AEN Gene
protein-coding GIFtS: 49
GCID: GC15P089164
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apoptosis enhancing nuclease(Previous name: interferon stimulated exonuclease gene 20kDa-like 1 ) (Previous symbol: ISG20L1)
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Aliases for AEN gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Apoptosis Enhancing Nuclease1 2 | | FLJ125621 | | ISG20L11 2 3 5 | | Apoptosis-Enhancing Nuclease2 | | Interferon Stimulated Exonuclease Gene 20kDa-Like 11 2 | | Pp127441 | | Interferon-Stimulated 20 KDa Exonuclease-Like 12 3 | | EC 3.1.-.-3 | | FLJ124841 | | EC 3.1.13.18 |
Export aliases for AEN gene to outside databasesPrevious GC identifers: GC15P086967 GC15P065276 |
Summaries for AEN gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: AEN_HUMAN, Q8WTP8Function: Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis.When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNAdamage signals, leading to enhancement of apoptosis
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Genomic Views for AEN gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000015.9 NC_018926.1 NT_010274.17
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the AEN gene promoter: RFX1 STAT1 Olf-1 E47 HEN1 Pax-3 FOXJ2 (long isoform) Hand1 Chx10 FOXJ2 Other transcription factors
Search SABiosciences Chromatin IP Primers for AEN
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AEN |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 15q26.1 Ensembl cytogenetic band: 15q26.1 HGNC cytogenetic band: 15q26.1AEN Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 15 GeneLoc Exon Structure GeneLoc location for GC15P089164: view genomic region
(about GC identifiers)
Start:
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89,164,527 bp from pter |
End:
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89,175,513 bp from pter |
Size:
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10,987 bases |
Orientation:
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plus strand |
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Proteins for AEN gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: AEN_HUMAN, Q8WTP8 (See
protein sequence)Recommended Name: Apoptosis-enhancing nuclease Size: 325 amino acids; 36350 Da
Subcellular location: Nucleus. Nucleus, nucleolus. Note=Localized predomintly in the nucleolus. Translocates from thenucleolus to the nucleoplasm upon apoptosis induction
Sequence caution: Sequence=AAH14407.1; Type=Erroneous initiation; Note=Translation N-terminally extended;Sequence=BAB14091.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: C9J571 Q9BSA5 Q9H9X7Alternative splicing: 2 isoforms: Q8WTP8-1 Q8WTP8-2 Explore the universe of human proteins at neXtProt for AEN: NX_Q8WTP8
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8WTP8 2 DME Specific Peptides for AEN (Q8WTP8)
AEN Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_073604.3 ENSEMBL proteins: ENSP00000331944 ENSP00000453631 ENSP00000368533 Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
AEN for ontologies About GeneDecksing
AEN Antibody Products: Assay Products for AEN: |
Protein
Domains / Families for AEN gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
AEN for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q8WTP8ProtoNet protein and cluster: Q8WTP8 2 Blocks protein families: IPB006055 Exonuclease IPB013520 Exonuclease
UniProtKB/Swiss-Prot: AEN_HUMAN, Q8WTP8Similarity: Contains 1 exonuclease domain |
Function for AEN gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: AEN_HUMAN, Q8WTP8Function: Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis.When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNAdamage signals, leading to enhancement of apoptosisInduction: Up-regulated by p53/TP53 in response to ionizing radiation and DNA-damaging agents such as adriamycin.Phosphorylation of p53/TP53 at 'Ser-15' is required for effective inductionEnzyme Numbers (IUBMB): EC 3.1.-.-1 EC 3.1.13.12
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for AEN (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AEN (see all 2) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: AEN (NM_022767) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for AEN | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AEN  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AEN |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table
AEN for ontologies About GeneDecksing
|
Pathways & Interactions for AEN gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for AEN
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
 3 Interacting proteins for AEN (Q8WTP83 ENSP000003319444) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006915 | apoptotic process |
-- | -- | | GO:0010212 | response to ionizing radiation |
IDA | 16171785 | | GO:0042771 | intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator |
IDA | 18264133 |
AEN for ontologies About GeneDecksing
|
Drugs & Compounds for AEN gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Browse Tocris compounds for AEN Search CenterWatch for drugs/clinical trials and news about AEN 
|
Transcripts for AEN gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for AEN gene: NM_022767.3 Unigene Cluster for AEN: Apoptosis enhancing nuclease Hs.436102 [show with all ESTs]Unigene Representative Sequence: AB2093237 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000332810(uc002bmt.2 uc010bnm.1) ENST00000557787(uc010bnl.2) ENST00000559528 ENST00000560174 ENST00000558327 ENST00000557927 ENST00000379231
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for AEN (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AEN (see all 2) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: AEN (NM_022767) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for AEN | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AEN  |
Additional cDNA sequence: AB209323.1 AF318330.1 AF327352.1 AK022546.1 AK022624.1 AK128623.1 AK308119.1 BC005164.1 BC014407.1 BC020988.1 11 DOTS entries: DT.75103713 DT.100764611 DT.100785255 DT.100785245 DT.121036988 DT.95073379 DT.99949586 DT.100785254 DT.102843984 DT.97768521 DT.100785256 24/276 AceView cDNA sequences (see all 276): BM801751 BM745692 AI752192 AA227388 BM759943 BQ271083 BM504615 BX283892 BM477244 CB120991 AA969167 BE385033 BG329274 BM977933 BM770493 NM_022767 AI341441 BI820992 BU178063 CD366313 AW070317 AW410818 BQ225954 BG114228 GeneLoc Exon Structure
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Expression for AEN gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| AEN expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: GTGAGCCACA
About this image See AEN Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for AEN
SOURCE GeneReport for Unigene cluster: Hs.436102 SABiosciences Custom PCR Arrays for AEN
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for AEN Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat AEN | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat AEN | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat AEN | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AEN |
Orthologs for AEN gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for AEN gene from 4/17 species (see all 17) About this table
| Organism |
Taxonomic classification |
Gene |
Description |
Human Similarity |
Orthology Type |
Details |
chicken (Gallus gallus) |
Aves |
AEN1 |
apoptosis enhancing nuclease |
64.5(n) 58.12(a) |
  |
415496 XM_413869.3 XP_413869.3 |
lizard (Anolis carolinensis) |
Reptilia |
AEN6 |
-- |
37(a) |
1 ↔ 1 |
GL343844.1(143338-149575) |
African clawed frog (Xenopus laevis) |
Amphibia |
CB944151.12 |
-- |
73.69(n) |
  |
CB944151.1 |
zebrafish (Danio rerio) |
Actinopterygii |
BX294110.16 |
-- |
31(a) |
possible ortholog |
25(19586822-19590539) |
ENSEMBL Gene Tree for AEN (if available) TreeFam Gene Tree for AEN (if available)  |
Paralogs for AEN gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for AEN gene
- ISG202 ISG20L22 REXO42
3 SIMAP similar genes for AEN using alignment to 3 protein entries: AEN_HUMAN (see all proteins):pp12744 ISG20 ISG20L2
AEN for paralogs About GeneDecksing
|
Genomic Variants for AEN gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 15 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
|---|
HapMap Linkage Disequilibrium report for AEN (89164527 - 89175513 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for AEN: --  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing AEN |
|
Disorders
/ Diseases for AEN gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
AEN for disorders About GeneDecksing
OMIM gene information: 610177
OMIM disorders: --
1 disease for AEN: About MalaCardsgastric outlet obstruction 1 disease from the University of Copenhagen DISEASES database for AEN:Liver neoplasm Export disorders for AEN gene to outside databases
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Publications for AEN gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for AEN gene, integrated from 9 sources (see all 12): (articles sorted by number of sources associating them with AEN) | |  | Utopia: connect your pdf to the dynamic world of online information |
- p53 target gene AEN is a nuclear exonuclease required for p53-dependent apoptosis. (PubMed id 18264133)1, 2, 3 Kawase T....Taya Y. (2008)
- Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis. (PubMed id 16171785)1, 2, 3 Lee J.H....Bae S. (2005)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- ISG20L1 is a p53 family target gene that modulates ge notoxic stress-induced autophagy. (PubMed id 20429933)1 Eby K.G....Pietenpol J.A. (2010)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (2007)
- Analysis of the DNA sequence and duplication history of human chromosome 15. (PubMed id 16572171)2 Zody M.C....Nusbaum C. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
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External Searches for AEN gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing AEN gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing AEN gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing AEN gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for AEN | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for AEN gene: Search GeneIP for patents involving AEN
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for AEN gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
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| | | | OriGene Antibodies for AEN | | OriGene shRNA RFP for AEN | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for AEN | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for AEN | | OriGene Protein Over-expression Lysate for AEN | | Browse OriGene Fluorogenic Cell Assay Kits | | OriGene siRNA for AEN | | Browse 3'-UTR reporter clones for miRNA target validation | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for AEN | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for AEN | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | OriGene Purified Protein for AEN | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | OriGene Custom Antibody Services for AEN | | OriGene Custom Protein Services for AEN | | OriGene Custom Immunoassay Development | | |
| |  |
 |
| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat AEN | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing AEN | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AEN | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat AEN | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat AEN | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat AEN |
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| | | Search Tocris compounds for AEN |
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 | | AEN Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AEN |
|  |  |  | | | ThermoFisher Antibodies for AEN |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat AEN |
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