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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

ACACB Gene

protein-coding GIFtS: 65
GCID: GC12P109577

acetyl-CoA carboxylase beta

(Previous name: acetyl-Coenzyme A carboxylase beta )

Explore 17 diseases affiliated with
ACACB via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Acetyl-CoA Carboxylase Beta¹ ²		Acetyl-Coenzyme A Carboxylase Beta¹ ²
ACC2¹ ² ³ ⁵		ACC-Beta¹
ACCB¹ ² ³ ⁵		Acetyl-CoA Carboxylase 2²
HACC275¹ ²		EC 6.4.1.2³

External Ids:	HGNC: 85¹	Entrez Gene: 32²	Ensembl: ENSG00000076555⁷	OMIM: 601557⁵	UniProtKB: O00763³

Export aliases for ACACB gene to outside databases

Previous GC identifers: GC12P108635 GC12P109416 GC12P108039 GC12P108017 GC12P108062 GC12P106595

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ACACB:

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which

catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is

thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA

transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in

the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two

ACC-beta isoforms. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: ACACB_HUMAN, O00763

Function: ACC-beta may be involved in the provision of malonyl-CoA or in the regulation of fatty acid oxidation, rather

than fatty acid biosynthesis. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and

carboxyltransferase

Gene Wiki entry for ACACB

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000012.11 NC_018923.1 NT_009775.17

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the ACACB gene promoter:
c-Fos STAT1 RFX1 MAZR AP-1 STAT1beta C/EBPalpha STAT1alpha c-Jun
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): ACACB promoter sequence

Search SABiosciences Chromatin IP Primers for ACACB

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ACACB

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q24.11 Ensembl cytogenetic band: 12q24.11 HGNC cytogenetic band: 12q24.1

ACACB Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ACACB gene location

GeneLoc information about chromosome 12 GeneLoc Exon Structure

GeneLoc location for GC12P109577: view genomic region (about GC identifiers)

Start:	109,554,400 bp from pter	End:	109,706,031 bp from pter
Size:	151,632 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ACACB_HUMAN, O00763 (See protein sequence)

Recommended Name: Acetyl-CoA carboxylase 2

Size: 2458 amino acids; 276541 Da

Cofactor: Biotin (By similarity)

Cofactor: Binds 2 manganese ions per subunit (By similarity)

Subunit: Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts with MID1IP1; interaction with

MID1IP1 promotes oligomerization and increases its activity

Subcellular location: Endomembrane system. Note=May associate with membranes

Sequence caution: Sequence=AAB58382.1; Type=Miscellaneous discrepancy; Note=Many Frameshifts and conflicts;

Sequence=CAE01470.2; Type=Erroneous translation; Note=Wrong choice of CDS;

6/9 PDB 3D structures from and Proteopedia for ACACB (see all 9):

2DN8 (3D)

2HJW (3D)

2KCC (3D)

3FF6 (3D)

3GID (3D)

3GLK (3D)

Secondary accessions: A6NK36 Q16852 Q1HEC1 Q6KE87 Q6KE89 Q6TY48

Alternative splicing: 2 isoforms: O00763-1 O00763-2

Explore the universe of human proteins at neXtProt for ACACB: NX_O00763

Post-translational modifications:

Phosphorylated by AMPK, leading to inactivate the enzyme¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_O00763

ACACB Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_001084.3

ENSEMBL proteins:

ENSP00000341044 ENSP00000443494 ENSP00000443281 ENSP00000443625 ENSP00000379534

ENSP00000445172 ENSP00000367079 ENSP00000367085 ENSP00000444075

Reactome Protein details: O00763
Human Recombinant Protein Products:

	EMD Millipore Purified and/or Recombinant ACACB Protein
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom Protein Services for ACACB
	GenScript Custom Purified and Recombinant Proteins Services for ACACB
	Novus Biologicals ACACB Protein
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Browse Proteins at Uscn

Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	IDA	--
GO:0005739	mitochondrion	IEA	--
GO:0005794	Golgi apparatus	--	--
GO:0005829	cytosol	TAS	--
GO:0012505	endomembrane system	IEA	--

ACACB for ontologies About GeneDecksing

ACACB Antibody Products:

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	Novus Biologicals ACACB Antibodies
	Abcam antibodies for ACACB
	Browse Antibodies at Uscn
	Search ThermoFisher Antibodies for ACACB

Assay Products for ACACB:

	Browse Kits and Assays available from EMD Millipore
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	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for ACACB
	Cell Signaling Technology (CST) Sandwich ELISA Kits for ACACB (ACC2)
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs and CLIAs at Uscn

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ACACB for domains About GeneDecksing

5/15 InterPro domains/families (see all 15):

IPR011762 COA_CT_N

IPR011053 Single_hybrid_motif

IPR000022 Carboxyl_trans

IPR005479 CbamoylP_synth_lsu-like_ATP-bd

IPR000089 Biotin_lipoyl

Graphical View of Domain Structure for InterPro Entry O00763

ProtoNet protein and cluster: O00763

2 Blocks protein families:

IPB001882 Biotin-requiring enzyme

IPB013537 Acetyl-CoA carboxylase

UniProtKB/Swiss-Prot: ACACB_HUMAN, O00763

Similarity: Contains 1 ATP-grasp domain

Similarity: Contains 1 biotin carboxylation domain

Similarity: Contains 1 biotinyl-binding domain

Similarity: Contains 1 carboxyltransferase domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: ACACB_HUMAN, O00763

Function: ACC-beta may be involved in the provision of malonyl-CoA or in the regulation of fatty acid oxidation, rather

than fatty acid biosynthesis. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and

carboxyltransferase

Catalytic activity: ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA

Catalytic activity: ATP + biotin-[carboxyl-carrier-protein] + CO(2) = ADP + phosphate +

carboxy-biotin-[carboxyl-carrier-protein]

Enzyme regulation: Activity is increased by oligomerization. Activated by citrate. Citrate and MID1IP1 promote

oligomerization. Inhibited by malonyl-CoA

Biophysicochemical properties: Kinetic parameters: KM=120 uM for ATP; KM=58 uM for Acetyl-CoA; KM=3.0 mM for NaHCO(3);

Genatlas biochemistry entry for ACACB:

acetyl-CoA carboxylase beta,280kDa,expressed in heart and skeletal muscle,liver,colocalizing with CPT1 at the outer

mitochondrial membrane,providing the malonyl-CoA allosterically regulating CPT1 activity,involved in the regulation of

fatty acid beta-oxidation and energy production

Enzyme Number (IUBMB): EC 6.4.1.2¹

miRNA Products:		OriGene 3'-UTR Clone: ACACB Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ACACB
		7 QIAGEN miScript miRNA Assays for microRNAs that regulate ACACB:
		hsa-miR-205 hsa-miR-125a-5p hsa-miR-670 hsa-miR-125b hsa-miR-3613-3p hsa-miR-1910 hsa-miR-4319
		SwitchGear 3'UTR luciferase reporter plasmid: ACACB 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ACACB (see all 7) OriGene shRNA RFP: ACACB OriGene siRNA: ACACB
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ACACB

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for ACACB

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ACACB (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ACACB (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: ACACB (NM_001093)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for ACACB
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ACACB

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for ACACB
		Search LifeMap BioReagents cell lines for ACACB

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ACACB

Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003989	acetyl-CoA carboxylase activity	IDA	--
GO:0004075	biotin carboxylase activity	IEA	--
GO:0005515	protein binding	IPI	16794074
GO:0005524	ATP binding	IEA	--
GO:0046872	metal ion binding	IEA	--

ACACB for ontologies           About GeneDecksing

Animal Models:
     Mouse knock-outs for ACACB: Acacb^tm1Sjw Acacb^tm1.2Lowl Acacb^tm1Dejs
     9 MGI mutant phenotypes (inferred from 4 alleles) (MGI details for Acacb):

adipose tissue	behavior/neurological	cardiovascular system	cellular	growth/size
homeostasis/metabolism	integument	muscle	normal

ACACB for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/16 super-pathways (see all 16) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Saturated fatty acid biosynthesis

Saturated fatty acid biosynthesis	1.00	biotin-carboxyl carrier protein assembly	0.50
Saturated fatty acid biosynthesis	1.00	Fatty acid biosynthesis	0.50
Transcriptional activation of Acetyl-CoA carboxylase by ChREBP:MLX	0.67	ChREBP activates metabolic gene expression	0.38

Metabolism

	Metabolism	1.00			Metabolism of lipids and lipoproteins	0.34
	Metabolic pathways	0.38

Development_Leptin signaling via PI3K-dependent pathway

	Development Leptin signaling via PI3K-dependent pathway	1.00			Adipocytokine signaling pathway	0.29
	Development_Leptin signaling via PI3K-dependent pathway	1.00

Regulation of Cholesterol Biosynthesis by SREBP (SREBF)

Regulation of Cholesterol Biosynthesis by SREBP (SREBF)

1.00

Activation of Gene Expression by SREBP (SREBF)

0.67

Glucose / Energy Metabolism

Glucose / Energy Metabolism

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

2 EMD Millipore Pathways for ACACB

	Saturated fatty acid biosynthesis
	Development Leptin signaling via PI3K-dependent pathway

1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for ACACB

AMPK Enzyme Complex Pathway

1 Cell Signaling Technology (CST) Pathway for ACACB

Glucose / Energy Metabolism

2 GeneGo (Thomson Reuters) Pathways for ACACB

	Development Leptin signaling via PI3K-dependent pathway
	Saturated fatty acid biosynthesis

3 BioSystems Pathways for ACACB

	AMPK signaling
	biotin-carboxyl carrier protein assembly
	Fatty Acid Biosynthesis

5/10 Reactome Pathways for ACACB (see all 10)

	Expression of Acetyl CoA Carboxylase 2 (ACACB, ACC2)
	Metabolism
	Transcriptional activation of Acetyl-CoA carboxylase by ChREBP:MLX
	Integration of energy metabolism
	Fatty acid, triacylglycerol, and ketone body metabolism

1 PharmGKB Pathway for ACACB

Metformin Pathway, Pharmacodynamic

5/6 Kegg Pathways (Kegg details for ACACB) (see all 6):

	Fatty acid biosynthesis
	Pyruvate metabolism
	Propanoate metabolism
	Metabolic pathways
	Insulin signaling pathway

UniProtKB/Swiss-Prot: ACACB_HUMAN, O00763

Pathway: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1

ACACB for pathways About GeneDecksing

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for ACACB

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/222 Interacting proteins for ACACB (O00763^{2, 3} ENSP00000367079⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 222)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
HLCS	P50747²^,³, ENSP00000338387⁴	MINT-7543053 MINT-7543129 MINT-7543070 MINT-7543096 I2D: score=2 STRING: ENSP00000338387
ENSG00000258947	Q13509³	I2D: score=1
MYC	P01106³, ENSP00000367207⁴	I2D: score=1 STRING: ENSP00000367207
NME1-NME2	P22392³	I2D: score=1
NME2	P22392³	I2D: score=1

About this table

Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006084	acetyl-CoA metabolic process	IDA	--
GO:0006112	energy reserve metabolic process	TAS	--
GO:0006633	fatty acid biosynthetic process	IEA	--
GO:0006853	carnitine shuttle	TAS	--
GO:0031325	positive regulation of cellular metabolic process	TAS	--

ACACB for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

ACACB for compounds About GeneDecksing

EMD Millipore small molecules for ACACB:
Small Molecule - inhibitor

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for ACACB

7 HMDB Compounds for ACACB About this table

Compound	Synonyms	CAS #	PubMed Ids
ADP	adenosindiphosphorsaeure (see all 8)	58-64-0	--
Acetyl-CoA	S-Acetyl coenzyme A (see all 13)	72-89-9	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Biotin	(+)-Biotin (see all 42)	58-85-5	--
Malonyl-CoA	Malonyl Coenzyme A (see all 11)	524-14-1	--
Manganese	manganese	7439-96-5	--
Phosphate	NFB Orthophosphate (see all 13)	14265-44-2	--

5 DrugBank Compounds for ACACB About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Adenine	1H-Purin-6-amine (see all 5)	73-24-5	target	--	17139284 17016423 12829626 8318018 2880560 10592235
Soraphen A	--	122547-72-2	target	--	17139284 17016423 10592235
(2S)-2-(4-{[3-CHLORO-5-(TRIFLUOROMETHYL)PYRIDIN-2-YL]OXY}PHENOXY)PROPANOIC ACID	--	--	target	--	10592235
2-[4-(2,4-Dichlorophenoxy)Phenoxy]Propanoic Acid	--	--	target	--	10592235
Biotin	(+)-cis-Hexahydro-2-oxo-1H-thieno[3,4]imidazole-4-valeric acid (see all 7)	58-85-5	target	--	17477831

9 Novoseek chemical compound relationships for ACACB gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
acetyl-coa	90.4	48	16721829 (2), 17223360 (2), 17267221 (1), 19832698 (1) (see all 29)
aicar	69.8	1	15356065 (1)
fatty acid	69.3	40	15769985 (3), 16142397 (2), 15356065 (2), 8876158 (2) (see all 16)
carnitine	52.2	2	8876158 (1)
sterol	49.1	1	17671740 (1)
lipid	31	1	15356065 (1)
glucose	26.9	7	16376850 (4), 16421342 (1), 18063403 (1)
atp	25.3	1	11832374 (1)
glycogen	20	2	11832374 (2)

Search CenterWatch for drugs/clinical trials and news about ACACB

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for ACACB gene:

NM_001093.3

Unigene Cluster for ACACB:

Acetyl-CoA carboxylase beta

Hs.234898 [show with all ESTs]

Unigene Representative Sequence: NM_001093

16 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000338432(uc001tob.3 uc001toc.3 uc001tod.3 uc010sxm.2)

ENST00000539864 ENST00000546328 ENST00000544726 ENST00000543080 ENST00000544651

ENST00000538526 ENST00000542524 ENST00000534852 ENST00000537347 ENST00000536440

ENST00000396233 ENST00000537279 ENST00000377848 ENST00000377854 ENST00000543201(uc010sxl.1)

miRNA Products:		OriGene 3'-UTR Clone: ACACB Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ACACB
		7 QIAGEN miScript miRNA Assays for microRNAs that regulate ACACB:
		hsa-miR-205 hsa-miR-125a-5p hsa-miR-670 hsa-miR-125b hsa-miR-3613-3p hsa-miR-1910 hsa-miR-4319
		SwitchGear 3'UTR luciferase reporter plasmid: ACACB 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ACACB (see all 7) OriGene shRNA RFP: ACACB OriGene siRNA: ACACB
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ACACB

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ACACB (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ACACB (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: ACACB (NM_001093)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for ACACB
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ACACB

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ACACB Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat ACACB
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ACACB
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ACACB

Additional cDNA sequence:

AB209110.1 AJ575431.2 AJ575592.3 AK302469.1 AK302502.1 AK307466.1 AY382667.1 BC009753.2

BC028417.1 BC133715.1 DQ493870.1 U34591.1

10 DOTS entries:

DT.97772780 DT.92430012 DT.97847648 DT.311823 DT.311822 DT.97847647 DT.100664378 DT.91668133

DT.99947442 DT.75190335

24/182 AceView cDNA sequences (see all 182):

CA406123 BQ694002 F04454 CA397438 CA407836 AI633820 BM710345 AI084970

CF455324 AI263610 AJ575431 BP334758 BG698086 AI918780 AJ575592 AY382667

AI969384 F06449 AI004903 AA912351 BQ708221 CB163592 CR626281 BC009753

GeneLoc Exon Structure

5/11 Alternative Splicing Database (ASD) splice patterns (SP) for ACACB (see all 11) About this scheme

ExUns:	1	^	2	^	3	^	4	^	5a	·	5b	^	6	^	7	^	8	^	9	^	10a	·	10b	^	11	^	12a	·	12b	·	12c	^	13a	·	13b	^	14a	·	14b	^	15	^	16	^	17a	·	17b	^	18	^	19	^
SP1:			-								-																				-
SP2:																															-								-		-		-		-
SP3:
SP4:
SP5:

ExUns:	20	^	21	^	22	^	23	^	24	^	25	^	26	^	27	^	28	^	29	^	30	^	31a	·	31b	^	32	^	33	^	34	^	35	^	36a	·	36b	^	37a	·	37b	^	38	^	39	^	40	^	41a	·	41b	^
SP1:																							-		-												-
SP2:																							-		-												-
SP3:																																					-
SP4:																											-										-
SP5:

ExUns:	42	^	43	^	44a	·	44b	^	45a	·	45b	^	46a	·	46b	^	47a	·	47b	^	48	^	49	^	50a	·	50b	^	51	^	52	^	53	^	54	^	55	^	56	^	57a	·	57b
SP1:					-						-														-				-
SP2:					-						-														-				-
SP3:					-						-														-
SP4:
SP5:																													-

ECgene alternative splicing isoforms for ACACB

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

ACACB expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AGGTTGCCCA

About this image

ACACB expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

1 LifeMap In Vivo Development Anatomical Compartment/Cell

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Heart

Left Ventricle

Heart

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

White adipocyte-like cells (Differentiation of ...)

Adipose

See ACACB Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ACACB

SOURCE GeneReport for Unigene cluster: Hs.234898

UniProtKB/Swiss-Prot: ACACB_HUMAN, O00763

Tissue specificity: Predominantly expressed in the heart, skeletal muscles and liver

SABiosciences Expression via Pathway-Focused PCR Arrays including ACACB:

Insulin Resistance in human mouse rat

Adipogenesis in human mouse rat

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In Situ
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(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for ACACB gene from 9/29 species (see all 29) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Acacb^{1 , 5}	acetyl-Coenzyme A carboxylase beta^{1, 5}	85.01(n)¹ 88.81(a)¹		5 (55.97 cM)⁵ 100705¹ NM_133904.2¹ NP_598665.2¹ 114146535⁵
chicken (Gallus gallus)	Aves	ACACB¹	acetyl-CoA carboxylase beta	76.5(n) 80.67(a)		430557 XM_428114.3 XP_428114.3
lizard (Anolis carolinensis)	Reptilia	ACACB⁶	--	75(a)	1 ↔ 1	1(118289679-118331307)
tropical clawed frog (Xenopus tropicalis)	Amphibia	BX699087.1²	--	72.89(n)		BX699087.1
zebrafish (Danio rerio)	Actinopterygii	acacb¹	acetyl-CoA carboxylase beta	72.31(n) 78.53(a)		556236 XM_678989.5 XP_684081.4
fruit fly (Drosophila melanogaster)	Insecta	CG11198³	acetyl-CoA carboxylase	59(a)		--
worm (Caenorhabditis elegans)	Secernentea	W09B6.1³	acetyl-CoA carboxylase	44(a) (best of 2)		II(1143941-1152380) --
thale cress (Arabidopsis thaliana)	eudicotyledons	PAS3⁶ ACC2⁶	acetyl-CoA carboxylase 2	37(a) 35(a)	possible ortholog possible ortholog	1(13533411-13544009) 1(13545965-13558492)
rice (Oryza sativa)	Liliopsida	-- --	acetyl-CoA carboxylase, putative, expressed	38(a) 35(a)	possible ortholog possible ortholog	10(11202713-11213820) 5(13007093-13021023)

ENSEMBL Gene Tree for ACACB (if available)
TreeFam Gene Tree for ACACB (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for ACACB gene

ACACA²

2 SIMAP similar genes for ACACB using alignment to 9 protein entries: ACACB_HUMAN (see all proteins):

ACACA MCCC1

ACACB for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 12 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs113159614^1,2	C,	--	106596568(+)	`CCTTCC/TTTCCT`	1	--	int¹		0	--	--	--	--
rs181817509^1,2		--	109575207(+)	`CGCCTC/GTAATC`	1	--	us2k¹		0	--	--	--	--
rs185824263^1,2		--	109575387(+)	`TTGAAA/CCCAGG`	1	--	us2k¹		0	--	--	--	--
rs138839784^1,2		--	109575421(+)	`GATTAC/TGCCAC`	1	--	us2k¹		0	--	--	--	--
rs190151644^1,2		--	109575422(+)	`ATTACA/GCCACT`	1	--	us2k¹		0	--	--	--	--
rs73197453^1,2	C,	--	109575477(+)	`AAAAAA/GGGATT`	1	--	us2k¹		0	--	--	--	--
rs74783014^1,2	F,	--	109575596(+)	`CCATTT/AAAAAA`	1	--	us2k¹		1		NA	120
rs73197454^1,2	C,	--	109575599(+)	`TTTAAA/CAATTT`	1	--	us2k¹		3		WA NA EA	242
rs3759389^1,2	C,	--	109575827(-)	`TACTCA/GGGAGT`	1	--	us2k¹		0	--	--	--	--
rs146597055^1,2		--	109575946(+)	`TCAGGC/TGATCT`	1	--	us2k¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for ACACB (109554400 - 109706031 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for ACACB
2 Indels: 24853 44968
Human Gene Mutation Database (HGMD): ACACB

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QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ACACB

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

ACACB for disorders           About GeneDecksing

OMIM gene information: 601557    OMIM disorders: --

17 diseases for ACACB:    About MalaCards

insulin resistance anorexia nervosa fatty liver disease liver disease

proteinuria obesity lung carcinoma pancreatic cancer

nephropathy prostate cancer pancreatitis cholesterol

hypoxia prostatitis carcinoma malaria

alcoholism

4 Novoseek disease relationships for ACACB gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
fatty liver	41.7	1	20139635 (1)
insulin resistance	40.1	1	15955844 (1)
insulin sensitivity	38.7	1	14627750 (1)
obesity	34.7	4	20439761 (1), 20139635 (1), 16721829 (1)

Human Genome Epidemiology (HuGE) Navigator: ACACB (15 documents)

Export disorders for ACACB gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for ACACB gene, integrated from 9 sources (see all 108):
(articles sorted by number of sources associating them with ACACB)

Utopia: connect your pdf to the dynamic
world of online information

Identification of a second human acetyl-CoA carboxylase gene. (PubMed id 8670171)^{1, 2, 3} Widmer J....Witters L.A. (1996)
Human acetyl-CoA carboxylase 2. Molecular cloning, characterization, chromosomal mapping, and evidence for two isoforms. (PubMed id 9099716)^{1, 2, 9} Abu-Elheiga L.... Wakil S.J. (1997)
Expression, purification, and characterization of human and rat acetyl coenzyme A carboxylase (ACC) isozymes. (PubMed id 16854592)^{1, 2, 9} Cheng D.... Tamura J.K. (2007)
ACC2 is expressed at high levels human white adipose and has an isoform with a novel N-terminus. (PubMed id 19190759)^{1, 9} Castle J.C....Johnson J.M. (2009)
Differential activation of recombinant human acetyl-CoA carboxylases 1 and 2 by citrate. (PubMed id 18455495)^{1, 9} Locke G.A....Marcinkeviciene J. (2008)
Cloning of human acetyl-CoA carboxylase-beta and its unique features. (PubMed id 8876158)^{1, 9} Ha J....Kim K.H. (1996)
A single nucleotide polymorphism within the acetyl-co enzyme A carboxylase beta gene is associated with proteinuria in patients with type 2 diabetes. (PubMed id 20168990)^{1, 9} Maeda S....Nakamura Y. (2010)
Alternative usages of multiple promoters of the acetyl-CoA carboxylase beta gene are related to differential transcriptional regulation in human and rodent tissues. (PubMed id 15590647)^{1, 9} Oh S.Y....Kim K.S. (2005)
Hormonal regulation of acetyl-CoA carboxylase isoenzy me gene transcription. (PubMed id 20139635)^{1, 9} Zhao L.F....Terada Y. (2010)
Dissociation of AMPK activity and ACCbeta phosphorylation in human muscle during prolonged exercise. (PubMed id 12413941)^{1, 9} Wojtaszewski J.F....Pilegaard H. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 32	HGNC: 85	AceView: ACACB	Ensembl:ENSG00000076555	euGenes: HUgn32
ECgene: ACACB	Kegg: 32	H-InvDB: ACACB

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for ACACB	Pharmacogenomics, SNPs, Pathways

Intellectual Property for ACACB gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for ACACB gene:
Search GeneIP for patents involving ACACB

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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Advanced Cell Diagnostics
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acetyl-CoA carboxylase beta

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acetyl-CoA carboxylase beta

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