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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ACOT1 Gene

protein-coding   GIFtS: 48
GCID: GC14P074003

acyl-CoA thioesterase 1

 Explore 3 diseases affiliated with
ACOT1 via our new
 Human Malady Compendium 
Biological research products
for ACOT1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Acyl-CoA Thioesterase 11 2 3     Long Chain Acyl-CoA Thioester Hydrolase2 3
ACH21 2     CTE-I2 3
CTE-11 2     CTE-Ib2 3
LACH21 2     EC 3.1.2.23 8
Inducible Cytosolic Acyl-Coenzyme A Thioester Hydrolase2 3     Acyl-Coenzyme A Thioesterase 12
Long Chain Acyl-CoA Hydrolase2 3     CTE13

External Ids:    HGNC: 331281   Entrez Gene: 6413712   Ensembl: ENSG000001842277   OMIM: 6143135   UniProtKB: Q86TX23   

Export aliases for ACOT1 gene to outside databases

Previous GC identifers: GC14U900538 GC14P073074 GC14P054170


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ACOT1_HUMAN, Q86TX2
Function: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty
acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids
and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000014.8  NC_018925.1  NT_026437.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ACOT1 gene promoter:
         AREB6   PPAR-alpha   PPAR-gamma1   HNF-4alpha2   PPAR-gamma2   Arnt   HNF-4alpha1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidACOT1 promoter sequence
   Search SABiosciences Chromatin IP Primers for ACOT1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ACOT1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q24.3   Ensembl cytogenetic band:  14q24.3   HGNC cytogenetic band: 14q24.3

ACOT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ACOT1 gene location

GeneLoc information about chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P074003:  view genomic region     (about GC identifiers)

Start:
74,003,818 bp from pter      End:
74,010,498 bp from pter
Size:
6,681 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ACOT1_HUMAN, Q86TX2 (See protein sequence)
Recommended Name: Acyl-coenzyme A thioesterase 1  
Size: 421 amino acids; 46277 Da
Subunit: Monomer (By similarity)
Subcellular location: Cytoplasm
Secondary accessions: A1L173 Q3I5F9

Explore the universe of human proteins at neXtProt for ACOT1: NX_Q86TX2

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q86TX2

  • 4/10 DME Specific Peptides for ACOT1 (Q86TX2) (see all 10)
     DDHNWKS  WKSEFYA  IEPPYFP  PALGGSF 

    ACOT1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_001032238.1  
    ENSEMBL proteins: 
     ENSP00000311224   ENSP00000451764  

    Human Recombinant Protein Products: 
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    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for ACOT1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005739mitochondrion IDA--
    GO:0005829cytosol IDA16940157


    ACOT1 for ontologies           About GeneDecksing



    ACOT1 Antibody Products: 
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    Uscn ELISAs and CLIAs for ACOT1


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ACOT1 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR006862 Thio_Ohase/aa_AcTrfase
     IPR014940 BAAT_C
     IPR016662 Acyl-CoA_thioEstase_long-chain

    Graphical View of Domain Structure for InterPro Entry Q86TX2

    ProtoNet protein and cluster: Q86TX2

    1 Blocks protein family: IPB006862 Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase

    UniProtKB/Swiss-Prot: ACOT1_HUMAN, Q86TX2
    Similarity: Belongs to the C/M/P thioester hydrolase family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ACOT1_HUMAN, Q86TX2
    Function: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty
    acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids
    and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity)
    Catalytic activity: Palmitoyl-CoA + H(2)O = CoA + palmitate
    Biophysicochemical properties: Kinetic parameters: KM=35.8 uM for C10-acyl-CoA; KM=3.6 uM for C12-acyl-CoA; KM=2.8 uM
    for C14-acyl-CoA; KM=3.6 uM for C16-acyl-CoA; KM=2.4 uM for C18-acyl-CoA; KM=2 uM for C20-acyl-CoA; KM=2.4 uM for
    C16:1-acyl-CoA; KM=4.1 uM for C18:1-acyl-CoA; KM=2.1 uM for C18:1-trans-acyl-CoA; Vmax=224 nmol/min/mg enzyme toward
    C10-acyl-CoA; Vmax=700 nmol/min/mg enzyme toward C12-acyl-CoA; Vmax=912 nmol/min/mg enzyme toward C14-acyl-CoA;
    Vmax=691 nmol/min/mg enzyme toward C16-acyl-CoA; Vmax=597 nmol/min/mg enzyme toward C18-acyl-CoA; Vmax=520 nmol/min/mg
    enzyme toward C20-acyl-CoA; Vmax=577 nmol/min/mg enzyme toward C16:1-acyl-CoA; Vmax=258 nmol/min/mg enzyme toward
    C18:1-acyl-CoA; Vmax=309 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA;

    Enzyme Number (IUBMB): EC 3.1.2.21 2

    miRNA
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    Inhib. RNA
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ACOT1

    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004091carboxylesterase activity IEA--
    GO:0016290palmitoyl-CoA hydrolase activity IEA--
    GO:0047617acyl-CoA hydrolase activity IDA16940157


    ACOT1 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Biosynthesis of unsaturated fatty acids
    Biosynthesis of unsaturated fatty acids1.00
    oleate biosynthesis II (animals)0.32
    2fatty acid alpha-oxidation II
    stearate biosynthesis I (animals)0.43

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    2 BioSystems Pathways for ACOT1 
        stearate biosynthesis I (animals)
    oleate biosynthesis II (animals)


    1         Kegg Pathway  (Kegg details for ACOT1):
        Biosynthesis of unsaturated fatty acids


    ACOT1 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ACOT1

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000038very long-chain fatty acid metabolic process IDA16940157
    GO:0001676long-chain fatty acid metabolic process IDA16940157
    GO:0006629lipid metabolic process ----
    GO:0006637acyl-CoA metabolic process IDA16940157


    ACOT1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ACOT1

    10/49 HMDB Compounds for ACOT1 (see all 49)    About this table
    CompoundSynonyms CAS #PubMed Ids
    (2E)-Decenoyl-CoA(E)-S-2-decenoate Coenzyme A (see all 15)10018-95-8--
    (2E)-Dodecenoyl-CoA2-trans-Dodecenoyl-Coenzyme A (see all 4)1066-12-2--
    (2E)-Hexadecenoyl-CoAtrans-2-Hexadecenoyl-CoA (see all 4)4460-95-1--
    (2E)-Octenoyl-CoA(E)-S-2-octenoate Coenzyme A (see all 16)10018-94-7--
    (2E)-Tetradecenoyl-CoA(2E)-Tetradecenoyl-Coenzyme A (see all 8)38795-33-4--
    11'-Carboxy-alpha-chromanol2,5,7,8-Tetramethyl-2-(4',8'-dimethyl-11'-carboxy-undecyl)-6-chromanol;RRR-11'-carboxy-alpha-chromanol;2-(11-carboxy-4,8-dimethylundecyl)-6-hydroxy-2,5,7,8-tetramethylchroman;11'-carboxy-alpha-tocopherol ----
    11'-Carboxy-alpha-tocotrienolalpha-CDMD(en)(2)HC;2-(10-carboxy-4,8-dimethyl-(3E,7E)-decadienyl)-6-hydroxy-2,5,7,8-tetramethylchroman;alpha-CDMD(en)(,2)HC ----
    11'-Carboxy-gama-chromanol2,7,8-trimethyl-2-(11'-carboxy-4',8'-dimethylundecyl)-6-chromanol;2,7,8-trimethyl-2-(11'-carboxy-4',8'-dimethylundecyl)-6-hydroxychroman;RRR-11'-carboxy-gama-chromanol;11'-Carboxy-gama-tocopherol ----
    11'-carboxy-gama-tocotrienolgama-CDMD(en)(2)HC;carboxydimethyldecadienyl hydroxychroman;2-(10-carboxy-4,8-dimethyl-(3E,7E)-decadienyl)-6-hydroxy-2,7,8-trimethylchroman ----
    13'-carboxy-alpha-tocotrienol13AaTE ----
    Search CenterWatch for drugs/clinical trials and news about ACOT1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ACOT1 gene: 
    NM_001037161.1  

    Unigene Cluster for ACOT1:

    Acyl-CoA thioesterase 1
    Hs.568046  [show with all ESTs]
    Unigene Representative Sequence: BX161396
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000311148(uc001xol.1 uc010tuc.2) ENST00000557556

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    Additional cDNA sequence: 

    AK300886.1 BC127747.1 BC127748.1 BC132889.1 BC132891.1 BC143042.1 BX161396.1 DQ082754.1 
    GU727640.1 

    9 DOTS entries:

    DT.100824510  DT.445656  DT.95157623  DT.100824506  DT.91760359  DT.97813369  DT.100824503  DT.100824511 
    DT.86853383 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ACOT1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ACOT1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ACOT1

    SOURCE GeneReport for Unigene cluster: Hs.568046
        SABiosciences Expression via Pathway-Focused PCR Arrays including ACOT1: 
              Fatty Acid Metabolism in human mouse rat
              Molecular Toxicology PathwayFinder 384HT in human mouse rat

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ACOT1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for ACOT1 gene from 4/10 species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves LOC7717531 acyl-coenzyme A thioesterase 2, mitochondrial-like 69.99(n)
    67.7(a)
      771753  XM_003641392.1  XP_003641440.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    61(a)
    possible ortholog
    1(39211695-39218982)
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkey-11e23.46
    si:dkey-288i20.16
    (see all 9)
    si:dkey-288i20.1
    (see all 9)
    44(a)
    43(a)
    (see all 9)
    many ↔ many
    many ↔ many
    (see all 9)
    20(28961321-28964638)
    16(3418191-3422128)
    worm
    (Caenorhabditis elegans)
    Secernentea K05B2.46
    W03D8.86
    (see all 4)
    Protein W03D8.8
    (see all 4)
    27(a)
    26(a)
    (see all 4)
    many ↔ many
    many ↔ many
    (see all 4)
    X(4918989-4920948)
    I(2791691-2794897)


    ENSEMBL Gene Tree for ACOT1 (if available)
    TreeFam Gene Tree for ACOT1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ACOT1 gene
    ACOT62  ACOT42  ACOT22  BAAT2  
    4 SIMAP similar genes for ACOT1 using alignment to 3 protein entries:     ACOT1_HUMAN (see all proteins):
    ACOT2    ACOT4    ACOT6    BAAT

    ACOT1 for paralogs           About GeneDecksing


    2 Pseudogenes.org Pseudogenes for ACOT1
    PGOHUM00000258798 PGOHUM00000256743


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 24 variations for ACOT1
         15/24 CNVs (see all 24): 71917 76672 37822 35265 7039 71916 87267 3942 35269 101980 87266 58686 31974 35267 30658
    1 SABiosciences Cancer Mutation PCR Assay for ACOT1:
    Cosmic IdAA Change
    98000001
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ACOT1
    DNA2.0 Custom Variant and Variant Library Synthesis for ACOT1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ACOT1 for disorders           About GeneDecksing

    OMIM gene information: 614313    OMIM disorders: --

    3 diseases for ACOT1:    About MalaCards
    human immunodeficiency virus infectious disease    epididymitis    immunodeficiency

    1 disease from the University of Copenhagen DISEASES database for ACOT1:
    Human immunodeficiency virus infectious disease

    Export disorders for ACOT1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ACOT1 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with ACOT1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs. (PubMed id 16940157)1, 2, 3 Hunt M.C....Alexson S.E. (2006)
    2. A revised nomenclature for mammalian acyl-CoA thioesterases/hydrolases. (PubMed id 16103133)1, 3 Hunt M.C....Alexson S.E. (2005)
    3. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    4. Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins. (PubMed id 20736409)1 Li J.... Liu Y. (2010)
    5. Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    8. Identification of PTE2, a human peroxisomal long-chain acyl-CoA thioesterase. (PubMed id 10944470)9 Jones J.M. and Gould S.J. (2000)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 641371 HGNC: 33128 Ensembl:ENSG00000184227 euGenes: HUgn641371 ECgene: ACOT1 Kegg: 641371
    H-InvDB: ACOT1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ACOT1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ACOT1 gene:
    Search GeneIP for patents involving ACOT1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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