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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ACOT12 Gene

protein-coding   GIFtS: 52
GCID: GC05M080662

acyl-CoA thioesterase 12

 Explore 1 disease affiliated with
ACOT12 via our new
 Human Malady Compendium 
Biological research products
for ACOT12
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Acyl-CoA Thioesterase 121 2 3     START Domain-Containing Protein 152 3
STARD151 2 3     EC 3.1.2.13 8
Cach1 2     Acyl-Coenzyme A Thioesterase 122
THEAL1 2     Cytosolic Acetyl-CoA Hydrolase2
Acyl-CoA Thioester Hydrolase 122 3     StAR-Related Lipid Transfer (START) Domain Containing 152
Cytoplasmic Acetyl-CoA Hydrolase 12 3     CACH3
CACH-12 3     CACH13
HCACH-11     StARD153

External Ids:    HGNC: 244361   Entrez Gene: 1345262   Ensembl: ENSG000001724977   OMIM: 6143155   UniProtKB: Q8WYK03   

Export aliases for ACOT12 gene to outside databases

Previous GC identifer: GC05M075834


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
Function: Hydrolyzes acetyl-CoA to acetate and CoA

Gene Wiki entry for ACOT12


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000005.9  NC_018916.1  NT_006713.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ACOT12 gene promoter:
         Nkx3-1   Nkx3-1 v1   GATA-1   NF-AT   Nkx3-1 v2   AREB6   NF-AT4   NF-AT2   NF-AT3   NF-AT1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidACOT12 promoter sequence
   Search SABiosciences Chromatin IP Primers for ACOT12

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ACOT12


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q14.1   Ensembl cytogenetic band:  5q14.1   HGNC cytogenetic band: 5q14.1

ACOT12 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ACOT12 gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M080662:  view genomic region     (about GC identifiers)

Start:
80,625,824 bp from pter      End:
80,689,998 bp from pter
Size:
64,175 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0 (See protein sequence)
Recommended Name: Acyl-coenzyme A thioesterase 12  
Size: 555 amino acids; 62034 Da
Subunit: Homodimer or homotetramer (By similarity)
Subcellular location: Cytoplasm
1 PDB 3D structure from and Proteopedia for ACOT12:
3B7K (3D)    
Secondary accessions: B3KVK9

Explore the universe of human proteins at neXtProt for ACOT12: NX_Q8WYK0

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8WYK0

  • 3 DME Specific Peptides for ACOT12 (Q8WYK0)
     RHINSAF  ELVLPPHANH  GNTFGGQIMAWME 

    ACOT12 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_570123.1  
    ENSEMBL proteins: 
     ENSP00000303246   ENSP00000421628  

    Human Recombinant Protein Products: 
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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for ACOT12

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829cytosol ISS--


    ACOT12 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ACOT12 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR006683 Thioestr_supf
     IPR023393 START-like_dom
     IPR002913 START_lipid-bd_dom

    Graphical View of Domain Structure for InterPro Entry Q8WYK0

    ProtoNet protein and cluster: Q8WYK0

    UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
    Similarity: Contains 2 acyl coenzyme A hydrolase domains
    Similarity: Contains 1 START domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
    Function: Hydrolyzes acetyl-CoA to acetate and CoA
    Catalytic activity: Acetyl-CoA + H(2)O = CoA + acetate
    Enzyme regulation: Inhibited by ADP. Active in the presence of ATP

    Enzyme Number (IUBMB): EC 3.1.2.11 2

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    1 QIAGEN miScript miRNA Assays for microRNA that regulate ACOT12:
    hsa-miR-217
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    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ACOT12 (see all 7)
    OriGene shRNA RFP: ACOT12
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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003986acetyl-CoA hydrolase activity ISS--
    GO:0004091carboxylesterase activity IEA--
    GO:0005524ATP binding IEA--


    ACOT12 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Pyruvate metabolism
    Pyruvate metabolism1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways



    1         Kegg Pathway  (Kegg details for ACOT12):
        Pyruvate metabolism

    UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
    Pathway: Carbohydrate metabolism; pyruvate metabolism


    ACOT12 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ACOT12

    STRING Interaction Network Preview (showing 5 interactants - click image to see 15)

    5/15 Interacting proteins for ACOT12 (ENSP000003032464) via UniProtKB, MINT, STRING, and/or I2D (see all 15)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    ACSS2ENSP000003538044STRING: ENSP00000353804
    ALDH3A2ENSP000003457744STRING: ENSP00000345774
    ACACAENSP000003447894STRING: ENSP00000344789
    ACACBENSP000003670794STRING: ENSP00000367079
    ACAT1ENSP000002658384STRING: ENSP00000265838
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006084acetyl-CoA metabolic process IEA--
    GO:0006090pyruvate metabolic process IEA--
    GO:0006631fatty acid metabolic process IEA--
    GO:0006637acyl-CoA metabolic process ISS--


    ACOT12 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ACOT12

    4 HMDB Compounds for ACOT12    About this table
    CompoundSynonyms CAS #PubMed Ids
    Acetic acidAcetate (see all 12)64-19-7--
    Acetyl-CoAS-Acetyl coenzyme A (see all 13)72-89-9--
    Coenzyme AAcetoacetyl coenzyme A sodium salt (see all 21)85-61-0--
    WaterDihydrogen oxide (see all 2)7732-18-5--
    Search CenterWatch for drugs/clinical trials and news about ACOT12 / ACO12 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ACOT12 gene: 
    NM_130767.2  

    Unigene Cluster for ACOT12:

    Acyl-CoA thioesterase 12
    Hs.591756  [show with all ESTs]
    Unigene Representative Sequence: AK122960
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000307624(uc003khl.4) ENST00000508234 ENST00000506440 ENST00000513751(uc003khm.3)


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    hsa-miR-217
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    Inhib. RNA
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    Additional cDNA sequence: 

    AB078619.1 AK122960.1 BC075010.2 BC075011.2 BC089437.1 

    5 DOTS entries:

    DT.95151279  DT.213999  DT.101966401  DT.40244066  DT.91976504 

    24/39 AceView cDNA sequences (see all 39):

    BG567019 W93197 BC075010 BC075011 BX094510 AK122960 AA701993 NM_130767 
    AI075132 H38712 AB078619 BX102586 W88442 CF129788 H38009 BG190322 
    H59947 W93105 H38236 W89206 R16116 AV685167 AV693695 H38302 

    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for ACOT12    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b
    SP1:                                                                                                
    SP2:                                                                                                


    ECgene alternative splicing isoforms for ACOT12

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ACOT12 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AAGATACCTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ACOT12 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ACOT12

    SOURCE GeneReport for Unigene cluster: Hs.591756
        SABiosciences Expression via Pathway-Focused PCR Arrays including ACOT12: 
              Fatty Acid Metabolism in human mouse rat
              Molecular Toxicology PathwayFinder 384HT in human mouse rat

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ACOT12

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the last universal common ancestor (LUCA).

    Orthologs for ACOT12 gene from 6/18 species (see all 18)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Acot121 , 5 acyl-CoA thioesterase 121, 5 82.51(n)1
    81.97(a)1
      13 (47.43 cM)5
    741561  NM_028790.31  NP_083066.11 
     917415125 
    chicken
    (Gallus gallus)
    Aves ACOT121 acyl-CoA thioesterase 12 69.86(n)
    63.47(a)
      431627  XM_429179.3  XP_429179.3 
    lizard
    (Anolis carolinensis)
    Reptilia ACOT126
    --
    56(a)
    1 ↔ 1
    2(17489026-17537473)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.98152 Xenopus laevis transcribed sequence with weak similarity more 79.73(n)    BX846471.1 
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1005368731 acyl-coenzyme A thioesterase 12-like 60.87(n)
    54.63(a)
      100536873  XM_003199391.1  XP_003199439.1 
    E. coli
    (Escherichia coli)
    Gamma proteobacteria yciA6
    Acyl-CoA esterase, role unknown
    21(a)
    1 → many
    Chromosome(1309872-1310270)


    ENSEMBL Gene Tree for ACOT12 (if available)
    TreeFam Gene Tree for ACOT12 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ACOT12 gene
    ACOT112  ACOT72  
    1 SIMAP similar gene for ACOT12 using alignment to 2 protein entries:     ACO12_HUMAN (see all proteins):
    ACOT11

    ACOT12 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1241 NCBI SNPs in ACOT12 are shown (see all 1241    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1463503311,2
    --80625492(+) ATACTA/GAATGT 1 -- ds50010--------
    rs744397331,2
    F,--80625573(+) TAATAC/TTACAT 1 -- ds50011Minor allele frequency- T:0.05WA 118
    rs1873038091,2
    --80625634(+) GCCACC/GTGGAA 1 -- ds50010--------
    rs341151481,2
    C,--80625656(+) GACAC-/AAGT  
            
    AAAGT
    1 -- ds50010--------
    rs1919331781,2
    --80625707(+) AATGTC/TTCCCT 1 -- ds50010--------
    rs1430828441,2
    C,--80625889(+) AACCT-/AAAAAA 1 -- ds50010--------
    rs95461,2
    C,F,A,H,--80625946(+) TAGTAC/TTGCAT 1 -- ds5001 ese328Minor allele frequency- T:0.48MN NS EA NA WA CSA 3127
    rs1829086761,2
    --80626060(+) TTGACC/TTAAGA 1 -- ut310--------
    rs1397636951,2
    --80626067(+) AAGATA/GCATTT 1 -- ut310--------
    rs783555181,2
    C,F,--80626085(+) TAACTC/TCGTCA 1 -- ut311Minor allele frequency- T:0.03WA 118

    HapMap Linkage Disequilibrium report for ACOT12 (80625824 - 80689998 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 4 variations for ACOT12
         4 CNVs: 93118 93119 93117 93115

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ACOT12 for disorders           About GeneDecksing

    OMIM gene information: 614315    OMIM disorders: --

    1 disease for ACOT12:    About MalaCards
    pericarditis

    Human Genome Epidemiology (HuGE) Navigator: ACOT12 (1 document)

    Export disorders for ACOT12 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ACOT12 gene, integrated from 9 sources (see all 11):
    (articles sorted by number of sources associating them with ACOT12)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and functional expression of human cytosolic acetyl-CoA hydrolase. (PubMed id 16951743)1, 2, 9 Suematsu N. and Isohashi F. (2006)
    2. A revised nomenclature for mammalian acyl-CoA thioesterases/hydrolases. (PubMed id 16103133)1, 3 Hunt M.C....Alexson S.E. (2005)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Molecular cloning and functional expression of rat liver cytosolic acetyl-CoA hydrolase. (PubMed id 11322891)1, 9 Suematsu N....Isohashi F. (2001)
    6. Genome-wide meta-analyses of nonsyndromic cleft lip wi th or without cleft palate identify six new risk loci. (PubMed id 22863734)1 Ludwig K.U....Nothen M.M. (2012)
    7. Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma. (PubMed id 21248752)2 Varela I....Futreal P.A. (2011)
    8. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    9. Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs. (PubMed id 16940157)3 Hunt M.C....Alexson S.E. (2006)
    10. Simple and unique purification by size-exclusion chromatography for an oligomeric enzyme, rat liver cytosolic acetyl-coenzyme A hydrolase. (PubMed id 12767336)1 Suematsu N....Isohashi F. (2003)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 134526 HGNC: 24436 AceView: CACH-1 Ensembl:ENSG00000172497 euGenes: HUgn134526
    ECgene: ACOT12 Kegg: 134526 H-InvDB: ACOT12

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ACOT12 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ACOT12 gene:
    Search GeneIP for patents involving ACOT12

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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