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ADFP Gene

protein-coding   GIFtS: 56

GC09M019105
adipose differentiation-related protein
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
ADRP 1, 2, 3
MGC10598 2
OTTHUMP00000021107 2
adipophilin 2
Descriptions
adipose differentiation-related protein 2, 3
External Ids
HGNC: 2481
Entrez Gene: 1232
UniProtKB: Q995413
Ensembl: ENSG000001478727
Search outside databases for aliases for ADFP gene

Previous GC identifers: GC09M019445 GC09M019304 GC09M019098

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for ADFP:
Adipocyte differentiation-related protein is associated with the globule surface membrane material.
This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the
earliest indications of adipocyte differentiation [provided by RefSeq]

UniProtKB/Swiss-Prot: ADFP_HUMAN, Q99541
Function: May be involved in development and maintenance of adipose tissue (By similarity)

Gene Wiki entry for ADFP

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ADFP gene  

Entrez Gene cytogenetic band: 9p22.1   Ensembl cytogenetic band:  9p22.1   HGNC cytogenetic band: 9p22.1

ADFP Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09M019105:     (about GC identifiers)

Start:
19,105,759 bp from pter
End:
19,117,573 bp from pter
Size:
11,815 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000009.10  NT_008413.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ADFP_HUMAN, Q99541 (See protein sequence)
Recommended Name: Adipophilin  
Size: 437 amino acids; 48075 Da
Subcellular location: Membrane; Peripheral membrane protein
Secondary accessions: Q9BSC3

Post-translational modifications:

  • Acylated; primarily with C14, C16 and C18 fatty acids1


  • REFSEQ proteins: NP_001113.2  

    ENSEMBL proteins: 
    ENSP00000369832 ENSP00000369831 ENSP00000276914 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (ADFP)
    Human Recombinant Proteins from Abnova (ADFP)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region TAS9003395
    GO:0005634 nucleus IEA--
    GO:0005737 cytoplasm IEA--
    GO:0005783 endoplasmic reticulum TAS9003395
    GO:0005811 lipid particle TAS9003395
    About this table

    Antibodies for ADFP: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of ADFP
    Sigma-Aldrich Antibodies for ADFP
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (ADFP), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (ADFP)
    Novus Biologicals Antibodies for ADFP

    Assays for ADFP: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR004279 Perilipin


       GeneDecks  ADFP for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q99541

    ProtoNet protein and cluster: Q99541

    1 Blocks protein family: IPB004279 Perilipin

    UniProtKB/Swiss-Prot: ADFP_HUMAN, Q99541
    Similarity: Belongs to the perilipin family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ADFP)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001122

                  Applied Biosystems Silencer® siRNAs for ADFP

                  Sigma-Aldrich siRNA for ADFP  
                         Sigma-Aldrich shRNA for ADFP  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for ADFP
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001122
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001122
                                     untagged cDNA clone in CMV expression vector: NM_001122 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001122

    UniProtKB/Swiss-Prot: ADFP_HUMAN, Q99541
    Function: May be involved in development and maintenance of adipose tissue (By similarity)

    6 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Adfp):

    adipose tissueendocrine/exocrine glandhomeostasis/metabolismliver/biliary systemreproductive system
    vision/eye

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  ADFP 


    5/31 Interacting proteins for ADFP (ENSP000002769143) via UniProtKB, MINT, and/or STRING (see all 31 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RHOENSP000002962713STRING (score=.951)
    PRPF8ENSP000003043503STRING (score=.945)
    CRXENSP000002219963STRING (score=.933)
    NRLENSP000003370233STRING (score=.928)
    PRPHENSP000002578603STRING (score=.923)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0015909 long-chain fatty acid transport IEA--
    GO:0019915 lipid storage IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ADFP
    10/11 Novoseek chemical compound relationships for ADFP gene (see all 11 )
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    lipid 72.37 207 9392423 (8), 14707038 (6), 17872589 (6), 12804567 (5) (see all 74)
    triacylglycerol 61.61 11 16230742 (4), 17872589 (2), 16115879 (1)
    oleic acid 58.84 10 16230742 (2), 16115879 (2), 12210744 (1), 16391323 (1) (see all 5)
    acyl-coa 54.90 1 12893739 (1)
    fatty acid 46.93 20 14671211 (2), 16260216 (2), 16391323 (2), 16115879 (2) (see all 12)
    mannose 6-phosphate 43.27 1 17872589 (1)
    sterol 39.03 4 10954705 (1), 17671740 (1), 18192234 (1)
    cholesterol 29.86 17 17446422 (3), 14707038 (3), 11333258 (2), 10954705 (1) (see all 9)
    prostaglandin 10.32 5 15689536 (2), 15657374 (2)
    arachidonic acid 3.76 2 15689536 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ADFP)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001122

                  Sigma-Aldrich siRNA for ADFP  
                         Sigma-Aldrich shRNA for ADFP  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001122  

    REFSEQ mRNAs for ADFP gene: 

    NM_001122.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001122  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001122
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001122
                                     untagged cDNA clone in CMV expression vector: NM_001122 

    Additional cDNA sequence: 

    AF443203.1 AK022061.1 AK094196.1 AK296125.1 AK297920.1 AK312983.1 BC005127.2 CR536541.1 
    CR592339.1 CR597463.1 CR600009.1 CR600234.1 CR604128.1 CR605429.1 CR615308.1 CR618768.1 
    CR620358.1 CR624951.1 X97324.1 

    24/33 DOTS entries (see all 33 ):

    DT.91762044  DT.117107  DT.121149566  DT.100667972  DT.121149913  DT.91673230  DT.91762036  DT.95257923 
    DT.100771091  DT.121149936  DT.100765283  DT.91653525  DT.99927880  DT.100054457  DT.121149922  DT.100667971 
    DT.121149708  DT.121149740  DT.95257919  DT.97806939  DT.100059474  DT.100642377  DT.95156312  DT.100667967 

    24/453 AceView cDNA sequences (see all 453 ):

    BG576392 AI961089 BQ648372 BQ012064 BQ029253 BM905751 CR600234 AU133301 
    BM698704 BQ018760 BE733305 BM670795 BX108578 BI494696 BQ646820 BQ012135 
    BQ646549 BM999263 AA775306 BQ653092 BQ649228 BQ649403 AU121155 CR597463 

    highest scoring ESTs for ADFP:

    AA298077 AA298959 AL523948 AL531205 AL531450 AL531886 AL532099 AL553023 AL557906 AL603047 

    Unigene Cluster for ADFP:

    Adipose differentiation-related protein
    Hs.3416  [show with all ESTs]
    Unigene Representative Sequence: NM_001122


    GeneLoc Exon Structure

    5/13 Alternative Splicing Database (ASD) splice patterns (SP) for ADFP (see all 13 )

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2a · 2b ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b · 6c · 6d · 6e ^ 7a · 7b ^ 8 ^ 9
    SP1:                                -     -     -                 -     -     -                 -     -                                                         
    SP2:                                -     -     -           -     -     -     -                 -     -                                                         
    SP3:                                -     -     -                 -     -     -                                                                                 
    SP4:                                -     -     -                 -     -     -                 -     -                                                         
    SP5:                                      -     -                 -     -     -                 -     -                                                         

    About this scheme

    ECgene alternative splicing isoforms for ADFP

    3 Ensembl transcripts including schematic representations:
    ENST00000380465  ENST00000380464  ENST00000276914  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ADFP expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ADFP

    1 / 2 / 3

    3 probe-sets matching ADFP gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    34378_at2, 3 U95-A 4 1.00 0.86 1.00 1.00 X97324 0.80 1.00 0.91 1

    209122_at2, 3 U133-A 2 1.00 0.95 -- -- BC005127 0.80 1.00 0.91 1

    209122_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
    GeneDecks  ADFP for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATGCTTGCTT

    SOURCE GeneReport for Unigene cluster: Hs.3416

    Expression variation in blood from EXPOLDB for ADFP

    UniProtKB/Swiss-Prot: ADFP_HUMAN, Q99541
    Tissue specificity: Milk lipid globules

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ADFP gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ADFP1   -- adipose differentiation-related protein 90.37(n)
    89.74(a)
    474720  XM_859677.1  XP_864770.1 
    chimpanzee
    (Pan troglodytes)
    ADFP1   -- adipose differentiation-related protein 98.35(n)
    98.02(a)
    465012  XM_520505.2  XP_520505.2 
    cow
    (Bos taurus)
    ADFP1   -- adipose differentiation-related protein 88.78(n)
    88.07(a)
    280981  NM_173980.2  NP_776405.1 
    rat
    (Rattus norvegicus)
    Adfp1   -- adipose differentiation related protein 83.45(n)
    81.71(a)
    298199  NM_001007144.1  NP_001007145.1 
    mouse
    (Mus musculus)
    Adfp1, 5 4 (38.90 cM)5
    adipose differentiation related protein1, 5 84.4(n)1
    84.16(a)1
    115201  NM_007408.31  NP_031434.31 
     AA4071575  AK0029115  (see all 33)
    About this table        Species with no ortholog for ADFP

    ENSEMBL Gene Tree for ADFP
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ADFP gene
    LSDP52  PLIN2  M6PRBP12  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/99 NCBI SNPs in ADFP are shown (see all 99 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 29)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 9 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs38086571,2
    C,F,H19117877(-) GCCGGC/ACTGGG 1 -- ng315Minor allele frequency- A:0.33MN EA WA 652
    rs38243681,2
    C,F,H19117902(-) TGTTTG/CCATTT 1 -- ng31 tfbs31Minor allele frequency- C:0.38EU 120
    --
    rs21305721,2
    A,C,F19117665(-) TCTCAC/ACCCGA 1 -- ng31 tfbs32Minor allele frequency- A:0.35MN EA 356
    rs21305731,2
    A,C,F19117646(-) CCACCC/ACTCTA 1 -- ng312Minor allele frequency- A:0.35MN EA 356
    --
    rs78675071,2
    A,C19119432(+) ACCCAG/ATTTCT 1 -- ng313Minor allele frequency- A:0.01NA 142
    --
    rs38086601,2
    C,F19117489(-) CCACGG/CTTTGA 1 -- ut51 ese35Minor allele frequency- C:0.47NA MN EA 496
    --
    rs126868101,2
    A,C19118393(+) ctgtaC/Gtccca 1 -- ng310--------
    rs37802201,2
    H19108390(-) ATAAGC/TTCTAC 1 L/F mis14Minor allele frequency- T:0.00EU EA WA 418
    rs10429361,2
    A19105802(-) GAATAC/TTACCT 1 -- ut31 ese30--------
    --
    rs78532801,2
    C19119142(+) ttgttG/Ttttga 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for ADFP (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 103195

    8 Novoseek disease relationships for ADFP gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    fatty liver 38.78 4 16403437 (3), 11397807 (1)
    atherosclerotic plaque 38.42 4 15961705 (2), 17620659 (1)
    carcinoma renal cell 17.93 3 17200350 (1), 15682440 (1)
    atherosclerosis 6.10 1 18854426 (1)
    necrosis 0.00 1 18037904 (1)
    obesity 0.00 1 17353663 (1)
    carcinoma 0.00 1 19120764 (1)
    tumors 0.00 7 17284252 (1), 17545631 (1), 18688642 (1), 18037904 (1) (see all 5)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/131 PubMed articles for ADFP gene (see all 131 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 123 HGNC: 248 AceView: ADFP Ensembl:ENSG00000147872 euGenes: HUgn123
    ECgene: ADFP H-InvDB: ADFP
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ADFP:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for ADFP Antibodies for ADFP
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ADFP Browse small molecules at Sigma-Aldrich
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