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AGO2 Gene

protein-coding   GIFtS: 48
GCID: GC08M141542

Argonaute RISC Catalytic Component 2

(Previous names: eukaryotic translation initiation factor 2C, 2)
(Previous symbol: EIF2C2)
  Search for AGO2
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Argonaute RISC Catalytic Component 21 2 3     PAZ Piwi Domain Protein2 3
EIF2C21 2 3 5     Q102
Eukaryotic Translation Initiation Factor 2C, 21 2     argonaute22
Argonaute 21 2     Protein Argonaute-22
Protein Slicer2 3     Argonaute23
PPD2 3     EC 3.1.26.n23
EIF-2C 22 3     Eukaryotic Translation Initiation Factor 2C 23
EIF2C 22 3     EC 3.1.26.n18
hAgo22 3     

External Ids:    HGNC: 32631   Entrez Gene: 271612   Ensembl: ENSG000001239087   OMIM: 6062295   UniProtKB: Q9UKV83   

Export aliases for AGO2 gene to outside databases


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for AGO2 Gene:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded
protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role
in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have
been found for this gene. (provided by RefSeq, Sep 2009)

GeneCards Summary for AGO2 Gene:
AGO2 (argonaute RISC catalytic component 2) is a protein-coding gene. GO annotations related to this gene include mRNA binding and siRNA binding. An important paralog of this gene is PIWIL4.

UniProtKB/Swiss-Prot: AGO2_HUMAN, Q9UKV8
Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The
'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering
RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene
silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or
siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to
endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA
results in silencing through inhibition of translation, and this is independent of endonuclease activity. May
inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the
translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which
itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The
inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing
bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational
repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also
up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the
3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also
required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct
the transcriptional repression of complementary promoter regions

Gene Wiki entry for AGO2 (EIF2C2) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Search for regulatory transcription factor binding sites for AGO2
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for AGO2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat AGO2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24   Ensembl cytogenetic band:  8q24.3   HGNC cytogenetic band: 8q24.3

AGO2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AGO2 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M141542:  view genomic region     (about GC identifiers)

Start:
141,541,264 bp from pter      End:
141,645,718 bp from pter
Size:
104,455 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: AGO2_HUMAN, Q9UKV8 (See protein sequence)
Recommended Name: Protein argonaute-2  
Size: 859 amino acids; 97208 Da
Subunit: Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced
silencing complex (RISC). Together, DICER1, AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC),
or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process
precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA
constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term
RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs
rather than miRNAs appears to occur independently of DICER1. Interacts with AGO1. Also interacts with DDB1, DDX5,
DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10,
PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components
DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the
interaction is modulated under stress-induced conditions, occurs under both cell proliferation and
differentiation conditions and in an RNA- and phosphorylation-independent manner. Interacts with LIMD1, WTIP and
AJUBA. Interacts with TRIM71. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with APOBEC3A,
APOBEC3C, APOBEC3F and APOBEC3H
Sequence caution: Sequence=AAH07633.1; Type=Erroneous initiation; Sequence=AAL76093.1; Type=Miscellaneous
discrepancy; Note=cDNA contains a duplication of an internal sequence at the 5' end; Sequence=BC125214;
Type=Frameshift; Positions=450;
Selected PDB 3D structures from and Proteopedia for AGO2 (see all 11):
3LUC (3D)        3LUD (3D)        3LUG (3D)        3LUH (3D)        3LUJ (3D)        3LUK (3D)    
Secondary accessions: Q8TCZ5 Q8WV58 Q96ID1
Alternative splicing: 2 isoforms:  Q9UKV8-1   Q9UKV8-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for AGO2: NX_Q9UKV8

Explore proteomics data for AGO2 at MOPED

Post-translational modifications: 

  • Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or
    endonuclease activity1
  • Ubiquitination2 at Lys550
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for AGO2 (Q9UKV8) (see all 10)
     CVSLQALHDALSGRLP  MTDVTGRVLQPPSILYGGR  NERVGKSGNIPAGTTVDTKITHP  QIFGDRKPVFDGRKNLYTAMPLPI 


    See AGO2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001158095.1  NP_036286.2  

    ENSEMBL proteins: 
     ENSP00000220592   ENSP00000430176   ENSP00000430164   ENSP00000431056  
    Reactome Protein details: Q9UKV8

    AGO2 Human Recombinant Protein Products:

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    Novus Biologicals AGO2 Proteins
    Novus Biologicals AGO2 Lysate
    Sino Biological Recombinant Protein for AGO2
    Sino Biological Cell Lysate for AGO2
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    AGO2 Assay Products:

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    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for AGO2 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    AGO: Argonaute/PIWI family

    5 InterPro protein domains:
     IPR014811 DUF1785
     IPR003165 Piwi
     IPR003100 PAZ_dom
     IPR028602 AGO2
     IPR012337 RNaseH-like_dom

    Graphical View of Domain Structure for InterPro Entry Q9UKV8

    ProtoNet protein and cluster: Q9UKV8

    2 Blocks protein domains:
    IPB003100 Argonaute and Dicer protein
    IPB003165 Stem cell self-renewal protein Piwi


    UniProtKB/Swiss-Prot: AGO2_HUMAN, Q9UKV8
    Domain: The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However, while RNase H
    utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of
    Asp-Asp-His (DDH)
    Similarity: Belongs to the argonaute family. Ago subfamily
    Similarity: Contains 1 PAZ domain
    Similarity: Contains 1 Piwi domain


    Find genes that share domains with AGO2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: AGO2_HUMAN, Q9UKV8
    Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The
    'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering
    RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene
    silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or
    siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to
    endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA
    results in silencing through inhibition of translation, and this is independent of endonuclease activity. May
    inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the
    translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which
    itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The
    inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing
    bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational
    repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also
    up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the
    3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also
    required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct
    the transcriptional repression of complementary promoter regions
    Catalytic activity: Endonucleolytic cleavage to 5'-phosphomonoester
    Enzyme regulation: Inhibited by EDTA
    Biophysicochemical properties: Kinetic parameters: KM=1.1 nM for a synthetic 21-nucleotide single-stranded RNA;

         Enzyme Numbers (IUBMB): EC 3.1.26.n21 EC 3.1.26.n12

         Gene Ontology (GO): Selected molecular function terms (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000340RNA 7-methylguanosine cap binding IDA17524464
    GO:0003676nucleic acid binding ----
    GO:0003723RNA binding ----
    GO:0003729mRNA binding IEA--
    GO:0003743translation initiation factor activity NAS10534406
         
    Find genes that share ontologies with AGO2           About GenesLikeMe


    Phenotypes:
         12 MGI mutant phenotypes (inferred from 6 alleles(MGI details for Ago2):
     cardiovascular system  cellular  embryogenesis  growth/size/body  hematopoietic system 
     homeostasis/metabolism  immune system  integument  mortality/aging  nervous system 
     no phenotypic analysis  normal 

    Find genes that share phenotypes with AGO2           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Ago2tm1Ghan for AGO2

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for AGO2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for AGO2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for AGO2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for AGO2

    miRNA
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    miRTarBase miRNAs that target AGO2:
    hsa-mir-99a-5p (MIRT027394), hsa-mir-16-5p (MIRT031917), hsa-mir-192-5p (MIRT026680), hsa-mir-21-5p (MIRT031016), hsa-mir-30d-5p (MIRT047789), hsa-let-7a-5p (MIRT052575), hsa-mir-186-5p (MIRT045258), hsa-mir-671-5p (MIRT039278), hsa-mir-652-3p (MIRT039512), hsa-let-7e-5p (MIRT051431), hsa-mir-30a-5p (MIRT049935), hsa-mir-215-5p (MIRT024741), hsa-mir-376a-3p (MIRT007020), hsa-let-7b-5p (MIRT052006)

    Block miRNA regulation of human, mouse, rat AGO2 using miScript Target Protectors
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    SwitchGear 3'UTR luciferase reporter plasmidAGO2 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat AGO2

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    Addgene plasmids for AGO2 

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AGO2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    AGO2_HUMAN, Q9UKV8: Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their
    recruitment to P-bodies. Translocation to the nucleus requires IMP8

    Gene Ontology (GO): Selected cellular component terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000932cytoplasmic mRNA processing body IDA--
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005844polysome IDA17932509

    Find genes that share ontologies with AGO2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for AGO2 About   (see all 12)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Signaling by FGFR
    Signaling by FGFR0.91
    DAP12 interactions0.89
    Signaling by FGFR in disease0.91
    Signaling by PDGF0.87
    Downstream signaling of activated FGFR0.91
    Signaling by EGFR0.83
    Downstream signal transduction0.90
    Signaling by EGFR in Cancer0.82
    2PI-3K cascade
    PI3K/AKT activation0.93
    PI3K events in ERBB4 signaling0.88
    GAB1 signalosome0.93
    Role of LAT2/NTAL/LAB on calcium mobilization0.80
    PIP3 activates AKT signaling0.88
    Signaling by SCF-KIT0.72
    PI-3K cascade0.88
    Signaling by ERBB40.66
    3Regulatory RNA pathways
    MicroRNA (miRNA) Biogenesis0.88
    Post-transcriptional Silencing By Small RNAs0.00
    Regulatory RNA pathways0.88
    Small Interfering RNA (siRNA) Biogenesis0.00
    4beta-catenin independent WNT signaling
    beta-catenin independent WNT signaling0.79
    Ca2+ pathway0.43
    Signaling by Wnt0.79
    5Downstream Signaling Events Of B Cell Receptor (BCR)
    Downstream Signaling Events Of B Cell Receptor (BCR)0.79
    Signaling by the B Cell Receptor (BCR)0.79


    Find genes that share SuperPaths with AGO2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for AGO2
        RNAi Pathway
    GSK3 Signaling
    eIF2 Pathway

    1 Cell Signaling Technology (CST) Pathway for AGO2
        Translational Control


    Selected Reactome Pathways for AGO2 (see all 6)
        MicroRNA (miRNA) biogenesis
    Pre-NOTCH Transcription and Translation
    Ca2+ pathway
    Post-transcriptional silencing by small RNAs
    PIP3 activates AKT signaling


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for AGO2
    Interactions:

        Search GeneGlobe Interaction Network for AGO2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for AGO2 (Q9UKV81, 2, 3 ENSP000002205924) via UniProtKB, MINT, STRING, and/or I2D (see all 274)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TARBP2Q156331, 2, 3, ENSP000002669874EBI-528269,EBI-978581 MINT-6489217 MINT-1957947 I2D: score=2 STRING: ENSP00000266987
    IPO8O153971, 2, 3, ENSP000002560794EBI-528269,EBI-358808 MINT-6489217 I2D: score=2 STRING: ENSP00000256079
    TNRC6BQ9UPQ91, 2, 3, ENSP000003383714EBI-528269,EBI-947158 MINT-6489217 I2D: score=1 STRING: ENSP00000338371
    ZMAT3Q9HA381, 2EBI-528269,EBI-2548480 MINT-8410974 MINT-8410991 MINT-8410958 MINT-8410942 MINT-8411008
    GEMIN4P576782, 3, ENSP000003217064MINT-6489539 MINT-6489355 I2D: score=1 STRING: ENSP00000321706
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 22):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated IEA--
    GO:0006379mRNA cleavage ----
    GO:0006412translation NAS10534406
    GO:0006413translational initiation NAS10534406

    Find genes that share ontologies with AGO2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

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    Browse Small Molecules at EMD Millipore
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      Browse compounds at ApexBio 

    Browse Tocris compounds for AGO2



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for AGO2 gene (2 alternative transcripts): 
    NM_001164623.1  NM_012154.3  

    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000220592(uc003yvn.3 uc010meo.3 uc010men.3) ENST00000519980
    ENST00000523609 ENST00000520628 ENST00000520412 ENST00000519347 ENST00000521325
    ENST00000517293 ENST00000524328 ENST00000518019
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      QuantiFast Probe-based Assays in human, mouse, rat AGO2

    Selected AceView cDNA sequences (see all 278):

    BX116894 AL050151 BC054491 N93082 AW206791 AI792645 BE675974 CA389557 
    AA287592 D62032 AI636609 AA694381 AW204784 AA768827 AI584174 R91151 
    AI379307 AA722211 AI264284 AI359491 AI383270 AI263575 AI190650 AI376844 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    AGO2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    AGO2 Expression
    About this image


    AGO2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Pancreas (Endocrine System)
             Islets of Langerhans
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Liver (Hepatobiliary System)
             Hepatocytes Liver Lobule
    AGO2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    AGO2 Protein Expression
        Custom PCR Arrays for AGO2
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for AGO2 gene from Selected species (see all 18)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ago21 , 5 eukaryotic translation initiation factor 2C, 25
    argonaute RISC catalytic subunit 21
    90.78(n)1
    99.76(a)1
      15 (33.92 cM)5
    2395281  NM_153178.41  NP_694818.31 
     730958445 
    chicken
    (Gallus gallus)
    Aves LOC1008581021 protein argonaute-2-like 81.71(n)
    99.17(a)
      100858102  XM_004940065.1  XP_004940122.1 
    lizard
    (Anolis carolinensis)
    Reptilia AGO26
    argonaute RISC catalytic component 2
    97(a)
    1 ↔ 1
    4(15623698-15657145)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.150112 Transcribed sequence with strong similarity to protein more 80.28(n)    BX743547.1 
    zebrafish
    (Danio rerio)
    Actinopterygii LOC5706301 protein argonaute-2-like 79.94(n)
    93.22(a)
      570630  XM_694134.3  XP_699226.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta AGO11 Argonaute-1 67.5(n)
    75.96(a)
      36544  NM_001259385.1  NP_001246314.1 
    worm
    (Caenorhabditis elegans)
    Secernentea alg-11 alg-1 62.53(n)
    70.17(a)
      181504  NM_001270309.1  NP_001257238.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AGO51 AGO5 49.82(n)
    43.08(a)
      817334  NM_179779.2  NP_850110.1 
    barley
    (Hordeum vulgare)
    Liliopsida BQ461393.12   -- 73.82(n)    BQ461393.1 


    ENSEMBL Gene Tree for AGO2 (if available)
    TreeFam Gene Tree for AGO2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for AGO2 gene
    PIWIL42  PIWIL22  AGO32  PIWIL12  AGO12  AGO42  PIWIL32  
    5 SIMAP similar genes for AGO2 using alignment to 3 protein entries:     AGO2_HUMAN (see all proteins):
    EIF2C2    AGO1    AGO3    AGO4    EIF2C3

    Find genes that share paralogs with AGO2           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for AGO2 (see all 2427)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1872344701,2
    --141540813(+) TCCTTC/TGCTGT 2 -- ds50010--------
    rs37357971,2
    C,F--141540826(+) CCGACG/AGCCTC 2 -- ds500110Minor allele frequency- A:0.09EA NA WA 1838
    rs1892072611,2
    --141540833(+) CCTCAC/GGCCCA 2 -- ds50010--------
    rs1505381081,2
    --141540940(+) CATCAA/GTGTTT 2 -- ds50010--------
    rs1383617761,2
    --141541039(+) AGCGGC/TGCGGA 2 -- ds50010--------
    rs37357981,2
    C,F--141541042(+) GGCGCG/AGAGGG 2 -- ds50019Minor allele frequency- A:0.07EA NA WA 1978
    rs29774611,2
    C,F,A,H--141541288(+) ATATTC/TTTCTC 2 -- ut31 ese3 trp322Minor allele frequency- T:0.28NA NS EA WA CSA 2371
    rs1492504661,2
    --141541864(+) TTTAGA/GAGATT 2 -- ut310--------
    rs798743131,2
    C--141541868(+) GGAGAC/TTTTTA 2 -- ut310--------
    rs776931581,2
    C--141541870(+) AGATTC/TTTAGG 2 -- ut310--------

    HapMap Linkage Disequilibrium report for AGO2 (141541264 - 141645718 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for AGO2: --
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing AGO2
    DNA2.0 Custom Variant and Variant Library Synthesis for AGO2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 606229    OMIM disorders: --


    Find genes that share disorders with AGO2           About GenesLikeMe


    Export disorders for AGO2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for AGO2 gene, integrated from 10 sources (see all 185):
    (articles sorted by number of sources associating them with AGO2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. (PubMed id 10534406)1, 2, 3 Koesters R....Briner J. (Genomics 1999)
    2. A human, ATP-independent, RISC assembly machine fueled by pre- miRNA. (PubMed id 16357216)1, 2, 9 Maniataki E. and Mourelatos Z. (Genes Dev. 2005)
    3. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. (PubMed id 16756390)1, 2, 9 Chu C.-Y. and Rana T.M. (PLoS Biol. 2006)
    4. In vitro reconstitution of the human RISC-loading complex. (PubMed id 18178619)1, 2, 9 MacRae I.J.... Doudna J.A. (Proc. Natl. Acad. Sci. U.S.A. 2008)
    5. Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. (PubMed id 14749716)1, 2, 9 Tahbaz N....Hobman T.C. (EMBO Rep. 2004)
    6. APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10. (PubMed id 22791714)1, 2 Liu C....Zhang H. (J. Biol. Chem. 2012)
    7. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. (PubMed id 19167051)1, 2 Weinmann L.... Meister G. (Cell 2009)
    8. RNA-binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 during muscle cell differentiation. (PubMed id 19801630)1, 2 Lin J.C. and Tarn W.Y. (J. Biol. Chem. 2009)
    9. Prolyl 4-hydroxylation regulates Argonaute 2 stability. (PubMed id 18690212)1, 2 Qi H.H.... Shi Y. (Nature 2008)
    10. Importance of translation and nonnucleolytic ago proteins for on- target RNA interference. (PubMed id 18771919)1, 2 Wu L.... Belasco J.G. (Curr. Biol. 2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 27161 HGNC: 3263 AceView: EIF2C2 Ensembl:ENSG00000123908 euGenes: HUgn27161
    ECgene: AGO2 H-InvDB: AGO2

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for AGO2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for AGO2 gene:
    Search GeneIP for patents involving AGO2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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