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AIM2 Gene

protein-coding GIFtS: 56

GC01M157298

absent in melanoma 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
OTTHUMP00000035296 ^²
PYHIN4 ^¹, ²

Descriptions
absent in melanoma 2 ^², ³

External Ids
HGNC: 357¹
Entrez Gene: 9447²
UniProtKB: O14862³
Ensembl: ENSG00000163568⁷

Search outside databases for aliases for AIM2 gene

Previous GC identifers: GC01M156824 GC01M154764 GC01M155810 GC01M156249 GC01M155845

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for AIM2:

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and

may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq]

UniProtKB/Swiss-Prot: AIM2_HUMAN, O14862

Function: Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity

Gene Wiki entry for AIM2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the AIM2 gene

Entrez Gene cytogenetic band: 1q22 Ensembl cytogenetic band: 1q23.2 HGNC cytogenetic band: 1q22

AIM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01M157298: (about GC identifiers)

Start:	157,298,665 bp from pter
End:	157,313,271 bp from pter
Size:	14,607 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000001.9 NT_004487.18

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: AIM2_HUMAN, O14862 (See protein sequence)

Recommended Name: Interferon-inducible protein AIM2

Size: 343 amino acids; 38954 Da

Subunit: Homodimer

Subcellular location: Nucleus

Sequence caution: Sequence=AAH10940.1; Type=Frameshift; Positions=340;

Secondary accessions: A8K7M7 Q5T3V9 Q96FG9

REFSEQ proteins: NP_004824.1

ENSEMBL proteins:

ENSP00000357111 ENSP00000357112

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
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	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (AIM2)
	Human Recombinant Proteins from Abnova (AIM2)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--

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Antibodies for AIM2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for AIM2
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (AIM2), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (AIM2)
	Novus Biologicals Antibodies for AIM2

Assays for AIM2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry O14862

ProtoNet protein and cluster: O14862

2 Blocks protein families:

IPB004020 Pyrin domain

IPB004021 HIN-200/IF120x domain

UniProtKB/Swiss-Prot: AIM2_HUMAN, O14862

Similarity: Belongs to the HIN-200 family

Similarity: Contains 1 DAPIN domain

Similarity: Contains 1 HIN-200 domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (AIM2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004833

Applied Biosystems Silencer® siRNAs for AIM2

Sigma-Aldrich siRNA for AIM2

Sigma-Aldrich shRNA for AIM2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for AIM2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_004833
Myc/DDK tagged cDNA clone in CMV expression vector: NM_004833
untagged cDNA clone in CMV expression vector: NM_004833

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_004833

UniProtKB/Swiss-Prot: AIM2_HUMAN, O14862

Function: Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity

Induction: By interferon gamma

Genatlas biochemistry entry for AIM2:

interferon gamma inducible gene,homolog to the tightly linked genes IFI16,MNDA,expressed in

spleen,small intestine,peripheral blood leukocytes,39.5kDa,absent in melanoma 2

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Interacting protein for AIM2 (ENSP00000357111³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
SULT2B1	ENSP00000201586³	STRING (score=.737)

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2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006955	immune response	TAS	9242382
GO:0045786	negative regulation of cell cycle	IEA	--

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for AIM2
1 Novoseek chemical compound relationship for AIM2 gene

Compound	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
mnda	97.78	7	9242382 (2), 10828447 (1), 11215814 (1), 17911419 (1) (see all 6)

About this table

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (AIM2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004833

Sigma-Aldrich siRNA for AIM2

Sigma-Aldrich shRNA for AIM2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_004833

REFSEQ mRNAs for AIM2 gene:

NM_004833.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_004833

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004833
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004833
                                 untagged cDNA clone in CMV expression vector: NM_004833

Additional cDNA sequence:

AF024714.1 AK292042.1 BC010940.1 U52053.1

7 DOTS entries:

DT.310623 DT.100711389 DT.97770207 DT.100649691 DT.102831425 DT.310624 DT.92415868

24/34 AceView cDNA sequences (see all 34 ):

CB053629 AA458912 BM717965 AF024714 NM_004833 CD703806 BU943059 AW294096

AA555091 AA459132 AA568884 CB151016 AA215558 CD107288 AA767288 BU599245

BC010940 U52053 CA432643 CF137554 CD366430 BP339102 BI912227 BF244586

highest scoring ESTs for AIM2:

AF024714 AA459132 BC010940 BI912227 BU599245 BU934160 BU943059 CB053630 AA555091 AA568884

Unigene Cluster for AIM2:

Absent in melanoma 2

Hs.281898 [show with all ESTs]

Unigene Representative Sequence: AK292042

GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:

ENST00000368129 ENST00000368130

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

AIM2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for AIM2

¹ / ² / ³

3 probe-sets matching AIM2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
34439_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	AF024714	1.00	1.00	1.00	1
206513_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_004833	0.60	1.00	0.82	1
206513_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks AIM2 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: CTACAGTGGC

SOURCE GeneReport for Unigene cluster: Hs.281898
Expression variation in blood from EXPOLDB for AIM2
UniProtKB/Swiss-Prot: AIM2_HUMAN, O14862
Tissue specificity: Expressed in spleen, small intestine, peripheral blood leukocytes, and testis

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for AIM2 gene from 3 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	AIM2¹	--	absent in melanoma 2	98.74(n) 97.96(a)	457425 XM_513914.2 XP_513914.2
rat (Rattus norvegicus)	Aim2¹	--	absent in melanoma 2	69.11(n) 57.48(a)	304987 XM_222949.4 XP_222949.4
mouse (Mus musculus)	Aim2^{1, 5}	1⁵	absent in melanoma 2^{1, 5}	67.77(n)¹ 57.02(a)¹	383619¹ NM_001013779.1¹ NP_001013801.1¹ AC084073⁵ AK050724⁵ (see all 7)

About this table Species with no ortholog for AIM2

ENSEMBL Gene Tree for AIM2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for AIM2 gene

IFI16² PYHIN1² MNDA²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/75 NCBI SNPs in AIM2 are shown (see all 75 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 46)

AB	SNP ID	Valid	Chr 1 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs2793845^1,2	C,F,H,O	157298879(+)	`CTAAAT/GGAGAT`	1	--	ng5¹		8	EU EA WA NA	630
	rs855873^1,2	C,F,H	157314336(+)	`TCATGG/AAAACA`	1	--	ng3¹		11	EU EA WA NA	956
	rs2298803^1,2	C,F,H	157313054(+)	`CCTGAT/CCCCTG`	1	--	ut5¹ ese³		5	EA EU WA	1908
	rs2276405^1,2	C,F,H	157309820(+)	`AAACTC/TGTCTG`	1	E/K	mis¹ ese³		13	EA NA EU WA	2136
	rs16841642^1,2	C,F,H	157313422(+)	`ACTTTG/TTGACT`	1	--	ng3¹		12	NA EU EA WA	1056
	rs11265139^1,2	F,H	157313795(+)	`aactgT/Ccatgg`	1	--	ng3¹		4	EU EA WA	418
	rs2570921^1,2	H	157313487(+)	`GGTATC/TTAAAA`	1	--	ng3¹		4	EU EA WA	420
	rs4292958^1,2	H	157305077(+)	`ACTTAG/ATGGCT`	1	L/L	syn¹ ese³		4	EU EA WA	412
	rs34479821^1,2	F	157309665(-)	`CAGGAG/AGAGAA`	1	E/E	syn¹		1	NA	78
	rs855871^1,2	A,C,F,H,O	157311275(+)	`aatggA/Gaagat`	1	--	int¹		8	MN EU EA WA NA	736

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HapMap Linkage Disequilibrium images for AIM2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
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OMIM: 604578

UniProtKB/Swiss-Prot: AIM2_HUMAN, O14862

Defects in AIM2 may be a cause of microsatellite unstable colon cancers

2 Novoseek disease relationships for AIM2 gene

Disease	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
melanoma	34.13	6	10828447 (2), 15582594 (2), 9242382 (1)
tumors	0.00	3	15076139 (2), 15582594 (1)

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(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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10/18 PubMed articles for AIM2 gene (see all 18 ):

Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma. (PubMed id 9242382)^{1, 2, 3, 4} DeYoung K.L.... Trent J.M. (1997)
Biochemical and growth regulatory activities of the HIN-200 family member and putative tumor suppressor protein, AIM2. (PubMed id 15582594)^{1, 3, 4} Cresswell K.S....Johnstone R.W. (2005)
The putative tumor suppressor AIM2 is frequently affected by different genetic alterations in microsatellite unstable colon cancers. (PubMed id 17726700)^{3, 4} Woerner S.M.... Dihlmann S. (2007)
AIM2 suppresses human breast cancer cell proliferation in vitro and mammary tumor growth in a mouse model. (PubMed id 16432157)^{3, 4} Chen I.-F.... Hung M.-C. (2006)
The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)^{3, 4} Gregory S.G.... Bentley D.R. (2006)
AIM-2: a novel tumor antigen is expressed and presented by human glioma cells. (PubMed id 15076139)^{1, 3} Liu G....Ying H. (2004)
Cytoplasmic localization of the interferon-inducible protein that is encoded by the AIM2 (absent in melanoma) gene from the 200-gene family. (PubMed id 10828447)^{1, 3} Choubey D....Xin H. (2000)
AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. (PubMed id 19158676)³ Fernandes-Alnemri T....Alnemri E.S. (2009)
AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC. (PubMed id 19158675)³ Hornung V....Fitzgerald K.A. (2009)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 9447	HGNC: 357	AceView: AIM2	Ensembl:ENSG00000163568	euGenes: HUgn9447
ECgene: AIM2	H-InvDB: AIM2

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
ATLAS Chromosomes in Cancer entry for AIM2	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for AIM2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for AIM2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for AIM2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for AIM2		Antibodies for AIM2
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for AIM2		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for AIM2

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AIM2 Gene

protein-coding GIFtS: 56

Aliases &
Descriptions
for AIM2

Summaries
for AIM2

Genomic Location
for AIM2

Proteins
for AIM2

Protein Domains/
Families
for AIM2

Gene Function
for AIM2

Pathways & Interactions
for AIM2

Drugs & Compounds
for AIM2

Transcripts
for AIM2

Expression
for AIM2

Orthologs
for AIM2

Paralogs
for AIM2

SNPs/Variants
for AIM2

Disorders & Mutations
for AIM2

Medical News
for AIM2

Publications
for AIM2

Search for AIM2

Genome Databases showing AIM2

Other Databases showing AIM2

Specialized Databases showing AIM2

Licensable Technologies
for AIM2

Services
for AIM2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

AIM2 Gene

protein-coding GIFtS: 56

Aliases & Descriptionsfor AIM2

Summariesfor AIM2

Genomic Locationfor AIM2

Proteinsfor AIM2

Protein Domains/Familiesfor AIM2

Gene Functionfor AIM2

Pathways & Interactionsfor AIM2

Drugs & Compoundsfor AIM2

Transcriptsfor AIM2

Expression for AIM2

Orthologsfor AIM2

Paralogsfor AIM2

SNPs/Variantsfor AIM2

Disorders & Mutationsfor AIM2

Medical Newsfor AIM2

Publicationsfor AIM2

Search for AIM2

Genome Databases showing AIM2

Other Databases showing AIM2

Specialized Databases showing AIM2

Licensable Technologiesfor AIM2

Servicesfor AIM2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for AIM2

Summaries
for AIM2

Genomic Location
for AIM2

Proteins
for AIM2

Protein Domains/
Families
for AIM2

Gene Function
for AIM2

Pathways & Interactions
for AIM2

Drugs & Compounds
for AIM2

Transcripts
for AIM2

Expression
for AIM2

Orthologs
for AIM2

Paralogs
for AIM2

SNPs/Variants
for AIM2

Disorders & Mutations
for AIM2

Medical News
for AIM2

Publications
for AIM2

Licensable Technologies
for AIM2

Services
for AIM2