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AADAC Gene

protein-coding   GIFtS: 60

GC03P153014
arylacetamide deacetylase (esterase)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CES5A1 1, 2
DAC 1, 2, 3, 5
EC 3.1.1.- 3
Descriptions
arylacetamide deacetylase 2
arylacetamide deacetylase (esterase) 2
External Ids
HGNC: 171
Entrez Gene: 132
UniProtKB: P227603
Ensembl: ENSG000001147717
Search outside databases for aliases for AADAC gene

Previous GC identifers: GC03P148473 GC03P152364 GC03P152813 GC03P152852

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for AADAC:
Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine
N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine
and heterocyclic amine carcinogens [provided by RefSeq]

UniProtKB/Swiss-Prot: AAAD_HUMAN, P22760
Function: Arylacetamide deacetylation is an important enzyme activity in the metabolic activation
of arylamine substrates to ultimate carcinogens

Gene Wiki entry for AADAC

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AADAC gene  

Entrez Gene cytogenetic band: 3q21.3-q25.2   Ensembl cytogenetic band:  3q25.1   HGNC cytogenetic band: 3q21.3-q25.2

AADAC Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P153014:     (about GC identifiers)

Start:
153,014,551 bp from pter
End:
153,028,966 bp from pter
Size:
14,416 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: AAAD_HUMAN, P22760 (See protein sequence)
Recommended Name: Arylacetamide deacetylase  
Size: 399 amino acids; 45748 Da
Subcellular location: Endoplasmic reticulum membrane; Single-pass type II membrane protein (By
similarity). Microsome membrane; Single-pass type II membrane protein (By similarity)
Secondary accessions: Q8N1A9

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_001077.2  

    ENSEMBL proteins: 
    ENSP00000232892 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (AADAC)
    Human Recombinant Proteins from Abnova (AADAC)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IDA18029348
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005789 endoplasmic reticulum membrane IDA10318829 15152005
    GO:0005792 microsome TAS8063807
    GO:0016020 membrane IEA--
    About this table

    Antibodies for AADAC: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for AADAC
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (AADAC), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (AADAC)
    Novus Biologicals Antibodies for AADAC

    Assays for AADAC: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR017157 Arylacetamide_deacetylase
     IPR013094 AB_hydrolase_3
     IPR002168 Lipase_GDXG_AS


       GeneDecks  AADAC for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P22760

    ProtoNet protein and cluster: P22760

    2 Blocks protein families:
    IPB002168 Lipolytic enzyme
    IPB013094 Alpha/beta hydrolase fold-3


    UniProtKB/Swiss-Prot: AAAD_HUMAN, P22760
    Similarity: Belongs to the 'GDXG' lipolytic enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AADAC)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (AADAC)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001086

                  Applied Biosystems Silencer® siRNAs for AADAC

                  Sigma-Aldrich siRNA and siRNA Panels for AADAC  
                         Sigma-Aldrich shRNA for AADAC  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for AADAC
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001086
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001086
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001086 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001086

    UniProtKB/Swiss-Prot: AAAD_HUMAN, P22760
    Function: Arylacetamide deacetylation is an important enzyme activity in the metabolic activation
    of arylamine substrates to ultimate carcinogens
    Enzyme Number (IUBMB): EC 3.1.1.- 

    Genatlas biochemistry entry for AADAC:
    arylacetamide deacetylase,microsomal,liver

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004091 carboxylesterase activity IEA--
    GO:0016298 lipase activity TAS11481320
    GO:0019213 deacetylase activity IDA9665742
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  AADAC  (Your Favorite Gene powered by Ingenuity) 
     Butanoate Metabolism

       GeneDecks  AADAC for the pathways selected above  
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    1 Kegg Pathway  (Kegg details for AADAC):
     hsa00960 Alkaloid biosynthesis II

       GeneDecks  AADAC for the pathways selected above  
    About GeneDecksing

    5/7 Interacting proteins for AADAC (ENSP000002328923) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CES1ENSP000003537203STRING (score=.927)
    CES7ENSP000002905673STRING (score=.909)
    LIPAENSP000003608953STRING (score=.899)
    DDHD1ENSP000003271043STRING (score=.8)
    ENSG00000214517ENSP000003298673STRING (score=.8)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008152 metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for AADAC
    1 Novoseek chemical compound relationship for AADAC gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    arylamine 78.22 3 8063807 (2)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (AADAC)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (AADAC)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001086

                  Sigma-Aldrich siRNA and siRNA Panels for AADAC  
                         Sigma-Aldrich shRNA for AADAC  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001086  

    REFSEQ mRNAs for AADAC gene: 

    NM_001086.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001086  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001086
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001086
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001086 

    Additional cDNA sequence: 

    AK290628.1 BC020706.1 BC032309.1 CR624107.1 L32179.1 

    6 DOTS entries:

    DT.415010  DT.95156022  DT.101980759  DT.92460876  DT.99957497  DT.434029 

    24/68 AceView cDNA sequences (see all 68 ):

    AV685871 CR624107 CB156860 CB148233 BC032309 CB105350 BG542396 AA045221 
    AA702700 CF597071 AW951425 BI760687 AV702720 AA777647 N76857 AA045320 
    BQ695358 L32179 AV705031 AV703616 AA700863 BX431750 BX443932 BG567565 

    highest scoring ESTs for AADAC:

    L32179 AA045221 AA045320 AA700863 AA702448 AA702700 AA777647 AI032048 AI247922 AI274232 

    Unigene Cluster for AADAC:

    Arylacetamide deacetylase (esterase)
    Hs.506908  [show with all ESTs]
    Unigene Representative Sequence: NM_001086


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000232892  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    AADAC expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for AADAC

    1 / 2 / 3

    3 probe-sets matching AADAC gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    36512_at2, 3 U95-A 2 1.00 0.97 1.00 1.00 L32179 1.00 1.00 1.00 1

    205969_at2, 3 U133-A 2 1.00 0.95 -- -- NM_001086 0.60 1.00 0.82 1

    205969_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
    GeneDecks  AADAC for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TAGCATAATT

    SOURCE GeneReport for Unigene cluster: Hs.506908

    Expression variation in blood from EXPOLDB for AADAC

    UniProtKB/Swiss-Prot: AAAD_HUMAN, P22760
    Tissue specificity: Liver

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for AADAC gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    AADAC1   -- arylacetamide deacetylase (esterase) 81.12(n)
    75.44(a)
    477115  XM_534309.2  XP_534309.2 
    chimpanzee
    (Pan troglodytes)
    AADAC1   -- arylacetamide deacetylase (esterase) 99.5(n)
    99.25(a)
    460785  XM_001145851.1  XP_001145851.1 
    cow
    (Bos taurus)
    AADAC1   -- arylacetamide deacetylase (esterase) 76.94(n)
    71.18(a)
    519557  NM_001075791.1  NP_001069259.1 
    rat
    (Rattus norvegicus)
    Aadac1   -- arylacetamide deacetylase (esterase) 73.87(n)
    68.09(a)
    57300  NM_020538.1  NP_065413.1 
    mouse
    (Mus musculus)
    Aadac1, 5 35
    arylacetamide deacetylase (esterase)1, 5 72.73(n)1
    69.95(a)1
    677581  NM_023383.11  NP_075872.11 
     AF3067885  AI0475085  (see all 10)
    About this table        Species with no ortholog for AADAC

    ENSEMBL Gene Tree for AADAC
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for AADAC gene
    AADACL22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/151 NCBI SNPs in AADAC are shown (see all 151 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 66)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs21209821,2
    A,C,F,H,O153012811(-) TGTTGA/GTTTTT 1 -- ng51 tfbs316Minor allele frequency- N:0.00EU EA WA NA 1472
    rs18031551,2
    A,C,F,H153028291(+) AATTTA/GTTAAT 1 V/I mis112Minor allele frequency- G:0.37MN EU EA WA 2694
    --
    rs22930051,2
    A,C,F,H153014049(-) AAGTGT/CTCCAC 1 -- ng514Minor allele frequency- C:0.18EU EA WA 400
    rs10422011,2
    C,F,H,O153028731(+) TCTTAG/AAATGG 1 -- ut3110Minor allele frequency- A:0.43EA MN EU WA 2502
    --
    rs168339351,2
    C,F,H153013003(+) CAATTC/TGACTC 1 -- ng5116Minor allele frequency- T:0.21NA EU EA WA 1472
    rs22930041,2
    C,F,H153014584(-) GATATG/ACCTGA 1 -- ut5117Minor allele frequency- A:0.33EA NA EU WA 2926
    --
    rs21395481,2
    A,C153013134(+) ttgcaG/Tgaatc 1 -- ng510--------
    rs355444171,2
    F153014679(-) ATGAGG/TATCCC 1 I/I syn11Minor allele frequency- T:0.03NA 78
    rs22719421,2
    A,C,F,H,O153018112(-) AATGTC/TTGCGA 1 -- int122Minor allele frequency- N:0.00NS EA NA EU WA 2976
    rs18509641,2
    A,C,F,H,O153019533(-) CTTAAT/CTTCAA 1 -- int18Minor allele frequency- C:0.16MN NA EU EA WA 724
    About this table

    HapMap Linkage Disequilibrium images for AADAC (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 600338

    Human Genome Epidemiology Navigator: AADAC (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for AADAC gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 13 HGNC: 17 AceView: AADAC Ensembl:ENSG00000114771 euGenes: HUgn13
    ECgene: AADAC H-InvDB: AADAC
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for AADAC Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for AADAC:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for AADAC Antibodies for AADAC
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
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     Antibodies for AADAC

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