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Aliases for AJUBA Gene

Aliases for AJUBA Gene

  • Ajuba LIM Protein 2 3 5
  • JUB 3 4
  • Jub, Ajuba Homolog (Xenopus Laevis) 2
  • LIM Domain-Containing Protein Ajuba 3
  • Jub, Ajuba Homolog 3

External Ids for AJUBA Gene

Previous HGNC Symbols for AJUBA Gene

  • JUB

Previous GeneCards Identifiers for AJUBA Gene

  • GC14M023441

Summaries for AJUBA Gene

GeneCards Summary for AJUBA Gene

AJUBA (Ajuba LIM Protein) is a Protein Coding gene. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and E-cadherin signaling in keratinocytes. GO annotations related to this gene include chromatin binding and actin filament binding. An important paralog of this gene is LIMD1.

UniProtKB/Swiss-Prot for AJUBA Gene

  • Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.

Gene Wiki entry for AJUBA Gene

Additional gene information for AJUBA Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AJUBA Gene

Genomics for AJUBA Gene

Regulatory Elements for AJUBA Gene

Enhancers for AJUBA Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH14H022976 1.9 FANTOM5 ENCODE dbSUPER 9.7 +2.7 2737 7 MLX ZFP64 FEZF1 DMAP1 YBX1 YY1 SLC30A9 ZNF143 ZNF548 SP3 ACIN1 NGDN PRMT5 RBM23 C14orf93 HAUS4 MMP14 LOC102724814 PABPN1 SLC22A17
GH14H022927 1.4 ENCODE dbSUPER 12.9 +53.0 53035 3 MLX DMAP1 YY1 SLC30A9 ZNF143 ZNF548 SP3 NFYC MEF2D SSRP1 NGDN HAUS4 PRMT5 ACIN1 SLC7A8 PABPN1 SLC22A17 LOC102724814 BCL2L2-PABPN1 PPP1R3E
GH14H023067 1.6 Ensembl ENCODE dbSUPER 10.8 -87.8 -87821 5 HDGF PKNOX1 MLX ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 PSMB11 PSMB5 C14orf119 ACIN1 C14orf93 CEBPE AJUBA HAUS4 RNF212B SLC7A8
GH14H023060 1.2 ENCODE dbSUPER 10.7 -80.1 -80129 4 PKNOX1 NFIB SIN3A KLF17 DNMT3B ZNF2 ZBTB40 RFX5 YY1 GLIS2 PSMB11 PSMB5 ACIN1 C14orf93 C14orf119 AJUBA HAUS4 RNF212B SLC7A8 CDH24
GH14H022955 1.1 ENCODE 11.5 +26.0 26038 2 HDGF PKNOX1 MLX ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9 HAUS4 ACIN1 C14orf93 C14orf119 RBM23 PRMT5 AJUBA PSMB5 PSMB11 CDH24
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around AJUBA on UCSC Golden Path with GeneCards custom track

Promoters for AJUBA Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000066671 842 3201 MLX ZFP64 FEZF1 DMAP1 YBX1 YY1 SLC30A9 ZNF143 ZNF548 SP3

Genomic Location for AJUBA Gene

Chromosome:
14
Start:
22,971,174 bp from pter
End:
22,982,642 bp from pter
Size:
11,469 bases
Orientation:
Minus strand

Genomic View for AJUBA Gene

Genes around AJUBA on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AJUBA Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AJUBA Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AJUBA Gene

Proteins for AJUBA Gene

  • Protein details for AJUBA Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96IF1-AJUBA_HUMAN
    Recommended name:
    LIM domain-containing protein ajuba
    Protein Accession:
    Q96IF1
    Secondary Accessions:
    • A8MX18
    • D3DS37

    Protein attributes for AJUBA Gene

    Size:
    538 amino acids
    Molecular mass:
    56934 Da
    Quaternary structure:
    • Interacts with GRB2, PIP5K1B and SLC1A2 (By similarity). Interacts with AURKA; the interaction occurs during mitosis and both proteins are phosphorylated as they form a complex. Interacts with CTNNA1 and with F-actin. Interacts with LATS2; the interaction occurs during mitosis and the complex regulates organization of the spindle apparatus through recruitment of TUBG to the centrosome. Forms a complex with SQSTM1, PRKCZ and TRAF6. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly (via the LIM domains) with GFI1; the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes, and is independent of the GFI1 SNAG domain. Interacts with HDAC1, HDAC2 and HDAC3. Interacts with SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain) (By similarity). Interacts with EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with isoform 1 and isoform 3 of VHL. Interacts (via LIM domains) with SNAI1 (via SNAG domain).
    Miscellaneous:
    • Ajuba means curiosity in Urdu, an Indian dialect.

    Alternative splice isoforms for AJUBA Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for AJUBA Gene

Post-translational modifications for AJUBA Gene

  • Phosphorylated by LATS2 during mitosis. Phosphorylated by AURKA.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for AJUBA (JUB)
  • Abcam antibodies for AJUBA

No data available for DME Specific Peptides for AJUBA Gene

Domains & Families for AJUBA Gene

Gene Families for AJUBA Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted intracellular proteins

Protein Domains for AJUBA Gene

InterPro:
Blocks:
ProtoNet:

Graphical View of Domain Structure for InterPro Entry

Q96IF1

UniProtKB/Swiss-Prot:

AJUBA_HUMAN :
  • LIM region interacts with CTNNA1. The preLIM region binds directly actin filaments.
  • Belongs to the zyxin/ajuba family.
Domain:
  • LIM region interacts with CTNNA1. The preLIM region binds directly actin filaments.
  • LIM-2 and LIM-3 domains mediate the interaction with the N-terminal region of AURKA. The association between LATS2 and AJUBA required the second LIM domain of AJUBA.
Family:
  • Belongs to the zyxin/ajuba family.
genes like me logo Genes that share domains with AJUBA: view

Function for AJUBA Gene

Molecular function for AJUBA Gene

UniProtKB/Swiss-Prot Function:
Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.

Gene Ontology (GO) - Molecular Function for AJUBA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding IEA --
GO:0003714 transcription corepressor activity IDA 18805794
GO:0005515 protein binding IPI 15778465
GO:0045294 alpha-catenin binding IDA 12417594
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with AJUBA: view
genes like me logo Genes that share phenotypes with AJUBA: view

Animal Models for AJUBA Gene

MGI Knock Outs for AJUBA:

Animal Model Products

CRISPR Products

miRNA for AJUBA Gene

miRTarBase miRNAs that target AJUBA

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for AJUBA
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for AJUBA Gene

Localization for AJUBA Gene

Subcellular locations from UniProtKB/Swiss-Prot for AJUBA Gene

Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, P-body. Note=Shuttles between the cytoplasm and the nucleus. Localizes on centrosomes during G2-M phase. Preferentially co- localizes with cadherin-adhesive complexes at sites of cell-cell contacts. Colocalizes with GFI1 in the nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for AJUBA gene
Compartment Confidence
plasma membrane 5
cytoskeleton 5
nucleus 5
cytosol 5
golgi apparatus 5

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (2)
  • Nucleus (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for AJUBA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000932 P-body IDA 20616046
GO:0005634 nucleus IEA,IDA 18805794
GO:0005667 transcription factor complex IBA --
GO:0005737 cytoplasm IEA --
GO:0005794 Golgi apparatus IDA --
genes like me logo Genes that share ontologies with AJUBA: view

Pathways & Interactions for AJUBA Gene

genes like me logo Genes that share pathways with AJUBA: view

Gene Ontology (GO) - Biological Process for AJUBA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000086 G2/M transition of mitotic cell cycle TAS --
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0001666 response to hypoxia IDA 22286099
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IBA --
genes like me logo Genes that share ontologies with AJUBA: view

No data available for SIGNOR curated interactions for AJUBA Gene

Drugs & Compounds for AJUBA Gene

No Compound Related Data Available

Transcripts for AJUBA Gene

Unigene Clusters for AJUBA Gene

Ajuba LIM protein:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for AJUBA
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

Alternative Splicing Database (ASD) splice patterns (SP) for AJUBA Gene

No ASD Table

Relevant External Links for AJUBA Gene

GeneLoc Exon Structure for
AJUBA
ECgene alternative splicing isoforms for
AJUBA

Expression for AJUBA Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for AJUBA Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for AJUBA Gene

This gene is overexpressed in Liver (x4.2).

Protein differential expression in normal tissues from HIPED for AJUBA Gene

This gene is overexpressed in Heart (26.4), Fetal Liver (22.7), Placenta (10.6), and Fetal heart (9.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for AJUBA Gene



Protein tissue co-expression partners for AJUBA Gene

NURSA nuclear receptor signaling pathways regulating expression of AJUBA Gene:

AJUBA

SOURCE GeneReport for Unigene cluster for AJUBA Gene:

Hs.655832

Evidence on tissue expression from TISSUES for AJUBA Gene

  • Liver(4.5)
genes like me logo Genes that share expression patterns with AJUBA: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for AJUBA Gene

Orthologs for AJUBA Gene

This gene was present in the common ancestor of animals.

Orthologs for AJUBA Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia AJUBA 34
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia AJUBA 33 34
  • 91.95 (n)
cow
(Bos Taurus)
Mammalia AJUBA 33 34
  • 91.64 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia AJUBA 34
  • 91 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ajuba 33 16 34
  • 88.27 (n)
rat
(Rattus norvegicus)
Mammalia Ajuba 33
  • 88.17 (n)
zebrafish
(Danio rerio)
Actinopterygii ajuba 34
  • 35 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta jub 34
  • 25 (a)
OneToMany
Species where no ortholog for AJUBA was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for AJUBA Gene

ENSEMBL:
Gene Tree for AJUBA (if available)
TreeFam:
Gene Tree for AJUBA (if available)

Paralogs for AJUBA Gene

Paralogs for AJUBA Gene

(8) SIMAP similar genes for AJUBA Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with AJUBA: view

Variants for AJUBA Gene

Sequence variations from dbSNP and Humsavar for AJUBA Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type
rs1000329378 -- 22,973,266(+) GGTTT(C/G)TCTTC utr-variant-3-prime
rs1000331319 -- 22,979,519(+) GAGGG(C/T)ACAGT intron-variant
rs1000807294 -- 22,979,914(+) CCCTG(A/T)CATTC intron-variant
rs1000870961 -- 22,974,475(+) TCTCC(A/G)TAGGC intron-variant
rs1001070161 -- 22,980,930(+) GAGCA(A/G)GTTGG intron-variant, upstream-variant-2KB

Variation tolerance for AJUBA Gene

Residual Variation Intolerance Score: 64.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.40; 42.39% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for AJUBA Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
AJUBA

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for AJUBA Gene

Disorders for AJUBA Gene

Relevant External Links for AJUBA

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
AJUBA

No disorders were found for AJUBA Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for AJUBA Gene

Publications for AJUBA Gene

  1. The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity. (PMID: 22286099) Foxler DE … Sharp TV (Nature cell biology 2012) 3 4 60
  2. LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. (PMID: 20616046) James V … Sharp TV (Proceedings of the National Academy of Sciences of the United States of America 2010) 3 4 60
  3. Ajuba LIM proteins are negative regulators of the Hippo signaling pathway. (PMID: 20303269) Das Thakur M … Longmore GD (Current biology : CB 2010) 3 4 60
  4. Ajuba functions as a histone deacetylase-dependent co-repressor for autoregulation of the growth factor-independent-1 transcription factor. (PMID: 18805794) Montoya-Durango DE … Grimes HL (The Journal of biological chemistry 2008) 3 4 60
  5. Ajuba LIM proteins are snail/slug corepressors required for neural crest development in Xenopus. (PMID: 18331720) Langer EM … Longmore GD (Developmental cell 2008) 3 4 60

Products for AJUBA Gene

Sources for AJUBA Gene

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