ABHD4 Gene
protein-coding GIFtS: 47
GCID: GC14P023067
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|
abhydrolase domain containing 4
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Aliases
for ABHD4 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Abhydrolase Domain Containing 41 2 | | ABH42 | | Alpha/Beta-Hydrolase 42 3 | | Abhydrolase Domain-Containing Protein 42 | | Lyso-N-Acylphosphatidylethanolamine Lipase2 3 | | EC 3.1.1.-3 | | FLJ128161 | | |
Export aliases for ABHD4 gene to outside databasesPrevious GC identifers: GC14P016854 GC14P021057 GC14P022136 GC14P003185 |
Summaries
for ABHD4 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: ABHD4_HUMAN, Q8TB40Function: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine (By similarity)
|
Genomic Views
for ABHD4 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000014.8 NC_018925.1 NT_026437.12
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the ABHD4 gene promoter:
TBP Sp1 p53 Pax-2 Pax-2a POU3F2 CREB Pax-2b deltaCREB Chx10
Other transcription factors
Search SABiosciences Chromatin IP Primers for ABHD4
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ABHD4 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 14q11.2 Ensembl cytogenetic band: 14q11.2 HGNC cytogenetic band: 14q11.1ABHD4 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 14 GeneLoc Exon Structure GeneLoc location for GC14P023067: view genomic region
(about GC identifiers)
Start:
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23,067,146 bp from pter |
End:
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23,081,265 bp from pter |
Size:
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14,120 bases |
Orientation:
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plus strand |
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Proteins
for ABHD4 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: ABHD4_HUMAN, Q8TB40 (See
protein sequence)Recommended Name: Abhydrolase domain-containing protein 4 Size: 342 amino acids; 38794 Da
Caution: Thr-291 is present instead of the conserved His which is expected to be an active site residue
Secondary accessions: Q9H9E0Explore the universe of human proteins at neXtProt for ABHD4: NX_Q8TB40
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8TB40
ABHD4 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_071343.2
ENSEMBL proteins: ENSP00000414558 ENSP00000216327 ENSP00000438332 ENSP00000437385 ENSP00000388751 ENSP00000444550 ENSP00000438014
Human Recombinant Protein Products:
ABHD4 Antibody Products:
Assay Products for ABHD4: |
Protein
Domains /
Families
for ABHD4 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
ABHD4 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q8TB40ProtoNet protein and cluster: Q8TB40 2 Blocks protein families: IPB000073 Alpha/beta hydrolase fold IPB002410 Prolyl aminopeptidase (S33) family signature
UniProtKB/Swiss-Prot: ABHD4_HUMAN, Q8TB40Similarity: Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily |
Function
for ABHD4 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: ABHD4_HUMAN, Q8TB40Function: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine (By similarity)Enzyme Number (IUBMB): EC 3.1.1.-1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ABHD4 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ABHD4
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: ABHD4 (NM_022060) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for ABHD4 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ABHD4 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ABHD4 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016787 | hydrolase activity |
IEA | -- |
ABHD4 for ontologies About GeneDecksing
|
Pathways & Interactions
for ABHD4 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ABHD4
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
4 Interacting proteins for ABHD4 (Q8TB402, 3 ENSP000002163274) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016042 | lipid catabolic process |
IEA | -- |
ABHD4 for ontologies About GeneDecksing
|
Drugs & Compounds
for ABHD4 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for ABHD4
10/1076 HMDB Compounds for ABHD4 (see all 1076) About this table
Search CenterWatch for drugs/clinical trials and news about ABHD4
|
Transcripts
for ABHD4 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for ABHD4 gene: NM_022060.2 Unigene Cluster for ABHD4: Abhydrolase domain containing 4 Hs.445665 [show with all ESTs]Unigene Representative Sequence: NM_02206010 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000428304(uc010tna.1 uc001wgm.3 uc010tnb.2) ENST00000216327
ENST00000537243 ENST00000544562 ENST00000542041 ENST00000539344(uc010tmz.1)
ENST00000418446 ENST00000545034 ENST00000541962 ENST00000489928
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ABHD4 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ABHD4
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: ABHD4 (NM_022060) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for ABHD4 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ABHD4 |
Additional cDNA sequence: AK022878.1 AK024790.1 AK293198.1 AK298127.1 AK301315.1 BC024779.1 7 DOTS entries: DT.111772 DT.402733 DT.95271266 DT.102831536 DT.120746454 DT.92387876 DT.120746442 24/146 AceView cDNA sequences (see all 146): NM_022060 BM762794 BI829156 BU507199 BM802713 AI668845 CD518051 CR610790 BU192139 BM466288 CB216203 BM558186 CF272334 BM977681 AI089359 BQ933353 BX393729 BM713221 CD559115 AL041951 BM996134 CN484948 CB529668 C01492
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for ABHD4 About this scheme
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | · | 4c | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 |
|---|
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| SP1: | | | | | - | | | | | | | | - | | | | - | | | | | | | | |
| SP2: | | | | | - | | | | | | | | - | | - | | - | | | | | | | | |
| SP3: | | | | | - | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | - | | | | | | | | | | | |
ECgene alternative splicing isoforms for ABHD4
|
Expression
for ABHD4 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| ABHD4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTTTCCAGCT
About this image
See ABHD4 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ABHD4
SOURCE GeneReport for Unigene cluster: Hs.445665
SABiosciences Custom PCR Arrays for ABHD4
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ABHD4
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat ABHD4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ABHD4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ABHD4 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ABHD4 |
Orthologs
for ABHD4 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for ABHD4 gene from 9/29 species (see all 29) About this table
ENSEMBL Gene Tree for ABHD4 (if available)
TreeFam Gene Tree for ABHD4 (if available) |
Paralogs
for ABHD4 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for ABHD4 gene
- ABHD52
1 SIMAP similar gene for ABHD4 using alignment to 7 protein entries: ABHD4_HUMAN (see all proteins):ABHD5
ABHD4 for paralogs About GeneDecksing
|
Genomic Variants
for ABHD4 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
| | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases
for ABHD4 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
| -- |
Publications
for ABHD4 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for ABHD4 gene, integrated from 9 sources (see all 11):
(articles sorted by number of sources associating them with ABHD4) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- A genome wide shRNA screen identifies a/b hydrolase do main containing 4 (ABHD4) as a novel regulator of anoikis resistance. (PubMed id 22488300)1 Simpson C.D....Schimmer A.D. (2012)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway. (PubMed id 16818490)1 Simon G.M. and Cravatt B.F. (2006)
- Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures. (PubMed id 12486725)6 Kurz M....Kramer W. (2003)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
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External Searches for ABHD4 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing ABHD4 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing ABHD4 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing ABHD4 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for ABHD4 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for ABHD4 gene:
Search GeneIP for patents involving ABHD4
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for ABHD4 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for ABHD4 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ABHD4 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ABHD4 | | | OriGene Protein Over-expression Lysate for ABHD4 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for ABHD4 | | OriGene 3'-UTR Clone for ABHD4 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ABHD4 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ABHD4 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for ABHD4 | | OriGene Custom Protein Services for ABHD4 | | | OriGene Custom Immunoassay Development | | |
| |
 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat ABHD4 | | | Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ABHD4 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ABHD4 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ABHD4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ABHD4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ABHD4 |
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| | | | Search Tocris compounds for ABHD4 |
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 | | | ABHD4 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ABHD4 |
|  |  |  | | | | Search ThermoFisher Antibodies for ABHD4 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ABHD4 |
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