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Aliases & Descriptions for AKAP12
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases AKAP250 1 , 2 , 3 DKFZp686M0430 2 DKFZp686O0331 2 FLJ20945 2 FLJ97621 2 Gravin 3 OTTHUMP00000017426 2
Descriptions A kinase (PRKA) anchor protein (gravin) 12 1 , 2 A kinase (PRKA) anchor protein 12 2 A kinase anchor protein 12 1 A-kinase anchor protein 250 kDa 3 A-kinase anchor protein, 250kDa 2 AKAP 250 3 Myasthenia gravis autoantigen 3 kinase scaffold protein gravin 2 myasthenia gravis autoantigen gravin 2
Search outside databases for aliases for AKAP12 genePrevious GC identifers: GC06P151122 GC06P151456 GC06P151592
Summaries for AKAP12 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for AKAP12 : The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have thecommon function of binding to the regulatory subunit of protein kinase A (PKA) and confining theholoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family.The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcomacells. It associates with protein kinases A and C and phosphatase, and serves as a scaffoldprotein in signal transduction. This protein and RII PKA colocalize at the cell periphery. Thisprotein is a cell growth-related protein. Antibodies to this protein can be produced by patientswith myasthenia gravis. Alternative splicing of this gene results in two transcript variantsencoding different isoforms. [provided by RefSeq] UniProtKB/Swiss-Prot: AKA12_HUMAN, Q02952 Function : Anchoring protein that mediates the subcellular compartmentation of protein kinase A(PKA) and protein kinase C (PKC)
Gene Wiki entry for AKAP12
Genomic Location for AKAP12
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the AKAP12 gene Entrez Gene cytogenetic band: 6q24-q25 Ensembl cytogenetic band: 6q25.1 HGNC cytogenetic band: 6q24-q25 AKAP12 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 6 GeneLoc Exon Structure
GeneLoc location for GC06P151653:
(about GC identifiers )
Start:
151,603,202 bp from pter
End:
151,719,602 bp from pter
Size:
116,401 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000006.10 NT_025741.14 Proteins for AKAP12
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: AKA12_HUMAN, Q02952 (See
protein sequence )Recommended Name: A-kinase anchor protein 12 Size : 1782 amino acids; 191483 Da
Subunit : Binds to dimeric RII-alpha regulatory subunit of PKC
Subcellular location : Cytoplasm, cell cortex (Probable). Cytoplasm, cytoskeleton (Probable).Note=May be part of the cortical cytoskeleton
Secondary accessions : O00310 O00498 Q4LE68 Q5SZ80 Q5TGN1 Q68D82 Q99970Alternative splicing : 3 isoforms : Q02952-1 Q02952-2 Q02952-3
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR1
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (2 alternative transcripts):
NP_005091.2 NP_653080.1 ENSEMBL proteins: ENSP00000253332 ENSP00000384537 ENSP00000346702 ENSP00000352794 ENSP00000376073 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 4 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for AKAP12: Assays for AKAP12:
Protein
Domains/ Families for AKAP12(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q02952 ProtoNet protein and cluster: Q02952
1 Blocks protein family : IPB001573 Protein kinase A anchoring domain UniProtKB/Swiss-Prot: AKA12_HUMAN, Q02952 Domain : Polybasic regions located between residues 266 and 557 are involved in binding PKCSimilarity : Contains 3 AKAP domains
Gene Function for AKAP12
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
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Millipore ,
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siRNAs from
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SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_005100 Applied Biosystems Silencer ® siRNAs for AKAP12 Sigma-Aldrich siRNA for AKAP12 Sigma-Aldrich shRNA Panels and shRNA for AKAP12 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_005100                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_005100                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_005100  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_005100 UniProtKB/Swiss-Prot: AKA12_HUMAN, Q02952 Function : Anchoring protein that mediates the subcellular compartmentation of protein kinase A(PKA) and protein kinase C (PKC) Induction : Activated by lysophosphatidylcholine (lysoPC)
Genatlas biochemistry entry for AKAP12 :A kinase (PRKA regulatory subunits PRKC and RIIA) anchor protein 12,expressed in endothelial cellsand several adherent cells
1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for Akap12) :1 Gene Ontology (GO) molecular function term (links to tree view) :
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Pathways & Interactions for AKAP12
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for AKAP12 (Your Favorite Gene powered by Ingenuity) Gene Network CentralTM Interacting Genes and Proteins Network for AKAP12 5/15 Interacting proteins for AKAP12 (Q02952 2 ENSP00000253332 3 ) via UniProtKB, MINT, and/or STRING (see all 15
)About this table 3 Gene Ontology (GO) biological process terms (links to tree view) :
About this table
Drugs & Compounds for AKAP12 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for AKAP12 1 Novoseek chemical compound relationship for AKAP12 gene
Compound
Score
Articles
PubMed IDs for Articles with Shared Sentences (# sentences)
tyrosine
1.18
1
14715081 (1)
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Transcripts for AKAP12(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
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OriGene ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_005100 Sigma-Aldrich siRNA for AKAP12 Sigma-Aldrich shRNA Panels and shRNA for AKAP12 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_005100 NM_144497
REFSEQ mRNAs for AKAP12 gene (2 alternative transcripts): NM_005100.3 NM_144497.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_005100 NM_144497
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_005100                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_005100                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_005100  
Additional cDNA sequence: AB003476.1 AB210003.1 AF001504.1 AF086250.1 AK024598.1 AK126161.1 AK307578.1 AK307673.1 AK307739.1 BC000188.1 BC015830.1 BC022814.1 BC033829.1 BC047728.1 BC070151.1 BC117679.1 BC117680.1 CR619037.1 CR749527.1 CR936604.1 M96322.1 U81607.1
14 DOTS entries : DT.95170629 DT.100823862 DT.97843539 DT.100823860 DT.87016407 DT.75106825 DT.91650893 DT.75138132 DT.100031427 DT.40132830 DT.100680885 DT.97800929 DT.121362334 DT.95170641
24/362 AceView cDNA sequences (see all 362
):BM697257 AI656759 AA570647 CB158171 BM996222 BM679663 AB003476 BQ919238 BQ772759 AA699718 BQ432119 NM_144497 CR619037 AL040086 BQ221404 BM721250 CB160154 BU735937 BM729126 BX492672 CA390252 BG112901 BF339175 AI078016
highest scoring ESTs for AKAP12 :U81607 AA010248 AA010249 AA024796 AA032141 AA092037 AA101138 AA146779 AA148133 AA470106
Unigene Cluster for AKAP12: A kinase (PRKA) anchor protein 12 Hs.371240 [show with all ESTs ] Unigene Representative Sequence: NM_005100 GeneLoc Exon Structure 5/7 Alternative Splicing Database (ASD) splice patterns (SP) for AKAP12 (see all 7
) ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8a · 8b SP1 :   -           -   -   -   -             SP2 :             -   -   -   -             SP3 :                               SP4 :             -   -   -   -   -   -   -       SP5 :                   -            
About this scheme ECgene alternative splicing isoforms for AKAP12 5 Ensembl transcripts including schematic representations : ENST00000253332
ENST00000402676
ENST00000354675
ENST00000359755
ENST00000392241
Expression for AKAP12
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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AKAP12 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for AKAP12 1 / 2 / 3
11 probe-sets matching AKAP12 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: AGTTGCCTCTSOURCE GeneReport for Unigene cluster: Hs.371240 Expression variation in blood from EXPOLDB for AKAP12
UniProtKB/Swiss-Prot: AKA12_HUMAN, Q02952 Tissue specificity : Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but notin platelets, leukocytes, monocytic cell lines or peripherical blood cells
Orthologs for AKAP12
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for AKAP12 gene from 5/8 species (see all 8
)
About this table Species with no ortholog for AKAP12 ENSEMBL Gene Tree for AKAP12 Paralogs for AKAP12 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for AKAP12 gene TCOF1 2 NOLC1 2
SNPs/Variants for AKAP12 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for AKAP12 (up to first 250kb)
Disorders & Mutations for AKAP12
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
Novoseek , PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 604698 UniProtKB/Swiss-Prot: AKA12_HUMAN, Q02952
Antibodies to the C-terminal of gravin can be produced by patients with myasthenia gravis(MG)
2 Novoseek disease relationships for AKAP12 gene
About this table 1 PharmGKB disease relationship for AKAP12 gene About this table
Medical News for AKAP12 (Possibly Related Articles in
Doctor's Guide )
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Publications for AKAP12 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/44 PubMed articles for AKAP12 gene (see all 44
): Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. (PubMed id 9000000) 2, 3, 4 Nauert J.B.... Scott J.D. (1997) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983) 3, 4 Olsen J.V....Mann M. (2006) Changes of gene expression by lysophosphatidylcholine in vascular endothelial cells: 12 up-regulated distinct genes including 5 cell growth-related, 3 thrombosis-related, and 4 others. (PubMed id 9604001) 3, 4 Sato N.... Miyata T. (1998) Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera. (PubMed id 1522245) 3, 4 Gordon T.... Ginsberg M.H. (1992) Hypermethylation of the AKAP12 promoter is a biomarker of Barrett's-associated esophageal neoplastic progression. (PubMed id 18199717) 1, 3 Jin Z....Meltzer S.J. (2008) Multiple promoters direct expression of three AKAP12 isoforms with distinct subcellular and tissue distribution profiles. (PubMed id 15496411) 1, 4 Streb J.W.... Miano J.M. (2004) G-protein-coupled receptor-associated A-kinase anchoring proteins AKAP5 and AKAP12: differential trafficking and distribution. (PubMed id 18950703) 1, 3 Chen M.H. and Malbon C.C. (2009) A-kinase anchoring protein 12 regulates the completion of cytokinesis. (PubMed id 18554502) 1, 3 Choi M.C....Bang Y.J. (2008) AKAP12 in astrocytes induces barrier functions in human endothelial cells through protein kinase Czeta. (PubMed id 18397319) 1, 3 Choi Y.K. and Kim K.W. (2008) AKAP12 regulates human blood-retinal barrier formation by downregulation of hypoxia-inducible factor-1alpha. (PubMed id 17442832) 1, 3 Choi Y.K....Kim K.W. (2007)
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Genome Databases showing AKAP12
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
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Other Databases showing AKAP12
(According to HUGE )
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--
Specialized Databases showing AKAP12 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for AKAP12 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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