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Aliases for AGO2 Gene

Aliases for AGO2 Gene

  • Argonaute RISC Catalytic Component 2 2 3 4
  • Eukaryotic Translation Initiation Factor 2C, 2 2 3
  • PAZ Piwi Domain Protein 3 4
  • Protein Slicer 3 4
  • Argonaute 2 2 3
  • Argonaute2 3 4
  • EIF-2C 2 3 4
  • EIF2C 2 3 4
  • EIF2C2 3 4
  • HAgo2 3 4
  • PPD 3 4
  • Eukaryotic Translation Initiation Factor 2C 2 4
  • Protein Argonaute-2 3
  • EC 3.1.26.n1 63
  • EC 3.1.26.n2 4
  • CTA-204B4.6 3
  • EIF2C2, 6
  • Q10 3

External Ids for AGO2 Gene

Previous HGNC Symbols for AGO2 Gene

  • EIF2C2

Previous GeneCards Identifiers for AGO2 Gene

  • GC08M141542

Summaries for AGO2 Gene

Entrez Gene Summary for AGO2 Gene

  • This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

GeneCards Summary for AGO2 Gene

AGO2 (Argonaute RISC Catalytic Component 2) is a Protein Coding gene. Among its related pathways are PI-3K cascade and PI-3K cascade. GO annotations related to this gene include mRNA binding and siRNA binding. An important paralog of this gene is PIWIL4.

UniProtKB/Swiss-Prot for AGO2 Gene

  • Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3 untranslated region (3-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Gene Wiki entry for AGO2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AGO2 Gene

Genomics for AGO2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for AGO2 Gene

Start:
140,531,165 bp from pter
End:
140,635,619 bp from pter
Size:
104,455 bases
Orientation:
Minus strand

Genomic View for AGO2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for AGO2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AGO2 Gene

No data available for Regulatory Elements for AGO2 Gene

Proteins for AGO2 Gene

  • Protein details for AGO2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UKV8-AGO2_HUMAN
    Recommended name:
    Protein argonaute-2
    Protein Accession:
    Q9UKV8
    Secondary Accessions:
    • Q8TCZ5
    • Q8WV58
    • Q96ID1

    Protein attributes for AGO2 Gene

    Size:
    859 amino acids
    Molecular mass:
    97208 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
    Quaternary structure:
    • Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced silencing complex (RISC). Together, DICER1, AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs rather than miRNAs appears to occur independently of DICER1. Interacts with AGO1. Also interacts with DDB1, DDX5, DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the interaction is modulated under stress-induced conditions, occurs under both cell proliferation and differentiation conditions and in an RNA- and phosphorylation-independent manner. Interacts with LIMD1, WTIP and AJUBA. Interacts with TRIM71. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with APOBEC3A, APOBEC3C, APOBEC3F and APOBEC3H.
    SequenceCaution:
    • Sequence=AAH07633.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAL76093.1; Type=Miscellaneous discrepancy; Note=cDNA contains a duplication of an internal sequence at the 5 end.; Evidence={ECO:0000305}; Sequence=BC125214; Type=Frameshift; Positions=450; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for AGO2 Gene

    Alternative splice isoforms for AGO2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for AGO2 Gene

Proteomics data for AGO2 Gene at MOPED

Post-translational modifications for AGO2 Gene

  • Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
  • Ubiquitination at Lys550
  • Modification sites at PhosphoSitePlus

Other Protein References for AGO2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for AGO2 Gene

Gene Families for AGO2 Gene

HGNC:
  • AGO :Argonaute/PIWI family

Graphical View of Domain Structure for InterPro Entry

Q9UKV8

UniProtKB/Swiss-Prot:

AGO2_HUMAN :
  • Q9UKV8
Domain:
  • The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However, while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH)
  • Contains 1 PAZ domain.
  • Contains 1 Piwi domain.
Family:
  • Belongs to the argonaute family. Ago subfamily.
genes like me logo Genes that share domains with AGO2: view

No data available for Suggested Antigen Peptide Sequences for AGO2 Gene

Function for AGO2 Gene

Molecular function for AGO2 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=1.1 nM for a synthetic 21-nucleotide single-stranded RNA {ECO:0000269 PubMed:15105377, ECO:0000269 PubMed:15800637};
UniProtKB/Swiss-Prot CatalyticActivity: Endonucleolytic cleavage to 5-phosphomonoester.
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by EDTA.
UniProtKB/Swiss-Prot Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3 untranslated region (3-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Enzyme Numbers (IUBMB) for AGO2 Gene

Gene Ontology (GO) - Molecular Function for AGO2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000340 RNA 7-methylguanosine cap binding IDA 17524464
GO:0000993 RNA polymerase II core binding IDA 25336585
GO:0001047 core promoter binding IMP 25336585
GO:0003676 nucleic acid binding --
GO:0003723 RNA binding --
genes like me logo Genes that share ontologies with AGO2: view
genes like me logo Genes that share phenotypes with AGO2: view

Animal Models for AGO2 Gene

MGI Knock Outs for AGO2:

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for AGO2

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for AGO2 Gene

Localization for AGO2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AGO2 Gene

Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.

Gene Ontology (GO) - Cellular Components for AGO2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000932 cytoplasmic mRNA processing body IDA 20616046
GO:0005634 nucleus IC 25336585
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IDA 15260970
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with AGO2: view

No data available for Subcellular locations from COMPARTMENTS for AGO2 Gene

Pathways for AGO2 Gene

genes like me logo Genes that share pathways with AGO2: view

Pathways by source for AGO2 Gene

3 Qiagen pathways for AGO2 Gene
1 Cell Signaling Technology pathway for AGO2 Gene

Gene Ontology (GO) - Biological Process for AGO2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated --
GO:0006379 mRNA cleavage --
GO:0006412 translation NAS 10534406
GO:0006413 translational initiation NAS 10534406
genes like me logo Genes that share ontologies with AGO2: view

Transcripts for AGO2 Gene

Unigene Clusters for AGO2 Gene

Argonaute RISC catalytic component 2:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for AGO2

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for AGO2
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for AGO2 Gene

No ASD Table

Relevant External Links for AGO2 Gene

GeneLoc Exon Structure for
AGO2
ECgene alternative splicing isoforms for
AGO2

Expression for AGO2 Gene

mRNA expression in normal human tissues for AGO2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for AGO2 Gene

This gene is overexpressed in Whole Blood (7.5) and Muscle - Skeletal (4.3).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for AGO2 Gene

SOURCE GeneReport for Unigene cluster for AGO2 Gene Hs.743313

genes like me logo Genes that share expressions with AGO2: view

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for AGO2 Gene

Orthologs for AGO2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for AGO2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia EIF2C2 35
  • 99.59 (n)
  • 100 (a)
EIF2C2 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia EIF2C2 35
  • 92.48 (n)
  • 99.88 (a)
EIF2C2 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia AGO2 35
  • 92.58 (n)
  • 99.88 (a)
AGO2 36
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ago2 35
  • 90.78 (n)
  • 99.76 (a)
Ago2 16
Ago2 36
  • 99 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia AGO2 36
  • 98 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia AGO2 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ago2 35
  • 90.14 (n)
  • 99.64 (a)
chicken
(Gallus gallus)
Aves AGO2 36
  • 98 (a)
OneToOne
LOC100858102 35
  • 81.71 (n)
  • 99.17 (a)
lizard
(Anolis carolinensis)
Reptilia AGO2 36
  • 97 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ago2 35
  • 79.96 (n)
  • 97.74 (a)
Str.15011 35
zebrafish
(Danio rerio)
Actinopterygii CU929240.1 36
  • 90 (a)
OneToOne
LOC570630 35
  • 79.94 (n)
  • 93.22 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP011717 35
  • 68.98 (n)
  • 76.44 (a)
fruit fly
(Drosophila melanogaster)
Insecta AGO1 35
  • 67.5 (n)
  • 75.96 (a)
worm
(Caenorhabditis elegans)
Secernentea alg-1 35
  • 62.53 (n)
  • 70.17 (a)
soybean
(Glycine max)
eudicotyledons Gma.5747 35
thale cress
(Arabidopsis thaliana)
eudicotyledons AGO5 35
  • 49.82 (n)
  • 43.08 (a)
Species with no ortholog for AGO2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for AGO2 Gene

ENSEMBL:
Gene Tree for AGO2 (if available)
TreeFam:
Gene Tree for AGO2 (if available)

Paralogs for AGO2 Gene

Paralogs for AGO2 Gene

Selected SIMAP similar genes for AGO2 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with AGO2: view

Variants for AGO2 Gene

Sequence variations from dbSNP and Humsavar for AGO2 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type MAF
rs868224 -- 140,608,684(-) CGCAG(C/T)GGAGG intron-variant
rs882958 -- 140,607,866(+) TGCGC(A/G)CCCAA intron-variant
rs883596 -- 140,625,549(+) GTCCT(C/G)TTTTG intron-variant
rs884934 -- 140,608,623(-) TGGCA(C/T)GCGGT intron-variant
rs884935 -- 140,608,550(-) CCGCC(C/T)CGGTC intron-variant

Relevant External Links for AGO2 Gene

HapMap Linkage Disequilibrium report
AGO2
Human Gene Mutation Database (HGMD)
AGO2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for AGO2 Gene

Disorders for AGO2 Gene

No disorders were found for AGO2 Gene.

No data available for UniProtKB/Swiss-Prot for AGO2 Gene

Publications for AGO2 Gene

  1. Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. (PMID: 14749716) Tahbaz N. … Hobman T.C. (EMBO Rep. 2004) 3 4 23
  2. A human, ATP-independent, RISC assembly machine fueled by pre- miRNA. (PMID: 16357216) Maniataki E. … Mourelatos Z. (Genes Dev. 2005) 3 4 23
  3. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. (PMID: 16756390) Chu C.-Y. … Rana T.M. (PLoS Biol. 2006) 3 4 23
  4. In vitro reconstitution of the human RISC-loading complex. (PMID: 18178619) MacRae I.J. … Doudna J.A. (Proc. Natl. Acad. Sci. U.S.A. 2008) 3 4 23
  5. Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. (PMID: 10534406) Koesters R. … Briner J. (Genomics 1999) 2 3 4

Products for AGO2 Gene

  • Addgene plasmids for AGO2

Sources for AGO2 Gene

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