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AARS Gene

protein-coding   GIFtS: 63

GC16M068843
alanyl-tRNA synthetase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AlaRS 3
EC 6.1.1.7 3
Descriptions
Alanine--tRNA ligase 3
Renal carcinoma antigen NY-REN-42 3
alanine tRNA ligase 1, cytoplasmic 2
alanyl-tRNA synthetase 2
External Ids
HGNC: 201
Entrez Gene: 162
UniProtKB: P495883
Ensembl: ENSG000000908617
Search outside databases for aliases for AARS gene

Previous GC identifers: GC16M060988 GC16M070706 GC16M070021 GC16M070062

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for AARS:
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II
enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is
reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht
the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific
aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a
catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a
second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AARS gene  

Entrez Gene cytogenetic band: 16q22   Ensembl cytogenetic band:  16q22.1   HGNC cytogenetic band: 16q22

AARS Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M068843:     (about GC identifiers)

Start:
68,843,798 bp from pter
End:
68,880,913 bp from pter
Size:
37,116 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000016.8  NT_010498.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SYAC_HUMAN, P49588 (See protein sequence)
Recommended Name: Alanyl-tRNA synthetase, cytoplasmic  
Size: 968 amino acids; 106810 Da
Subunit: Monomer
Subcellular location: Cytoplasm (Potential)
Secondary accessions: A6NF14 Q53GV7 Q96FA0

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_001596.2  

    ENSEMBL proteins: 
    ENSP00000261772 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (AlaRS)
    Human Recombinant Proteins from Abnova (AARS)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625 soluble fraction TAS7654687
    GO:0005737 cytoplasm TAS7654687
    About this table

    Antibodies for AARS: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for AARS
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (AlaRS), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (AARS)
    Novus Biologicals Antibody for AARS

    Assays for AARS: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR002318 Ala-tRNA-synth_IIc
     IPR018164 Ala-tRNA-synth_IIc_N
     IPR012947 tRNA_SAD
     IPR003156 Pesterase_DHHA1
     IPR018165 Ala-tRNA-synth_IIc_cons-reg


       GeneDecks  AARS for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P49588

    ProtoNet protein and cluster: P49588

    2 Blocks protein families:
    IPB002318 Alanyl-tRNA synthetase signature
    IPB003156 Phosphoesterase


    UniProtKB/Swiss-Prot: SYAC_HUMAN, P49588
    Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AARS)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (AARS)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001605

                  Applied Biosystems Silencer® siRNAs for AARS

                  Sigma-Aldrich siRNA for AARS  
                         Sigma-Aldrich shRNA for AARS  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for AARS
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001605
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001605
                                     untagged cDNA clone in CMV expression vector: NM_001605 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001605

    UniProtKB/Swiss-Prot: SYAC_HUMAN, P49588
    Catalytic activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
    Enzyme Number (IUBMB): EC 6.1.1.7 

    Genatlas biochemistry entry for AARS:
    alanyl-tRNA synthetase,target autoantibodies in the immune disease polymyositis/dermatomyositis

    3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Aars):

    behavior/neurologicalnervous systemskin/coat/nails

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000049 tRNA binding TAS7654687
    GO:0000166 nucleotide binding IEA--
    GO:0003676 nucleic acid binding IEA--
    GO:0004813 alanine-tRNA ligase activity IEA--
    GO:0005524 ATP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  AARS  (Your Favorite Gene powered by Ingenuity) 
     Aminoacyl-tRNA Biosynthesis
     Alanine and Aspartate Metabolism

       GeneDecks  AARS for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for AARS):
     hsa00252 Alanine and aspartate metabolism
     hsa00970 Aminoacyl-tRNA biosynthesis

       GeneDecks  AARS for the pathways selected above  
    About GeneDecksing

    5/24 Interacting proteins for AARS (ENSP000002617723 P495882) via UniProtKB, MINT, and/or STRING (see all 24 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HSP90AB1P082382MINT-72669
    YWHAZP631042MINT-3318644
    KARSENSP000003254483STRING (score=.946)
    ADAT1ENSP000003100153STRING (score=.935)
    AGXTENSP000003026203STRING (score=.921)
    About this table

    5/12 Gene Ontology (GO) biological process terms (links to tree view) (see all 12 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001942 hair follicle development IEA--
    GO:0006400 tRNA modification IEA--
    GO:0006412 translation IEA--
    GO:0006419 alanyl-tRNA aminoacylation TAS7761427
    GO:0006457 protein folding IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for AARS
    5 Novoseek chemical compound relationships for AARS gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    aminoacyl-trna 94.06 29 8286437 (1), 9358165 (1), 11310981 (1), 12421319 (1) (see all 23)
    trna 85.61 57 17704131 (3), 12554667 (2), 18437305 (2), 17726052 (2) (see all 25)
    anticodon 73.68 5 8286437 (1), 11310981 (1), 18611382 (1), 18322459 (1)
    smpb 72.91 2 11904185 (1), 16381838 (1)
    atp 0.00 2 8422978 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AARS)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (AARS)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001605

                  Sigma-Aldrich siRNA for AARS  
                         Sigma-Aldrich shRNA for AARS  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001605  

    REFSEQ mRNAs for AARS gene: 

    NM_001605.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001605  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001605
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001605
                                     untagged cDNA clone in CMV expression vector: NM_001605 

    Additional cDNA sequence: 

    AK222824.1 AK225116.1 AK299098.1 AK307782.1 BC011451.1 D32050.1 

    22 DOTS entries:

    DT.448485  DT.95262752  DT.100774719  DT.95262736  DT.95262747  DT.95262749  DT.428242  DT.95262734 
    DT.100034176  DT.100834181  DT.120712453  DT.40115644  DT.40115645  DT.80100249  DT.91960082  DT.92454788 
    DT.92454789  DT.95090763  DT.95262748  DT.99987249  DT.120712433  DT.91904558 

    24/635 AceView cDNA sequences (see all 635 ):

    BI258486 BQ933535 BQ058121 CD724013 BM472171 CD515330 BX423230 BP357328 
    BU541395 BM806818 BF794496 AI567786 AA099496 AL135343 BP357908 BQ057450 
    BU782759 BQ682930 BE897229 D32050 AW087287 BM756898 BQ682132 BG177128 

    highest scoring ESTs for AARS:

    D32050 AA135099 AL120911 AL515873 AL525814 AU098908 AU133772 AU143575 AW249176 AW500508 

    Unigene Cluster for AARS:

    Alanyl-tRNA synthetase
    Hs.315137  [show with all ESTs]
    Unigene Representative Sequence: AK222824


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for AARS (see all 6 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13a · 13b · 13c ^ 14 ^ 15a · 15b ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20a · 20b ^
    SP1:              -                                                     -                                                                                   -   
    SP2:                                                                                                                    -                                   -   
    SP3:                                                                                                                                                            
    SP4:                                                                                                                    -                                       
    SP5:                                                                                                                                                            

    ExUns: 21 ^ 22a · 22b
    SP1:                  
    SP2:                  
    SP3:                  
    SP4:                  
    SP5:                  

    About this scheme

    ECgene alternative splicing isoforms for AARS

    1 Ensembl transcript including schematic representation:
    ENST00000261772  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    AARS expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for AARS

    1 / 2 / 3

    3 probe-sets matching AARS gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    36185_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D32050 1.00 1.00 1.00 1

    201000_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001605 0.60 1.00 0.82 1

    201000_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTCTTCACGG

    SOURCE GeneReport for Unigene cluster: Hs.315137

    Expression variation in blood from EXPOLDB for AARS

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for AARS gene from 5/26 species (see all 26 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    AARS1   -- alanyl-tRNA synthetase 90.98(n)
    95.97(a)
    479656  XM_536788.2  XP_536788.2 
    chimpanzee
    (Pan troglodytes)
    AARS1   -- alanyl-tRNA synthetase 99.31(n)
    99.69(a)
    454210  XM_001169474.1  XP_001169474.1 
    cow
    (Bos taurus)
    AARS1   -- alanyl-tRNA synthetase 89.98(n)
    95.44(a)
    510933  XM_588170.3  XP_588170.3 
    rat
    (Rattus norvegicus)
    Aars1   -- alanyl-tRNA synthetase 88.05(n)
    95.14(a)
    292023  XM_214690.4  XP_214690.3 
    mouse
    (Mus musculus)
    Aars1, 5 85
    alanyl-tRNA synthetase1, 5 88.05(n)1
    95.56(a)1
    2347341  NM_146217.31  NP_666329.21 
     AI3164955  AK0444515  (see all 44)
    About this table        Species with no ortholog for AARS

    ENSEMBL Gene Tree for AARS
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for AARS gene
    AARS22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/184 NCBI SNPs in AARS are shown (see all 184 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 31)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 16 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs71988711,2
    A,F,H68882819(+) TTTCAG/AAATAA 1 -- ng314Minor allele frequency- A:0.08EU EA WA 416
    rs115376671,2
    C,F68861160(-) CACAGA/GTGCCC 1 G/D mis15Minor allele frequency- G:0.13NA EU EA WA 492
    rs99229691,2
    H68881717(+) ccaacA/Ggatga 1 -- ng314Minor allele frequency- G:0.00EU EA WA 414
    rs20702031,2
    A,C,F,H,O68861081(-) GACCAC/TGCTCG 1 H/H syn116Minor allele frequency- T:0.48EA EU WA NA 2212
    --
    rs80637491,2
    A,C,F,H68876538(+) tgagaT/Cgggag 1 -- int14Minor allele frequency- C:0.07EU EA WA 418
    rs71909211,2
    A,C,F,H68856902(+) GGTTGG/CAAGGT 1 -- int14Minor allele frequency- C:0.07EU EA WA 412
    rs71920001,2
    A,C,F,H68856921(+) TCCTGT/CTCACT 1 -- int14Minor allele frequency- C:0.09EU EA WA 412
    rs72059181,2
    A,C,F,H68846233(+) AGGCCT/CTGCAG 1 -- int18Minor allele frequency- C:0.08EU EA WA 832
    --
    rs80466621,2
    A,C,F,H68845725(+) TAAATA/GCCAGG 1 -- int14Minor allele frequency- G:0.07EU EA WA 412
    --
    rs80467861,2
    A,F,H68876985(+) gtaacC/Ttggat 1 -- int14Minor allele frequency- T:0.08EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for AARS (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601065

    2 Novoseek disease relationships for AARS gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    myositis 61.76 1 16085368 (1)
    myopathies idiopathic inflammatory 57.50 1 16085368 (1)
    About this table

    Human Gene Mutation Database: AARS

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/85 PubMed articles for AARS gene (see all 85 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 16 HGNC: 20 AceView: AARS Ensembl:ENSG00000090861 euGenes: HUgn16
    ECgene: AARS H-InvDB: AARS
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for AARS:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for AARS-related products

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     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for AARS Antibodies for AARS
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for AARS Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for AARS

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