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Aliases for AGO4 Gene

Aliases for AGO4 Gene

  • Argonaute 4, RISC Catalytic Component 2 3 5
  • Argonaute RISC Catalytic Component 4 2 3 4
  • Eukaryotic Translation Initiation Factor 2C, 4 2 3
  • Argonaute 4 2 3
  • Argonaute4 3 4
  • EIF-2C 4 3 4
  • EIF2C 4 3 4
  • EIF2C4 3 4
  • HAgo4 3 4
  • Eukaryotic Translation Initiation Factor 2C 4 4
  • Protein Argonaute-4 3
  • KIAA1567 4

External Ids for AGO4 Gene

Previous HGNC Symbols for AGO4 Gene

  • EIF2C4

Previous GeneCards Identifiers for AGO4 Gene

  • GC01P035329
  • GC01P036274

Summaries for AGO4 Gene

Entrez Gene Summary for AGO4 Gene

  • This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1. [provided by RefSeq, Jul 2008]

GeneCards Summary for AGO4 Gene

AGO4 (Argonaute 4, RISC Catalytic Component) is a Protein Coding gene. Among its related pathways are RET signaling and Immune System. GO annotations related to this gene include nucleic acid binding and double-stranded RNA binding. An important paralog of this gene is AGO1.

UniProtKB/Swiss-Prot for AGO4 Gene

  • Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AGO4 Gene

Genomics for AGO4 Gene

Regulatory Elements for AGO4 Gene

Enhancers for AGO4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH01F036159 1.7 FANTOM5 Ensembl ENCODE 22.1 +356.1 356069 8.8 HDGF PKNOX1 WRNIP1 SIN3A FEZF1 ZNF2 YY1 ZNF263 SP3 REST CSF3R MAP7D1 AGO4 THRAP3 STK40 RPL5P4 COL8A2 AGO3 SFPQ AGO1
GH01F036148 1.6 FANTOM5 ENCODE 3 +343.2 343242 4.9 HDGF PKNOX1 ARNT CREB3L1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 SFPQ RPL5P4 MAP7D1 EVA1B THRAP3 LOC105378647 STK40 COL8A2 AGO3 SH3D21
GH01F035962 0.4 FANTOM5 3.6 +155.1 155075 0.2 ZNF18 AGO3 AGO1 ADPRHL2 LOC100128093 TEKT2 AGO4 THRAP3 STK40 ENSG00000271914 LOC105378647
GH01F035965 0.3 FANTOM5 3.6 +158.0 158038 0.2 AGO4 AGO3 AGO1 STK40 ENSG00000271914 LOC105378647
GH01F035803 1.2 Ensembl ENCODE 0.8 -3.1 -3130 1.8 ATF1 MLX ARID4B DMAP1 ZNF48 YY1 ZNF121 NFYC MIER2 PPARG C1orf216 AGO4 CLSPN
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around AGO4 on UCSC Golden Path with GeneCards custom track

Promoters for AGO4 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001519164 970 2801 HDGF PKNOX1 ZNF2 ZNF766 GLIS2 GATA2 ELK1 KLF7 ZNF263 SP3

Genomic Location for AGO4 Gene

Chromosome:
1
Start:
35,807,630 bp from pter
End:
35,857,890 bp from pter
Size:
50,261 bases
Orientation:
Plus strand

Genomic View for AGO4 Gene

Genes around AGO4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AGO4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AGO4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AGO4 Gene

Proteins for AGO4 Gene

  • Protein details for AGO4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HCK5-AGO4_HUMAN
    Recommended name:
    Protein argonaute-4
    Protein Accession:
    Q9HCK5
    Secondary Accessions:
    • A7MD27

    Protein attributes for AGO4 Gene

    Size:
    861 amino acids
    Molecular mass:
    97097 Da
    Quaternary structure:
    • Interacts with EIF4B, IMP8, PRMT5, TNRC6A and TNRC6B (PubMed:19167051). Interacts with ZFP36 (PubMed:15766526).
    SequenceCaution:
    • Sequence=BAB13393.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for AGO4 Gene

Post-translational modifications for AGO4 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for AGO4 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for AGO4 (AGO4)

No data available for DME Specific Peptides for AGO4 Gene

Domains & Families for AGO4 Gene

Gene Families for AGO4 Gene

Suggested Antigen Peptide Sequences for AGO4 Gene

Graphical View of Domain Structure for InterPro Entry

Q9HCK5

UniProtKB/Swiss-Prot:

AGO4_HUMAN :
  • Contains 1 PAZ domain.
  • Belongs to the argonaute family. Ago subfamily.
Domain:
  • Contains 1 PAZ domain.
  • Contains 1 Piwi domain.
Family:
  • Belongs to the argonaute family. Ago subfamily.
genes like me logo Genes that share domains with AGO4: view

Function for AGO4 Gene

Molecular function for AGO4 Gene

UniProtKB/Swiss-Prot Function:
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).

Gene Ontology (GO) - Molecular Function for AGO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding IEA --
GO:0003723 RNA binding IEA --
GO:0003725 double-stranded RNA binding IDA 19966796
GO:0003727 single-stranded RNA binding IDA 19966796
GO:0005515 protein binding IPI 15766526
genes like me logo Genes that share ontologies with AGO4: view
genes like me logo Genes that share phenotypes with AGO4: view

Animal Models for AGO4 Gene

MGI Knock Outs for AGO4:

Animal Model Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for AGO4

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for AGO4 Gene

Localization for AGO4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AGO4 Gene

Cytoplasm, P-body.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for AGO4 gene
Compartment Confidence
nucleus 5
cytosol 5
plasma membrane 1
extracellular 1
mitochondrion 1
peroxisome 1
lysosome 1

Gene Ontology (GO) - Cellular Components for AGO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000932 cytoplasmic mRNA processing body IEA --
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA 15260970
GO:0005829 cytosol TAS --
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with AGO4: view

Pathways & Interactions for AGO4 Gene

genes like me logo Genes that share pathways with AGO4: view

Pathways by source for AGO4 Gene

Gene Ontology (GO) - Biological Process for AGO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006402 mRNA catabolic process IDA 18771919
GO:0006417 regulation of translation IEA --
GO:0007130 synaptonemal complex assembly IEA --
GO:0007140 male meiosis IEA --
GO:0007223 Wnt signaling pathway, calcium modulating pathway TAS --
genes like me logo Genes that share ontologies with AGO4: view

No data available for SIGNOR curated interactions for AGO4 Gene

Transcripts for AGO4 Gene

Unigene Clusters for AGO4 Gene

Argonaute RISC catalytic component 4:
Representative Sequences:

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for AGO4

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for AGO4 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b · 17c ^ 18 ^ 19 ^ 20
SP1: - -
SP2:
SP3: -
SP4: -
SP5:

Relevant External Links for AGO4 Gene

GeneLoc Exon Structure for
AGO4
ECgene alternative splicing isoforms for
AGO4

Expression for AGO4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for AGO4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for AGO4 Gene

This gene is overexpressed in Whole Blood (x4.8).

Protein differential expression in normal tissues from HIPED for AGO4 Gene

This gene is overexpressed in Tlymphocyte (20.1), Heart (12.8), Stomach (9.8), and Esophagus (7.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for AGO4 Gene



NURSA nuclear receptor signaling pathways regulating expression of AGO4 Gene:

AGO4

SOURCE GeneReport for Unigene cluster for AGO4 Gene:

Hs.744858
genes like me logo Genes that share expression patterns with AGO4: view

Primer Products

No data available for Protein tissue co-expression partners and mRNA Expression by UniProt/SwissProt for AGO4 Gene

Orthologs for AGO4 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for AGO4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia EIF2C4 34 35
  • 100 (n)
oppossum
(Monodelphis domestica)
Mammalia AGO4 35
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia AGO4 35
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia AGO4 34 35
  • 94.42 (n)
cow
(Bos Taurus)
Mammalia AGO4 34 35
  • 94.39 (n)
mouse
(Mus musculus)
Mammalia Ago4 34 16 35
  • 91.79 (n)
chicken
(Gallus gallus)
Aves AGO4 34
  • 85.14 (n)
lizard
(Anolis carolinensis)
Reptilia AGO4 35
  • 98 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100495218 34
  • 81.66 (n)
zebrafish
(Danio rerio)
Actinopterygii ago4 34 35
  • 80.26 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons ZLL 34
  • 52.16 (n)
rice
(Oryza sativa)
Liliopsida Os02g0831600 34
  • 53.35 (n)
corn
(Zea mays)
Liliopsida Zm.10165 34
Species where no ortholog for AGO4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for AGO4 Gene

ENSEMBL:
Gene Tree for AGO4 (if available)
TreeFam:
Gene Tree for AGO4 (if available)

Paralogs for AGO4 Gene

Paralogs for AGO4 Gene

(5) SIMAP similar genes for AGO4 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with AGO4: view

Variants for AGO4 Gene

Sequence variations from dbSNP and Humsavar for AGO4 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs10158259 -- 35,853,119(+) GGTGG(A/C)GGGCG intron-variant
rs10159354 -- 35,853,110(+) gccgg(A/G)tgtgg intron-variant
rs10615800 -- 35,841,837(+) ATATA(-/TA)CACCA intron-variant
rs10796923 -- 35,823,755(+) TTATT(A/T)TTTTT intron-variant, upstream-variant-2KB
rs111285434 -- 35,818,383(+) CTTTG(A/G)GAGGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for AGO4 Gene

Variant ID Type Subtype PubMed ID
nsv834680 CNV gain 17160897
nsv525430 CNV loss 19592680
nsv524683 CNV loss 19592680
nsv1130954 CNV deletion 24896259

Variation tolerance for AGO4 Gene

Residual Variation Intolerance Score: 4.21% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.68; 14.50% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for AGO4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
AGO4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for AGO4 Gene

Disorders for AGO4 Gene

Relevant External Links for AGO4

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
AGO4

No disorders were found for AGO4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for AGO4 Gene

Publications for AGO4 Gene

  1. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. (PMID: 19167051) Weinmann L. … Meister G. (Cell 2009) 3 4 64
  2. Importance of translation and nonnucleolytic ago proteins for on- target RNA interference. (PMID: 18771919) Wu L. … Belasco J.G. (Curr. Biol. 2008) 3 4 64
  3. Involvement of microRNA in AU-rich element-mediated mRNA instability. (PMID: 15766526) Jing Q. … Han J. (Cell 2005) 3 4 64
  4. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. (PMID: 15260970) Meister G. … Tuschl T. (Mol. Cell 2004) 3 4 64
  5. Identification of eight members of the Argonaute family in the human genome. (PMID: 12906857) Sasaki T. … Shimizu N. (Genomics 2003) 2 3 64

Products for AGO4 Gene

Sources for AGO4 Gene

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