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ADAMTS9 Gene

protein-coding   GIFtS: 56

GC03M064476
ADAM metallopeptidase with thrombospondin type 1 motif, 9
(Previous names: a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 9 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
ADAM-TS9 3
ADAMTS-9 3
EC 3.4.24.- 3
FLJ42955 2
KIAA1312 1, 2, 3
Descriptions
ADAM metallopeptidase with thrombospondin type 1 motif, 9 2
ADAM-TS 9 3
a disintegrin-like and metalloprotease (reprolysin type)
with thrombospondin type 1 motif, 9 1, 2
External Ids
HGNC: 132021
Entrez Gene: 569992
UniProtKB: Q9P2N43
Ensembl: ENSG000001636387
Search outside databases for aliases for ADAMTS9 gene

Previous GC identifers: GC03M063400 GC03M063939 GC03M064356 GC03M064458

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ADAMTS9:
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin
motifs) protein family. Members of the family share several distinct protein modules, including a
propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin
type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs,
and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the
cleavage of proteoglycans, the control of organ shape during development, and the inhibition of
angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in
hereditary renal tumors. [provided by RefSeq]

UniProtKB/Swiss-Prot: ATS9_HUMAN, Q9P2N4
Function: Cleaves the large aggregating proteoglycans, aggrecan and versican

Gene Wiki entry for ADAMTS9

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ADAMTS9 gene  

Entrez Gene cytogenetic band: 3p14.3-p14.2   Ensembl cytogenetic band:  3p14.1   HGNC cytogenetic band: 3p14.1

ADAMTS9 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M064476:     (about GC identifiers)

Start:
64,476,370 bp from pter
End:
64,648,405 bp from pter
Size:
172,036 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000003.10  NT_022517.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ATS9_HUMAN, Q9P2N4 (See protein sequence)
Recommended Name: A disintegrin and metalloproteinase with thrombospondin motifs 9 precursor  
Size: 1935 amino acids; 216491 Da
Cofactor: Binds 1 zinc ion per subunit (By similarity)
Subcellular location: Secreted, extracellular space, extracellular matrix (By similarity)
Secondary accessions: A1L4L0 Q9NR29
Alternative splicing: 3 isoforms:  Q9P2N4-3   Q9P2N4-1   Q9P2N4-2   

Post-translational modifications:

  • The precursor is cleaved by a furin endopeptidase (By similarity)1


  • REFSEQ proteins: NP_891550.1  

    ENSEMBL proteins: 
    ENSP00000295903 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (ADAMTS9)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005578 proteinaceous extracellular matrix IDA12514189
    About this table

    Antibodies for ADAMTS9: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (ADAMTS9), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for ADAMTS9

    Assays for ADAMTS9: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/9 InterPro domains/families (see all 9 ):
     IPR000884 Thrombospondin_1_rpt
     IPR018358 Disintegrin_CS
     IPR001818 Pept_M10A_M12B
     IPR010294 ADAM_spacer1
     IPR001590 Peptidase_M12B


       GeneDecks  ADAMTS9 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9P2N4

    ProtoNet protein and cluster: Q9P2N4

    5 Blocks protein families:
    IPB000884 Thrombospondin
    IPB001762 Disintegrin
    IPB002870 Metalloendopeptidase M12B
    IPB008085 Thrombospondin type 1 repeat signature
    IPB010294 ADAM-TS Spacer 1


    UniProtKB/Swiss-Prot: ATS9_HUMAN, Q9P2N4
    Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the
    extracellular matrix (By similarity)
    Domain: The ancillary domains, including the TSRs domain, are required for specific extracellular
    localization and for its versicanase and aggrecanase activities
    Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion,
    thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the
    activation-peptide release activates the enzyme
    Similarity: Contains 1 disintegrin domain
    Similarity: Contains 1 GON domain
    Similarity: Contains 1 peptidase M12B domain
    Similarity: Contains 15 TSP type-1 domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ADAMTS9)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ADAMTS9)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_020249

                  Applied Biosystems Silencer® siRNAs for ADAMTS9

                  Sigma-Aldrich siRNA and siRNA Panels for ADAMTS9  
                         Sigma-Aldrich shRNA Panels and shRNA for ADAMTS9  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_182920
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_182920
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_020249 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_182920

    UniProtKB/Swiss-Prot: ATS9_HUMAN, Q9P2N4
    Function: Cleaves the large aggregating proteoglycans, aggrecan and versican
    Catalytic activity: Cleaves aggrecan at the 1838-Glu- -Ala-1839 site and versican at the 1428-Glu-
    -Ala-1429 site
    Enzyme Number (IUBMB): EC 3.4.24.- 

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004222 metalloendopeptidase activity IEA--
    GO:0008233 peptidase activity IEA--
    GO:0008270 zinc ion binding IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  ADAMTS9 

      --

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508 proteolysis IDA12514189
    GO:0006516 glycoprotein catabolic process TAS10936055
    GO:0007275 multicellular organismal development TAS10936055
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for ADAMTS9
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ADAMTS9

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ADAMTS9)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ADAMTS9)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_020249

                  Sigma-Aldrich siRNA and siRNA Panels for ADAMTS9  
                         Sigma-Aldrich shRNA Panels and shRNA for ADAMTS9  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_182920  

    REFSEQ mRNAs for ADAMTS9 gene: 

    NM_182920.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_182920  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_182920
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_182920
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_020249 

    Additional cDNA sequence: 

    AB037733.1 AF488803.1 AK124945.1 AK300357.1 AK303343.1 AK310571.1 BC026271.2 BC036770.1 
    BC130578.1 BC171764.1 CR619556.1 

    6 DOTS entries:

    DT.100814942  DT.91904315  DT.100684795  DT.40207529  DT.95261455  DT.75158360 

    24/117 AceView cDNA sequences (see all 117 ):

    NM_182921 NM_020249 BE465831 BM729081 N91696 AI217325 AI890599 BM993252 
    AA705567 NM_182920 BF476788 AB037733 BC036770 AI632702 CD686374 CA425143 
    AA382928 CB043880 CA419190 CB215591 AI479925 AW015503 AW338283 AI078611 

    highest scoring ESTs for ADAMTS9:

    AF488803 BC036770 BM923892 AA205581 AA382928 AB037733 AF261918 AK124945 AL597900 AL698299 

    Unigene Cluster for ADAMTS9:

    ADAM metallopeptidase with thrombospondin type 1 motif, 9
    Hs.656071  [show with all ESTs]
    Unigene Representative Sequence: NM_182920


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000295903  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ADAMTS9 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ADAMTS9

    1 / 2 / 3

    8 probe-sets matching ADAMTS9 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    65457_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AW021108 0.40 1.00 0.76 1

    233785_at2, 3 U133-B 1 1.00 1.00 -- -- AB037733 0.60 1.00 0.82 1

    226814_at2, 3 U133-B 1 1.00 1.00 -- -- AI431730 0.40 1.00 0.76 1

    220287_at2, 3 U133-A 1 1.00 1.00 -- -- NM_020249 0.20 1.00 0.72 1

    233785_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    226814_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    220287_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1554697_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  ADAMTS9 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GAAGACACTT

    SOURCE GeneReport for Unigene cluster: Hs.656071

    UniProtKB/Swiss-Prot: ATS9_HUMAN, Q9P2N4
    Tissue specificity: Highly expressed in all fetal tissues. Expressed in a number of adult tissues
    with highest expression in heart, placenta and skeletal muscle

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ADAMTS9 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ADAMTS91   -- ADAM metallopeptidase with thrombospondin type 1 motif, more 90.65(n)
    92.85(a)
    606847  XM_843300.1  XP_848393.1 
    chimpanzee
    (Pan troglodytes)
    ADAMTS91   -- ADAM metallopeptidase with thrombospondin type 1 motif, more 99.45(n)
    99.64(a)
    473333  XM_528704.2  XP_528704.2 
    cow
    (Bos taurus)
    ADAMTS91   -- ADAM metallopeptidase with thrombospondin type 1 motif, more 90.21(n)
    92.06(a)
    537051  XM_617206.3  XP_617206.3 
    rat
    (Rattus norvegicus)
    Adamts91   -- a disintegrin-like and metalloprotease (reprolysin more 86.42(n)
    88.24(a)
    312566  XM_232202.4  XP_232202.4 
    mouse
    (Mus musculus)
    Adamts95 6 a disintegrin-like and metallopeptidase (reprolysin more   -- CN703716 
    About this table        Species with no ortholog for ADAMTS9

    ENSEMBL Gene Tree for ADAMTS9
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ADAMTS9 gene
    ADAMTS152  ADAMTS202  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/1104 NCBI SNPs in ADAMTS9 are shown (see all 1104 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 452)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs43924131,2
    A,C,F,H64649386(+) CGGGAG/AGAGAT 1 -- ng316Minor allele frequency- A:0.18EU EA WA NA 596
    rs67892981,2
    A,C,F,H64648855(+) ACGAAC/TGGGGA 1 -- ng314Minor allele frequency- T:0.07EU EA WA 420
    rs67876331,2
    A,C,F,H64507516(+) GGGCAG/CTCCCT 1 E/D mis112Minor allele frequency- C:0.15NA EU EA WA 1054
    rs10451181,2
    C,F,H64477002(-) CCCAAG/TCAGCT 1 -- ut31 ese35Minor allele frequency- T:0.02MN EU EA WA 598
    rs37963811,2
    C,F,H64502052(-) TCTCCG/CAGGTT 1 Q/E mis1 ese313Minor allele frequency- C:0.01EA EU WA 2004
    rs170709051,2
    C,F,H64482897(+) AAACTC/TGCACC 1 R/Q mis115Minor allele frequency- T:0.03NA EU EA WA 1398
    rs170709671,2
    C,F,H64502315(+) CCTCCT/CTGCAA 1 R/K mis1 ese311Minor allele frequency- C:0.04NA EU EA WA 982
    rs361159501,2
    C,F64647514(+) CTGGGA/TGGAGG 1 S/T mis15Minor allele frequency- T:0.16NA EU EA WA 474
    rs67668011,2
    F,H64476738(+) ATTACT/CGCAGC 1 -- ut314Minor allele frequency- C:0.01EU EA WA 392
    rs44689681,2
    C64649312(+) TCCCCA/TTCTTT 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for ADAMTS9 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605421

    1 Novoseek disease relationship for ADAMTS9 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    necrosis 12.68 2 15880812 (2)
    About this table

    Human Genome Epidemiology Navigator: ADAMTS9 (9 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/26 PubMed articles for ADAMTS9 gene (see all 26 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 56999 HGNC: 13202 AceView: ADAMTS9 Ensembl:ENSG00000163638 euGenes: HUgn56999
    ECgene: ADAMTS9 H-InvDB: ADAMTS9
    (According to HUGE)
    About This Section

    HUGE: KIAA1312
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for ADAMTS9 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ADAMTS9:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for ADAMTS9 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ADAMTS9 Browse small molecules at Sigma-Aldrich
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