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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

AGO2 Gene

protein-coding   GIFtS: 50
GCID: GC08M141542

Argonaute RISC Catalytic Component 2

(Previous names: eukaryotic translation initiation factor 2C, 2)
(Previous symbol: EIF2C2)
Microbiology & Infectious Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Argonaute RISC Catalytic Component 21 2 3     PAZ Piwi Domain Protein2 3
EIF2C21 2 3 5     Q102
Eukaryotic Translation Initiation Factor 2C, 21 2     argonaute22
Argonaute 21 2     Protein Argonaute-22
Protein Slicer2 3     Argonaute23
PPD2 3     EC 3.1.26.n23
EIF-2C 22 3     Eukaryotic Translation Initiation Factor 2C 23
EIF2C 22 3     EC 3.1.26.n18
hAgo22 3     

External Ids:    HGNC: 32631   Entrez Gene: 271612   Ensembl: ENSG000001239087   OMIM: 6062295   UniProtKB: Q9UKV83   

Export aliases for AGO2 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for AGO2 Gene:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded
protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role
in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have
been found for this gene. (provided by RefSeq, Sep 2009)

GeneCards Summary for AGO2 Gene: 
AGO2 (argonaute RISC catalytic component 2) is a protein-coding gene. Diseases associated with AGO2 include estrogen-receptor negative breast cancer, and skin cancer, and among its related super-pathways are Signaling by FGFR and Constitutive PI3K/AKT Signaling in Cancer. GO annotations related to this gene include mRNA binding and siRNA binding. An important paralog of this gene is PIWIL4.

UniProtKB/Swiss-Prot: AGO2_HUMAN, Q9UKV8
Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The
'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering
RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene
silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or
siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to
endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA
results in silencing through inhibition of translation, and this is independent of endonuclease activity. May
inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the
translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which
itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The
inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing
bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational
repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also
up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the
3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also
required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct
the transcriptional repression of complementary promoter regions

Gene Wiki entry for AGO2 (EIF2C2) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000008.10  NC_018919.2  NT_008046.16  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for AGO2
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for AGO2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AGO2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24   Ensembl cytogenetic band:  8q24.3   HGNC cytogenetic band: 8q24.3

AGO2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AGO2 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M141542:  view genomic region     (about GC identifiers)

Start:
141,541,264 bp from pter      End:
141,645,718 bp from pter
Size:
104,455 bases      Orientation:
minus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: AGO2_HUMAN, Q9UKV8 (See protein sequence)
Recommended Name: Protein argonaute-2  
Size: 859 amino acids; 97208 Da
Subunit: Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced
silencing complex (RISC). Together, DICER1, AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC),
or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process
precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA
constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term
RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs
rather than miRNAs appears to occur independently of DICER1. Interacts with AGO1. Also interacts with DDB1, DDX5,
DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10,
PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components
DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the
interaction is modulated under stress-induced conditions, occurs under both cell proliferation and
differentiation conditions and in a RNA- and phosphorylation-independent manner. Interacts with LIMD1, WTIP and
AJUBA. Interacts with TRIM71. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with APOBEC3A,
APOBEC3C, APOBEC3F and APOBEC3H
Subcellular location: Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their
recruitment to P-bodies. Translocation to the nucleus requires IMP8
Sequence caution: Sequence=AAH07633.1; Type=Erroneous initiation; Sequence=AAL76093.1; Type=Miscellaneous
discrepancy; Note=cDNA contains a duplication of an internal sequence at the 5' end; Sequence=BC125214;
Type=Frameshift; Positions=450;
6/11 PDB 3D structures from and Proteopedia for AGO2 (see all 11):
3LUC (3D)        3LUD (3D)        3LUG (3D)        3LUH (3D)        3LUJ (3D)        3LUK (3D)    
Secondary accessions: Q8TCZ5 Q8WV58 Q96ID1
Alternative splicing: 2 isoforms:  Q9UKV8-1   Q9UKV8-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for AGO2: NX_Q9UKV8

Explore proteomics data for AGO2 at MOPED 

Post-translational modifications:

  • UniProtKB: Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or
    endonuclease activity
  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q9UKV8

  • 4/10 DME Specific Peptides for AGO2 (Q9UKV8) (see all 10)
     CVSLQALHDALSGRLP  MTDVTGRVLQPPSILYGGR  NERVGKSGNIPAGTTVDTKITHP  QIFGDRKPVFDGRKNLYTAMPLPI 

    AGO2 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    AGO2 Protein Expression
    REFSEQ proteins (2 alternative transcripts): 
    NP_001158095.1  NP_036286.2  

    ENSEMBL proteins: 
     ENSP00000220592   ENSP00000430176   ENSP00000430164   ENSP00000431056  
    Reactome Protein details: Q9UKV8
    Human Recombinant Protein Products for AGO2: 
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    Novus Biologicals AGO2 Proteins
    Novus Biologicals AGO2 Lysate
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    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp. 

    Gene Ontology (GO): 5/9 cellular component terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000932cytoplasmic mRNA processing body IDA--
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005844polysome IDA17932509

    AGO2 for ontologies           About GeneDecksing



    AGO2 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of AGO2
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    Abcam antibodies for AGO2
    Browse Antibodies at Cloud-Clone Corp. 
    ThermoFisher Antibodies for AGO2
    Search LSBio for Antibodies for AGO2 

    Assay Products for AGO2: 
    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for AGO2
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp. 
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    AGO: Argonaute/PIWI family

    4 InterPro protein domains:
     IPR014811 DUF1785
     IPR003165 Piwi
     IPR003100 PAZ
     IPR012337 RNaseH-like_dom

    Graphical View of Domain Structure for InterPro Entry Q9UKV8

    ProtoNet protein and cluster: Q9UKV8

    2 Blocks protein domains:
    IPB003100 Argonaute and Dicer protein
    IPB003165 Stem cell self-renewal protein Piwi


    UniProtKB/Swiss-Prot: AGO2_HUMAN, Q9UKV8
    Domain: The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However, while RNase H
    utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of
    Asp-Asp-His (DDH)
    Similarity: Belongs to the argonaute family. Ago subfamily
    Similarity: Contains 1 PAZ domain
    Similarity: Contains 1 Piwi domain


    AGO2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: AGO2_HUMAN, Q9UKV8
    Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The
    'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering
    RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene
    silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or
    siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to
    endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA
    results in silencing through inhibition of translation, and this is independent of endonuclease activity. May
    inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the
    translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which
    itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The
    inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing
    bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational
    repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also
    up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the
    3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also
    required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct
    the transcriptional repression of complementary promoter regions
    Catalytic activity: Endonucleolytic cleavage to 5'-phosphomonoester
    Enzyme regulation: Inhibited by EDTA
    Biophysicochemical properties: Kinetic parameters: KM=1.1 nM for a synthetic 21-nucleotide single-stranded RNA;

         Enzyme Numbers (IUBMB): EC 3.1.26.n21 EC 3.1.26.n12

         Gene Ontology (GO): 5/10 molecular function terms (GO ID links to tree view) (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000340RNA 7-methylguanosine cap binding IDA17524464
    GO:0003676nucleic acid binding ----
    GO:0003723RNA binding ----
    GO:0003729mRNA binding IEA--
    GO:0003743translation initiation factor activity NAS10534406
         
    AGO2 for ontologies           About GeneDecksing


    Phenotypes:
         11 MGI mutant phenotypes (inferred from 6 alleles(MGI details for Ago2):
     cardiovascular system  cellular  embryogenesis  growth/size  hematopoietic system 
     immune system  integument  mortality/aging  nervous system  no phenotypic analysis 
     normal 

    AGO2 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Ago2tm1Ghan for AGO2

       inGenious Targeting Laboratory - Custom generated mouse model solutions for AGO2 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for AGO2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for AGO2 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for AGO2 

    miRNA
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    Inhib. RNA
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AGO2


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for AGO2 About   (see all 13)                                                                                              See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Signaling by FGFR
    Downstream signaling of activated FGFR0.92
    Downstream signal transduction0.90
    Signaling by FGFR0.92
    Signaling by PDGF0.85
    Signaling by FGFR in disease0.91
    Signaling by EGFR0.84
    DAP12 signaling0.91
    Signaling by EGFR in Cancer0.83
    2PIP3 activates AKT signaling
    GAB1 signalosome0.93
    PI3K events in ERBB2 signaling0.88
    PI3K/AKT activation0.93
    PI3K events in ERBB4 signaling0.88
    PIP3 activates AKT signaling0.88
    Signaling by SCF-KIT0.72
    PI-3K cascade0.88
    Signaling by ERBB40.67
    3Post-transcriptional Silencing By Small RNAs
    Post-transcriptional Silencing By Small RNAs0.58
    Small Interfering RNA (siRNA) Biogenesis0.40
    Pre-NOTCH Transcription and Translation0.58
    4Immune System
    Immune System0.56
    Innate Immune System0.50
    Adaptive Immune System0.56
    5Regulatory RNA pathways
    MicroRNA (miRNA) Biogenesis0.88
    Regulatory RNA pathways0.88

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for AGO2
        Translational Control

    5/38        Reactome Pathways for AGO2 (see all 38)
        Signaling by EGFR in Cancer
    Downstream signal transduction
    Signal Transduction
    Regulatory RNA pathways
    Signaling by PDGF



    AGO2 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for AGO2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/224 Interacting proteins for AGO2 (Q9UKV81, 2, 3 ENSP000002205924) via UniProtKB, MINT, STRING, and/or I2D (see all 224)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IPO8O153971, 2, 3, ENSP000002560794EBI-528269,EBI-358808 MINT-6489217 I2D: score=2 STRING: ENSP00000256079
    TARBP2Q156332, 3, ENSP000002669874MINT-6489217 MINT-1957947 I2D: score=2 STRING: ENSP00000266987
    GEMIN4P576782, 3, ENSP000003217064MINT-6489539 MINT-6489355 I2D: score=1 STRING: ENSP00000321706
    AGO3Q9H9G72, 3, ENSP000003622874MINT-6489217 I2D: score=2 STRING: ENSP00000362287
    DDX5P178442, 3, ENSP000002257924MINT-6167618 I2D: score=2 STRING: ENSP00000225792
    About this table

    Gene Ontology (GO): 5/21 biological process terms (GO ID links to tree view) (see all 21):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-dependent IEA--
    GO:0006355regulation of transcription, DNA-dependent IEA--
    GO:0006379mRNA cleavage ----
    GO:0006412translation NAS10534406
    GO:0006413translational initiation NAS10534406

    AGO2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for AGO2

    Search CenterWatch for drugs/clinical trials and news about AGO2

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for AGO2 gene (2 alternative transcripts): 
    NM_001164623.1  NM_012154.3  

    Unigene Cluster for AGO2:

    Argonaute RISC catalytic component 2
    Hs.743313  [show with all ESTs]
    Unigene Representative Sequence: BC054491
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000220592(uc003yvn.3 uc010meo.3 uc010men.3) ENST00000519980
    ENST00000523609 ENST00000520628 ENST00000520412 ENST00000519347 ENST00000521325
    ENST00000517293 ENST00000524328 ENST00000518019
    Congresses - knowledge worth sharing:  
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    Additional mRNA sequence: 

    AF121255.2 AF426412.1 AK096164.1 AK130012.1 AL050151.1 AY077717.1 BC007633.1 BC018727.1 
    BC033700.1 BC046220.1 BC054491.1 BC065217.1 BC125213.1 BC125214.1 BT007229.1 

    10 DOTS entries:

    DT.99950357  DT.95203659  DT.216895  DT.40270179  DT.40278352  DT.95269140  DT.95297668  DT.97817890 
    DT.97819402  DT.99984656 

    24/278 AceView cDNA sequences (see all 278):

    AW206791 AA287592 AW243902 BC054491 AI190650 BE675974 AI792645 N93082 
    AI636609 AW204784 AI359491 AW051276 CA389557 AL050151 AI584174 BQ581595 
    AA553353 BM464843 AA369545 AI868989 AA262137 AA281632 AI742020 CA391072 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    AGO2 expression in normal human tissues (normalized intensities)      AGO2 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    AGO2 Expression
    About this image


    AGO2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/29 selected tissues (see all 29) fully expand
     
     Epithelium
             vagina ; squamous epithelial cells   
     
     Testis (Reproductive System)    fully expand to see all 4 entries
             Leydig Cells Testis Interstitium
             seminal vesicle ; glandular cells   
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Uterus
             uterus, post-menopause ; glandular cells   
     
     Blood (Hematopoietic System)    fully expand to see all 2 entries
             Hematopoietic Stem Cells Hematopoietic Bone Marrow
             lung ; macrophages   

    See AGO2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for AGO2

    SOURCE GeneReport for Unigene cluster: Hs.743313
        SABiosciences Custom PCR Arrays for AGO2
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for AGO2 gene from 9/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ago25
    Eif2c21
    eukaryotic translation initiation factor 2C, 21, 5 90.61(n)1
    99.3(a)1
      15 (33.92 cM)5
    2395281  NM_153178.41  NP_694818.31 
     731016255 
    chicken
    (Gallus gallus)
    Aves LOC1008581021 protein argonaute-2-like 81.71(n)
    99.06(a)
      100858102  XM_003640870.1  XP_003640918.1 
    lizard
    (Anolis carolinensis)
    Reptilia EIF2C26
    Uncharacterized protein
    97(a)
    1 ↔ 1
    4(15623698-15657145)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.150112 Transcribed sequence with strong similarity to protein more 80.28(n)    BX743547.1 
    zebrafish
    (Danio rerio)
    Actinopterygii LOC5706301 protein argonaute-2-like 79.45(n)
    92.28(a)
      570630  XM_694134.2  XP_699226.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta AGO11 Argonaute-1 67.06(n)
    75(a)
      36544  NM_166020.2  NP_725341.1 
    worm
    (Caenorhabditis elegans)
    Secernentea alg-11 Protein ALG-1 61.87(n)
    69.36(a)
      181504  NM_077921.5  NP_510322.2 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AGO11 protein argonaute 51.87(n)
    46.77(a)
      841262  NM_001198240.1  NP_001185169.1 
    rice
    (Oryza sativa)
    Liliopsida Os02g06722001 hypothetical protein 52.34(n)
    46.4(a)
      4330281  NM_001054239.1  NP_001047704.1 


    ENSEMBL Gene Tree for AGO2 (if available)
    TreeFam Gene Tree for AGO2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for AGO2 gene
    PIWIL42  PIWIL22  AGO32  PIWIL12  AGO12  AGO42  PIWIL32  
    4 SIMAP similar genes for AGO2 using alignment to 3 protein entries:     AGO2_HUMAN (see all proteins):
    EIF2C2    AGO1    AGO3    AGO4

    AGO2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2427 SNPs in AGO2 are shown (see all 2427)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1872344701,2
    --141540813(+) TCCTTC/TGCTGT 2 -- ds50010--------
    rs37357971,2
    C,F--141540826(+) CCGACG/AGCCTC 2 -- ds500110Minor allele frequency- A:0.09EA NA WA 1838
    rs1892072611,2
    --141540833(+) CCTCAC/GGCCCA 2 -- ds50010--------
    rs1505381081,2
    --141540940(+) CATCAA/GTGTTT 2 -- ds50010--------
    rs1383617761,2
    --141541039(+) AGCGGC/TGCGGA 2 -- ds50010--------
    rs37357981,2
    C,F--141541042(+) GGCGCG/AGAGGG 2 -- ds50019Minor allele frequency- A:0.07EA NA WA 1978
    rs29774611,2
    C,F,A,H--141541288(+) ATATTC/TTTCTC 2 -- ut31 ese3 trp322Minor allele frequency- T:0.28NA NS EA WA CSA 2371
    rs1492504661,2
    --141541864(+) TTTAGA/GAGATT 2 -- ut310--------
    rs798743131,2
    C--141541868(+) GGAGAC/TTTTTA 2 -- ut310--------
    rs776931581,2
    C--141541870(+) AGATTC/TTTAGG 2 -- ut310--------

    HapMap Linkage Disequilibrium report for AGO2 (141541264 - 141645718 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for AGO2: --
    SABiosciences Cancer Mutation PCR Assays
    SeqTarget long-range PCR primers for resequencing AGO2
    DNA2.0 Custom Variant and Variant Library Synthesis for AGO2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 606229    OMIM disorders: --

    18 diseases for AGO2:    About MalaCards
    estrogen-receptor negative breast cancer    skin cancer    esophageal cancer    huntington's disease
    myeloma    hepatitis c    renal cell carcinoma    esophagitis
    neuroblastoma    hypoxia    squamous cell carcinoma    colorectal cancer
    lung cancer    endotheliitis    hepatitis    prostatitis
    breast cancer    neuronitis


    AGO2 for disorders           About GeneDecksing


    Export disorders for AGO2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for AGO2 gene, integrated from 9 sources (see all 177):
    (articles sorted by number of sources associating them with AGO2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. (PubMed id 10534406)1, 2, 3 Koesters R....Briner J. (1999)
    2. A human, ATP-independent, RISC assembly machine fueled by pre- miRNA. (PubMed id 16357216)1, 2, 9 Maniataki E. and Mourelatos Z. (2005)
    3. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. (PubMed id 16756390)1, 2, 9 Chu C.-Y. and Rana T.M. (2006)
    4. In vitro reconstitution of the human RISC-loading complex. (PubMed id 18178619)1, 2, 9 MacRae I.J....Doudna J.A. (2008)
    5. Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. (PubMed id 14749716)1, 2, 9 Tahbaz N....Hobman T.C. (2004)
    6. APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10. (PubMed id 22791714)1, 2 Liu C....Zhang H. (2012)
    7. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. (PubMed id 19167051)1, 2 Weinmann L.... Meister G. (2009)
    8. RNA-binding motif protein 4 translocates to cytoplasm ic granules and suppresses translation via argonaute2 during muscle cell differ entiation. (PubMed id 19801630)1, 2 Lin J.C. and Tarn W.Y. (2009)
    9. Prolyl 4-hydroxylation regulates Argonaute 2 stability. (PubMed id 18690212)1, 2 Qi H.H....Shi Y. (2008)
    10. Importance of translation and nonnucleolytic ago proteins for on- target RNA interference. (PubMed id 18771919)1, 2 Wu L.... Belasco J.G. (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 27161 HGNC: 3263 AceView: EIF2C2 Ensembl:ENSG00000123908 euGenes: HUgn27161
    ECgene: AGO2 H-InvDB: AGO2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for AGO2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for AGO2 gene:
    Search GeneIP for patents involving AGO2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    About This Section

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