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AADACL1 Gene

protein-coding   GIFtS: 47

GC03M173832
arylacetamide deacetylase-like 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 3.1.1.- 3
KIAA1363 1, 3
NCEH 2, 3
Descriptions
Neutral cholesterol ester hydrolase 2, 3
arylacetamide deacetylase-like 1 2
External Ids
HGNC: 292601
Entrez Gene: 575522
UniProtKB: Q6PIU23
Ensembl: ENSG000001449597
Search outside databases for aliases for AADACL1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ADCL1_HUMAN, Q6PIU2
Function: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for
de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester
hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also
involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May
contribute to cancer pathogenesis by promoting tumor cell migration

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AADACL1 gene  

Entrez Gene cytogenetic band: 3q26.31   Ensembl cytogenetic band:  3q26.31   HGNC cytogenetic band: 3q26.31

AADACL1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M173832:     (about GC identifiers)

Start:
173,831,129 bp from pter
End:
173,911,702 bp from pter
Size:
80,574 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ADCL1_HUMAN, Q6PIU2 (See protein sequence)
Recommended Name: Arylacetamide deacetylase-like 1  
Size: 408 amino acids; 45808 Da
Subcellular location: Membrane; Single-pass type II membrane protein (Probable). Microsome (By
similarity)
Secondary accessions: B7Z2K4 B7Z3A1 B7Z5U2 B7Z906 B7ZAW6 Q86WZ1 Q9P2I4
Alternative splicing: 3 isoforms:  Q6PIU2-1   Q6PIU2-2   Q6PIU2-3   

Post-translational modifications:

  • N-glycosylated1


  • REFSEQ proteins (4 alternative transcripts): 
    NP_001139748.1  NP_001139749.1  NP_001139750.1  NP_065843.3  


    ENSEMBL proteins: 
    ENSP00000273512 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (AADACL1)
    Human Recombinant Proteins from Abnova (AADACL1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005792 microsome IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for AADACL1: 
    Browse Antibodies Central at Invitrogen
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    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (AADACL1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (AADACL1)
    Novus Biologicals Antibody for AADACL1

    Assays for AADACL1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR017157 Arylacetamide_deacetylase
     IPR013094 AB_hydrolase_3
     IPR002168 Lipase_GDXG_AS


       GeneDecks  AADACL1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q6PIU2

    ProtoNet protein and cluster: Q6PIU2

    2 Blocks protein families:
    IPB002168 Lipolytic enzyme
    IPB013094 Alpha/beta hydrolase fold-3


    UniProtKB/Swiss-Prot: ADCL1_HUMAN, Q6PIU2
    Similarity: Belongs to the 'GDXG' lipolytic enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AADACL1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020792

                  Applied Biosystems Silencer® siRNAs for AADACL1

                  Sigma-Aldrich siRNA and siRNA Panels for AADACL1  
                         Sigma-Aldrich shRNA for AADACL1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020792
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020792
                                     untagged cDNA clone in CMV expression vector: NM_020792 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_020792

    UniProtKB/Swiss-Prot: ADCL1_HUMAN, Q6PIU2
    Function: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for
    de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester
    hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also
    involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May
    contribute to cancer pathogenesis by promoting tumor cell migration
    Enzyme Number (IUBMB): EC 3.1.1.- 

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Aadacl1):

    homeostasis/metabolism

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004091 carboxylesterase activity IEA--
    GO:0016787 hydrolase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008152 metabolic process IEA--
    GO:0016042 lipid catabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for AADACL1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AADACL1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020792

                  Sigma-Aldrich siRNA and siRNA Panels for AADACL1  
                         Sigma-Aldrich shRNA for AADACL1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_020792  

    REFSEQ mRNAs for AADACL1 gene (4 alternative transcripts): 

    NM_001146276.1   NM_001146277.1   NM_001146278.1   NM_020792.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_020792  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020792
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020792
                                     untagged cDNA clone in CMV expression vector: NM_020792 

    Additional cDNA sequence: 

    AB037784.1 AK294811.1 AK295641.1 AK299422.1 AK304253.1 AK316431.1 AL512752.1 BC028734.1 
    BC047588.1 

    9 DOTS entries:

    DT.446475  DT.100774528  DT.95302321  DT.97777363  DT.100774526  DT.100774527  DT.120896588  DT.120896590 
    DT.120896610 

    24/97 AceView cDNA sequences (see all 97 ):

    BQ009207 AI859996 AI052302 BM968147 AA625233 AI419800 BX281107 BE218940 
    BX378214 AI192253 AW192003 AW188964 AI962960 AA971330 BM128997 NM_020792 
    CA423166 BG778641 AI948947 BF435737 AA548653 BE877591 BM712744 CB138758 

    highest scoring ESTs for AADACL1:

    AB037784 AI769531 AI948947 AL119157 BC028734 BC047588 BG701753 BI666603 BM922259 U46357 

    Unigene Cluster for AADACL1:

    Arylacetamide deacetylase-like 1
    Hs.444099  [show with all ESTs]
    Unigene Representative Sequence: BC028734


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000273512  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    AADACL1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for AADACL1

    1 / 2 / 3

    5 probe-sets matching AADACL1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    43038_r_at2, 3 U95-B 1 1.00 1.00 0.89 1.37 AI769531 0.80 1.00 0.91 1
    77022_at2, 3 U95-E 1 1.00 1.00 0.94 1.21 AL119157 0.80 1.00 0.91 1

    76435_at2, 3 U95-E 2 1.00 0.97 0.88 0.45 AI948947 0.80 1.00 0.91 1

    225847_at2, 3 U133-B 1 1.00 1.00 -- -- AB037784 0.80 1.00 0.91 1

    225847_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  AADACL1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TTTGGAAATA

    SOURCE GeneReport for Unigene cluster: Hs.444099

    UniProtKB/Swiss-Prot: ADCL1_HUMAN, Q6PIU2
    Tissue specificity: Expressed in monocyte-derived macrophages. Up-regulated in invasive melanoma
    and breast carcinoma cell lines

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for AADACL1 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    AADACL11   -- arylacetamide deacetylase-like 1 87.32(n)
    92.68(a)
    488171  XM_545295.2  XP_545295.2 
    chimpanzee
    (Pan troglodytes)
    AADACL11   -- arylacetamide deacetylase-like 1 99.02(n)
    98.64(a)
    470998  XM_526382.2  XP_526382.2 
    cow
    (Bos taurus)
    AADACL11   -- arylacetamide deacetylase-like 1 89.62(n)
    92.89(a)
    534212  XM_584246.3  XP_584246.3 
    mouse
    (Mus musculus)
    Aadacl11, 5 35
    arylacetamide deacetylase-like 11, 5 82.76(n)1
    87.5(a)1
    3200241  NM_178772.31  NP_848887.11 
     AI4264705  AI4507925  (see all 20)
    chicken
    (Gallus gallus)
    AADACL11   -- arylacetamide deacetylase-like 1 70.47(n)
    67.7(a)
    429158  XM_426713.2  XP_426713.2 
    About this table        Species with no ortholog for AADACL1

    ENSEMBL Gene Tree for AADACL1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for AADACL1 gene
    AADACL42  AADACL32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/682 NCBI SNPs in AADACL1 are shown (see all 682 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 144)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs126368971,2
    A,C,F,H173912566(+) GAGTAC/GTGAAT 1 -- ng316Minor allele frequency- G:0.47EU EA WA NA 600
    --
    rs37330231,2
    A,C,F,H173833700(+) GGTATC/GAGTAT 1 -- ut3116Minor allele frequency- N:0.00EU EA WA NA 1468
    rs22768051,2
    A,C,F,H173911476(+) TGCCCC/TGGCTC 1 Q/R mis112Minor allele frequency- T:0.37EA NA EU WA 2102
    rs44903881,2
    A,C,F,H173832829(+) TTTTTA/TAAAAA 1 -- ut317Minor allele frequency- T:0.24EU EA WA NA 514
    rs32051441,2
    C,F,H,O173831910(-) TACACA/GGACAC 1 -- ut31 ese317Minor allele frequency- N:0.00MN EA NA EU WA 1600
    rs23028151,2
    C,F,H173848526(+) TTTTTT/GGCCAA 1 Q/K mis113Minor allele frequency- G:0.15EA NA EU WA 2528
    rs353164201,2
    C,F173911414(-) ATTACG/TTCTAC 1 V/F mis15Minor allele frequency- T:0.01NA EU EA WA 482
    rs168459471,2
    F,H173832189(+) GCTACT/CGGCTC 1 -- ut31 ese37Minor allele frequency- C:0.05NA EU EA WA 562
    --
    rs74324591,2
    A,C173833034(+) ATTTTG/TAACTA 1 -- ut310--------
    rs108048481,2
    C173912813(+) GCTTCC/TTCTTC 1 -- ng31 trp30--------
    About this table

    HapMap Linkage Disequilibrium images for AADACL1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10 PubMed articles for AADACL1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 57552 HGNC: 29260 AceView: KIAA1363 Ensembl:ENSG00000144959 euGenes: HUgn57552
    ECgene: AADACL1 H-InvDB: AADACL1
    (According to HUGE)
    About This Section

    HUGE: KIAA1363
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for AADACL1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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