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ADI1 Gene

protein-coding   GIFtS: 54

GC02M003480
acireductone dioxygenase 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
APL1 1, 2
ARD 1, 2, 3
EC 1.13.-.- 3
FLJ10913 1, 2
HMFT1638 1, 2
MTCBP-1 1, 2, 3
MTCBP1 2, 3
SIP-L 2
SIPL 1, 2, 3
Descriptions
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2
Aci-reductone dioxygenase 3
MT1-MMP cytoplasmic tail-binding protein-1 2
Membrane-type 1 matrix metalloproteinase cytoplasmic
tail-binding protein 1 3
Submergence-induced protein 2 homolog 3
acireductone dioxygenase 1 2
membrane-type 1 matrix metalloproteinase cytoplasmic tail
binding protein-1 2
submergence induced protein 2 2
External Ids
HGNC: 305761
Entrez Gene: 552562
UniProtKB: Q9BV573
Ensembl: ENSG000001825517
Search outside databases for aliases for ADI1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ADI1:
This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding
enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the
nucleus, and may carry out different functions depending on its localization. A pseudogene that is
located on chromosome 20 has been defined for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: MTND_HUMAN, Q9BV57
Function: Has aci-reductone dioxygenase (ARD) activity and can function in the
5-methylthioadenosine (MTA) methionine salvage pathway. Down-regulates cell migration mediated by
MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line

Gene Wiki entry for ADI1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ADI1 gene  

Entrez Gene cytogenetic band: 2p25.3   Ensembl cytogenetic band:  2p25.3   HGNC cytogenetic band: 2p25.2

ADI1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M003480:     (about GC identifiers)

Start:
3,480,698 bp from pter
End:
3,502,306 bp from pter
Size:
21,609 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_022221.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MTND_HUMAN, Q9BV57 (See protein sequence)
Recommended Name: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase  
Size: 179 amino acids; 21498 Da
Cofactor: Binds 1 nickel ion per monomer. Can also use other divalent metal cations (By similarity)
Subunit: Interacts with MMP14
Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus
Secondary accessions: Q53HW3 Q53QD3 Q57YV7 Q68CK2 Q6ZSF7 Q7Z512 Q96P85 Q9NV57
Alternative splicing: 2 isoforms:  Q9BV57-1   Q9BV57-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_060739.2  

    ENSEMBL proteins: 
    ENSP00000371525 ENSP00000333666 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (ADI1)
    Human Recombinant Proteins from Abnova (MTCBP-1)
                  OriGene Purified Recombinant Human Protein: ADI1 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA11602742 14718544
    GO:0005737 cytoplasm IDA11602742 14718544
    GO:0005886 plasma membrane IDA14718544
    About this table

    Antibodies for ADI1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for ADI1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (ADI1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (MTCBP-1)
    Novus Biologicals Antibodies for ADI1

    Assays for ADI1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR004313 Acireductn_dOase
     IPR014710 RmlC-like_jellyroll


       GeneDecks  ADI1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9BV57

    ProtoNet protein and cluster: Q9BV57

    UniProtKB/Swiss-Prot: MTND_HUMAN, Q9BV57
    Similarity: Belongs to the acireductone dioxygenase (ARD) family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ADI1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018269

                  Applied Biosystems Silencer® siRNAs for ADI1

                  Sigma-Aldrich siRNA for ADI1  
                         Sigma-Aldrich shRNA for ADI1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for ADI1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018269
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018269
                                     untagged cDNA clone in CMV expression vector: NM_018269 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018269

    UniProtKB/Swiss-Prot: MTND_HUMAN, Q9BV57
    Function: Has aci-reductone dioxygenase (ARD) activity and can function in the
    5-methylthioadenosine (MTA) methionine salvage pathway. Down-regulates cell migration mediated by
    MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line
    Enzyme Number (IUBMB): EC 1.13.-.- 

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI14718544
    GO:0016151 nickel ion binding IEA--
    GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  ADI1  (Your Favorite Gene powered by Ingenuity) 
     Inositol Metabolism

       GeneDecks  ADI1 for the pathways selected above  
    About GeneDecksing

    UniProtKB/Swiss-Prot: MTND_HUMAN, Q9BV57
    Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from
    (S)-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6

     Gene Network CentralTM Interacting Genes and Proteins Network for  ADI1 


    5/6 Interacting proteins for ADI1 (ENSP000003336663 Q9BV571) via UniProtKB, MINT, and/or STRING (see all 6 )

    InteractantInteraction Details
    GeneCardExternal ID(s)
    MMP14P502811STRING (score=.779) EBI-992807, EBI-992788
    OSTF1Q928821EBI-992807, EBI-1051152
    CASRENSP000003535563STRING (score=.907)
    ENOPH1ENSP000002739203STRING (score=.844)
    APIPENSP000002783593STRING (score=.809)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008652 amino acid biosynthetic process IEA--
    GO:0009086 methionine biosynthetic process IEA--
    GO:0019509 methionine salvage IDA15938715
    GO:0055114 oxidation reduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ADI1
    5 Novoseek chemical compound relationships for ADI1 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    methionine 52.76 7 17786183 (1), 18237192 (1), 17212658 (1), 14718544 (1)
    carboxylate 51.46 3 17288413 (2), 17645333 (1)
    fe2+ 51.32 4 16989860 (3)
    oxygen 18.36 3 18237192 (1)
    hydrogen 11.07 2 17645333 (1), 17288413 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ADI1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018269

                  Sigma-Aldrich siRNA for ADI1  
                         Sigma-Aldrich shRNA for ADI1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018269  

    REFSEQ mRNAs for ADI1 gene: 

    NM_018269.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018269  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018269
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018269
                                     untagged cDNA clone in CMV expression vector: NM_018269 

    Additional cDNA sequence: 

    AB073609.1 AB158319.1 AF087863.1 AF403478.1 AK001775.1 AK127473.1 AK222467.1 AK225278.1 
    BC001467.2 CR592882.1 CR593705.1 CR595221.1 CR595303.1 CR595663.1 CR598608.1 CR602689.1 
    CR603459.1 CR604038.1 CR604779.1 CR605242.1 CR607574.1 CR609057.1 CR611145.1 CR617197.1 
    CR624357.1 CR624573.1 CR625343.1 

    20 DOTS entries:

    DT.446852  DT.99956085  DT.101985071  DT.92451152  DT.99988886  DT.100827485  DT.100827486  DT.100827475 
    DT.100827480  DT.97794559  DT.100700644  DT.100827474  DT.100827495  DT.91832014  DT.92451148  DT.92451149 
    DT.95256744  DT.95256759  DT.100827487  DT.75162181 

    24/493 AceView cDNA sequences (see all 493 ):

    BM718092 R24642 AA693413 BG776692 BI545934 AA485906 AA360213 BU508179 
    BU621255 AA554338 AA314427 BQ639872 CA310252 AI476741 AI458820 CR624573 
    AI186551 CR625343 AA845713 BE855740 T34519 CB161069 BM838044 CR605242 

    highest scoring ESTs for ADI1:

    AA425430 AB158319 AF087863 AK001775 AL528742 AL543802 AL546607 AL547323 AL547745 AL549060 

    Unigene Cluster for ADI1:

    Acireductone dioxygenase 1
    Hs.502773  [show with all ESTs]
    Unigene Representative Sequence: AK127473


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for ADI1

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b · 6c
    SP1:        -                             -                     
    SP2:                                                            
    SP3:                                -     -                     
    SP4:                                                            

    About this scheme

    ECgene alternative splicing isoforms for ADI1

    2 Ensembl transcripts including schematic representations:
    ENST00000382093  ENST00000327435  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ADI1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ADI1

    1 / 2 / 3

    7 probe-sets matching ADI1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    57203_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA142864 0.60 1.00 0.82 1

    222400_s_at2, 3 U133-B 1 1.00 1.00 -- -- BC001467 0.80 1.00 0.91 1

    217761_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018269 0.60 1.00 0.82 1

    214019_at2 U133-A 2 0.36 0.88 -- -- -- -- -- -- --

    222400_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    217761_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    214019_at2 U133Plus2 2 0.36 0.88 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCTTGGAGTT

    SOURCE GeneReport for Unigene cluster: Hs.502773

    UniProtKB/Swiss-Prot: MTND_HUMAN, Q9BV57
    Tissue specificity: Detected in heart, colon, lung, stomach, brain, spleen, liver, skeletal muscle
    and kidney

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ADI1 gene from 5/17 species (see all 17 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    ADI11   -- acireductone dioxygenase 1 98.88(n)
    98.88(a)
    743384  XM_001153347.1  XP_001153347.1 
    cow
    (Bos taurus)
    ADI11   -- acireductone dioxygenase 1 84.36(n)
    84.92(a)
    615764  NM_001035468.1  NP_001030545.1 
    rat
    (Rattus norvegicus)
    Adi11   -- acireductone dioxygenase 1 83.61(n)
    84.27(a)
    298934  NM_199097.1  NP_954528.1 
    mouse
    (Mus musculus)
    Adi11, 5 125
    acireductone dioxygenase 11, 5 84.17(n)1
    83.8(a)1
    1049231  NM_134052.21  NP_598813.11 
     AK0899665  AK1458315  (see all 9)
    chicken
    (Gallus gallus)
    RCJMB04_7o81   -- acireductone dioxygenase 1 72.63(n)
    78.21(a)
    421918  NM_001031089.1  NP_001026260.1 
    About this table        Species with no ortholog for ADI1

    ENSEMBL Gene Tree for ADI1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Pseudogenes
    2 related pseudogenes


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/185 NCBI SNPs in ADI1 are shown (see all 185 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 62)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs33491,2
    A,C,F,H3481205(+) CTGAAA/TATAAG 1 -- ut31 ese39Minor allele frequency- N:0.00MN EU EA WA NA 806
    rs101793401,2
    A,C,F,H3502328(+) GCTTTG/ATCTTA 1 -- ut51 ese34Minor allele frequency- A:0.24EU EA WA 418
    --
    rs11301651,2
    A,C,F,H3481405(-) CTGCCG/ATGTAA 1 -- ut31 ese35Minor allele frequency- A:0.26MN EU EA WA 596
    rs11301271,2
    A,C,F,H3481632(-) GCTAGA/GTTATC 1 -- ut319Minor allele frequency- G:0.29MN EU EA WA NA 926
    rs11303331,2
    C,F,H3481042(-) TAACAG/AGAGTA 1 -- ut31 ese310Minor allele frequency- A:0.28NS EU EA WA MN 696
    rs11303191,2
    C,F3481062(-) ACAATA/CAAGCT 1 -- ut311Minor allele frequency- C:0.15MN 184
    rs102084941,2
    C,F3481716(+) GAGGCA/GCGTGT 1 -- ut31 ese31Minor allele frequency- G:0.19MN 184
    --
    rs67513501,2
    C,F3502448(+) AGCCCC/AGACCC 1 -- ng312Minor allele frequency- A:0.22MN EA 1252
    --
    rs33501,2
    C3481315(+) TTCCCA/CCTACC 1 -- ut31 trp30--------
    --
    rs101825251,2
    C3503233(+) TCCTTC/GAAGAA 1 -- ng31 tfbs30--------
    About this table

    HapMap Linkage Disequilibrium images for ADI1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/21 PubMed articles for ADI1 gene (see all 21 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55256 HGNC: 30576 AceView: MTCBP-1 Ensembl:ENSG00000182551 euGenes: HUgn55256
    ECgene: ADI1 H-InvDB: ADI1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ADI1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for ADI1 Antibodies for ADI1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ADI1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for ADI1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for ADI1

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