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AARS2 Gene

protein-coding   GIFtS: 47

GC06M044376
alanyl-tRNA synthetase 2, mitochondrial (putative)
(Previous name: alanyl-tRNA synthetase like )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: AARSL)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AARSL 2, 3
AlaRS 3
EC 6.1.1.7 3
KIAA1270 1, 2, 3, 5
MT-ALARS 2
MTALARS 2, 5
OTTHUMP00000016528 2
OTTHUMP00000039883 2
bA444E17.1 1, 2
Descriptions
Alanine--tRNA ligase 3
Alanyl-tRNA synthetase-like 3
alanine tRNA ligase 2, mitochondrial (putative) 2
alanyl-tRNA synthetase 2, mitochondrial 2
alanyl-tRNA synthetase 2, mitochondrial (putative) 2
alanyl-tRNA synthetase like 1, 2
External Ids
HGNC: 210221
Entrez Gene: 575052
UniProtKB: Q5JTZ93
Ensembl: ENSG000001246087
Search outside databases for aliases for AARS2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6p21.1   Ensembl cytogenetic band:  6p21.1   HGNC cytogenetic band: 6p21.1

AARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M044376:     (about GC identifiers)

Start:
44,374,441 bp from pter
End:
44,389,041 bp from pter
Size:
14,601 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000006.10  NT_007592.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYAM_HUMAN, Q5JTZ9 (See protein sequence)
Recommended Name: Probable alanyl-tRNA synthetase, mitochondrial precursor  
Size: 985 amino acids; 107340 Da
Subcellular location: Mitochondrion matrix (By similarity)
Secondary accessions: A2RRN5 Q8N198 Q96D02 Q9ULF0

REFSEQ proteins: NP_065796.1  

ENSEMBL proteins: 
ENSP00000244571 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Alanyl-tRNA synthetase 2)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion IEA--
GO:0005759 mitochondrial matrix IEA--
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Antibodies for AARS2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (Alanyl-tRNA synthetase 2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (AARS2)
Search Novus for antibodies for AARS2

Assays for AARS2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR002318 Ala-tRNA-synth_IIc
 IPR018164 Ala-tRNA-synth_IIc_N
 IPR012947 tRNA_SAD
 IPR018165 Ala-tRNA-synth_IIc_cons-reg


   GeneDecks  AARS2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q5JTZ9

ProtoNet protein and cluster: Q5JTZ9

1 Blocks protein family: IPB002318 Alanyl-tRNA synthetase signature

UniProtKB/Swiss-Prot: SYAM_HUMAN, Q5JTZ9
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (AARS2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020745

              Applied Biosystems Silencer® siRNAs for AARS2

              Sigma-Aldrich siRNA for AARS2  
                     Sigma-Aldrich shRNA for AARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020745
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020745
                                 untagged cDNA clone in CMV expression vector: NM_020745 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_020745

UniProtKB/Swiss-Prot: SYAM_HUMAN, Q5JTZ9
Catalytic activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Enzyme Number (IUBMB): EC 6.1.1.7 

5 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003676 nucleic acid binding IEA--
GO:0004813 alanine-tRNA ligase activity IEA--
GO:0005524 ATP binding IEA--
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


2 Sigma-Aldrich "Your Favorite Gene" Pathways for  AARS2  (Your Favorite Gene powered by Ingenuity) 
 Alanine and Aspartate Metabolism
 Aminoacyl-tRNA Biosynthesis

   GeneDecks  AARS2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for AARS2):
 hsa00252 Alanine and aspartate metabolism
 hsa00970 Aminoacyl-tRNA biosynthesis

   GeneDecks  AARS2 for the pathways selected above  
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5/9 Interacting proteins for AARS2 (ENSP000002445713) via UniProtKB, MINT, and/or STRING (see all 9 )
InteractantInteraction Details
GeneCardExternal ID(s)
AGXTENSP000003026203STRING (score=.914)
AGXT2ENSP000002314203STRING (score=.899)
GPTENSP000003468213STRING (score=.899)
GPT2ENSP000003452823STRING (score=.899)
AARSENSP000002617723STRING (score=.818)
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3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006412 translation IEA--
GO:0006419 alanyl-tRNA aminoacylation IEA--
GO:0043039 tRNA aminoacylation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for AARS2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (AARS2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020745

              Sigma-Aldrich siRNA for AARS2  
                     Sigma-Aldrich shRNA for AARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_020745  

REFSEQ mRNAs for AARS2 gene: 

NM_020745.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_020745  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020745
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020745
                                 untagged cDNA clone in CMV expression vector: NM_020745 

Additional cDNA sequence: 

AB033096.1 BC013593.1 BC021131.1 BC033169.1 BC041026.1 BC128175.1 BC131728.1 CR612653.1 

7 DOTS entries:

DT.110699  DT.208413  DT.95376032  DT.100025489  DT.121344622  DT.91953897  DT.99927375 

24/121 AceView cDNA sequences (see all 121 ):

BI789129 BQ682453 CK821197 AI806436 BQ883770 BM042314 BU146586 CR612653 
AI381515 BI769677 C01141 BQ642175 BI770414 AI183453 BQ925080 BM545479 
AB033096 BI859749 BE794883 BE464558 BU174518 AA627815 CA312977 AI942297 

Unigene Cluster for AARS2:

Alanyl-tRNA synthetase 2, mitochondrial (putative)
Hs.158381  [show with all ESTs]
Unigene Representative Sequence: NM_020745


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000244571  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

AARS2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for AARS2

1 / 2

7 probe-sets matching AARS2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
75455_at2 U95-E 1 1.00 1.00 0.71 0.90 -- -- -- -- --
69408_at2 U95-E 1 1.00 1.00 0.86 0.97 -- -- -- -- --
53488_at2 U95-B 1 1.00 1.00 0.78 1.14 -- -- -- -- --

231845_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

230024_at2 U133-B 1 0.91 1.00 -- -- -- -- -- -- --

231845_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

230024_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
GeneDecks  AARS2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.158381
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for AARS2 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
AARS21   -- alanyl-tRNA synthetase 2, mitochondrial (putative) 88.19(n)
87.41(a)
474920  XM_532155.2  XP_532155.2 
chimpanzee
(Pan troglodytes)
AARS21   -- alanyl-tRNA synthetase 2, mitochondrial (putative) 99.73(n)
99.59(a)
462733  XM_518510.2  XP_518510.2 
cow
(Bos taurus)
AARS21   -- alanyl-tRNA synthetase 2, mitochondrial (putative) 88.2(n)
87.49(a)
786099  XM_001253239.1  XP_001253240.1 
rat
(Rattus norvegicus)
Aars21   -- alanyl-tRNA synthetase 2, mitochondrial (putative) 83.13(n)
84.39(a)
301254  XM_236942.4  XP_236942.4 
mouse
(Mus musculus)
Aars21, 5 175
alanyl-tRNA synthetase 2, mitochondrial (putative)1, 5 83.2(n)1
83.57(a)1
2248051  NM_198608.21  NP_941010.21 
 AK1469585  AK1677715  (see all 17)
About this table        Species with no ortholog for AARS2

ENSEMBL Gene Tree for AARS2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for AARS2 gene
AARS2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/75 NCBI SNPs in AARS2 are shown (see all 75 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 63)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 6 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs93950021,2
A,C,F,H44389862(+) TTTCCA/GTAGTA 1 -- ng31 tfbs315Minor allele frequency- G:0.15NA EU EA WA 1400
rs93492791,2
A,C,F,H44389878(+) TGTGAC/ACCTGA 1 -- ng3111Minor allele frequency- A:0.16NA EU EA WA 978
rs47147761,2
A,C,F,H44390294(+) CAGTGT/GTTCTG 1 -- ng314Minor allele frequency- G:0.16EU EA WA 420
rs64583771,2
A,C,F,H44390261(+) TTTGAA/GAGTGT 1 -- ng314Minor allele frequency- G:0.01EU EA WA 418
rs3241361,2
A,C,F,H44382989(-) ATGGCG/ATCTTC 1 I/V mis1 ese39Minor allele frequency- A:0.10MN NA EU EA WA 808
rs3250091,2
C,F,H44376113(-) CCTACA/GTGACA 1 -- ut31 ese39Minor allele frequency- G:0.11MN EU EA WA 1020
rs3241351,2
C,F,H44389579(-) CAAGGG/AAACTG 1 -- ng314Minor allele frequency- A:0.01EU EA WA 418
--
rs77631881,2
A,F,H44390586(+) gcctaC/Actcag 1 -- ng314Minor allele frequency- A:0.01EU EA WA 412
rs172095601,2
C,F,H44389694(+) GTCTTC/TTCATT 1 -- ng3112Minor allele frequency- N:0.01NA EU EA WA 1054
rs4952941,2
C,H44380897(+) GCTCAG/TCCTGC 1 A/D mis14Minor allele frequency- T:0.00EU EA WA 420
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HapMap Linkage Disequilibrium images for AARS2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 612035

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

8 PubMed articles for AARS2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 57505 HGNC: 21022 AceView: AARSLandMGC33600 Ensembl:ENSG00000124608 euGenes: HUgn57505
ECgene: AARS2 H-InvDB: AARS2
(According to HUGE)
About This Section

HUGE: KIAA1270
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for AARS2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for AARS2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for AARS2 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for AARS2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
(Alanyl-tRNA synthetase 2)
 Antibodies (Alanyl-tRNA synthetase 2)
 Search OriGene for AARS2
 Search Tocris compounds for AARS2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for AARS2

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