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Aliases for AKAP8 Gene

Aliases for AKAP8 Gene

  • A-Kinase Anchoring Protein 8 2 3 5
  • A Kinase (PRKA) Anchor Protein 8 2 3
  • A-Kinase Anchor Protein, 95kDa 2 3
  • AKAP 95 3 4
  • AKAP-8 3 4
  • AKAP95 3 4
  • A-Kinase Anchor Protein 95 KDa 4
  • A-Kinase Anchor Protein 8 3
  • AKAP-95 3

External Ids for AKAP8 Gene

Previous GeneCards Identifiers for AKAP8 Gene

  • GC19M015843
  • GC19M015309
  • GC19M015325
  • GC19M015464
  • GC19M015032

Summaries for AKAP8 Gene

Entrez Gene Summary for AKAP8 Gene

  • This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

GeneCards Summary for AKAP8 Gene

AKAP8 (A-Kinase Anchoring Protein 8) is a Protein Coding gene. Among its related pathways are Activation of cAMP-Dependent PKA and Signal transduction_PKA signaling. Gene Ontology (GO) annotations related to this gene include protein kinase A regulatory subunit binding. An important paralog of this gene is AKAP8L.

UniProtKB/Swiss-Prot for AKAP8 Gene

  • Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at Ser-10 of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 Lys-4 methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).

Gene Wiki entry for AKAP8 Gene

Additional gene information for AKAP8 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AKAP8 Gene

Genomics for AKAP8 Gene

GeneHancer (GH) Regulatory Elements for AKAP8 Gene

Promoters and enhancers for AKAP8 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19I015376 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 596.1 +0.5 527 5.5 HDGF PKNOX1 ARID4B SIN3A DMAP1 ZBTB7B YY1 SLC30A9 POLR2B ZNF766 AKAP8 ENSG00000268189 ZNF333 BRD4 TPM4 DDX39A AKAP8L GC19M015202
GH19I016063 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 86.7 -693.1 -693085 18.7 CLOCK FEZF1 DMAP1 IRF4 E2F8 ZNF143 ZNF263 MCM3 SP3 MEF2D TPM4 AKAP8 CHERP ENSG00000269578 C19orf44 BRD4 ENSG00000267904 ENSG00000268087 SMIM7 MED26
GH19I014378 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 79.4 +997.3 997252 7.7 HDGF PKNOX1 CLOCK SMAD1 ARNT SIN3A FEZF1 YBX1 ZNF2 IRF4 ADGRE5 CCDC130 AKAP8 MRI1 ZNF333 BRD4 DDX39A DCAF15 RFX1 LOC284454
GH19I016110 Promoter/Enhancer 2.8 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 65 -736.3 -736347 11 MLX DMAP1 YBX1 IRF4 YY1 E2F8 ZNF143 ZNF548 SP3 NFYC RAB8A CHERP AKAP8 BRD4 ENSG00000268743 ENSG00000269578 HAUS8 C19orf44 ENSG00000267904 ENSG00000268087
GH19I015123 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 47.3 +254.6 254576 3.7 HDGF PKNOX1 CLOCK MLX ARNT ARID4B SIN3A DMAP1 ZNF2 ZBTB7B ILVBL ENSG00000268564 AKAP8 BRD4 ZNF333 TPM4 AKAP8L DDX39A OR1I1 ADGRL1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around AKAP8 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the AKAP8 gene promoter:

Genomic Locations for AKAP8 Gene

Genomic Locations for AKAP8 Gene
chr19:15,353,385-15,379,828
(GRCh38/hg38)
Size:
26,444 bases
Orientation:
Minus strand
chr19:15,464,196-15,490,612
(GRCh37/hg19)

Genomic View for AKAP8 Gene

Genes around AKAP8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AKAP8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AKAP8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AKAP8 Gene

Proteins for AKAP8 Gene

  • Protein details for AKAP8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43823-AKAP8_HUMAN
    Recommended name:
    A-kinase anchor protein 8
    Protein Accession:
    O43823

    Protein attributes for AKAP8 Gene

    Size:
    692 amino acids
    Molecular mass:
    76108 Da
    Quaternary structure:
    • Binds to the PKA RII-alpha regulatory subunit PRKAR2A (phosphorylated at Thr-54) during mitosis (PubMed:9473338, PubMed:10601332, PubMed:10764601, PubMed:11591814). Interacts (via C-terminus) with FIGN (By similarity). Interacts with NCAPD2, CCND1, MCM2, RPS6KA1, PDE4A (PubMed:10601332, PubMed:11964380, PubMed:11591814, PubMed:12740381, PubMed:14641107, PubMed:15470020, PubMed:22130794). Interacts with CCND3, CCNE1, DDX5, CASP3. Interacts with NFKB1; detetcted in the cytoplasm. Interacts with MYCBP; MYCBP is translocated to the nucleus and the interaction prevents the association of the PKA catalytic subunit leading to suppression of PKA activity (By similarity). Interacts with DPY30; mediating AKAP8 association with at least the MLL4/WBP7 HMT complex (PubMed:23995757). Interacts with HDAC3; increased during mitosis (PubMed:16980585). Interacts with GJA1; in the nucleus and in the nuclear membrane; the nuclear association increases with progress of cell cycle G1, S and G2 phase and decreases in M phase (PubMed:26880274).

neXtProt entry for AKAP8 Gene

Post-translational modifications for AKAP8 Gene

  • Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes.

Other Protein References for AKAP8 Gene

No data available for DME Specific Peptides for AKAP8 Gene

Domains & Families for AKAP8 Gene

Gene Families for AKAP8 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for AKAP8 Gene

Suggested Antigen Peptide Sequences for AKAP8 Gene

Graphical View of Domain Structure for InterPro Entry

O43823

UniProtKB/Swiss-Prot:

AKAP8_HUMAN :
  • Belongs to the AKAP95 family.
Family:
  • Belongs to the AKAP95 family.
genes like me logo Genes that share domains with AKAP8: view

Function for AKAP8 Gene

Molecular function for AKAP8 Gene

GENATLAS Biochemistry:
A kinase (PRKA regulatory subunit RIIA) anchor protein 8,located in the nucleus in quiescent fibroblasts,excluding RIIA,suggesting that the interaction is cell cycle dependent,homolog to rat AKAP95
UniProtKB/Swiss-Prot Function:
Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at Ser-10 of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 Lys-4 methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).

Phenotypes From GWAS Catalog for AKAP8 Gene

Gene Ontology (GO) - Molecular Function for AKAP8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003690 double-stranded DNA binding IEA --
GO:0003723 RNA binding IDA,HDA 22658674
GO:0005515 protein binding IPI 9473338
GO:0008270 zinc ion binding IEA --
genes like me logo Genes that share ontologies with AKAP8: view
genes like me logo Genes that share phenotypes with AKAP8: view

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for AKAP8 Gene

Localization for AKAP8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AKAP8 Gene

Nucleus. Nucleus matrix. Nucleus, nucleolus. Cytoplasm. Note=Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Localized to the nucleolus in interphase. Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase. {ECO:0000269 PubMed:10601332, ECO:0000269 PubMed:26683827, ECO:0000269 PubMed:26880274}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for AKAP8 gene
Compartment Confidence
nucleus 5
mitochondrion 2
cytosol 2
golgi apparatus 2

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for AKAP8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000793 condensed chromosome IEA --
GO:0001939 female pronucleus IEA --
GO:0005634 nucleus IEA,TAS 9473338
GO:0005654 nucleoplasm IDA --
GO:0005730 nucleolus IEA --
genes like me logo Genes that share ontologies with AKAP8: view

Pathways & Interactions for AKAP8 Gene

genes like me logo Genes that share pathways with AKAP8: view

Pathways by source for AKAP8 Gene

Gene Ontology (GO) - Biological Process for AKAP8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000278 mitotic cell cycle TAS 9473338
GO:0002376 immune system process IEA --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006810 transport IEA --
genes like me logo Genes that share ontologies with AKAP8: view

No data available for SIGNOR curated interactions for AKAP8 Gene

Drugs & Compounds for AKAP8 Gene

(4) Drugs for AKAP8 Gene - From: ApexBio, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
cyclic amp Experimental Pharma 0
PKA inhibitor fragment (6-22) amide Pharma Potent protein kinase A inhibitor 0

(1) ApexBio Compounds for AKAP8 Gene

Compound Action Cas Number
PKA inhibitor fragment (6-22) amide 121932-06-7
genes like me logo Genes that share compounds with AKAP8: view

Drug Products

Transcripts for AKAP8 Gene

Unigene Clusters for AKAP8 Gene

A kinase (PRKA) anchor protein 8:
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for AKAP8 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
SP1: -
SP2: - - -
SP3: - - - - -
SP4:

Relevant External Links for AKAP8 Gene

GeneLoc Exon Structure for
AKAP8
ECgene alternative splicing isoforms for
AKAP8

Expression for AKAP8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for AKAP8 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for AKAP8 Gene

This gene is overexpressed in Adrenal (19.4) and Peripheral blood mononuclear cells (10.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for AKAP8 Gene



Protein tissue co-expression partners for AKAP8 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of AKAP8 Gene:

AKAP8

SOURCE GeneReport for Unigene cluster for AKAP8 Gene:

Hs.594496

mRNA Expression by UniProt/SwissProt for AKAP8 Gene:

O43823-AKAP8_HUMAN
Tissue specificity: Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.

Evidence on tissue expression from TISSUES for AKAP8 Gene

  • Liver(4.3)
  • Nervous system(4.3)
genes like me logo Genes that share expression patterns with AKAP8: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for AKAP8 Gene

Orthologs for AKAP8 Gene

This gene was present in the common ancestor of chordates.

Orthologs for AKAP8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia AKAP8 33 34
  • 99.08 (n)
cow
(Bos Taurus)
Mammalia AKAP8 33 34
  • 85.46 (n)
dog
(Canis familiaris)
Mammalia AKAP8 33 34
  • 83.58 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 83 (a)
OneToMany
-- 34
  • 53 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Akap8 33
  • 81.65 (n)
mouse
(Mus musculus)
Mammalia Akap8 33 16 34
  • 81.54 (n)
oppossum
(Monodelphis domestica)
Mammalia AKAP8 34
  • 61 (a)
OneToOne
chicken
(Gallus gallus)
Aves AKAP8 33
  • 67.15 (n)
lizard
(Anolis carolinensis)
Reptilia AKAP8 34
  • 54 (a)
OneToOne
Species where no ortholog for AKAP8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for AKAP8 Gene

ENSEMBL:
Gene Tree for AKAP8 (if available)
TreeFam:
Gene Tree for AKAP8 (if available)

Paralogs for AKAP8 Gene

Paralogs for AKAP8 Gene

(1) SIMAP similar genes for AKAP8 Gene using alignment to 4 proteins:

Pseudogenes.org Pseudogenes for AKAP8 Gene

genes like me logo Genes that share paralogs with AKAP8: view

Variants for AKAP8 Gene

Sequence variations from dbSNP and Humsavar for AKAP8 Gene

SNP ID Clin Chr 19 pos Variation AA Info Type
rs754301101 likely-benign, Long QT syndrome 15,372,925(-) C/A/G/T coding_sequence_variant, missense_variant, non_coding_transcript_variant
VAR_036534 A breast cancer sample p.Gln664His
rs1000143922 -- 15,355,948(-) C/A intron_variant
rs1000203417 -- 15,353,848(-) C/A 3_prime_UTR_variant, non_coding_transcript_variant
rs1000207351 -- 15,380,443(-) G/T upstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for AKAP8 Gene

Variant ID Type Subtype PubMed ID
nsv1064684 CNV gain 25217958
nsv833765 CNV loss 17160897
nsv953986 CNV deletion 24416366
nsv954995 CNV deletion 24416366

Variation tolerance for AKAP8 Gene

Residual Variation Intolerance Score: 82.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.19; 51.99% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for AKAP8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
AKAP8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for AKAP8 Gene

Disorders for AKAP8 Gene

Additional Disease Information for AKAP8

No disorders were found for AKAP8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for AKAP8 Gene

Publications for AKAP8 Gene

  1. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. (PMID: 9473338) Eide T … Taskén K (Experimental cell research 1998) 2 3 4 22 58
  2. A-kinase anchoring in dendritic cells is required for antigen presentation. (PMID: 19277197) Schillace RV … Carr DW (PloS one 2009) 3 4 22 58
  3. A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. (PMID: 16227597) Kamada S … Hunter T (Molecular and cellular biology 2005) 3 4 22 58
  4. A-kinase anchoring proteins interact with phosphodiesterases in T lymphocyte cell lines. (PMID: 15470020) Asirvatham AL … Carr DW (Journal of immunology (Baltimore, Md. : 1950) 2004) 3 4 22 58
  5. A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95. (PMID: 14641107) Arsenijevic T … Pirson I (The Biochemical journal 2004) 3 4 22 58

Products for AKAP8 Gene

Sources for AKAP8 Gene

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