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AKNA Gene

protein-coding   GIFtS: 49

GC09M116138
AT-hook transcription factor
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLJ31001 2
FLJ33184 2
KIAA1968 1, 2, 3
OTTHUMP00000021973 2
RP11-82I1.4 2
Descriptions
AKNA transcript F2 2
AT-hook transcription factor 2
AT-hook transcription factor AKNA 2
External Ids
HGNC: 241081
Entrez Gene: 807092
UniProtKB: Q7Z5913
Ensembl: ENSG000001069487
Search outside databases for aliases for AKNA gene

Previous GC identifers: GC09M107896 GC09M108827 GC09M110552 GC09M112474 GC09M114177

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: AKNA_HUMAN, Q7Z591
Function: Transcription factor that specifically activates the expression of the CD40 receptor and
its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for
antigen-dependent-B-cell development. Binds to A/T-rich promoters

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AKNA gene  

Entrez Gene cytogenetic band: 9q32   Ensembl cytogenetic band:  9q32   HGNC cytogenetic band: 9q32

AKNA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09M116138:     (about GC identifiers)

Start:
116,136,250 bp from pter
End:
116,196,506 bp from pter
Size:
60,257 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000009.10  NT_008470.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: AKNA_HUMAN, Q7Z591 (See protein sequence)
Recommended Name: AT-hook-containing transcription factor  
Size: 1439 amino acids; 155139 Da
Subcellular location: Nucleus
Sequence caution: Sequence=AAK83024.1; Type=Frameshift; Positions=1228; Sequence=AAU34192.1;
Type=Frameshift; Positions=1227; Sequence=BAC85132.1; Type=Frameshift; Positions=1340;
Sequence=BAD18725.1; Type=Frameshift; Positions=820, 1017, 1340, 1387, 1434;
Secondary accessions: Q05BK5 Q5T535 Q5T536 Q5T537 Q64FX6 Q64FX7 Q64FX8 Q64FY2 Q6ZMK0 Q6ZNL2 Q6ZTX0
Q8TET1 Q8TF33 Q96RR9 Q9H7P7
Alternative splicing: 8 isoforms:  Q7Z591-1   Q7Z591-2   Q7Z591-3   Q7Z591-4   Q7Z591-5   Q7Z591-6   Q7Z591-7   Q7Z591-8   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_110394.3  

    ENSEMBL proteins: 
    ENSP00000223791 ENSP00000314538 ENSP00000363187 ENSP00000309222 ENSP00000378075 ENSP00000363188 
    ENSP00000378083 ENSP00000363201 ENSP00000303769 ENSP00000363192 


    Human Recombinant Proteins 
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    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (AKNA)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for AKNA: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for AKNA

    Assays for AKNA: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q7Z591

    UniProtKB/Swiss-Prot: AKNA_HUMAN, Q7Z591
    Similarity: Contains 1 A.T hook DNA-binding domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AKNA)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030767

                  Applied Biosystems Silencer® siRNAs for AKNA

                  Sigma-Aldrich siRNA for AKNA  
                         Sigma-Aldrich shRNA for AKNA  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030767
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030767
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_030767 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_030767

    UniProtKB/Swiss-Prot: AKNA_HUMAN, Q7Z591
    Function: Transcription factor that specifically activates the expression of the CD40 receptor and
    its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for
    antigen-dependent-B-cell development. Binds to A/T-rich promoters

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    4 Interacting proteins for AKNA (ENSP000003631923 Q7Z5912) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CD2BP2O954002MINT-62658 MINT-62647
    AHDC1ENSP000002470873STRING (score=.862)
    CD40ENSP000002790613STRING (score=.725)
    --O751372MINT-65908
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for AKNA

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AKNA)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030767

                  Sigma-Aldrich siRNA for AKNA  
                         Sigma-Aldrich shRNA for AKNA  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_030767  

    REFSEQ mRNAs for AKNA gene: 

    NM_030767.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_030767  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030767
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030767
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_030767 

    Additional cDNA sequence: 

    AB075848.1 AF286341.1 AK024431.1 AK055563.1 AK074040.1 AK098036.1 AK131082.1 AK160382.1 
    AL833220.1 AY703039.1 AY703040.1 AY703041.1 AY703042.1 AY703043.1 AY703044.1 AY703045.1 
    AY703046.1 BC042202.1 BC055285.1 

    20 DOTS entries:

    DT.65286353  DT.99991191  DT.312555  DT.101984182  DT.97845363  DT.91883868  DT.86849275  DT.91797955 
    DT.121186626  DT.91712323  DT.91757913  DT.100751581  DT.95196176  DT.100751582  DT.86846849  DT.95272972 
    DT.99929870  DT.101984181  DT.91938389  DT.97803439 

    24/161 AceView cDNA sequences (see all 161 ):

    AY703044 AB075848 CA432838 BM142077 AK024431 BF940396 CK819880 AI825795 
    BC042202 BC055285 BQ636860 AK126136 AL833220 BF057373 NM_030767 AY703043 
    CA433586 AY703040 AY703042 AK074040 AW502370 BX100125 AI217173 BG471175 

    highest scoring ESTs for AKNA:

    AK126136 BC055285 BU170540 T65933 AA363272 AA363273 AA452711 AA482620 AA495919 AA495920 

    Unigene Cluster for AKNA:

    AT-hook transcription factor
    Hs.494895  [show with all ESTs]
    Unigene Representative Sequence: NM_030767


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for AKNA (see all 7 )

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b · 13c · 13d ^ 14 ^ 15 ^ 16a · 16b ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^
    SP1:                                                                                                                                                            
    SP2:                          -                                                                                                                                 
    SP3:                                                                                                                                                            
    SP4:                                                                                            -     -                                                         
    SP5:                                                                                                                                                            

    ExUns: 22 ^ 23 ^ 24 ^ 25 ^ 26
    SP1:                              
    SP2:                              
    SP3:                              
    SP4:                              
    SP5:                              

    About this scheme

    ECgene alternative splicing isoforms for AKNA

    10 Ensembl transcripts including schematic representations:
    ENST00000223791  ENST00000320310  ENST00000374074  ENST00000312033  ENST00000394574  ENST00000374075  
    ENST00000394582  ENST00000374088  ENST00000307564  ENST00000374079  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    AKNA expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for AKNA

    1 / 2 / 3

    4 probe-sets matching AKNA gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    59388_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 T65933 0.80 1.00 0.91 1

    225701_at2, 3 U133-B 1 1.00 1.00 -- -- AK024431 0.60 1.00 0.82 1

    225701_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1558565_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  AKNA for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CCTGAACTCT

    SOURCE GeneReport for Unigene cluster: Hs.494895

    UniProtKB/Swiss-Prot: AKNA_HUMAN, Q7Z591
    Tissue specificity: Predominantly expressed by lymphoid tissues. Highly expressed in the spleen,
    lymph nodes and peripheral blood leukocytes, expressed at lower level in the thymus. Mainly
    expressed by germinal center B-lymphocytes, a stage in which receptor and ligand interactions are
    crucial for B-lymphocyte maturation. Expressed by B- and T-lymphocytes, Natural killer cells and
    CD1a(+)CD14(-) but not CD1a(-)CD14(+) dendritic cells. Weakly or not expressed in fetal liver and
    in adult bone marrow

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for AKNA gene from 3 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    rat
    (Rattus norvegicus)
    Akna1   -- AT-hook transcription factor 71.88(n)
    59.69(a)
    362530  XM_342848.3  XP_342849.3 
    mouse
    (Mus musculus)
    Akna1, 5 45
    AT-hook transcription factor1, 5 71.81(n)1
    60.19(a)1
    1001821  NM_001045514.11  NP_001038979.11 
     AI5970135  AK0297015  (see all 18)
    zebrafish
    (Danio rerio)
    Dr.164132   -- Transcribed sequences 76.17(n)  BI562789.1 
    About this table        Species with no ortholog for AKNA

    ENSEMBL Gene Tree for AKNA
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/385 NCBI SNPs in AKNA are shown (see all 385 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 163)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 9 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10445311,2
    A,C,F,H,O116138471(+) ATAAAT/CGCCAC 1 -- ut3121Minor allele frequency- C:0.04EA NA MN EU WA 1801
    rs37481761,2
    A,C,F,H116164552(-) CCAGCC/TGCTTG 1 P/L mis1 ese39Minor allele frequency- T:0.40EA EU WA 2334
    rs22502421,2
    A,C,F,H116143794(+) TGAAGG/ATGCTT 1 P/S mis1 ese313Minor allele frequency- A:0.38EA EU WA NA 2698
    rs107394071,2
    A,C,F,H116137576(+) TGCTAT/CTGAAA 1 -- ut318Minor allele frequency- C:0.15EU EA WA 836
    rs12658911,2
    A,C,F,H116149933(+) GTGGCT/CGGTGC 1 R/Q mis112Minor allele frequency- C:0.16MN EU EA WA NA 1164
    rs169286921,2
    C,F,H116196549(+) TGAGGC/TCCTTT 1 -- ng3111Minor allele frequency- T:0.03NA EU EA WA 976
    rs109821671,2
    C,F,H116136741(+) ACCTGG/AGTGCT 1 -- ut314Minor allele frequency- A:0.19EU EA WA 392
    rs27873441,2
    C,F,H116143721(+) CCAGAT/CACCAC 1 C/Y mis1 ese38Minor allele frequency- C:0.12EU EA WA 840
    rs26368691,2
    A,C,H116138422(+) TGGCCG/ACAGAC 1 -- ut31 ese33Minor allele frequency- A:0.00EU EA 296
    rs108176001,2
    A,F,H116198318(+) CACAGC/TGGCTA 1 -- ng314Minor allele frequency- T:0.47EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for AKNA (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605729

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    8 PubMed articles for AKNA gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 80709 HGNC: 24108 AceView: AKNA Ensembl:ENSG00000106948 euGenes: HUgn80709
    ECgene: AKNA H-InvDB: AKNA
    (According to HUGE)
    About This Section

    HUGE: KIAA1968
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for AKNA:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for AKNA Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for AKNA Browse small molecules at Sigma-Aldrich
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