A4GNT Gene
protein-coding GIFtS : 50
GCID: GC03 M137842
alpha-1,4-N-acetylglucosaminyltransferase
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Aliasesfor A4GNT gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases Alpha-1,4-N-Acetylglucosaminyltransferase 1 Alpha4GnT3 Alpha4GnT3 EC 2.4.1.-3
Export aliases for A4GNT gene to outside databases Previous GC identifers: GC03M139325 GC03M135217
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Summariesfor A4GNT gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section Entrez Gene summary for A4GNT : This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. (provided by RefSeq, Jul 2008) UniProtKB/Swiss-Prot: A4GCT_HUMAN, Q9UNA3 Function : Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core2 branched O-glycans
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Genomic Viewsfor A4GNT gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000003.11 NC_018914.1 NT_005612.16 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the A4GNT gene promoter: Bach1 E2F-3a E2F-4 E2F-5 E2F-2 E2F-1 E2F POU2F1 POU2F1a FOXO1a Other transcription factors Search SABiosciences Chromatin IP Primers for A4GNT Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat A4GNT
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 3p14.3 Ensembl cytogenetic band: 3q22.3 HGNC cytogenetic band: 3p14.3 A4GNT Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc information about chromosome 3 GeneLoc Exon Structure
GeneLoc location for GC03M137842: view genomic region
(about GC identifiers )
Start:
137,842,560 bp from pter
End:
137,851,229 bp from pter
Size:
8,670 bases
Orientation:
minus strand
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Proteinsfor A4GNT gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: A4GCT_HUMAN, Q9UNA3 (See
protein sequence )Recommended Name: Alpha-1,4-N-acetylglucosaminyltransferase Size : 340 amino acids; 39497 Da
Subcellular location : Golgi apparatus membrane; Single-pass type II membrane protein (Probable)
Secondary accessions : Q0VDK1 Q0VDK2Explore the universe of human proteins at neXtProt for A4GNT: NX_Q9UNA3 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_Q9UNA3 A4GNT Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins: NP_057245.1 ENSEMBL proteins: ENSP00000236709 Human Recombinant Protein Products: Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view) : About this table
A4GNT for ontologies About GeneDecksing A4GNT Antibody Products: Assay Products for A4GNT:
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Protein
Domains / Familiesfor A4GNT gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section
A4GNT for domains About GeneDecksing 2 InterPro domains/families :
Graphical View of Domain Structure for InterPro Entry Q9UNA3 ProtoNet protein and cluster: Q9UNA3
1 Blocks protein family : IPB007652 Alpha 1,4-glycosyltransferase conserved region UniProtKB/Swiss-Prot: A4GCT_HUMAN, Q9UNA3 Domain : The conserved DXD motif is involved in enzyme activity (By similarity)Similarity : Belongs to the glycosyltransferase 32 family
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Functionfor A4GNT gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: A4GCT_HUMAN, Q9UNA3 Function : Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core2 branched O-glycans Enzyme Number (IUBMB): EC 2.4.1.- 1
Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for A4GNT (see all 2 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for A4GNTOriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: A4GNT (NM_016161 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for A4GNT Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat A4GNT
In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for A4GNT
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view) : About this table
GO ID Qualified GO term Evidence PubMed IDs GO:0008375 acetylglucosaminyltransferase activity
IDA 10430883 GO:0008378 galactosyltransferase activity
IEA --
A4GNT for ontologies About GeneDecksing 1 GenomeRNAi human phenotype for A4GNT :Animal Models: Mouse knock-out A4gnt tm1Jnaka for A4GNT 5 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for A4gnt) :
A4GNT for phenotypes About GeneDecksing
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Pathways & Interactionsfor A4GNT gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Unified GeneCards pathways   About this table See pathways by source Super-pathway contained gene-specific pathways 1 heparan sulfate biosynthesis
2 BioSystems Pathways for A4GNT UniProtKB/Swiss-Prot: A4GCT_HUMAN, Q9UNA3 Pathway : Protein modification; protein glycosylationInteractions: Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for A4GNT
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view) : About this table
A4GNT for ontologies About GeneDecksing
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Drugs & Compoundsfor A4GNT gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Browse Tocris compounds for A4GNT Search CenterWatch for drugs/clinical trials and news about A4GNT / A4GCT
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Transcriptsfor A4GNT gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for A4GNT gene: NM_016161.2 Unigene Cluster for A4GNT:
Alpha-1,4-N-acetylglucosaminyltransferase Hs.278960 [show with all ESTs ] Unigene Representative Sequence: AK225668 1 Ensembl transcript including schematic representation, and UCSC links where relevant : ENST00000236709 (uc003ers.2 ) Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for A4GNT (see all 2 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for A4GNTOriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: A4GNT (NM_016161 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for A4GNT Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat A4GNT
Additional cDNA sequence: AF141315.1 AK225668.1 BC119639.1 BC119640.2
1 DOTS entry : DT.91743826
7 AceView cDNA sequences :
AF141315 NM_016161 BM769023 BX094798 BP343384 AI344512 AW869791 GeneLoc Exon Structure
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Expression for A4GNT gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section A4GNT expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: AACCAGGAGG
About this image A4GNT expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table See A4GNT Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for A4GNT SOURCE GeneReport for Unigene cluster: Hs.278960 UniProtKB/Swiss-Prot: A4GCT_HUMAN, Q9UNA3 Tissue specificity : Detected in stomach and pancreas SABiosciences Expression via Pathway-Focused PCR Array including A4GNT : Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for A4GNTBrowse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat A4GNT QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat A4GNT QIAGEN QuantiFast Probe-based Assays in human , mouse , rat A4GNT In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for A4GNT
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Orthologsfor A4GNT gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
This gene was present in the common ancestor of eukaryotes.
Orthologs for A4GNT gene from 7/14 species (see all 14 ) About this table
ENSEMBL Gene Tree for A4GNT (if available)TreeFam Gene Tree for A4GNT (if available)
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Paralogsfor A4GNT gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for A4GNT gene A4GALT 2 1 SIMAP similar gene for A4GNT using alignment to 1 protein entry: A4GCT_HUMAN :A4GALT
A4GNT for paralogs About GeneDecksing 1 Pseudogenes.org Pseudogene for A4GNT PGOHUM00000237787
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Genomic Variantsfor A4GNT gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section
Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical significance Chr 3 pos Sequence # AA Chg Type More # Allele freq Pop Total sample More
HapMap Linkage Disequilibrium report for A4GNT (137842560 - 137851229 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 2 variations for A4GNT 2 CNVs : 8991 3449 Human Gene Mutation Database (HGMD) : A4GNT SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing A4GNT
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Disorders
/ Diseasesfor A4GNT gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section
A4GNT for disorders About GeneDecksing 3 diseases for A4GNT : About MalaCards gastric cancer pancreatic cancer pancreatitis 1 disease from the University of Copenhagen DISEASES database for A4GNT :Gastric adenocarcinoma Human Genome Epidemiology (HuGE) Navigator: A4GNT (2 documents) Export disorders for A4GNT gene to outside databases
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Publicationsfor A4GNT gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for A4GNT gene, integrated from 9 sources (see all 13 ): (articles sorted by number of sources associating them with A4GNT) Utopia : connect your pdf to the dynamic world of online information
Expression cloning of a human alpha1, 4-N- acetylglucosaminyltransferase that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the gastric gland mucous cell-type mucin. (PubMed id 10430883) 1 , 2 , 3, 9 Nakayama J.... Fukuda M. (1999) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 1 , 2 Gerhard D.S....Malek J. (2004) Immunohistochemical demonstration of alpha1,4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1,4Galbeta residues in human gastrointestinal mucosa. (PubMed id 11304796) 1 , 9 Zhang M.X....Fukuda M. (2001) Essential role of gastric gland mucin in preventing ga stric cancer in mice. (PubMed id 22307328) 1 Karasawa F....Nakayama J. (2012) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614) 1 Rose J.E....Uhl G.R. (2010) Genetic variation in a4GnT in relation to Helicobacte r pylori serology and gastric cancer risk. (PubMed id 19751437) 1 Zheng Z....Ye W. (2009) Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348) 1 Barbe L....Andersson-Svahn H. (2008) Clinical utility of quantitative RT-PCR targeted to alpha1,4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer. (PubMed id 16441422) 1 Ishizone S....Nakayama J. (2006) Usefulness of the real-time reverse transcription-polymerase chain reaction assay targeted to alpha1,4-N-acetylglucosaminyltransferase for the detection of gastric cancer. (PubMed id 12594234) 1 Shimizu F....Katsuyama T. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 1 Strausberg R.L....Marra M.A. (2002)
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External Searches for A4GNT gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing A4GNT gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing A4GNT gene
(According to HUGE )
About This Section --
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Specialized Databases showing A4GNT gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
Name Description
PharmGKB entry for A4GNT Pharmacogenomics, SNPs, Pathways GGDB http://riodb.ibase.aist.go.jp/rcmg/ggdb/ Functional Glycomics Gateway - GTase http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_532
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About This Section Patent Information for A4GNT gene: Search GeneIP for patents involving A4GNT GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor A4GNT gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
OriGene Antibodies for A4GNT OriGene shRNA RFP for A4GNT OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for A4GNT OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for A4GNT OriGene Protein Over-expression Lysate for A4GNT Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for A4GNT Browse 3'-UTR reporter clones for miRNA target validation OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for A4GNT OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for A4GNT Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for A4GNT OriGene Custom Protein Services for A4GNT OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat A4GNT QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing A4GNT QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat A4GNT QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat A4GNT QIAGEN QuantiFast Probe-based Assays in human , mouse , rat A4GNT QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat A4GNT
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for A4GNT
ThermoFisher Antibodies for A4GNT
Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat A4GNT
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