AKAP8 Gene
protein-coding GIFtS: 61
GCID: GC19M015464
|
|
A kinase (PRKA) anchor protein 8
| |
Aliases
for AKAP8 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| A Kinase (PRKA) Anchor Protein 81 2 | | AKAP-952 | | AKAP951 2 3 | | A-Kinase Anchor Protein 82 | | AKAP-82 3 | | A-Kinase Anchor Protein, 95kDa2 | | AKAP 952 3 | | A-Kinase Anchor Protein 95 KDa3 | | DKFZp586B12221 | | |
Export aliases for AKAP8 gene to outside databasesPrevious GC identifers: GC19M015843 GC19M015309 GC19M015325 GC19M015032 |
Summaries
for AKAP8 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for AKAP8:
This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins thatcontain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signalingmolecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RIIalpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensincomplex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. (provided by RefSeq, May2011)
UniProtKB/Swiss-Prot: AKAP8_HUMAN, O43823Function: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA typeII)
Gene Wiki entry for AKAP8
|
Genomic Views
for AKAP8 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000019.9 NC_018930.1 NT_011295.11
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the AKAP8 gene promoter:
Pax-5 p53 LUN-1 Nkx2-5 Tal-1beta FOXO3b HOXA5 ZID ITF-2 Pax-4a
Other transcription factors
Search SABiosciences Chromatin IP Primers for AKAP8
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AKAP8 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 19p13.1 Ensembl cytogenetic band: 19p13.12 HGNC cytogenetic band: 19p13.12AKAP8 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19M015464: view genomic region
(about GC identifiers)
Start:
|
15,464,335 bp from pter |
End:
|
15,490,603 bp from pter |
Size:
|
26,269 bases |
Orientation:
|
minus strand |
|
Proteins
for AKAP8 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: AKAP8_HUMAN, O43823 (See
protein sequence)Recommended Name: A-kinase anchor protein 8 Size: 692 amino acids; 76108 Da
Subunit: Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis. Interacts (via C-terminus) with FIGN (Bysimilarity)
Subcellular location: Nucleus matrix. Note=Associated with the nuclear matrix. Redistributed and detached fromcondensed chromatin during mitosisExplore the universe of human proteins at neXtProt for AKAP8: NX_O43823
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O43823
AKAP8 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_005849.1
ENSEMBL proteins: ENSP00000269701 ENSP00000442114
Human Recombinant Protein Products:
Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table
AKAP8 for ontologies About GeneDecksing
AKAP8 Antibody Products:
Assay Products for AKAP8: |
Protein
Domains /
Families
for AKAP8 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
AKAP8 for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry O43823ProtoNet protein and cluster: O43823 1 Blocks protein family: IPB007071 A-kinase anchoring protein 95 (AKAP95)
UniProtKB/Swiss-Prot: AKAP8_HUMAN, O43823Similarity: Belongs to the AKAP95 familySimilarity: Contains 2 C2H2 AKAP95-type zinc fingers |
Function
for AKAP8 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: AKAP8_HUMAN, O43823Function: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA typeII) Genatlas biochemistry entry for AKAP8:A kinase (PRKA regulatory subunit RIIA) anchor protein 8,located in the nucleus in quiescent fibroblasts,excludingRIIA,suggesting that the interaction is cell cycle dependent,homolog to rat AKAP95
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AKAP8
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AKAP8 |
Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table
AKAP8 for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for AKAP8:
Animal Models:
6 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Akap8):
AKAP8 for phenotypes About GeneDecksing
|
Pathways & Interactions
for AKAP8 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Activation of cAMP-Dependent PKA | | | 2 | G Protein Signaling Pathways | | | 3 | Cell cycle Chromosome condensation in prometaphase | | | 4 | TNF-alpha/NF-kB Signaling Pathway | |
Pathway sources
See GeneCards unified pathways
Show all pathways
2 EMD Millipore Pathways for AKAP8
4 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for AKAP8
2  GeneGo (Thomson Reuters) Pathways for AKAP8
2  BioSystems Pathways for AKAP8
AKAP8 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for AKAP8
STRING Interaction
Network Preview (showing 5 interactants - click image to see 17)
5/550 Interacting proteins for AKAP8 (O438232, 3 ENSP000002697014) via UniProtKB, MINT, STRING, and/or I2D (see all 550)About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
AKAP8 for ontologies About GeneDecksing
|
Drugs & Compounds
for AKAP8 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
AKAP8 for compounds About GeneDecksing
 Browse Tocris compounds for AKAP8
1 HMDB Compound for AKAP8 About this table
2 Novoseek chemical compound relationships for AKAP8 gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| threonine |
26.8 |
2 |
11591814 (2) |
| zinc |
14.5 |
3 |
11964380 (2), 10697960 (1) |
Search CenterWatch for drugs/clinical trials and news about AKAP8
|
Transcripts
for AKAP8 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for AKAP8 gene: NM_005858.3 Unigene Cluster for AKAP8: A kinase (PRKA) anchor protein 8 Hs.743430 [show with all ESTs]Unigene Representative Sequence: NM_0058582 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000269701(uc010dzy.3 uc002nav.3 uc010dzz.1) ENST00000537303(uc010xog.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for AKAP8 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AKAP8
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: AKAP8 (NM_005858) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for AKAP8 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AKAP8 |
Additional cDNA sequence: AK303640.1 AK308251.1 AK309529.1 AL050160.1 BC037270.2 Y11997.1 7 DOTS entries: DT.313147 DT.95265881 DT.97810454 DT.91866697 DT.40273193 DT.40205408 DT.95215098 24/447 AceView cDNA sequences (see all 447): AI089655 BC007551 BQ225239 BF768925 AW241407 AI971074 AI480412 AI192772 BE707178 AW014702 AI083553 AW071103 BE312479 BU741898 AI089392 AI417320 BX394833 BP366898 AI421203 AW103772 BI439161 CD675381 CK430553 BC002329
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for AKAP8 About this scheme
| ExUns: | 1a | · | 1b | · | 1c | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5a | · | 5b | ^ | 6a | · | 6b | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 | ^ | 15 |
|---|
|
| SP1: | | | | | | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | - | | | | | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | - | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for AKAP8
|
Expression
for AKAP8 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| AKAP8 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AGGAGCCTTA
About this image
See AKAP8 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for AKAP8
SOURCE GeneReport for Unigene cluster: Hs.743430
UniProtKB/Swiss-Prot: AKAP8_HUMAN, O43823Tissue specificity: Highly expressed in heart, liver, skeletal muscle, kidney and pancreas
SABiosciences Custom PCR Arrays for AKAP8
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for AKAP8
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat AKAP8 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat AKAP8 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat AKAP8 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AKAP8 |
Orthologs
for AKAP8 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for AKAP8 gene from 2/9 species (see all 9) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
AKAP81 |
A kinase (PRKA) anchor protein 8 |
67.95(n)
60.96(a) |
|
426167 XM_423831.3 XP_423831.3 |
lizard
(Anolis carolinensis) |
Reptilia |
AKAP86 |
-- |
55(a) |
1 ↔ 1 |
2(103420049-103441851) |
ENSEMBL Gene Tree for AKAP8 (if available)
TreeFam Gene Tree for AKAP8 (if available) |
Paralogs
for AKAP8 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for AKAP8 gene
- AKAP8L2 ZNF3262
2 SIMAP similar genes for AKAP8 using alignment to 4 protein entries: AKAP8_HUMAN (see all proteins):DKFZp586B1222 AKAP8L
AKAP8 for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for AKAP8
PGOHUM00000235963
|
Genomic Variants
for AKAP8 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 19 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for AKAP8 (15464335 - 15490603 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for AKAP8: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing AKAP8 |
|
Disorders
/ Diseases
for AKAP8 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
AKAP8 for disorders About GeneDecksing
OMIM gene information: 604692
OMIM disorders: --
2  diseases for AKAP8: About MalaCardspolycystic kidney disease kidney disease
Export disorders for AKAP8 gene to outside databases
|
Publications
for AKAP8 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for AKAP8 gene, integrated from 9 sources (see all 47):
(articles sorted by number of sources associating them with AKAP8) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. (PubMed id 9473338)1, 2, 3, 9 Eide T.... Tasken K. (1998)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (2006)
- The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)1, 2 Grimwood J.... Lucas S.M. (2004)
- A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract. (PubMed id 10791967)1, 9 Steen R.L....Collas P. (2000)
- Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting. (PubMed id 11964380)1, 9 Eide T....Collas P. (2002)
- A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95. (PubMed id 14641107)1, 9 Arsenijevic T....Pirson I. (2004)
- A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. (PubMed id 16227597)1, 9 Kamada S....Hunter T. (2005)
- AMY-1 interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex. (PubMed id 12414807)1, 9 Furusawa M....Ariga H. (2002)
- A-kinase anchoring proteins interact with phosphodiesterases in T lymphocyte cell lines. (PubMed id 15470020)1, 9 Asirvatham A.L.... Carr D.W. (2004)
- A-kinase anchoring in dendritic cells is required for antigen presentation. (PubMed id 19277197)1, 9 Schillace R.V....Carr D.W. (2009)
|
External Searches for AKAP8 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing AKAP8 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing AKAP8 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing AKAP8 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for AKAP8 gene:
Search GeneIP for patents involving AKAP8
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for AKAP8 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| |
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| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AKAP8 |
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