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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

AGFG2 Gene

protein-coding   GIFtS: 49
GCID: GC07P100136

ArfGAP with FG repeats 2

(Previous name: HIV-1 Rev binding protein-like )
(Previous symbol: HRBL)
 Explore 2 diseases affiliated with
AGFG2 via our new
 Human Malady Compendium 
Biological research products
for AGFG2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
ArfGAP With FG Repeats 21 2     Arf-GAP Domain And FG Repeat-Containing Protein 22
HRBL1 2 3 5     Arf-GAP Domain And FG Repeats-Containing Protein 22
RABR1 2 3 5     Nucleoporin1
Rev/Rex Activation Domain-Binding Protein Related2 3     Rev/Rex Activation Domain Binding Protein-Related2
HIV-1 Rev-Binding Protein-Like Protein2 3     RAB-R3
HIV-1 Rev Binding Protein-Like1     

External Ids:    HGNC: 51771   Entrez Gene: 32682   Ensembl: ENSG000001063517   OMIM: 6040195   UniProtKB: O950813   

Export aliases for AGFG2 gene to outside databases

Previous GC identifers: GC07P099976 GC07P094769


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for AGFG2:
This gene is a member of the HIV-1 Rev binding protein (HRB) family and encodes a protein with one Arf-GAP zinc finger
domain, several phe-gly (FG) motifs, and four asn-pro-phe (NPF) motifs. This protein interacts with Eps15 homology
(EH) domains and plays a role in the Rev export pathway, which mediates the nucleocytoplasmic transfer of proteins and
RNAs. Alternatively spliced transcript variants have been described, but their biological validity has not been
determined. (provided by RefSeq, Feb 2013)

Gene Wiki entry for AGFG2 (HRBL)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000007.13  NC_018918.1  NT_007933.15  NT_079595.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the AGFG2 gene promoter:
         c-Fos   AML1a   Tal-1   AP-1   ATF-2   E47   Egr-4   Gfi-1   c-Myb   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 5): AGFG2 promoter sequence
   Search SABiosciences Chromatin IP Primers for AGFG2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AGFG2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q22.1   Ensembl cytogenetic band:  7q22.1   HGNC cytogenetic band: 7q22.1

AGFG2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AGFG2 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07P100136:  view genomic region     (about GC identifiers)

Start:
100,136,834 bp from pter      End:
100,165,843 bp from pter
Size:
29,010 bases      Orientation:
plus strand

1 alternative location:
Chr7+,CRA_TCAG 99,496,460-99,525,478     

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: AGFG2_HUMAN, O95081 (See protein sequence)
Recommended Name: Arf-GAP domain and FG repeat-containing protein 2  
Size: 481 amino acids; 48963 Da
Subunit: Interacts with EPS15R (By similarity)
Sequence caution: Sequence=AAC78803.1; Type=Erroneous gene model prediction;
Secondary accessions: O75429 Q96AB9 Q96GL4
Alternative splicing: 2 isoforms:  O95081-1   O95081-2   

Explore the universe of human proteins at neXtProt for AGFG2: NX_O95081

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O95081

  • AGFG2 Protein expression data from MOPED and PaxDb:    About this image 
    AGFG2 Protein Expression
    REFSEQ proteins: NP_006067.3  
    ENSEMBL proteins: 
     ENSP00000394453   ENSP00000300176   ENSP00000388594   ENSP00000262935  

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    Uscn Proteins for AGFG2

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575cellular_component ND--

    AGFG2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    AGFG2 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR001164 ArfGAP

    Graphical View of Domain Structure for InterPro Entry O95081

    ProtoNet protein and cluster: O95081

    1 Blocks protein family: IPB001164 HIV Rev interacting protein signature

    UniProtKB/Swiss-Prot: AGFG2_HUMAN, O95081
    Domain: Contains FG repeats and 4 N-P-F repeats
    Similarity: Contains 1 Arf-GAP domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

         Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--
    GO:0008060ARF GTPase activator activity IEA--
    GO:0008270zinc ion binding IEA--
         
    AGFG2 for ontologies           About GeneDecksing


    Phenotypes:
         3 GenomeRNAi human phenotypes for AGFG2:
     Cell cycle / mitosis defect  Decreased viability of wild-ty  Increased gamma-H2AX phosphory 

    Animal Models:
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for AGFG2 

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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for AGFG2

    STRING Interaction Network Preview (showing 3 interactants - click image to see more details)

    3 Interacting proteins for AGFG2 (O950813 ENSP000003001764) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EPS15L1Q9UBC23, ENSP000002480704I2D: score=1 STRING: ENSP00000248070
    EPS15P425663, ENSP000003607984I2D: score=3 STRING: ENSP00000360798
    ITSN1ENSP000003707194STRING: ENSP00000370719
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008150biological_process ND--
    GO:0032312regulation of ARF GTPase activity IEA--

    AGFG2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for AGFG2
    Search CenterWatch for drugs/clinical trials and news about AGFG2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for AGFG2 gene: 
    NM_006076.4  

    Unigene Cluster for AGFG2:

    ArfGAP with FG repeats 2
    Hs.521083  [show with all ESTs]
    Unigene Representative Sequence: NM_006076
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000430857 ENST00000477022(uc003uvg.1) ENST00000300176(uc003uvf.3)
    ENST00000474713 ENST00000429987 ENST00000262935

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    hsa-miR-520e hsa-miR-520f hsa-miR-1321 hsa-miR-15a hsa-miR-106a hsa-miR-200a hsa-miR-128 hsa-miR-374a
    SwitchGear 3'UTR luciferase reporter plasmidAGFG2 3' UTR sequence
    Inhib. RNA
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    Additional cDNA sequence: 

    AF015042.1 AK057758.1 AK090744.1 AK314954.1 BC009393.2 BC017329.2 BC047227.1 

    7 DOTS entries:

    DT.97774498  DT.100713995  DT.100695550  DT.424889  DT.100747284  DT.97839900  DT.95297758 

    24/99 AceView cDNA sequences (see all 99):

    BX105833 BM547827 T40281 CR626460 AX746598 BC017329 BU608908 CK429601 
    AK090744 BQ943086 CR597239 CR611659 BF843184 AK057758 NM_006076 BC009393 
    BX482301 AA551469 BM716210 BM545728 CA311591 BU539855 BQ883478 BU732186 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for AGFG2    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13
    SP1:                                      -     -                                       
    SP2:                    -     -                                                         
    SP3:                                      -                                             


    ECgene alternative splicing isoforms for AGFG2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    AGFG2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --
    AGFG2 Expression
    About this image
    See AGFG2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for AGFG2

    SOURCE GeneReport for Unigene cluster: Hs.521083
        SABiosciences Custom PCR Arrays for AGFG2

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for AGFG2 gene from 5/19 species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia AGFG26
    --
    51(a)
    1 ↔ 1
    GL344799.1(2324-8948)
    zebrafish
    (Danio rerio)
    Actinopterygii agfg1b6
    agfg1a6
    ArfGAP with FG repeats 1a
    35(a)
    35(a)
    many ↔ many
    many ↔ many
    2(16280904-16299874)
    15(34452669-34520655)
    fruit fly
    (Drosophila melanogaster)
    Insecta drongo6
    drongo
    16(a)
    1 → many
    2L(833584-851096)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons NIG1 NSP (nuclear shuttle protein)-interacting GTPase 38.19(n)
    30.07(a)
      826966  NM_117409.4  NP_193071.2 
    rice
    (Oryza sativa)
    Liliopsida Os02g02089001 hypothetical protein 51.52(n)
    39.39(a)
      4328692  NM_001052797.1  NP_001046262.1 


    ENSEMBL Gene Tree for AGFG2 (if available)
    TreeFam Gene Tree for AGFG2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for AGFG2 gene
    AGFG12  
    2 SIMAP similar genes for AGFG2 using alignment to 2 protein entries:     AGFG2_HUMAN (see all proteins):
    HRBL    AGFG1

    AGFG2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/446 NCBI SNPs in AGFG2 are shown (see all 446    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 7 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1857031401,2
    Cother100165881(+) GGGCCC/TGGAGT 1 -- ds50010--------
    rs78054981,2
    C--100134920(+) aatcgA/Cttgaa 1 -- us2k10--------
    rs1878695861,2
    --100134952(+) TGGGCA/GGATCA 1 -- us2k10--------
    rs1504040341,2
    --100135061(+) GTACTC/TCCAGC 1 -- us2k10--------
    rs1912291491,2
    --100135166(+) AAAGCC/GAGACC 1 -- us2k10--------
    rs1997209461,2
    --100135178(+) GTCTC-/AAAAAA 1 -- us2k10--------
    rs1147950851,2
    F--100135180(+) TCTCAA/GAAAAA 1 -- us2k11Minor allele frequency- G:0.03WA 118
    rs1157049461,2
    --100135205(+) AAAATG/AGTGAC 1 -- us2k11Minor allele frequency- A:0.01WA 118
    rs1837726821,2
    --100135289(+) CCAAGG/TCGGGA 1 -- us2k10--------
    rs1870133501,2
    --100135293(+) GGCGGA/GAGGCT 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for AGFG2 (100136834 - 100165843 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for AGFG2
         1 CNV: 4550

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    AGFG2 for disorders           About GeneDecksing

    OMIM gene information: 604019    OMIM disorders: --

    2 diseases for AGFG2:    About MalaCards
    xerophthalmia    bronchiectasis

    2 diseases from the University of Copenhagen DISEASES database for AGFG2:
    Bronchiectasis     Xerophthalmia

    Export disorders for AGFG2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for AGFG2 gene, integrated from 9 sources (see all 15):
    (articles sorted by number of sources associating them with AGFG2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes. (PubMed id 9799793)1, 2, 3 Gloeckner G.... Rosenthal A. (1998)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module. (PubMed id 9303539)1, 2 Salcini A.E....Di Fiore P.P. (1997)
    4. The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport. (PubMed id 10613896)1, 9 Doria M....Di Fiore P.P. (1999)
    5. Intersectin (ITSN) family of scaffolds function as mol ecular hubs in protein interaction networks. (PubMed id 22558309)1 Wong K.A....O'Bryan J.P. (2012)
    6. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    7. Rev-ing up post-transcriptional HIV-1 RNA expression. (PubMed id 21358275)1 Yedavalli V.S. and Jeang K.T. (2011)
    8. Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
    9. Lysine acetylation targets protein complexes and co-regulates major cellular functions. (PubMed id 19608861)2 Choudhary C.... Mann M. (2009)
    10. Cellular proteins and HIV-1 Rev function. (PubMed id 19149558)1 Suhasini M. and Reddy T.R. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 3268 HGNC: 5177 AceView: HRBL Ensembl:ENSG00000106351 euGenes: HUgn3268
    ECgene: AGFG2 H-InvDB: AGFG2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for AGFG2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for AGFG2 gene:
    Search GeneIP for patents involving AGFG2

    GeneCards and IP:
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