AIFM2 Gene
protein-coding GIFtS: 52
GCID: GC10M071857
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apoptosis-inducing factor, mitochondrion-associated, 2(Previous name: apoptosis-inducing factor (AIF)-like mitochondrion-associated...) (Previous symbol: AMID)
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Aliases for AIFM2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
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| Apoptosis-Inducing Factor, Mitochondrion-Associated, 21 2 | | RP11-367H5.22 | | AMID1 2 3 | | Apoptosis-Inducing Factor (AIF)-Homologous Mitochondrion-Associated Inducer OfDeath2 | | PRG31 2 3 | | Apoptosis-Inducing Factor 22 | | Apoptosis-Inducing Factor (AIF)-Like Mitochondrion-Associated Inducer Of Death1 2 | | EC 1.-.-.-3 | | P53-Responsive Gene 3 Protein2 3 | | Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of Death3 | | FLJ144971 | | Apoptosis-Inducing Factor-Like Mitochondrion-Associated Inducer Of Death3 |
Export aliases for AIFM2 gene to outside databasesPrevious GC identifers: GC10M071528 GC10M065864 |
Summaries for AIFM2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| Entrez Gene summary for AIFM2: This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute toapoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumorsuppressor protein p53 in colon cancer cells. (provided by RefSeq, Nov 2010) UniProtKB/Swiss-Prot: AIFM2_HUMAN, Q9BRQ8Function: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probableoxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in asequence-independent manner. May contribute to genotoxin-induced growth arrest Gene Wiki entry for AIFM2
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Genomic Views for AIFM2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000010.10 NC_018921.1 NT_030059.13
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the AIFM2 gene promoter: AhR Pax-5 AP-1 ATF-2 Arnt GATA-1 FOXL1 CREB FOXO4 c-Jun Other transcription factors
Search SABiosciences Chromatin IP Primers for AIFM2
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AIFM2 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 10q22.1 Ensembl cytogenetic band: 10q22.1 HGNC cytogenetic band: 10q22.2AIFM2 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 10 GeneLoc Exon Structure GeneLoc location for GC10M071857: view genomic region
(about GC identifiers)
Start:
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71,857,979 bp from pter |
End:
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71,892,690 bp from pter |
Size:
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34,712 bases |
Orientation:
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minus strand |
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Proteins for AIFM2 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: AIFM2_HUMAN, Q9BRQ8 (See
protein sequence)Recommended Name: Apoptosis-inducing factor 2 Size: 373 amino acids; 40527 Da
Cofactor: FAD. Binds 6-hydroxy-FAD non-covalently
Subcellular location: Cytoplasm. Mitochondrion outer membrane
Secondary accessions: B3KXI0 Q63Z39Alternative splicing: 2 isoforms: Q9BRQ8-1 Q9BRQ8-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for AIFM2: NX_Q9BRQ8
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9BRQ8 AIFM2 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_116186.1 NP_001185625.1 ENSEMBL proteins: ENSP00000362345 ENSP00000312370 Human Recombinant Protein Products:
Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6): About this table
AIFM2 for ontologies About GeneDecksing
AIFM2 Antibody Products: Assay Products for AIFM2: |
Protein
Domains / Families for AIFM2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
AIFM2 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9BRQ8ProtoNet protein and cluster: Q9BRQ8 2 Blocks protein families: IPB000103 Pyridine nucleotide-disulphide oxidoreductase IPB001327 Pyridine nucleotide-disulphide oxidoreductase
UniProtKB/Swiss-Prot: AIFM2_HUMAN, Q9BRQ8Similarity: Belongs to the FAD-dependent oxidoreductase family |
Function for AIFM2 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: AIFM2_HUMAN, Q9BRQ8Function: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probableoxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in asequence-independent manner. May contribute to genotoxin-induced growth arrestInduction: Expression detected at 4 hours after induction by p53/TP53. Down-regulated in a wide range of human tumorsEnzyme Number (IUBMB): EC 1.-.-.-1
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for AIFM2 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AIFM2 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): AIFM2 (NM_001198696) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for AIFM2 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AIFM2  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AIFM2 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table
AIFM2 for ontologies About GeneDecksing
Animal Models: Mouse knock-out Aifm2tm1Hbsh for AIFM2 3 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for Aifm2):
AIFM2 for phenotypes About GeneDecksing
|
Pathways & Interactions for AIFM2 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways  About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
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| 1 | Direct p53 effectors | |
1 BioSystems Pathway for AIFM2 
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for AIFM2
STRING Interaction
Network Preview (showing 3 interactants - click image to see more details)
 3 Interacting proteins for AIFM2 (Q9BRQ83 ENSP000003123704) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
AIFM2 for ontologies About GeneDecksing
|
Drugs & Compounds for AIFM2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Browse Tocris compounds for AIFM2
1 HMDB Compound for AIFM2 About this table
| Compound | Synonyms |
CAS
# | PubMed Ids |
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| FAD | 1H-Purin-6-amine flavin dinucleotide (see all 21) | 146-14-5 | -- | Search CenterWatch for drugs/clinical trials and news about AIFM2 
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Transcripts for AIFM2 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for AIFM2 gene (2 alternative transcripts): NM_032797.5 NM_001198696.1 Unigene Cluster for AIFM2: Apoptosis-inducing factor, mitochondrion-associated, 2 Hs.650680 [show with all ESTs]Unigene Representative Sequence: NM_0011986963 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000373248(uc010qjg.2) ENST00000307864(uc021psi.1 uc001jqp.2) ENST00000482166
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for AIFM2 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AIFM2 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): AIFM2 (NM_001198696) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for AIFM2 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AIFM2  |
Additional cDNA sequence: AF506757.1 AK027403.1 BC006121.1 BC023601.2 14 DOTS entries: DT.454747 DT.101982661 DT.100783285 DT.100792928 DT.100665228 DT.102829849 DT.97862695 DT.92426002 DT.92426003 DT.100783287 DT.101957929 DT.121261095 DT.121261116 DT.97842335 24/143 AceView cDNA sequences (see all 143): AK127353 BG332787 AW016581 CD634367 CB215085 CA314960 BC006121 AF506757 BU158922 AW239408 AL556311 AI922797 BC023601 BG775126 BF198007 NM_032797 BQ635940 AK027403 BU173890 CD514797 AL555902 BX283086 BU732124 CR622972 GeneLoc Exon Structure
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Expression for AIFM2 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| AIFM2 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: GCACCCGCCT
About this image See AIFM2 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for AIFM2
SOURCE GeneReport for Unigene cluster: Hs.650680 UniProtKB/Swiss-Prot: AIFM2_HUMAN, Q9BRQ8Tissue specificity: Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively.Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung,kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems tobe the major transcript in EB1 colon cancer cells SABiosciences Expression via Pathway-Focused PCR Arrays including AIFM2:
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for AIFM2 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat AIFM2 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat AIFM2 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat AIFM2 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AIFM2 |
Orthologs for AIFM2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for AIFM2 gene from 7/23 species (see all 23) About this table
ENSEMBL Gene Tree for AIFM2 (if available) TreeFam Gene Tree for AIFM2 (if available)  |
Paralogs for AIFM2 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| -- |
Genomic Variants for AIFM2 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 10 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
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HapMap Linkage Disequilibrium report for AIFM2 (71857979 - 71892690 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 1 variation for AIFM2 1 CNV: 6879  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing AIFM2 |
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Disorders
/ Diseases for AIFM2 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
AIFM2 for disorders About GeneDecksing
OMIM gene information: 605159
OMIM disorders: --
3 diseases for AIFM2: About MalaCardscolon cancer epithelial ovarian cancer ovarian cancer Human Genome Epidemiology (HuGE) Navigator: AIFM2 (3 documents) Export disorders for AIFM2 gene to outside databases
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Publications for AIFM2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for AIFM2 gene, integrated from 9 sources (see all 25): (articles sorted by number of sources associating them with AIFM2) | |  | Utopia: connect your pdf to the dynamic world of online information |
- The human apoptosis-inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity. (PubMed id 15958387)1, 2, 3, 9 Marshall K.R.... Munro A.W. (2005)
- AMID, an apoptosis-inducing factor-homologous mitochondrion- associated protein, induces caspase-independent apoptosis. (PubMed id 11980907)1, 2, 3 Wu M.... Shu H.-B. (2002)
- A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF). (PubMed id 12135761)1, 2, 3 Ohiro Y.... Horikoshi N. (2002)
- AMID is a p53-inducible gene downregulated in tumors. (PubMed id 15273740)1, 2 Wu M.... Shu H.-B. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)1, 2 Deloukas P.... Rogers J. (2004)
- Functional genomics identifies therapeutic targets for MYC-driven cancer. (PubMed id 22623531)1 Toyoshima M....Grandori C. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
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External Searches for AIFM2 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
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Genome Databases showing AIFM2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing AIFM2 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing AIFM2 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for AIFM2 | Pharmacogenomics, SNPs, Pathways |
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| | |
About This Section
| Patent Information for AIFM2 gene: Search GeneIP for patents involving AIFM2
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for AIFM2 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
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| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat AIFM2 | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing AIFM2 | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AIFM2 | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat AIFM2 | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat AIFM2 | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat AIFM2 |
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 | | AIFM2 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AIFM2 |
|  |  |  | | | ThermoFisher Antibody for AIFM2 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AIFM2 |
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