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ABCC12 Gene

protein-coding   GIFtS: 51

GC16M046674
ATP-binding cassette, sub-family C (CFTR/MRP), member 12
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
MGC27071 2
MRP9 1, 2, 3, 5
OTTHUMP00000164186 2
Descriptions
ATP-binding cassette protein C12 2
ATP-binding cassette transporter sub-family C member 12 2, 3
ATP-binding cassette, sub-family C (CFTR/MRP), member 12 2
multidrug resistance-associated protein 9 2
External Ids
HGNC: 146401
Entrez Gene: 941602
UniProtKB: Q96J653
Ensembl: ENSG000001407987
Search outside databases for aliases for ABCC12 gene

Previous GC identifers: GC16M038531 GC16M048165 GC16M047854 GC16M047894

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ABCC12:
This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded
protein contains two ATP-binding domains and 12 transmembrane regions. ABC proteins transport
various molecules across extra- and intracellular membranes. ABC genes are divided into seven
distinct subfamilies: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White. This gene is a member of
the MRP subfamily which is involved in multi-drug resistance. This gene and another subfamily
member are arranged head-to-tail on chromosome 16q12.1. Increased expression of this gene is
associated with breast cancer. [provided by RefSeq]

UniProtKB/Swiss-Prot: MRP9_HUMAN, Q96J65
Function: Probable transporter (By similarity)

Gene Wiki entry for ABCC12

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ABCC12 gene  

Entrez Gene cytogenetic band: 16q12.1   Ensembl cytogenetic band:  16q12.1   HGNC cytogenetic band: 16q12.1

ABCC12 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M046674:     (about GC identifiers)

Start:
46,674,385 bp from pter
End:
46,738,182 bp from pter
Size:
63,798 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000016.8  NT_010498.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: MRP9_HUMAN, Q96J65 (See protein sequence)
Recommended Name: Multidrug resistance-associated protein 9  
Size: 1359 amino acids; 152297 Da
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
Developmental stage: Expressed in fetal tissues
Secondary accessions: Q49AL2 Q8TAF0 Q8TEY2
Alternative splicing: 5 isoforms:  Q96J65-1   Q96J65-2   Q96J65-3   Q96J65-4   Q96J65-5   

REFSEQ proteins: NP_150229.2  

ENSEMBL proteins: 
ENSP00000311030 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (ABCC12)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
About this table

Antibodies for ABCC12: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (ABCC12), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for ABCC12

Assays for ABCC12: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5 InterPro domains/families:
 IPR003593 ATPase_AAA+_core
 IPR003439 ABC_transporter-like
 IPR017940 ABC_transporter_type1
 IPR001140 ABC_TM_transpt
 IPR017871 ABC_transporter_CS


   GeneDecks  ABCC12 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q96J65

ProtoNet protein and cluster: Q96J65

UniProtKB/Swiss-Prot: MRP9_HUMAN, Q96J65
Similarity: Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily
Similarity: Contains 2 ABC transmembrane type-1 domains
Similarity: Contains 2 ABC transporter domains

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ABCC12)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ABCC12)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_033226

              Applied Biosystems Silencer® siRNAs for ABCC12

              Sigma-Aldrich siRNA and siRNA Panels for ABCC12  
                     Sigma-Aldrich shRNA for ABCC12  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_033226
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_033226
                                 untagged cDNA clone in CMV expression vector: NM_033226 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_033226

UniProtKB/Swiss-Prot: MRP9_HUMAN, Q96J65
Function: Probable transporter (By similarity)

1 MGI mutant phenotype (inferred from 1 allele(MGI details for Abcc12):

no phenotypic analysis

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0005524 ATP binding IEA--
GO:0016887 ATPase activity IEA--
GO:0042626 ATPase activity, coupled to transmembrane movement of substances IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Kegg Pathway  (Kegg details for ABCC12):
 hsa02010 ABC transporters

   GeneDecks  ABCC12 for the pathways selected above  
About GeneDecksing

1 Interacting protein for ABCC12 (ENSP000003110303) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
MRPS7ENSP000002455393STRING (score=.764)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006810 transport IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ABCC12

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ABCC12)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ABCC12)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_033226

              Sigma-Aldrich siRNA and siRNA Panels for ABCC12  
                     Sigma-Aldrich shRNA for ABCC12  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_033226  

REFSEQ mRNAs for ABCC12 gene: 

NM_033226.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_033226  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_033226
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_033226
                                 untagged cDNA clone in CMV expression vector: NM_033226 

Additional cDNA sequence: 

AK122635.1 AK302520.1 AK307109.1 AK308599.1 AY040220.1 

7 DOTS entries:

DT.95099023  DT.101960525  DT.97835594  DT.87014331  DT.100749891  DT.101982304  DT.91992476 

20 AceView cDNA sequences:

NM_145188 NM_033226 NM_145189 AY196326 AY040220 AK122635 NM_145190 BC036378 
AK127951 NM_145187 BX646798 AF395908 AF395909 BG723063 AF411578 AA723717 
AF411577 AW341683 CD637552 AA970140 

highest scoring ESTs for ABCC12:

AY040220 BG723063 AA723717 AA970140 AF395908 AF395909 AF411577 AF411578 AK122635 AY196326 

Unigene Cluster for ABCC12:

ATP-binding cassette, sub-family C (CFTR/MRP), member 12
Hs.410111  [show with all ESTs]
Unigene Representative Sequence: AY040220


GeneLoc Exon Structure

5/11 Alternative Splicing Database (ASD) splice patterns (SP) for ABCC12 (see all 11 )

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16a · 16b ^ 17 ^ 18 ^ 19 ^ 20 ^
SP1:                                                                                      -                                                                     
SP2:                                                                                      -                             -                                       
SP3:                                                                                      -                             -                                       
SP4:                                                                                      -                             -                                       
SP5:                                                                                      -                             -                                   -   

ExUns: 21 ^ 22a · 22b ^ 23a · 23b ^ 24 ^ 25a · 25b · 25c ^ 26 ^ 27 ^ 28 ^ 29 ^ 30a · 30b · 30c
SP1:        -           -                             -           -                                 
SP2:                    -                             -           -                                 
SP3:                    -                       -     -     -     -                                 
SP4:                    -                             -     -     -                                 
SP5:                    -                       -     -           -                                 

About this scheme

ECgene alternative splicing isoforms for ABCC12

1 Ensembl transcript including schematic representation:
ENST00000311303  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ABCC12 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for ABCC12

1 / 2 / 3

2 probe-sets matching ABCC12 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

1552590_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1553410_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GGAAAATGCC

SOURCE GeneReport for Unigene cluster: Hs.410111

UniProtKB/Swiss-Prot: MRP9_HUMAN, Q96J65
Tissue specificity: Expressed in testis (at protein level). Widely expressed at low level. Isoform
5 is specifically expressed in brain, testis and breast cancer cells

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for ABCC12 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
ABCC121   -- ATP-binding cassette, sub-family C (CFTR/MRP), member more 88.49(n)
88.63(a)
487294  XM_544420.2  XP_544420.2 
chimpanzee
(Pan troglodytes)
ABCC121   -- ATP-binding cassette, sub-family C (CFTR/MRP), member more 96.76(n)
95.93(a)
745745  XM_001163361.1  XP_001163361.1 
rat
(Rattus norvegicus)
Abcc121   -- ATP-binding cassette, sub-family C (CFTR/MRP), member more 83.84(n)
84.43(a)
291923  NM_199377.1  NP_955409.1 
mouse
(Mus musculus)
Abcc121, 5 8 (42.00 cM)5
ATP-binding cassette, sub-family C (CFTR/MRP), member more1, 5 84.5(n)1
85.46(a)1
2445621  NM_172912.41  NP_766500.31 
 AF5021465  AF5144145  (see all 14)
zebrafish
(Danio rerio)
LOC5684821   -- similar to ATP-binding cassette protein C12 59.17(n)
54.39(a)
568482  XM_691812.3  XP_696904.3 
About this table        Species with no ortholog for ABCC12

ENSEMBL Gene Tree for ABCC12
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for ABCC12 gene
ABCC112  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/252 NCBI SNPs in ABCC12 are shown (see all 252 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 117)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 16 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs65003051,2
A,C,F,H46692357(+) GTGGGT/AGAACC 1 S/T mis19Minor allele frequency- A:0.09EU EA WA NA 912
rs71939551,2
A,C,F,H46680083(+) CCCACA/GGCTGG 1 R/C mis1 ese313Minor allele frequency- G:0.34EU EA WA NA 1272
rs65003041,2
A,C,F,H46692285(+) TCACTA/GGGTGA 1 Y/H mis18Minor allele frequency- G:0.08EU EA WA 836
rs71935311,2
A,F,H46740014(+) TGAGAG/ATATTA 1 -- ng314Minor allele frequency- A:0.09EU EA WA 408
rs169458161,2
C,F,H46708699(+) GAGGTT/AGCTCA 1 Y/N mis1 ese312Minor allele frequency- A:0.04NA EU EA WA 1030
rs123731051,2
C,F,H46675160(+) ATCGCA/TAATGC 1 F/L mis18Minor allele frequency- T:0.01EU EA WA 840
rs169457871,2
C,F,H46679401(+) GGTCTT/GCCAAG 1 A/E mis18Minor allele frequency- G:0.02NA EU EA WA 622
rs169458741,2
C,F,H46732736(+) CCCTTG/TCTACC 1 A/E mis116Minor allele frequency- T:0.15NA EU EA WA 1478
rs80574741,2
C,F,H46695773(+) GGCTCT/ATTAAG 1 M/K mis19Minor allele frequency- A:0.03EU EA WA NA 916
rs169459011,2
C,F,H46737812(+) TGAGAT/GAAGGT 1 L/I mis112Minor allele frequency- G:0.04NA EU EA WA 1048
About this table

HapMap Linkage Disequilibrium images for ABCC12 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607041

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/13 PubMed articles for ABCC12 gene (see all 13 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 94160 HGNC: 14640 AceView: ABCC12 Ensembl:ENSG00000140798 euGenes: HUgn94160
ECgene: ABCC12 H-InvDB: ABCC12
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for ABCC12:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Lentivirus-delivered shRNAs for ABCC12 Browse small molecules at Sigma-Aldrich
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