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ADM2 Gene

protein-coding GIFtS: 44

GC22P049267

adrenomedullin 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
AM2 ^¹, ², ³
FLJ21135 ^¹, ²
Intermedin ^³
OTTHUMP00000028752 ^²
OTTHUMP00000196596 ^²
dJ579N16.4 ^²
intermedin ^²

Descriptions
adrenomedullin 2 ^²

External Ids
HGNC: 28898¹
Entrez Gene: 79924²
UniProtKB: Q7Z4H4³
Ensembl: ENSG00000128165⁷

Search outside databases for aliases for ADM2 gene

Previous GC identifers: GC22P049053 GC22P049055 GC22P049210

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ADM2:

ADM2 belongs to a family of calcitonin (MIM 114130)-related peptide hormones important for

regulating diverse physiologic functions and the chemical composition of fluids and

tissues.[supplied by OMIM]

UniProtKB/Swiss-Prot: ADM2_HUMAN, Q7Z4H4

Function: IMDL and IMDS may play a role as physiological regulators of gastrointestinal,

cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the

cAMP-dependent pathway

Gene Wiki entry for ADM2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the ADM2 gene

Entrez Gene cytogenetic band: 22q13.33 Ensembl cytogenetic band: 22q13.33 HGNC cytogenetic band: 22q13.33

ADM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 22 GeneLoc Exon Structure

GeneLoc location for GC22P049267: (about GC identifiers)

Start:	49,266,878 bp from pter
End:	49,271,732 bp from pter
Size:	4,855 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000022.9 NT_011526.6

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ADM2_HUMAN, Q7Z4H4 (See protein sequence)

Recommended Name: ADM2 precursor

Size: 148 amino acids; 15865 Da

Subcellular location: Secreted

Secondary accessions: Q3LFQ0

REFSEQ proteins: NP_079142.2

ENSEMBL proteins:

ENSP00000379087 ENSP00000354955 ENSP00000379086

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--

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Antibodies for ADM2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for ADM2

Assays for ADM2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q7Z4H4

ProtoNet protein and cluster: Q7Z4H4

UniProtKB/Swiss-Prot: ADM2_HUMAN, Q7Z4H4

Similarity: Belongs to the adrenomedullin family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ADM2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024866

Applied Biosystems Silencer® siRNAs for ADM2

Sigma-Aldrich siRNA for ADM2

Sigma-Aldrich shRNA for ADM2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_024866
Myc/DDK tagged cDNA clone in CMV expression vector: NM_024866
untagged cDNA clone in CMV expression vector: NM_024866

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_024866

UniProtKB/Swiss-Prot: ADM2_HUMAN, Q7Z4H4

Function: IMDL and IMDS may play a role as physiological regulators of gastrointestinal,

cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the

cAMP-dependent pathway

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005179	hormone activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

5/10 Interacting proteins for ADM2 (ENSP00000354955³) via UniProtKB, MINT, and/or STRING (see all 10 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CALCA	ENSP00000331746³	STRING (score=.859)
RAB27A	ENSP00000337761³	STRING (score=.808)
ADM	ENSP00000278175³	STRING (score=.75)
RAMP2	ENSP00000253796³	STRING (score=.75)
RAMP3	ENSP00000242249³	STRING (score=.75)

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ADM2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ADM2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024866

Sigma-Aldrich siRNA for ADM2

Sigma-Aldrich shRNA for ADM2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_024866

REFSEQ mRNAs for ADM2 gene:

NM_024866.4

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_024866

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024866
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024866
                                 untagged cDNA clone in CMV expression vector: NM_024866

Additional cDNA sequence:

AB121034.1 AB236970.1 AF529213.1 AK024788.1 AK090635.1 AK092640.1 AK225097.1 BC012864.1

CR456479.1

6 DOTS entries:

DT.308897 DT.120661663 DT.70105009 DT.439114 DT.100704709 DT.91833961

24/35 AceView cDNA sequences (see all 35 ):

CR456479 NM_024866 AW445022 AB121034 AK092640 CN481163 AA345985 AK024788

AI650897 AA962212 AK090635 BX400262 AI638686 BU630265 BU584945 BC012864

AA999846 AF529213 AI633839 CA430647 AA151693 AA316865 BM910446 BX348470

highest scoring ESTs for ADM2:

AA999846 AI638686 AA099471 AA149567 AA151693 AA316865 AA345985 AA918415 AA962212 AB121034

Unigene Cluster for ADM2:

Adrenomedullin 2

Hs.449099 [show with all ESTs]

Unigene Representative Sequence: NM_024866

GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:

ENST00000395738 ENST00000362068 ENST00000395737

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ADM2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for ADM2

¹ / ² / ³

4 probe-sets matching ADM2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
69327_at²^{, 3}	U95-E	1	1.00	1.00	0.72	1.14	AI638686	1.00	1.00	1.00	1
47856_at²^{, 3}	U95-B	1	1.00	1.00	0.97	0.87	AA999846	1.00	1.00	1.00	1
220538_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_024866	0.60	1.00	0.82	1
220538_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks ADM2 for binary patterns associated with the probe-sets selected above

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Data from

(Publications) and GNF BioGPS
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CGAP SAGE TAG: GGAAGTTGCG

SOURCE GeneReport for Unigene cluster: Hs.449099
UniProtKB/Swiss-Prot: ADM2_HUMAN, Q7Z4H4
Tissue specificity: Expressed in the esophagus, stomach, jejunum, ileum, ileocecum, ascending
colon, transverse colon, descending colon and rectum. Expressed in myocardial cells of the heart,
renal tubular cells, hypothalamus, and pituitary

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for ADM2 gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	ADM2¹	--	adrenomedullin 2	80.28(n) 71.13(a)	606919 XM_843399.1 XP_848492.1
chimpanzee (Pan troglodytes)	ADM2¹	--	adrenomedullin 2	99.32(n) 97.97(a)	470251 XM_001141651.1 XP_001141651.1
cow (Bos taurus)	ADM2¹	--	adrenomedullin 2	80.99(n) 71.13(a)	618896 XM_871213.1 XP_876306.1
rat (Rattus norvegicus)	Adm2¹	--	adrenomedullin 2	75.06(n) 66.19(a)	399475 NM_201426.1 NP_958829.1
mouse (Mus musculus)	Adm2^{1, 5}	15⁵	adrenomedullin 2^{1, 5}	73.24(n)¹ 66.2(a)¹	223780¹ NM_182928.3¹ NP_891558.1¹ AA692917⁵ AB121035⁵ (see all 8)

About this table Species with no ortholog for ADM2

ENSEMBL Gene Tree for ADM2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/36 NCBI SNPs in ADM2 are shown (see all 36 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 49)

AB	SNP ID	Valid	Chr 22 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs9616854^1,2	A,C,F,H	49265272(+)	`CGAACG/AGACGC`	1	--	ng5¹		4		EU EA WA	420
	rs876278^1,2	A,F,H,O	49269908(-)	`GTAGCG/CGACGC`	1	--	ut3¹ ese³		3		EA WA	296
	rs2236031^1,2	A,C,F,H	49268560(+)	`CTGAAC/TTGGCC`	1	--	ut3¹		8		EU EA WA	838
	rs8141795^1,2	A,H	49268668(+)	`TGCCTG/CTGGGA`	1	--	ut3¹ ese³		4		EU EA WA	414
	rs2236032^1,2	F,H	49269336(+)	`GGGTCG/ACCATG`	1	--	ut3¹ ese³		5		EA EU WA	1884
	rs13055726^1,2	C,F	49269410(+)	`GTTCCT/CACCTC`	1	--	ut3¹ ese³		2		NA	182
	rs13056677^1,2	F,H	49268510(+)	`GCTGGG/ACCCTG`	1	--	ut3¹ ese³		4		EU EA WA	400
--	rs8136917^1,2	A	49268844(+)	`AGGGGA/GACTCA`	1	--	ut3¹ ese³ trp³		0	--	--	--	--
--	rs8136908^1,2	A	49268827(+)	`CCTGTA/GGGAGG`	1	--	ut3¹		0	--	--	--	--
	rs13053471^1,2	C	49269979(+)	`CACCCC/TGTCTG`	1	--	ut3¹ ese³		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for ADM2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 608682

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/32 PubMed articles for ADM2 gene (see all 32 ):

Identification of novel adrenomedullin in mammals: a potent cardiovascular and renal regulator. (PubMed id 14706825)^{1, 2, 3, 4} Takei Y.... Miyano S. (2004)
Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney. (PubMed id 16359754)^{1, 3, 4} Takahashi K....Totsune K. (2006)
Intermedin is a calcitonin/calcitonin gene-related peptide family peptide acting through the calcitonin receptor-like receptor/receptor activity-modifying protein receptor complexes. (PubMed id 14615490)^{3, 4} Roh J.... Hsu S.Y.T. (2004)
The DNA sequence of human chromosome 22. (PubMed id 10591208)^{3, 4} Dunham I....O'Brien K.P. (1999)
Intermedin (adrenomedullin-2): a novel counter-regulatory peptide in the cardiovascular and renal systems. (PubMed id 17965749)^{1, 3} Bell D. and McDermott B.J. (2008)
Intermedin: a skin peptide that is downregulated in atopic dermatitis. (PubMed id 17008878)^{1, 3} Kindt F....Haberberger R.V. (2007)
Intermedin ameliorates vascular and renal injury by inhibition of oxidative stress. (PubMed id 18829738)^{1, 3} Hagiwara M....Chao J. (2008)
Expression of adrenomedullin2/intermedin in human brain, heart, and kidney. (PubMed id 17346853)^{1, 3} Morimoto R....Takahashi K. (2007)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)³ Ota T....Sugano S. (2004)
A genome annotation-driven approach to cloning the human ORFeome. (PubMed id 15461802)³ Collins J.E.... Dunham I. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 79924	HGNC: 28898	AceView: ADM2	Ensembl:ENSG00000128165	euGenes: HUgn79924
ECgene: ADM2	H-InvDB: ADM2

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for ADM2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for ADM2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for ADM2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for ADM2		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for ADM2		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Cell Culture Products		ELISAs
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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ADM2 Gene

protein-coding GIFtS: 44

Aliases &
Descriptions
for ADM2

Summaries
for ADM2

Genomic Location
for ADM2

Proteins
for ADM2

Protein Domains/
Families
for ADM2

Gene Function
for ADM2

Pathways & Interactions
for ADM2

Drugs & Compounds
for ADM2

Transcripts
for ADM2

Expression
for ADM2

Orthologs
for ADM2

Paralogs
for ADM2

SNPs/Variants
for ADM2

Disorders & Mutations
for ADM2

Medical News
for ADM2

Publications
for ADM2

Search for ADM2

Genome Databases showing ADM2

Other Databases showing ADM2

Specialized Databases showing ADM2

Licensable Technologies
for ADM2

Services
for ADM2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

ADM2 Gene

protein-coding GIFtS: 44

Aliases & Descriptionsfor ADM2

Summariesfor ADM2

Genomic Locationfor ADM2

Proteinsfor ADM2

Protein Domains/Familiesfor ADM2

Gene Functionfor ADM2

Pathways & Interactionsfor ADM2

Drugs & Compoundsfor ADM2

Transcriptsfor ADM2

Expression for ADM2

Orthologsfor ADM2

Paralogsfor ADM2

SNPs/Variantsfor ADM2

Disorders & Mutationsfor ADM2

Medical Newsfor ADM2

Publicationsfor ADM2

Search for ADM2

Genome Databases showing ADM2

Other Databases showing ADM2

Specialized Databases showing ADM2

Licensable Technologiesfor ADM2

Servicesfor ADM2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for ADM2

Summaries
for ADM2

Genomic Location
for ADM2

Proteins
for ADM2

Protein Domains/
Families
for ADM2

Gene Function
for ADM2

Pathways & Interactions
for ADM2

Drugs & Compounds
for ADM2

Transcripts
for ADM2

Expression
for ADM2

Orthologs
for ADM2

Paralogs
for ADM2

SNPs/Variants
for ADM2

Disorders & Mutations
for ADM2

Medical News
for ADM2

Publications
for ADM2

Licensable Technologies
for ADM2

Services
for ADM2