.00 1.00 0.44 1.00 M63167 0.80 1.00 0.91 1

    		67939_at2, 3 U95-E 1 0.88 1.00 0.87 1.00 AA962189 0.40 1.00 0.76 1

    		207163_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005163 0.60 1.00 0.82 1

    		207163_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.525622

    Expression variation in blood from EXPOLDB for AKT1

    UniProtKB/Swiss-Prot: AKT1_HUMAN, P31749
    Tissue specificity: In all human cell types so far analyzed

    Orthologs
    for AKT1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section
    Orthologs for AKT1 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity    		 NCBI accessions
    dog
    (Canis familiaris)    		 AKT11   -- v-akt murine thymoma viral oncogene homolog 1 91.33(n)
    96.99(a)    		 490878  XM_548000.2  XP_548000.2 
    chimpanzee
    (Pan troglodytes)    		 AKT11   -- v-akt murine thymoma viral oncogene homolog 1 98.78(n)
    99.21(a)    		 740898  XM_001143158.1  XP_001143158.1 
    cow
    (Bos taurus)    		 AKT11   -- v-akt murine thymoma viral oncogene homolog 1 90.83(n)
    96.25(a)    		 280991  NM_173986.2  NP_776411.1 
    rat
    (Rattus norvegicus)    		 Akt11   -- v-akt murine thymoma viral oncogene homolog 1 90.9(n)
    98.12(a)    		 24185  NM_033230.1  NP_150233.1 
    mouse
    (Mus musculus)    		 Akt11, 5 12 (57.00 cM)5
    		 thymoma viral proto-oncogene 11, 5 90.9(n)1
    98.33(a)1    		 116511  NM_009652.21  NP_033782.11 
     AF5341345  AK0407585  (see all 17)
    About this table        Species with no ortholog for AKT1

    ENSEMBL Gene Tree for AKT1

    Paralogs
    for AKT1

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for AKT1 gene
    AKT32  AKT22  

    SNPs/Variants
    for AKT1

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/309 NCBI SNPs in AKT1 are shown (see all 309 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 101)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 14 posSequenceRecsAA
    Chg    		TypeMoreRecsAllele
    freq    		PopTotal
    sample    		More
    ------------
    rs11302141,2
    		A,C,F,H104330779(-) TTTTGG/TGCTTG 3 -- int1 ut519Minor allele frequency- T:0.24EU EA WA 928
    rs24947501,2
    		A,C,F,H104333957(+) CAGGAC/GAGTGC 2 -- ng318Minor allele frequency- G:0.38EU EA WA NA 776
    rs101382271,2
    		C,F,H104330751(+) GCAAAC/TGGGAG 3 -- int1 ut514Minor allele frequency- T:0.13EU EA 398
    rs24947511,2
    		A,C104334006(+) CCTCCA/GACAAC 2 -- ng310--------
    rs24947521,2
    		A,C104334653(+) AGGGTA/GAGAGG 2 -- ng310--------
    rs11302451,2
    		C,F104307400(-) GGGCCG/AGGCAG 3 -- ut31 ese35Minor allele frequency- A:0.01MN CSAM EA 396
    rs286349991,2
    		C,F104331346(+) TTAGAC/TGCTCT 3 -- int1 ng311Minor allele frequency- T:0.05MN 706
    rs24987861,2
    		A,C104333413(-) GTCACC/GGCAGA 2 -- ng31 tfbs30--------
    rs24988011,2
    		A,C104306603(-) CCCCAA/GCAGGT 3 -- ng510--------
    --
    rs115554331,2
    		H104312525(-) CCTGGT/CGAAGG 3 A/V mis14Minor allele frequency- C:0.00EU EA WA 404
    About this table

    HapMap Linkage Disequilibrium images for AKT1 (up to first 250kb)

    Disorders & Mutations
    for AKT1

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    		 OMIM: 164730   disorders: 114480  114500  604370  181500  

    UniProtKB/Swiss-Prot: AKT1_HUMAN, P31749

  • Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely
    common malignancy, affecting one in eight women during their lifetime
  • Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500]
  • Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also
    called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1)

    • 10/81 Novoseek disease relationships for AKT1 gene (see all 81 )

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 49.15 81 11571628 (5), 19216065 (3), 17611497 (3), 12191616 (2) (see all 54)
    insulin resistance 47.52 13 15634339 (2), 10373517 (1), 11257494 (1), 12663464 (1) (see all 12)
    wiskott-aldrich syndrome 46.80 7 15657136 (1), 10488099 (1), 18198193 (1), 11580754 (1) (see all 7)
    tumors 39.11 122 17027002 (4), 12191616 (4), 11593437 (4), 17639041 (4) (see all 65)
    glioblastoma 33.32 7 10958682 (2), 11439092 (1), 18250332 (1), 19234490 (1) (see all 5)
    breast cancer 32.90 45 16782806 (5), 16711509 (3), 16428439 (3), 18087195 (3) (see all 25)
    insulin sensitivity 25.73 2 18599621 (1), 18573875 (1)
    hyperinsulinemia 25.05 1 17513702 (1)
    metastasis 22.53 13 19305425 (2), 15886842 (2), 16618711 (1), 17234445 (1) (see all 9)
    thymoma 19.22 8 16251502 (1), 15522255 (1), 17464696 (1), 17611497 (1) (see all 6)
    About this table

    2 PharmGKB disease relationships for AKT1 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    LeukemiaFA  15316930
    Lung NeoplasmsFA  12511591
    About this table

    Human Gene Mutation Database: AKT1
    Genetic Association Database: AKT1
    Human Genome Epidemiology Navigator: AKT1 (32 documents)
    Tumor Gene Database: AKT1

    Medical News
    for AKT1

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    Publications
    for AKT1

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    		10/1928 PubMed articles for AKT1 gene (see all 1928 ):

    Search for AKT1

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing AKT1

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section
    Entrez Gene: 207 HGNC: 391 AceView: AKT1 Ensembl:ENSG00000142208 euGenes: HUgn207
    ECgene: AKT1 H-InvDB: AKT1

    Other Databases showing AKT1

    (According to HUGE)
    About This Section

    		   --

    Specialized Databases showing AKT1

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    ATLAS Chromosomes in Cancer entry for AKT1 Genetics and Cytogenetics in Oncology and Haematology

    Licensable Technologies
    for AKT1

    (Available from WIS Yeda, Salk, Tufts)
    About This Section

    		Licensable Technologies for AKT1 gene:
     Salk Institute: Protein Kinase B/AKT Modulators and Methods of Use Thereof
     Tufts University:  Regulation of Oncogenesis by Akt-Specific Isoforms

    Services
    for AKT1

    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for AKT1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
    		  Free SNP selection tool



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      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for AKT1-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


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     Lentivirus-delivered shRNAs for AKT1 Small molecules for AKT1
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

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     Antibodies (AKT1)
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     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Antibodies & Assays for AKT1 

     Recombinant Proteins
    (AKT1, AKT , AKT1/2/3, AKT1/3, AKT)
     Antibodies (AKT1, AKT , AKT1/2/3, AKT1/3, AKT)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Tocris compounds for AKT1




     Drugs/Proteins for AKT1
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for AKT1

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