ADAMTS8 Gene
protein-coding GIFtS : 54
GC11M129780
ADAM metallopeptidase with thrombospondin type 1 motif, 8 (Previous names: a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8 )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
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Aliases & Descriptions for ADAMTS8
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases ADAM-TS8 1 , 2 , 3 ADAMTS-8 3 EC 3.4.24.- 3 FLJ41712 1 , 2 METH-2 3 METH-8 3 METH2 1 , 2 , 3 , 5
Descriptions ADAM metallopeptidase with thrombospondin type 1 motif, 8 2 ADAM-TS 8 3 a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8 1 , 2
Search outside databases for aliases for ADAMTS8 genePrevious GC identifers: GC11M132275 GC11M131787 GC11M130308 GC11M129812
Summaries for ADAMTS8 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for ADAMTS8 : This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondinmotifs) protein family. Members of the family share several distinct protein modules, including apropeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondintype 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs,and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminalTS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in thevicinity of this gene, most notably lung neoplasms. [provided by RefSeq] UniProtKB/Swiss-Prot: ATS8_HUMAN, Q9UP79 Function : Has anti-angiogenic properties
Gene Wiki entry for ADAMTS8
Genomic Location for ADAMTS8
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the ADAMTS8 gene Entrez Gene cytogenetic band: 11q25 Ensembl cytogenetic band: 11q24.3 HGNC cytogenetic band: 11q25 ADAMTS8 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure
GeneLoc location for GC11M129780:
(about GC identifiers )
Start:
129,780,028 bp from pter
End:
129,803,749 bp from pter
Size:
23,722 bases
Orientation:
minus strand
RefSeq DNA sequence: NC_000011.8 NT_033899.7 Proteins for ADAMTS8
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: ATS8_HUMAN, Q9UP79 (See
protein sequence )Recommended Name: A disintegrin and metalloproteinase with thrombospondin motifs 8 precursor Size : 890 amino acids; 96672 Da
Cofactor : Binds 1 zinc ion per subunit (By similarity)
Subcellular location : Secreted, extracellular space, extracellular matrix (By similarity)
Secondary accessions : Q9NZS0
Post-translational modifications:
The precursor is cleaved by a furin endopeptidase (By similarity)1
REFSEQ proteins: NP_008968.4 ENSEMBL proteins: ENSP00000257359 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 2 Gene Ontology (GO) cellular component terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005576 extracellular region
IEA -- GO:0005578 proteinaceous extracellular matrix
IEA --
About this table Antibodies for ADAMTS8: Assays for ADAMTS8:
Protein
Domains/ Families for ADAMTS8(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q9UP79 ProtoNet protein and cluster: Q9UP79
5 Blocks protein families : IPB000884 Thrombospondin IPB001762 Disintegrin IPB002870 Metalloendopeptidase M12B IPB008085 Thrombospondin type 1 repeat signature IPB010294 ADAM-TS Spacer 1 UniProtKB/Swiss-Prot: ATS8_HUMAN, Q9UP79 Domain : The spacer domain and the TSP type-1 domains are important for a tight interaction with theextracellular matrix Similarity : Contains 1 disintegrin domainSimilarity : Contains 1 peptidase M12B domainSimilarity : Contains 2 TSP type-1 domains
Gene Function for ADAMTS8
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_007037 Applied Biosystems Silencer ® siRNAs for ADAMTS8 Sigma-Aldrich siRNA and siRNA Panels for ADAMTS8 Sigma-Aldrich shRNA Panels and shRNA for ADAMTS8 Explore Sigma-Aldrich super-pooled esiRNAs               OriGene GFP tagged cDNA clone in CMV expression vector: NM_007037                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_007037                                  untagged cDNA clone in CMV expression vector: NM_007037  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_007037 UniProtKB/Swiss-Prot: ATS8_HUMAN, Q9UP79 Function : Has anti-angiogenic propertiesEnzyme Number (IUBMB): EC 3.4.24.-
5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7
):
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Pathways & Interactions for ADAMTS8
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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--2 Gene Ontology (GO) biological process terms (links to tree view) :
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Drugs & Compounds for ADAMTS8 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Enzo Life Sciences drugs & compounds for ADAMTS8
Browse Tocris compounds for ADAMTS8
Transcripts for ADAMTS8(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
Sigma-Aldrich ,
OriGene ,
Tagged/untagged cDNA clones from
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_007037 Sigma-Aldrich siRNA and siRNA Panels for ADAMTS8 Sigma-Aldrich shRNA Panels and shRNA for ADAMTS8 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_007037
REFSEQ mRNAs for ADAMTS8 gene: NM_007037.4
Applied Biosystems TaqMan ® Gene Expression Assays: NM_007037
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_007037                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_007037                                  untagged cDNA clone in CMV expression vector: NM_007037  
Additional cDNA sequence: AB209717.1 AF060153.1 AF175283.1 AK123706.1 BC089435.1 BC113874.1 BC114624.1
8 DOTS entries : DT.120727753 DT.102826821 DT.91753837 DT.101980577 DT.106087 DT.120727736 DT.106086 DT.100845050
24/68 AceView cDNA sequences (see all 68
):CO248849 CA440574 BM988619 AI811557 AA693573 BF221873 CO393893 AI138443 CO398765 CF125500 AA350802 AK123706 CF126031 BM726460 BQ013052 CO246413 AI969885 CO401291 AA917316 BM972024 CO246592 CF125909 AI378857 AI587074
highest scoring ESTs for ADAMTS8 :AF060153 AI400905 AA350802 AA635657 AF175283 AI378857 AI674956 AI969885 AW137467 BF033017
Unigene Cluster for ADAMTS8: ADAM metallopeptidase with thrombospondin type 1 motif, 8 Hs.271605 [show with all ESTs ] Unigene Representative Sequence: AF060153 GeneLoc Exon Structure 4 Alternative Splicing Database (ASD) splice patterns (SP) for ADAMTS8 ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8a · 8b ^ 9 ^ 10 SP1 :             -   -         -       SP2 :         -   -   -   -   -   -   -   -   -     SP3 :                       -       SP4 :                            
About this scheme ECgene alternative splicing isoforms for ADAMTS8 1 Ensembl transcript including schematic representation : ENST00000257359
Expression for ADAMTS8
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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ADAMTS8 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for ADAMTS8 1 / 2 / 3
8 probe-sets matching ADAMTS8 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: TTCTCAGGCTSOURCE GeneReport for Unigene cluster: Hs.271605 UniProtKB/Swiss-Prot: ATS8_HUMAN, Q9UP79 Tissue specificity : Highly expressed in adult and fetal lung, lower expression in brain, placenta,heart, stomach and fetal brain and kidney
Orthologs for ADAMTS8
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for ADAMTS8 gene from 5/6 species (see all 6
)
About this table Species with no ortholog for ADAMTS8 ENSEMBL Gene Tree for ADAMTS8 Paralogs for ADAMTS8 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for ADAMTS8 gene ADAMTS4 2 ADAMTS1 2 ADAMTS5 2
SNPs/Variants for ADAMTS8 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for ADAMTS8 (up to first 250kb)
Disorders & Mutations for ADAMTS8
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 605175
Medical News for ADAMTS8 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for ADAMTS8 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/11 PubMed articles for ADAMTS8 gene (see all 11
): METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity. (PubMed id 10438512) 1, 2, 3, 4 Vazquez F.... Iruela-Arispe M.L. (1999) ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11. (PubMed id 10610729) 1, 3, 4 Georgiadis K.E.... Apte S.S. (1999) METH-2 silencing and promoter hypermethylation in NSCLC. (PubMed id 15328519) 1, 3 Dunn J.R....Liloglou T. (2004) ADAMTS-8 exhibits aggrecanase activity and is expressed in human articular cartilage. (PubMed id 15296936) 1, 3 Collins-Racie L.A....LaVallie E.R. (2004) ADAMTS-4 and -8 are inflammatory regulated enzymes expressed in macrophage-rich areas of human atherosclerotic plaques. (PubMed id 17606262) 3 Wagsater D....Eriksson P. (2008) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross- validation of MS/MS and MS/MS/MS Spectra. (PubMed id 17924679) 4 Yu L.-R.... Veenstra T.D. (2007) ADAMTS8 and ADAMTS15 expression predicts survival in human breast carcinoma. (PubMed id 16152618) 3 Porter S....Edwards D.R. (2006) Expression of ADAMTS-8, a secreted protease with antiangiogenic properties, is downregulated in brain tumours. (PubMed id 16570050) 3 Dunn J.R....Walker C. (2006) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3 Ota T....Sugano S. (2004) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 3 Strausberg R.L....Marra M.A. (2002)
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Genome Databases showing ADAMTS8
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing ADAMTS8
(According to HUGE )
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Specialized Databases showing ADAMTS8 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for ADAMTS8 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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