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AGR2 Gene

protein-coding   GIFtS: 59

GC07M016797
anterior gradient homolog 2 (Xenopus laevis)
(Previous name: anterior gradient 2 homolog (Xenopus laevis) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AG-2 3
AG2 1, 2, 3, 5
GOB-4 2
HAG-2 1, 2
HPC8 3
PDIA17 1, 2
XAG-2 1, 2
hAG-2 3
Descriptions
Secreted cement gland protein XAG-2 homolog 3
anterior gradient 2 homolog 2
anterior gradient 2 homolog (Xenopus laevis) 1
anterior gradient homolog 2 (Xenopus laevis) 2
protein disulfide isomerase family A, member 17 2
secreted cement gland homolog 2
External Ids
HGNC: 3281
Entrez Gene: 105512
UniProtKB: O959943
Ensembl: ENSG000001065417
Search outside databases for aliases for AGR2 gene

Previous GC identifers: GC07M016476 GC07M016575 GC07M016605

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Gene Wiki entry for AGR2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AGR2 gene  

Entrez Gene cytogenetic band: 7p21.3   Ensembl cytogenetic band:  7p21.1   HGNC cytogenetic band: 7p21.3

AGR2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M016797:     (about GC identifiers)

Start:
16,797,960 bp from pter
End:
16,811,136 bp from pter
Size:
13,177 bases
Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 16,885,092-16,898,265     
RefSeq DNA sequence:
NC_000007.12  NT_007819.16  NT_079592.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: AGR2_HUMAN, O95994 (See protein sequence)
Recommended Name: Anterior gradient protein 2 homolog precursor  
Size: 175 amino acids; 19979 Da
Subunit: Interacts with LYPD3 and alpha-dystroglycan
Subcellular location: Secreted (Probable)

REFSEQ proteins: NP_006399.1  

ENSEMBL proteins: 
ENSP00000386025 ENSP00000223274 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Anterior Gradient 2, AGR2 + AGR3)
Human Recombinant Proteins from Abnova (AGR2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
GO:0005737 cytoplasm IDA18029348
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Antibodies for AGR2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for AGR2
Browse R&D Systems for Antibodies
Antibodies from Abcam (Anterior Gradient 2, AGR2 + AGR3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (AGR2)
Novus Biologicals Antibodies for AGR2

Assays for AGR2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR012335 Thioredoxin_fold


   GeneDecks  AGR2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry O95994

ProtoNet protein and cluster: O95994

UniProtKB/Swiss-Prot: AGR2_HUMAN, O95994
Similarity: Belongs to the AGR family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (AGR2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (AGR2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006408

              Applied Biosystems Silencer® siRNAs for AGR2

              Sigma-Aldrich siRNA for AGR2  
                     Sigma-Aldrich shRNA for AGR2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for AGR2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006408
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_006408
                                 untagged cDNA clone in CMV expression vector: NM_006408 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_006408

3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Agr2):

digestive/alimentaryhematopoietic systemimmune system

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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 Gene Network CentralTM Interacting Genes and Proteins Network for  AGR2 


5/8 Interacting proteins for AGR2 (ENSP000002232743 O959942) via UniProtKB, MINT, and/or STRING (see all 8 )
InteractantInteraction Details
GeneCardExternal ID(s)
UBQLN4Q9NRR52MINT-2868528
LYPD3ENSP000002443333STRING (score=.938)
HBG1ENSP000003696063STRING (score=.899)
HMGB2ENSP000002965033STRING (score=.899)
OGG1ENSP000003065613STRING (score=.899)
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for AGR2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (AGR2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (AGR2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006408

              Sigma-Aldrich siRNA for AGR2  
                     Sigma-Aldrich shRNA for AGR2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_006408  

REFSEQ mRNAs for AGR2 gene: 

NM_006408.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_006408  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006408
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_006408
                                 untagged cDNA clone in CMV expression vector: NM_006408 

Additional cDNA sequence: 

AF007791.1 AF038451.1 AF038452.1 AF087879.1 AF088867.1 AF115926.1 AK124419.1 AK223067.1 
AK296721.1 AK312942.1 AY359009.1 BC015503.1 BT007048.1 CR610899.1 

18 DOTS entries:

DT.100810938  DT.75105686  DT.95279263  DT.95092567  DT.121112254  DT.92007777  DT.121112258  DT.97860996 
DT.99960432  DT.121112240  DT.121112251  DT.91752704  DT.95253542  DT.95279319  DT.121112245  DT.95279313 
DT.97804195  DT.121112242 

24/663 AceView cDNA sequences (see all 663 ):

BQ084526 AI249798 BM768900 BI770277 AW192135 BQ084070 BM854269 BM769244 
BM790204 BM795284 BQ083985 BM796390 BM789904 BM796396 CB144392 BM856780 
AW196067 BM791184 BM790980 BM765629 BM852535 BM828131 BX387240 AI445127 

highest scoring ESTs for AGR2:

AF038451 AA055880 AA298527 AA307795 AA313235 AA314146 AA314206 AA314372 AA315408 AA315613 

Unigene Cluster for AGR2:

Anterior gradient homolog 2 (Xenopus laevis)
Hs.530009  [show with all ESTs]
Unigene Representative Sequence: BM924878


GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for AGR2 (see all 6 )

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c
SP1:                                                        -           -           -                     
SP2:                    -                                   -           -           -                     
SP3:                                                        -                                             
SP4:                    -                                   -           -                                 
SP5:                                                                                                      

About this scheme

ECgene alternative splicing isoforms for AGR2

2 Ensembl transcripts including schematic representations:
ENST00000401412  ENST00000223274  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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AGR2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for AGR2

1 / 2 / 3

6 probe-sets matching AGR2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
38827_at2, 3 U95-A 1 1.00 1.00 0.99 0.82 AF038451 1.00 1.00 1.00 1
50849_at2, 3 U95-B 1 1.00 1.00 0.88 1.39 AI922323 0.60 1.00 0.82 1

209173_at2, 3 U133-A 1 1.00 1.00 -- -- AF088867 0.80 1.00 0.91 1

228969_at2, 3 U133-B 1 0.82 1.00 -- -- AI922323 0.60 1.00 0.82 1

209173_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

228969_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
GeneDecks  AGR2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ATTTTCTAAA

SOURCE GeneReport for Unigene cluster: Hs.530009

Expression variation in blood from EXPOLDB for AGR2

UniProtKB/Swiss-Prot: AGR2_HUMAN, O95994
Tissue specificity: Expressed strongly in trachea, lung, stomach, colon, prostate and small
intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix,
ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in
estrogen receptor (ER)-positive breast cancer cell lines

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for AGR2 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
AGR21   -- anterior gradient homolog 2 (Xenopus laevis) 90.29(n)
95.43(a)
482333  XM_539450.2  XP_539450.1 
cow
(Bos taurus)
AGR21   -- anterior gradient homolog 2 (Xenopus laevis) 92.57(n)
95.43(a)
415112  NM_001040500.1  NP_001035590.1 
rat
(Rattus norvegicus)
Agr21   -- anterior gradient homolog 2 (Xenopus laevis) 87.81(n)
91.43(a)
298961  XM_216691.4  XP_216691.1 
mouse
(Mus musculus)
Agr21, 5 125
anterior gradient 2 (Xenopus laevis)1, 5 87.81(n)1
91.43(a)1
237951  NM_011783.21  NP_035913.11 
 AB0165925  AF0442625  (see all 8)
chicken
(Gallus gallus)
AGR21   -- anterior gradient homolog 2 (Xenopus laevis) 75.49(n)
81.18(a)
420596  XM_418698.2  XP_418698.1 
About this table        Species with no ortholog for AGR2

ENSEMBL Gene Tree for AGR2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
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Paralogs for AGR2 gene
TXNDC122  AGR32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/151 NCBI SNPs in AGR2 are shown (see all 151 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 132)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 7 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs20614721,2
A,C,F,H,O16811463(+) TGGACA/GGAGTT 1 -- ng314Minor allele frequency- G:0.02NA EU 262
rs14595641,2
A,C,F,H16812671(+) tgtctG/Atgtca 1 -- ng314Minor allele frequency- A:0.18EU EA WA 420
rs80711,2
A,C,F,H16798907(-) AAACAA/TGTTTT 1 -- ut315Minor allele frequency- T:0.12MN EU EA WA 596
rs7060731,2
A,C,F,H16811206(+) CCCACT/CAGTGC 1 -- ut512Minor allele frequency- C:0.46EA 164
rs7060721,2
A,C,F,H16811188(+) GGGCAC/TACCTC 1 -- ut517Minor allele frequency- T:0.09EU EA WA NA 550
rs155041,2
A,C,F,H16798062(+) ATCAGG/TAGGGG 1 -- ut31 ese35Minor allele frequency- T:0.16MN EU EA WA 574
rs7060751,2
A,C,F,H16812879(+) cctagT/Gtgttg 1 -- ng314Minor allele frequency- G:0.45EU EA WA 418
rs126723011,2
C,F,H16812484(+) agtcaC/Ggagat 1 -- ng314Minor allele frequency- G:0.16EU EA WA 420
rs126659571,2
C,F,H16812737(+) tgaccT/Aacttt 1 -- ng314Minor allele frequency- A:0.15EU EA WA 412
--
rs126722941,2
A,C,F16812310(+) CCTTAG/TATAga 1 -- ng31 tfbs33Minor allele frequency- T:0.22NA 142
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HapMap Linkage Disequilibrium images for AGR2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 606358

2 Novoseek disease relationships for AGR2 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
breast cancer 28.60 8 16551856 (4), 9790916 (3)
tumors 0.00 7 16551856 (4), 9790916 (1)
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Genetic Association Database: AGR2
Human Genome Epidemiology Navigator: AGR2 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/27 PubMed articles for AGR2 gene (see all 27 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 10551 HGNC: 328 AceView: AGR2 Ensembl:ENSG00000106541 euGenes: HUgn10551
ECgene: AGR2 H-InvDB: AGR2
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for AGR2 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for AGR2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for AGR2 Antibodies for AGR2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for AGR2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(Anterior Gradient 2, AGR2 + AGR3)
 Antibodies (Anterior Gradient 2, AGR2 + AGR3)
 Search OriGene for AGR2
 Search Tocris compounds for AGR2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for AGR2

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