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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

ACLY Gene

protein-coding GIFtS: 64
GCID: GC17M040023

ATP citrate lyase

Explore 15 diseases affiliated with
ACLY via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
ATP Citrate Lyase¹ ²		ATP-Citrate (Pro-S-)-Lyase² ³
ACL¹ ² ³		EC 2.3.3.8³ ⁸
ATPCL¹ ²		ATP Citrate Synthase²
CLATP¹ ²		ATP-Citrate Synthase²
Citrate Cleavage Enzyme² ³

External Ids:	HGNC: 115¹	Entrez Gene: 47²	Ensembl: ENSG00000131473⁷	OMIM: 108728⁵	UniProtKB: P53396³

Export aliases for ACLY gene to outside databases

Previous GC identifers: GC17M039579 GC17M042174 GC17M039931 GC17M040396 GC17M037276 GC17M035785

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ACLY:

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The

enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes

the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and

phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and

cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two

transcript variants encoding distinct isoforms have been identified for this gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: ACLY_HUMAN, P53396

Function: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many

tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of

acetylcholine

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000017.10 NC_018928.1 NT_010783.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the ACLY gene promoter:
Max1 c-Myc C/EBPalpha
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): ACLY promoter sequence

Search SABiosciences Chromatin IP Primers for ACLY

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ACLY

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q21.2 Ensembl cytogenetic band: 17q21.2 HGNC cytogenetic band: 17q21.2

ACLY Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ACLY gene location

GeneLoc information about chromosome 17 GeneLoc Exon Structure

GeneLoc location for GC17M040023: view genomic region (about GC identifiers)

Start:	40,023,161 bp from pter	End:	40,086,795 bp from pter
Size:	63,635 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ACLY_HUMAN, P53396 (See protein sequence)

Recommended Name: ATP-citrate synthase

Size: 1101 amino acids; 120839 Da

Subunit: Homotetramer

Subcellular location: Cytoplasm

3 PDB 3D structures from and Proteopedia for ACLY:

3MWD (3D)

3MWE (3D)

3PFF (3D)

Secondary accessions: B4DIM0 Q13037 Q9BRL0

Alternative splicing: 2 isoforms: P53396-1 P53396-2

Explore the universe of human proteins at neXtProt for ACLY: NX_P53396

Post-translational modifications:

ISGylated¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P53396

4/30 DME Specific Peptides for ACLY (P53396) (see all 30)

KQGLYRH

WCIGTCA

FTYLEINP

TTDGVYEG

ACLY Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001087.2 NP_942127.1

ENSEMBL proteins:

ENSP00000253792 ENSP00000466259 ENSP00000445349 ENSP00000377474 ENSP00000464717

ENSP00000468705 ENSP00000345398

Reactome Protein details: P53396
Human Recombinant Protein Products for ACLY:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Novus Biologicals ACLY Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for ACLY

Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	--
GO:0005739	mitochondrion	IEA	--
GO:0005829	cytosol	TAS	--

ACLY for ontologies About GeneDecksing

ACLY Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	Novus Biologicals ACLY Antibodies
	Abcam antibodies for ACLY
	Uscn Antibodies for ACLY
	Search ThermoFisher Antibodies for ACLY

Assay Products for ACLY:

	Browse Kits and Assays available from EMD Millipore
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	GenScript Custom Assay Services for ACLY
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for ACLY

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ACLY for domains About GeneDecksing

5/13 InterPro domains/families (see all 13):

IPR017866 Succ-CoA_synthase_bsu_CS

IPR013650 ATP-grasp_succ-CoA_synth-type

IPR016143 Citrate_synth-like_sm_a-sub

IPR014608 ATP-citrate_synthase

IPR005810 CoA_lig_alpha

Graphical View of Domain Structure for InterPro Entry P53396

ProtoNet protein and cluster: P53396

2 Blocks protein families:

IPB003781 CoA Binding Domain

IPB005809 Succinyl-CoA synthetase

UniProtKB/Swiss-Prot: ACLY_HUMAN, P53396

Similarity: In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family

Similarity: In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: ACLY_HUMAN, P53396

Function: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many

tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of

acetylcholine

Catalytic activity: ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA

Genatlas biochemistry entry for ACLY:

ATP-citrate lyase,catalyzing the synthesis of the cytosolic acetyl-CoA,used as a substrate for de novo biosynthesis of

fatty acid and cholesterol

Enzyme Number (IUBMB): EC 2.3.3.8¹ ²

Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000166	nucleotide binding	--	--
GO:0003878	ATP citrate synthase activity	TAS	--
GO:0004775	succinate-CoA ligase (ADP-forming) activity	IEA	--
GO:0005524	ATP binding	IEA	--
GO:0046872	metal ion binding	IEA	--

ACLY for ontologies About GeneDecksing

Phenotypes:
2 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Acly):

homeostasis/metabolism

mortality/aging

ACLY for phenotypes About GeneDecksing

Animal Models:

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for ACLY

miRNA Products:		OriGene 3'-UTR Clone (see all 2): ACLY Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ACLY
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate ACLY (see all 14):
		hsa-miR-194* hsa-miR-22 hsa-miR-513a-5p hsa-miR-27a hsa-miR-3120-3p hsa-miR-374c hsa-miR-214 hsa-miR-567

		SwitchGear 3'UTR luciferase reporter plasmid: ACLY 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ACLY (see all 7) OriGene shRNA RFP: ACLY OriGene siRNA: ACLY
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		Sirion Biotech Custom design and validation of potent shRNA sequences against ACLY

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for ACLY
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Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ACLY (see all 5) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ACLY (see all 2) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): ACLY (NM_198830)
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		Sirion Biotech Customized stable knockdown cell line services for ACLY Sirion Biotech Customized inducible knockdown cell line services for ACLY Sirion Biotech Customized stable overexpressing cell line services for ACLY Sirion Biotech Customized inducible overexpressing cell line services for ACLY

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ACLY

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/10 super-pathways (see all 10) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Metabolism

	Metabolism	1.00			Metabolism of lipids and lipoproteins	0.34
	Metabolic pathways	0.38

Saturated fatty acid biosynthesis

	ChREBP activates metabolic gene expression	0.38			acetyl-CoA biosynthesis III (from citrate)	0.00
	Transcriptional activation of Citrate lyase monomer gene by ChREBP:MLX	0.00

Fatty Acyl-CoA Biosynthesis

Fatty Acyl-CoA Biosynthesis

1.00

Triglyceride Biosynthesis

0.47

Development_CNTF receptor signaling

Regulation of lipid metabolism_Insulin signaling-generic cascades

0.35

Regulation of lipid metabolism Insulin signaling:generic cascades

0.35

Glucose / Energy Metabolism

Glucose / Energy Metabolism

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

1 EMD Millipore Pathway for ACLY

Regulation of lipid metabolism Insulin signaling-generic cascades

1 Cell Signaling Technology (CST) Pathway for ACLY

Glucose / Energy Metabolism

1 GeneGo (Thomson Reuters) Pathway for ACLY

Regulation of lipid metabolism Insulin signaling-generic cascades

3 BioSystems Pathways for ACLY

	SREBP signalling
	Fatty Acid Biosynthesis
	acetyl-CoA biosynthesis III (from citrate)

5/8 Reactome Pathways for ACLY (see all 8)

	Metabolism
	Triglyceride Biosynthesis
	Integration of energy metabolism
	Fatty acid, triacylglycerol, and ketone body metabolism
	ChREBP activates metabolic gene expression

2 Kegg Pathways (Kegg details for ACLY):

	Citrate cycle (TCA cycle)
	Metabolic pathways

ACLY for pathways About GeneDecksing

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for ACLY

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/102 Interacting proteins for ACLY (P53396^{2, 3} ENSP00000253792⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 102)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
NME2	P22392³, ENSP00000365572⁴	I2D: score=2 STRING: ENSP00000365572
CDKN2A	P42771³, ENSP00000355153⁴	I2D: score=1 I2D: score=1 STRING: ENSP00000355153
PSMB1	P20618³, ENSP00000262193⁴	I2D: score=5 STRING: ENSP00000262193
GSK3B	P49841³, ENSP00000324806⁴	I2D: score=4 STRING: ENSP00000324806
NME1-NME2	P22392³	I2D: score=2

About this table

Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006101	citrate metabolic process	TAS	1371749
GO:0006112	energy reserve metabolic process	TAS	--
GO:0006200	ATP catabolic process	TAS	1371749
GO:0015936	coenzyme A metabolic process	TAS	1371749
GO:0019432	triglyceride biosynthetic process	TAS	--

ACLY for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

ACLY for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Enzo Life Sciences drugs & compounds for ACLY

Browse Tocris compounds for ACLY

7 HMDB Compounds for ACLY About this table

Compound	Synonyms	CAS #	PubMed Ids
ADP	adenosindiphosphorsaeure (see all 8)	58-64-0	--
Acetyl-CoA	S-Acetyl coenzyme A (see all 13)	72-89-9	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Citric acid	2-Hydroxy-1,2,3-propanetricarboxylate (see all 20)	77-92-9	--
Coenzyme A	Acetoacetyl coenzyme A sodium salt (see all 21)	85-61-0	--
Oxalacetic acid	2-Ketosuccinate (see all 20)	328-42-7	--
Phosphate	NFB Orthophosphate (see all 13)	14265-44-2	--

10/28 Novoseek chemical compound relationships for ACLY gene (see all 28) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
hydroxycitrate	91	4	17476502 (1), 11319829 (1), 20372858 (1)
(-)-hydroxycitrate	89.1	3	2176080 (1), 11101469 (1)
acetyl-coa	84	26	14681844 (2), 8207683 (1), 7911658 (1), 11171136 (1) (see all 21)
citrate	77.7	31	17928289 (2), 1765100 (2), 8207683 (1), 9820262 (1) (see all 21)
oxaloacetate	69.9	7	7669753 (2), 11171137 (1), 1675605 (1), 18922930 (1)
phosphohistidine	69.2	2	1371749 (1)
pyruvate	62.2	14	8999918 (3), 17928289 (2), 11171136 (1), 7616129 (1) (see all 10)
fatty acid	62.1	26	10410463 (3), 8999918 (3), 15869874 (1), 17476502 (1) (see all 15)
6-phosphogluconate	60.9	2	14605988 (1), 8355562 (1)
3-hydroxy-3-methylglutaryl-coa	57.8	7	8999918 (2), 18774944 (1), 19389950 (1)

Search CenterWatch for drugs/clinical trials and news about ACLY

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for ACLY gene (2 alternative transcripts):

NM_001096.2 NM_198830.1

Unigene Cluster for ACLY:

ATP citrate lyase

Hs.387567 [show with all ESTs]

Unigene Representative Sequence: NM_001096

10 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000352035(uc002hyg.3 uc002hyi.3) ENST00000590151 ENST00000537919

ENST00000393896 ENST00000588779 ENST00000590770 ENST00000588547 ENST00000590735

ENST00000592970 ENST00000353196(uc002hyh.3 uc010wfx.2 uc010wfy.2)

miRNA Products:		OriGene 3'-UTR Clone (see all 2): ACLY Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ACLY
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate ACLY (see all 14):
		hsa-miR-194* hsa-miR-22 hsa-miR-513a-5p hsa-miR-27a hsa-miR-3120-3p hsa-miR-374c hsa-miR-214 hsa-miR-567

		SwitchGear 3'UTR luciferase reporter plasmid: ACLY 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
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		Sirion Biotech Custom design and validation of potent shRNA sequences against ACLY

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		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ACLY
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ACLY

Additional cDNA sequence:

AB210035.1 AK058079.1 AK095084.1 AK295675.1 AK304802.1 AK312315.1 BC006195.2 U18197.1

X64330.1

22 DOTS entries:

DT.40188466 DT.100820527 DT.100665906 DT.100820535 DT.91760382 DT.120917271 DT.100642777 DT.120917233

DT.95161296 DT.86850803 DT.95211622 DT.101977534 DT.120917265 DT.120917268 DT.91652223 DT.91760383

DT.91808785 DT.95161274 DT.95161295 DT.95359867 DT.97862140 DT.91697249

24/523 AceView cDNA sequences (see all 523):

BP364222 AI697052 AI632007 AI573048 AA363991 AW872778 BM549171 AI906366

BP356928 BQ774639 AI364214 NM_198830 BQ024361 NM_001096 BQ431218 BQ059802

BQ430304 AI885349 BQ678057 BU160670 BP363686 AU130024 AW672850 AI497943

GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for ACLY (see all 9) About this scheme

ExUns:	1a	·	1b	·	1c	·	1d	^	2a	·	2b	^	3	^	4	^	5	^	6	^	7	^	8a	·	8b	^	9	^	10a	·	10b	^	11	^	12	^	13	^	14	^	15a	·	15b	^	16a	·	16b	^	17a	·	17b	^
SP1:																																									-										-
SP2:																																									-										-
SP3:																																							-		-										-
SP4:									-		-		-
SP5:																																																			-

ExUns:	18	^	19a	·	19b	^	20	^	21	^	22	^	23	^	24	^	25a	·	25b	^	26	^	27a	·	27b	^	28	^	29	^	30a	·	30b	·	30c
SP1:																					-
SP2:																					-
SP3:																					-
SP4:
SP5:

ECgene alternative splicing isoforms for ACLY

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

ACLY expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AAGAACATTG

About this image

ACLY expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

5 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Liver

Liver Lobule

Periportal Hepatocytes

Selective marker (cell-identifying gene)

Liver

Neural Tube

Spinal Neural Tube Cells

Neural Ectoderm

Eye

Retina

Eye

Heart

Atrioventricular Canal

Heart

Spinal Cord

Presumptive Spinal Cord

Spinal Cord

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See ACLY Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ACLY

SOURCE GeneReport for Unigene cluster: Hs.387567

SABiosciences Expression via Pathway-Focused PCR Arrays including ACLY:

Cancer PathwayFinder in human mouse rat

Fatty Liver in human mouse rat

Diabetes in human mouse rat

Molecular Toxicology PathwayFinder 384HT in human mouse rat

Glucose Metabolism in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ACLY Browse OriGene validated miRNA SYBR primer pairs
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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ACLY

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ACLY

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for ACLY gene from 9/34 species (see all 34) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Acly^{1 , 5}	ATP citrate lyase^{1, 5}	90.25(n)¹ 98.09(a)¹		11 (63.47 cM)⁵ 104112¹ NM_001199296.1¹ NP_001186225.1¹ 100476353⁵
chicken (Gallus gallus)	Aves	ACLY¹	ATP citrate lyase	80.23(n) 93.19(a)		395373 NM_001030540.1 NP_001025711.1
lizard (Anolis carolinensis)	Reptilia	ACLY⁶	--	92(a)	1 ↔ 1	6(66511453-66551297)
African clawed frog (Xenopus laevis)	Amphibia	Xl.11981²	Xenopus laevis transcribed sequence with strong similarity to protein pirS21173 (H.sapiens) S21173 ATP citrate (pro-S)-lyase - human less	79.63(n)		BX846323.1
zebrafish (Danio rerio)	Actinopterygii	aclya¹	ATP citrate lyase a	76.75(n) 87.17(a)		436922 NM_001002649.1 NP_001002649.1
fruit fly (Drosophila melanogaster)	Insecta	ATPCL^{1 , 3}	acetyl-CoA biosynthesis ATP-citrate (pro-S)-lyase less³ ATP citrate lyase¹	67(a)³ 66.39(n)¹ 70.46(a)¹		36760¹ NM_166131.2¹ NP_725514.2¹
worm (Caenorhabditis elegans)	Secernentea	B0365.1³ acly-2¹	ATP citrate lyase³ Protein ACLY-2¹	59(a) (best of 2)³ 58.63(n)¹ 62.52(a)¹		V(13119107-13122639)³ 179795¹ NM_073866.2¹ NP_506267.1¹
thale cress (Arabidopsis thaliana)	eudicotyledons	ACLB-1¹	ATP-citrate lyase B-1	56.91(n) 53.58(a)		819845 NM_111541.3 NP_187317.1
rice (Oryza sativa)	Liliopsida	Os.8730²	Oryza sativa (japonica cultivar-group) cDNA cloneJ013055I05, full insert sequence less	74.74(n)		AK062011.1

ENSEMBL Gene Tree for ACLY (if available)
TreeFam Gene Tree for ACLY (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
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Paralogs for ACLY gene

SUCLG1²

1 SIMAP similar gene for ACLY using alignment to 4 protein entries: ACLY_HUMAN (see all proteins):

ACLY variant protein

ACLY for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 17 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs111597057^1,2	C	--	35784891(+)	`AGCAGG/AACCTT`	1	--	us2k¹		1		CSA	1
rs111326058^1,2	C	--	35784945(+)	`CTCCTG/TTAGTC`	1	--	us2k¹		1		WA	2
rs112685370^1,2		--	35785083(+)	`TACAGC/TTACAA`	1	--	us2k¹		1		CSA	2
rs1058928^1,2	F	--	35785219(-)	`AATCCA/CCAAAG`	1	--	us2k¹ ese³		1		MN	184
rs71228789^1,2	C	--	35785580(-)	`CTCTCT/-TTTTT`	1	--	us2k¹		1		NA	2
rs9944422^1,2	C,F	--	40022724(+)	`GCCTCG/AGCCTC`	2	--	ds500¹		4		NA WA CSA	7
rs186811471^1,2		--	40022761(+)	`GCCACC/TACGCC`	2	--	ds500¹		0	--	--	--	--
rs9303319^1,2	C,F,A,H	--	40022809(+)	`AAACTG/ATTAGT`	2	--	ds500¹		22		NS EA WA NA CSA	2346
rs199763985^1,2		--	40022908(+)	`CCAGA-/CCAGAGC`	2	--	ds500¹		0	--	--	--	--
rs9303320^1,2	C	--	40022909(+)	`CCAGAC/AGAGCC`	2	--	ds500¹		4		NA WA EA	360

HapMap Linkage Disequilibrium report for ACLY (40023161 - 40086795 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for ACLY: --
Human Gene Mutation Database (HGMD): ACLY

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ACLY

DNA2.0 Custom Variant and Variant Library Synthesis for ACLY

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

ACLY for disorders           About GeneDecksing

OMIM gene information: 108728    OMIM disorders: --

15 diseases for ACLY:    About MalaCards

type 1 diabetes mellitus diabetes mellitus hepatocellular carcinoma lung cancer

prostate cancer glioblastoma obesity cholesterol

prostatitis schizophrenia thyroiditis carcinoma

pancreatitis hepatitis neuronitis

2 Novoseek disease relationships for ACLY gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
tumors	0	3	16226706 (1), 18922930 (1), 18796435 (1)
cancer	0	3	16778563 (2), 20372858 (1)

Human Genome Epidemiology (HuGE) Navigator: ACLY (6 documents)

Export disorders for ACLY gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for ACLY gene, integrated from 9 sources (see all 146):
(articles sorted by number of sources associating them with ACLY)

Utopia: connect your pdf to the dynamic
world of online information

Cloning and expression of a human ATP-citrate lyase cDNA. (PubMed id 1371749)^{1, 2, 3, 9} Elshourbagy N.A.... Gloger I.S. (1992)
Localization of the gene for ATP citrate lyase (ACLY) distal to gastrin(GAS) and proximal to D17S856 on chromosome 17q12-q21. (PubMed id 8088842)^{1, 3, 9} Couch F.J....Weber B.L. (1994)
Identification of the citrate-binding site of human A TP-citrate lyase using X-ray crystallography. (PubMed id 20558738)^{1, 2} Sun T....Fraser M.E. (2010)
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{1, 2} Olsen J.V....Mann M. (2006)
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (PubMed id 15592455)^{1, 2} Rush J.... Comb M.J. (2005)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Variant cDNA sequences of human ATP:citrate lyase: cloning, expression, and purification from baculovirus-infected insect cells. (PubMed id 9116495)^{1, 2} Lord K.A....Ciccarelli R.B. (1997)
ATP citrate lyase: activation and therapeutic implications in non-small cell lung cancer. (PubMed id 18922930)^{1, 9} Migita T....Ishikawa Y. (2008)
Sequence of sites on ATP-citrate lyase and phosphatase inhibitor 2 phosphorylated by multifunctional protein kinase (a glycogen synthase kinase 3 like kinase). (PubMed id 2176822)^{1, 9} Ramakrishna S....Benjamin W.B. (1990)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 47	HGNC: 115	AceView: ACLY	Ensembl:ENSG00000131473	euGenes: HUgn47
ECgene: ACLY	Kegg: 47	H-InvDB: ACLY

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for ACLY	Pharmacogenomics, SNPs, Pathways

Intellectual Property for ACLY gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for ACLY gene:
Search GeneIP for patents involving ACLY

GeneCards and IP:
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ATP citrate lyase

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ATP citrate lyase

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