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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ADAM18 Gene

protein-coding   GIFtS: 47
GCID: GC08P039459

ADAM metallopeptidase domain 18

(Previous name: a disintegrin and metalloproteinase domain 18 )
 Explore 2 diseases affiliated with
ADAM18 via our new
 Human Malady Compendium 
Biological research products
for ADAM18
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
ADAM Metallopeptidase Domain 181 2     ADAM 182 3
ADAM271 2     TMDC III2 3
A Disintegrin And Metalloproteinase Domain 181 2     Disintegrin And Metalloproteinase Domain-Containing Protein 182
TMDCIII1     TMDC33
Transmembrane Metalloproteinase-Like, Disintegrin-Like, And Cysteine-Rich
Protein III2 3
     

External Ids:    HGNC: 1961   Entrez Gene: 87492   Ensembl: ENSG000001686197   UniProtKB: Q9Y3Q73   

Export aliases for ADAM18 gene to outside databases

Previous GC identifers: GC08P038838 GC08P039501 GC08P039182 GC08P038983 GC08P039561 GC08P037975


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ADAM18:
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are
membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of
biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and
neurogenesis. The protein encoded by this gene is a sperm surface protein. Alternatively spliced transcript variants
encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2010)

UniProtKB/Swiss-Prot: ADA18_HUMAN, Q9Y3Q7
Function: Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non
catalytic metalloprotease-like protein (By similarity)

Gene Wiki entry for ADAM18


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000008.10  NC_018919.1  NT_167187.1  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ADAM18 gene promoter:
         CUTL1   Evi-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidADAM18 promoter sequence
   Search SABiosciences Chromatin IP Primers for ADAM18

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ADAM18


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8p11.22   Ensembl cytogenetic band:  8p11.22   HGNC cytogenetic band: 8p11.22

ADAM18 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ADAM18 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P039459:  view genomic region     (about GC identifiers)

Start:
39,442,008 bp from pter      End:
39,587,584 bp from pter
Size:
145,577 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ADA18_HUMAN, Q9Y3Q7 (See protein sequence)
Recommended Name: Disintegrin and metalloproteinase domain-containing protein 18 precursor  
Size: 739 amino acids; 82856 Da
Subcellular location: Membrane; Single-pass type I membrane protein
Secondary accessions: B2R9Y0 Q0VAI4 Q6IRW9 Q6UXJ9
Alternative splicing: 3 isoforms:  Q9Y3Q7-1   Q9Y3Q7-2   Q9Y3Q7-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ADAM18: NX_Q9Y3Q7

Post-translational modifications:

  • The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa (By
  • similarity)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9Y3Q7

  • ADAM18 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001177885.1  NP_055052.1  

    ENSEMBL proteins: 
     ENSP00000429335   ENSP00000265707   ENSP00000369195   ENSP00000429908   ENSP00000428083  
     ENSP00000427730   ENSP00000444729  

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    Uscn Proteins for ADAM18

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005624membrane fraction ----
    GO:0016020membrane TAS10395895
    GO:0016021integral to membrane IEA--


    ADAM18 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ADAM18 for domains           About GeneDecksing

    5/6 InterPro domains/families (see all 6):
     IPR018358 Disintegrin_CS
     IPR024079 MetalloPept_cat_dom
     IPR001762 Blood-coag_inhib_Disintegrin
     IPR006586 ADAM_Cys-rich
     IPR001590 Peptidase_M12B

    Graphical View of Domain Structure for InterPro Entry Q9Y3Q7

    ProtoNet protein and cluster: Q9Y3Q7

    3 Blocks protein families:
    IPB001762 Disintegrin
    IPB002870 Metalloendopeptidase M12B
    IPB006586 ADAM


    UniProtKB/Swiss-Prot: ADA18_HUMAN, Q9Y3Q7
    Domain: A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin
    receptor and thus could mediate sperm/egg binding (By similarity)
    Similarity: Contains 1 disintegrin domain
    Similarity: Contains 1 EGF-like domain
    Similarity: Contains 1 peptidase M12B domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ADA18_HUMAN, Q9Y3Q7
    Function: Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non
    catalytic metalloprotease-like protein (By similarity)

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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004222metalloendopeptidase activity IEA--
    GO:0008237metallopeptidase activity TAS10395895
    GO:0008270zinc ion binding IEA--


    ADAM18 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Adam18tm1Lex for ADAM18
         1 MGI mutant phenotype (inferred from 1 allele(MGI details for Adam18):
     behavior/neurological 

    ADAM18 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ADAM18

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    2 Interacting proteins for ADAM18 (ENSP000002657074) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DLG1ENSP000003457314STRING: ENSP00000345731
    MAD2L1ENSP000002965094STRING: ENSP00000296509
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis IEA--
    GO:0007275multicellular organismal development IEA--
    GO:0007283spermatogenesis IEA--
    GO:0030154cell differentiation IEA--


    ADAM18 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ADAM18
    Search CenterWatch for drugs/clinical trials and news about ADAM18 / ADA18 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ADAM18 gene (2 alternative transcripts): 
    NM_001190956.1  NM_014237.2  

    Unigene Cluster for ADAM18:

    ADAM metallopeptidase domain 18
    Hs.127930  [show with all ESTs]
    Unigene Representative Sequence: NM_014237
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000520001 ENST00000265707(uc003xni.3 uc010lww.3 uc010lwx.3)
    ENST00000379866 ENST00000520772(uc003xnh.3) ENST00000520087 ENST00000522198
    ENST00000520559 ENST00000523755 ENST00000524117 ENST00000541111

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    Inhib. RNA
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    Additional cDNA sequence: 

    AJ133004.1 AK225744.1 AK313961.1 AY358321.1 BC034624.1 BC070279.1 BC121045.1 BC121046.1 

    6 DOTS entries:

    DT.205231  DT.99932611  DT.97839168  DT.91667833  DT.91667835  DT.91667831 

    24/29 AceView cDNA sequences (see all 29):

    BC070279 AY358321 NM_014237 AJ133004 CD385213 BQ429723 BG184589 AI149619 
    BQ432655 BQ435891 BP371488 BG722982 AI073398 BI464271 BE866717 BQ428666 
    AA724715 AA906452 BU182593 AA978174 BC034624 BG201756 AA906424 AA969255 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ADAM18 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AAAGGAATTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ADAM18 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ADAM18

    SOURCE GeneReport for Unigene cluster: Hs.127930

    UniProtKB/Swiss-Prot: ADA18_HUMAN, Q9Y3Q7
    Tissue specificity: Expressed specifically in testis

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    In Situ
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ADAM18

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for ADAM18 gene from 2/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves ADAM26
    Uncharacterized protein
    37(a)
    1 → many
    22(2487128-2497590)
    lizard
    (Anolis carolinensis)
    Reptilia ADAM26
    --
    40(a)
    1 → many
    GL343418.1(598118-621755)


    ENSEMBL Gene Tree for ADAM18 (if available)
    TreeFam Gene Tree for ADAM18 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ADAM18 gene
    ADAM322  ADAM22  ADAMDEC12  
    14 SIMAP similar genes for ADAM18 using alignment to 5 protein entries:     ADA18_HUMAN (see all proteins):
    ADAM32    ADAM22    ADAM9    ADAM2    ADAM21    ADAM30
    ADAM23    ADAM28    ADAM29    ADAM7    ADAM12    ADAM19
    ADAM20    ADAM33

    ADAM18 for paralogs           About GeneDecksing


    4 Pseudogenes.org Pseudogenes for ADAM18
    PGOHUM00000250658 PGOHUM00000263194 PGOHUM00000250025 PGOHUM00000249984


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2522 NCBI SNPs in ADAM18 are shown (see all 2522    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1816611851,2
    --39440123(+) CAATAA/GTAATT 2 -- us2k10--------
    rs1485125461,2
    --39440163(+) CTTAGC/TAGATC 2 -- us2k10--------
    rs1427557671,2
    --39440189(+) ATCAGC/TCTTAT 2 -- us2k10--------
    rs1867921581,2
    C,--39440247(+) GCCCAC/TGCTGG 2 -- us2k10--------
    rs802319321,2
    C,--39440303(+) GTTCAG/AGCAAT 2 -- us2k11Minor allele frequency- A:0.50NA 2
    rs70114921,2
    C,--39440313(+) ttctcC/Ttgcct 2 -- us2k11Minor allele frequency- T:0.50WA 2
    rs1904374481,2
    --39440330(+) TCCAAC/GTACCT 2 -- us2k10--------
    rs1448224541,2
    --39440421(+) GGTCTC/TGAACT 2 -- us2k10--------
    rs1467213181,2
    --39440492(+) CCACCA/GCTCCC 2 -- us2k10--------
    rs1404342551,2
    --39440523(+) TTGCAA/CACAAT 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for ADAM18 (39442008 - 39587584 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 3 variations for ADAM18
         3 CNVs: 3728 2749 7684

    1 SABiosciences Cancer Mutation PCR Assay for ADAM18:
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    141231p.P170S
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ADAM18 for disorders           About GeneDecksing

    2 diseases for ADAM18:    About MalaCards
    epididymitis    melanoma

    Human Genome Epidemiology (HuGE) Navigator: ADAM18 (1 document)

    Export disorders for ADAM18 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ADAM18 gene, integrated from 9 sources (see all 14):
    (articles sorted by number of sources associating them with ADAM18)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human tMDC III: a sperm protein with a potential role in oocyte recognition. (PubMed id 12200459)1, 3, 9 Frayne J....Hall L. (2002)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
    4. Genomewide association study for determinants of HIV-1 acquisition and viral set point in HIV-1 serodiscordant couples with quantified virus exposure. (PubMed id 22174851)1 Lingappa J.R....Goldstein D. (2011)
    5. Analysis of the disintegrin-metalloproteinases family reveals ADAM29 and ADAM7 are often mutated in melanoma. (PubMed id 21618342)2 Wei X.... Samuels Y. (2011)
    6. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    7. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
    8. DNA sequence and analysis of human chromosome 8. (PubMed id 16421571)2 Nusbaum C....Lander E.S. (2006)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 8749 HGNC: 196 AceView: ADAM18 Ensembl:ENSG00000168619 euGenes: HUgn8749
    ECgene: ADAM18 H-InvDB: ADAM18

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ADAM18 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ADAM18 gene:
    Search GeneIP for patents involving ADAM18

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
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