V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

ACOT12 Gene

protein-coding   GIFtS: 49

GC05M080662
acyl-CoA thioesterase 12
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CACH 3
CACH-1 2, 3
CACH1 3
Cach 1, 2
EC 3.1.2.1 3
MGC105114 2
STARD12 3
STARD15 1, 2
StARD12 3
THEAL 1, 2
hCACH-1 3
Descriptions
Acyl-CoA thioester hydrolase 12 3
Cytoplasmic acetyl-CoA hydrolase 1 3
START domain-containing protein 12 3
StAR-related lipid transfer (START) domain containing 15 2
acyl-CoA thioesterase 12 2, 3
cytosolic acetyl-CoA hydrolase 2
External Ids
HGNC: 244361
Entrez Gene: 1345262
UniProtKB: Q8WYK03
Ensembl: ENSG000001724977
Search outside databases for aliases for ACOT12 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
Function: Hydrolyzes acetyl-CoA to acetate and CoA (By similarity)

Gene Wiki entry for ACOT12

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ACOT12 gene  

Entrez Gene cytogenetic band: 5q14.1   Ensembl cytogenetic band:  5q14.1   HGNC cytogenetic band: 5q14.1

ACOT12 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M080662:     (about GC identifiers)

Start:
80,661,580 bp from pter
End:
80,725,712 bp from pter
Size:
64,133 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_006713.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0 (See protein sequence)
Recommended Name: Acyl-coenzyme A thioesterase 12  
Size: 555 amino acids; 62034 Da
Subunit: Homodimer or homotetramer (By similarity)
Subcellular location: Cytoplasm (By similarity)
PDB structures from and Proteopedia :
3B7K (3D)    

REFSEQ proteins: NP_570123.1  

ENSEMBL proteins: 
ENSP00000303246 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (ACOT12)
Browse Abnova for Human Recombinant Proteins
              OriGene Purified Recombinant Human Protein: ACOT12 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005829 cytosol ISS--
About this table

Antibodies for ACOT12: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (ACOT12), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for ACOT12

Assays for ACOT12: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR006683 Thioestr_supf
 IPR002913 START_lipid_bd


   GeneDecks  ACOT12 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q8WYK0

ProtoNet protein and cluster: Q8WYK0

UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
Similarity: Contains 2 acyl coenzyme A hydrolase domains
Similarity: Contains 1 START domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ACOT12)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_130767

              Applied Biosystems Silencer® siRNAs for ACOT12

              Sigma-Aldrich siRNA for ACOT12  
                     Sigma-Aldrich shRNA for ACOT12  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_130767
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_130767
                                 untagged cDNA clone in CMV expression vector: NM_130767 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_130767

UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
Function: Hydrolyzes acetyl-CoA to acetate and CoA (By similarity)
Catalytic activity: Acetyl-CoA + H(2)O = CoA + acetate
Enzyme Number (IUBMB): EC 3.1.2.1 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003986 acetyl-CoA hydrolase activity ISS--
GO:0004091 carboxylesterase activity IEA--
GO:0005524 ATP binding IEA--
GO:0016787 hydrolase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Sigma-Aldrich "Your Favorite Gene" Pathway for  ACOT12  (Your Favorite Gene powered by Ingenuity) 
 Pyruvate Metabolism

   GeneDecks  ACOT12 for the pathways selected above  
About GeneDecksing

1 Kegg Pathway  (Kegg details for ACOT12):
 hsa00620 Pyruvate metabolism

   GeneDecks  ACOT12 for the pathways selected above  
About GeneDecksing
UniProtKB/Swiss-Prot: ACO12_HUMAN, Q8WYK0
Pathway: Carbohydrate metabolism; pyruvate metabolism


5/17 Interacting proteins for ACOT12 (ENSP000003032463) via UniProtKB, MINT, and/or STRING (see all 17 )

InteractantInteraction Details
GeneCardExternal ID(s)
ACSS2ENSP000003638253STRING (score=.962)
ACAT1ENSP000002658383STRING (score=.939)
ACACAENSP000003447893STRING (score=.899)
ACACBENSP000003410443STRING (score=.899)
ACAT2ENSP000003560153STRING (score=.899)
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006084 acetyl-CoA metabolic process IEA--
GO:0006629 lipid metabolic process IEA--
GO:0006637 acyl-CoA metabolic process ISS--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ACOT12

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ACOT12)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_130767

              Sigma-Aldrich siRNA for ACOT12  
                     Sigma-Aldrich shRNA for ACOT12  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_130767  

REFSEQ mRNAs for ACOT12 gene: 

NM_130767.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_130767  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_130767
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_130767
                                 untagged cDNA clone in CMV expression vector: NM_130767 

Additional cDNA sequence: 

AB078619.1 AK122960.1 BC075010.2 BC075011.2 BC089437.1 

6 DOTS entries:

DT.95151279  DT.213999  DT.101966401  DT.205526  DT.40244066  DT.91976504 

24/39 AceView cDNA sequences (see all 39 ):

BX094510 BC075011 W93197 AK122960 BG567019 BC075010 CF129788 H38009 
AA701993 H38712 AI075132 BG190322 W88442 NM_130767 BX102586 AB078619 
W93105 W89206 H59947 H38236 AV693695 H38302 AV699832 H70112 

highest scoring ESTs for ACOT12:

AB078619 AK122960 AV685167 BC075010 BC075011 BC089437 BX102586 CB162155 CF129788 NM_130767 

Unigene Cluster for ACOT12:

Acyl-CoA thioesterase 12
Hs.591756  [show with all ESTs]
Unigene Representative Sequence: AK122960


GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for ACOT12

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b
SP1:                                                                                                
SP2:                                                                                                

About this scheme

ECgene alternative splicing isoforms for ACOT12

1 Ensembl transcript including schematic representation:
ENST00000307624  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ACOT12 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for ACOT12

1 / 2 / 3

3 probe-sets matching ACOT12 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
58516_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 W89206 0.60 1.00 0.82 1

238160_at2, 3 U133-B 2 1.00 0.95 -- -- R06750 0.40 1.00 0.76 1

238160_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
GeneDecks  ACOT12 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AAGATACCTT

SOURCE GeneReport for Unigene cluster: Hs.591756
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for ACOT12 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
ACOT121   -- acyl-CoA thioesterase 12 88.07(n)
86.08(a)
488925  XM_546042.2  XP_546042.2 
chimpanzee
(Pan troglodytes)
ACOT121   -- acyl-CoA thioesterase 12 99.16(n)
99.1(a)
461769  XM_517670.2  XP_517670.2 
cow
(Bos taurus)
ACOT121   -- acyl-CoA thioesterase 12 88.67(n)
86.62(a)
525155  XM_603502.3  XP_603502.2 
rat
(Rattus norvegicus)
Acot121   -- acyl-CoA thioesterase 12 81.48(n)
79.23(a)
170570  NM_130747.1  NP_570103.1 
mouse
(Mus musculus)
Acot121, 5 135
acyl-CoA thioesterase 121, 5 82.45(n)1
81.97(a)1
741561  NM_028790.21  NP_083066.11 
 AA0665845  AA2650535  (see all 16)
About this table        Species with no ortholog for ACOT12

ENSEMBL Gene Tree for ACOT12
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for ACOT12 gene
ACOT112  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/342 NCBI SNPs in ACOT12 are shown (see all 342 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 105)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 5 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs103711,2
C,F,H,O80667398(+) ATAAGC/TCAAAT 1 A/T mis111Minor allele frequency- T:0.23MN EU EA WA NA 1268
rs95461,2
A,C,F,H80661702(+) TAGTAC/TTGCAT 1 -- ng51 ese311Minor allele frequency- T:0.41MN EU EA WA 1202
rs126977021,2
A,C80725969(+) CTCCCA/CGAGAG 1 -- ng310--------
rs346071741,2
C,F80677545(+) ATCTAC/TGGACT 1 V/I mis15Minor allele frequency- T:0.01NA EU EA WA 490
rs13665971,2
A,F80726000(+) TTTGAG/CATAAA 1 -- ng314Minor allele frequency- C:0.44NA EA 362
rs125171081,2
A,C,F,H80666044(+) agcacG/Cgaaga 1 -- int112Minor allele frequency- C:0.13EU EA WA 1258
rs100780881,2
A,C,F,H80697452(+) aggaaA/Gctaaa 1 -- int1 trp38Minor allele frequency- G:0.48EU EA WA 840
rs77163021,2
A,C,F,H80671375(+) TGAAAC/TGAATT 1 -- int12Minor allele frequency- T:0.08EA 156
rs109422621,2
A,C,F,H80711252(+) TTGACG/AACATT 1 -- int14Minor allele frequency- A:0.38EU EA WA 420
--
rs77335581,2
A,C,F,H80684666(+) agctcT/Cttctt 1 -- int1 trp37Minor allele frequency- C:0.27EU EA WA NA 560
About this table

HapMap Linkage Disequilibrium images for ACOT12 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

8 PubMed articles for ACOT12 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 134526 HGNC: 24436 AceView: CACH-1 Ensembl:ENSG00000172497 euGenes: HUgn134526
ECgene: ACOT12 H-InvDB: ACOT12
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for ACOT12:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for ACOT12-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for ACOT12 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for ACOT12 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Search for Recombinant Proteins
 Search for Antibodies
 Search for Chimera RNAi
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(ACOT12)
 Antibodies (ACOT12)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for ACOT12




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for ACOT12

GeneCards Homepage    -    Last full update: 1 Jul 2009        Incremental update: 13 Oct 2009

Random Gene From GIFtS : about GIFtS

Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science



The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
ACOT12 Gene at Home site.
Version 2.41.1
server4.xennexinc.com