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AADAT Gene

protein-coding   GIFtS: 53

GC04M171218
aminoadipate aminotransferase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AadAT 3
EC 2.6.1.7,EC 2.6.1.39 3
KAT/AadAT 3
KAT2 1, 2, 3, 5
KATII 1, 2
Descriptions
2-aminoadipate aminotransferase 3
2-aminoadipate transaminase 3
Alpha-aminoadipate aminotransferase 3
Kynurenine aminotransferase II 2, 3
Kynurenine--oxoglutarate aminotransferase II 3
Kynurenine--oxoglutarate transaminase II 3
L kynurenine/alpha aminoadipate aminotransferase 2
aminoadipate aminotransferase 2
External Ids
HGNC: 179291
Entrez Gene: 511662
UniProtKB: Q8N5Z03
Ensembl: ENSG000001095767
Search outside databases for aliases for AADAT gene

Previous GC identifers: GC00U991148 GC04M171565 GC04M171677 GC04M171356

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for AADAT:
This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II.
The rat protein is a homodimer with two transaminase activities. One activity is the
transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the
major pathway for L-lysine catabolism. The other activity involves the transamination of
kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective
properties. Two alternative transcripts encoding the same isoform have been identified, however,
additional alternative transcripts and isoforms may exist. [provided by RefSeq]

UniProtKB/Swiss-Prot: AADAT_HUMAN, Q8N5Z0
Function: Shows activity also towards tryptophan, aspartate and hydroxykinurenine

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AADAT gene  

Entrez Gene cytogenetic band: 4q33   Ensembl cytogenetic band:  4q33   HGNC cytogenetic band: 4q33

AADAT Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M171218:     (about GC identifiers)

Start:
171,217,948 bp from pter
End:
171,247,947 bp from pter
Size:
30,000 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000004.10  NT_022792.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: AADAT_HUMAN, Q8N5Z0 (See protein sequence)
Recommended Name: Kynurenine/alpha-aminoadipate aminotransferase mitochondrial precursor  
Size: 425 amino acids; 47352 Da
Cofactor: Pyridoxal phosphate
Subunit: Homodimer (By similarity)
Subcellular location: Mitochondrion (Potential)
PDB structures from and Proteopedia :
2QLR (3D)    2R2N (3D)    2VGZ (3D)    3DC1 (3D)    
Secondary accessions: Q9UL02
Alternative splicing: 2 isoforms:  Q8N5Z0-1   Q8N5Z0-2   

REFSEQ proteins (2 alternative transcripts): 
NP_057312.1  NP_872603.1  


ENSEMBL proteins: 
ENSP00000336808 ENSP00000226840 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Aminoadipate aminotransferase)
Human Recombinant Proteins from Abnova (AADAT)
              OriGene Purified Recombinant Human Protein: AADAT 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005739 mitochondrion IEA--
GO:0005829 cytosol EXP12126930
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Antibodies for AADAT: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for AADAT
Browse R&D Systems for Antibodies
Antibodies from Abcam (Aminoadipate aminotransferase), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (AADAT)
Novus Biologicals Antibodies for AADAT

Assays for AADAT: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR004839 Aminotrans_I/II
 IPR015421 PyrdxlP-dep_Trfase_major_sub1


   GeneDecks  AADAT for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q8N5Z0

ProtoNet protein and cluster: Q8N5Z0

UniProtKB/Swiss-Prot: AADAT_HUMAN, Q8N5Z0
Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (AADAT)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (AADAT)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_182662

              Applied Biosystems Silencer® siRNAs for AADAT

              Sigma-Aldrich siRNA for AADAT  
                     Sigma-Aldrich shRNA for AADAT  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for AADAT
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_016228
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_016228
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_016228 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_182662

UniProtKB/Swiss-Prot: AADAT_HUMAN, Q8N5Z0
Function: Shows activity also towards tryptophan, aspartate and hydroxykinurenine
Catalytic activity: L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate +
L-glutamate
Catalytic activity: L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate
Enzyme Number (IUBMB): EC 2.6.1.7,EC 

3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Aadat):

behavior/neurologicalnervous systemvision/eye

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016212 kynurenine-oxoglutarate transaminase activity IEA--
GO:0030170 pyridoxal phosphate binding IEA--
GO:0047536 2-aminoadipate transaminase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for AADAT (Maps provided by GeneGo):
 Leucine and lysine metabolism

   GeneDecks  AADAT for the pathways selected above  
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3 Sigma-Aldrich "Your Favorite Gene" Pathways for  AADAT  (Your Favorite Gene powered by Ingenuity) 
 Tryptophan Metabolism
 Lysine Biosynthesis
 Lysine Degradation

   GeneDecks  AADAT for the pathways selected above  
About GeneDecksing

3 Kegg Pathways  (Kegg details for AADAT):
 hsa00300 Lysine biosynthesis
 hsa00310 Lysine degradation
 hsa00380 Tryptophan metabolism

   GeneDecks  AADAT for the pathways selected above  
About GeneDecksing
UniProtKB/Swiss-Prot: AADAT_HUMAN, Q8N5Z0
Pathway: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from
L-lysine: step 4/6


5/11 Interacting proteins for AADAT (ENSP000003368083) via UniProtKB, MINT, and/or STRING (see all 11 )

InteractantInteraction Details
GeneCardExternal ID(s)
KMOENSP000003555133STRING (score=.966)
KYNUENSP000002641703STRING (score=.904)
AASDHPPTENSP000002786183STRING (score=.899)
AFMIDENSP000003289383STRING (score=.899)
AKR1B10ENSP000003525843STRING (score=.899)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0009058 biosynthetic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for AADAT
1 Novoseek chemical compound relationship for AADAT gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
kynurenine 93.14 23 8350986 (2), 10441733 (2), 18056995 (2), 16511030 (2) (see all 13)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (AADAT)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (AADAT)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_182662

              Sigma-Aldrich siRNA for AADAT  
                     Sigma-Aldrich shRNA for AADAT  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_016228  NM_182662  

REFSEQ mRNAs for AADAT gene (2 alternative transcripts): 

NM_016228.3   NM_182662.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_016228  NM_182662  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_016228
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_016228
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_016228 

Additional cDNA sequence: 

AF481738.1 AK055952.1 AK314729.1 BC031068.1 

10 DOTS entries:

DT.310471  DT.100019700  DT.40299440  DT.95259621  DT.91865689  DT.95245901  DT.100719445  DT.108019 
DT.87046874  DT.95340712 

24/80 AceView cDNA sequences (see all 80 ):

F08882 CB215768 BU154412 CD722938 AI819354 H92545 NM_182662 AI393635 
BQ287909 W05694 BC031068 AA568464 N54512 AI024796 BX281257 H72204 
AK055952 F11219 AW472959 AI580846 NM_016228 CA436887 BU741977 BI597437 

highest scoring ESTs for AADAT:

AI819354 AK055952 BC031068 BM478854 AA059063 AA121140 AA333897 AA401238 AA404282 AA434578 

Unigene Cluster for AADAT:

Aminoadipate aminotransferase
Hs.529735  [show with all ESTs]
Unigene Representative Sequence: AF481738


GeneLoc Exon Structure

5/8 Alternative Splicing Database (ASD) splice patterns (SP) for AADAT (see all 8 )

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10a · 10b · 10c · 10d ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^
SP1:                                            -           -     -                                                                 -                           
SP2:                                            -                 -                                                                 -                           
SP3:                                                        -     -                                                                 -                           
SP4:              -     -     -     -     -     -           -     -                                                                                             
SP5:                                      -     -           -     -                                                                                             

ExUns: 16 ^ 17 ^ 18a · 18b · 18c
SP1:                              
SP2:                              
SP3:                              
SP4:                              
SP5:                              

About this scheme

ECgene alternative splicing isoforms for AADAT

2 Ensembl transcripts including schematic representations:
ENST00000337664  ENST00000353187  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

AADAT expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for AADAT

1 / 2 / 3

3 probe-sets matching AADAT gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
61370_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AI819354 0.80 1.00 0.91 1

223593_at2, 3 U133-B 2 1.00 0.95 -- -- AF097994 0.40 1.00 0.76 1

223593_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
GeneDecks  AADAT for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TCCTATCTGT

SOURCE GeneReport for Unigene cluster: Hs.529735

UniProtKB/Swiss-Prot: AADAT_HUMAN, Q8N5Z0
Tissue specificity: Higher expression in the liver. Also found in heart, brain, kidney, pancreas,
prostate, testis and ovary

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for AADAT gene from 5/12 species (see all 12 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
AADAT1   -- aminoadipate aminotransferase 86.97(n)
82.77(a)
486059  XM_543185.2  XP_543185.2 
chimpanzee
(Pan troglodytes)
AADAT1   -- aminoadipate aminotransferase 99.29(n)
99.06(a)
461601  XM_001154960.1  XP_001154960.1 
cow
(Bos taurus)
AADAT1   -- aminoadipate aminotransferase 85.25(n)
80.24(a)
508929  NM_001015551.1  NP_001015551.1 
rat
(Rattus norvegicus)
Aadat1   -- aminoadipate aminotransferase 77.23(n)
72.1(a)
29416  NM_017193.1  NP_058889.1 
mouse
(Mus musculus)
Aadat1, 5 8 (31.50 cM)5
aminoadipate aminotransferase1, 5 79.14(n)1
73.41(a)1
239231  NM_011834.21  NP_035964.11 
 AA0735415  AA1058235  (see all 13)
About this table        Species with no ortholog for AADAT

ENSEMBL Gene Tree for AADAT
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/122 NCBI SNPs in AADAT are shown (see all 122 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 44)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 4 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs29552551,2
A,C171248954(+) gtgtgG/Taggga 2 -- ng310--------
rs29288191,2
A,C171248515(+) TGAGGA/GAAGGG 2 -- ng31 trp30--------
--
rs29288201,2
C171249197(+) cggggC/Tgggac 2 -- ng310--------
--
rs46927911,2
C171249316(+) cccatC/Ttaccg 2 -- ng310--------
--
rs46927901,2
C171249283(+) acgagC/Tgccgc 2 -- ng310--------
rs126406521,2
C,F,H171230925(+) TTTGGA/GACAGT 2 V/V syn1 ese34Minor allele frequency- G:0.01EU EA WA 390
rs24669901,2
A,C,F,H,O171230346(+) CAAAAG/AGCACA 2 -- int18Minor allele frequency- A:0.26EU EA WA 828
rs7168221,2
A,C,F,H,O171244079(-) GTTCCG/AGGAAC 2 -- int110Minor allele frequency- A:0.07NA EA MN EU WA 1022
rs14805441,2
A,C,F,H,O171224211(-) TTCTTC/TTTCTA 2 -- int1 trp37Minor allele frequency- T:0.33MN NA EU EA WA 784
rs15859041,2
A,C,F,H171241072(+) ggaaaC/Tgcaat 2 -- int14Minor allele frequency- T:0.05EU EA WA 398
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HapMap Linkage Disequilibrium images for AADAT (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611754

1 Novoseek disease relationship for AADAT gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
huntington disease 43.23 1 10441733 (1)
About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/21 PubMed articles for AADAT gene (see all 21 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 51166 HGNC: 17929 AceView: AADAT Ensembl:ENSG00000109576 euGenes: HUgn51166
ECgene: AADAT H-InvDB: AADAT
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for AADAT:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for AADAT Antibodies for AADAT
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for AADAT Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for AADAT




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for AADAT

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