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Aliases & Descriptions for A1CF
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases ACF 1 , 2 , 3 ACF64 1 , 2 ACF65 1 , 2 APOBEC1CF 1 , 2 ASP 1 , 2 , 3 MGC163391 2 OTTHUMP00000019611 2 OTTHUMP00000019612 2 OTTHUMP00000019614 2 OTTHUMP00000019615 2 OTTHUMP00000061209 2 RP11-564C4.2 2
Descriptions APOBEC-1 stimulating protein 2 APOBEC1 complementation factor 2 APOBEC1-stimulating protein 3 apo-B RNA editing protein 2 apobec-1 complementation factor 2 apobec-1 complementation factor (ACF) (ASP) 2
Search outside databases for aliases for A1CF gene
Summaries for A1CF (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for A1CF : Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by amulti-component enzyme complex containing a minimal core composed of APOBEC-1 and acomplementation factor encoded by this gene. The gene product has three non-identical RNArecognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposedthat this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 todeaminate the upstream cytidine. Studies suggest that the protein may also be involved in otherRNA editing or RNA processing events. Alternative splicing occurs at this locus and threefull-length transcript variants, encoding three distinct isoforms, have been described. Additionalsplicing has been observed but the full-length nature of these variants has not been determined.[provided by RefSeq] UniProtKB/Swiss-Prot: A1CF_HUMAN, Q9NQ94 Function : Essential component of the apolipoprotein B mRNA editing enzyme complex which isresponsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop inAPOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit,APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects theedited APOB mRNA from nonsense-mediated decay
Gene Wiki entry for A1CF (ACF)
Genomic Location for A1CF
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 10q11.23 Ensembl cytogenetic band: 10q11.23 HGNC cytogenetic band: 10q21.1 A1CF Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 10 GeneLoc Exon Structure
GeneLoc location for GC10M052237:
(about GC identifiers )
Start:
52,236,328 bp from pter
End:
52,315,441 bp from pter
Size:
79,114 bases
Orientation:
minus strand
RefSeq DNA sequence: NC_000010.9 NT_008583.16 Proteins for A1CF
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: A1CF_HUMAN, Q9NQ94 (See
protein sequence )Recommended Name: APOBEC1 complementation factor Size : 594 amino acids; 65202 Da
Subunit : Part of the apolipoprotein B mRNA editing complex with APOBEC1. Found in a complex withAPOBEC1 and CUGBP2. Interacts with KHSRP/KRSP which is not required for APOBEC1 activity but whichmay confer stability on the editing complex. Also interacts with the nuclear import proteinTNPO2/TRN2 which may be responsible for its transport into the nucleus
Subcellular location : Nucleus. Endoplasmic reticulum (By similarity). Cytoplasm. Note=Predominantlynuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outersurface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm.May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1
Sequence caution : Sequence=BAA91086.1; Type=Frameshift; Positions=148;
PDB structures from and Proteopedia : 2CPD (3D)
 
Secondary accessions : Q5SZQ0 Q9NQ93 Q9NQX8 Q9NQX9 Q9NXC9 Q9NZD3Alternative splicing : 6 isoforms : Q9NQ94-1 Q9NQ94-2 Q9NQ94-3 Q9NQ94-4 Q9NQ94-5 Q9NQ94-6
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (3 alternative transcripts):
NP_055391.2 NP_620310.1 NP_620311.1 ENSEMBL proteins: ENSP00000378867 ENSP00000378873 ENSP00000363107 ENSP00000378868 ENSP00000363105 ENSP00000363113 ENSP00000282641 ENSP00000363109 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 4 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for A1CF: Assays for A1CF:
Protein
Domains/ Families for A1CF(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q9NQ94 ProtoNet protein and cluster: Q9NQ94
1 Blocks protein family : IPB000504 RNA-binding region RNP-1 (RNA recognition motif) UniProtKB/Swiss-Prot: A1CF_HUMAN, Q9NQ94 Domain : The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additionalresidues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementingAPOBEC1 activity Similarity : Contains 3 RRM (RNA recognition motif) domains
Gene Function for A1CF
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
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SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): NM_014576 Applied Biosystems Silencer ® siRNAs for A1CF Sigma-Aldrich siRNA for A1CF Sigma-Aldrich shRNA for A1CF Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 3 ): NM_014576                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 3 ): NM_014576                                  untagged cDNA clones in CMV expression vector (see all 3 ): NM_014576  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_138932 UniProtKB/Swiss-Prot: A1CF_HUMAN, Q9NQ94 Function : Essential component of the apolipoprotein B mRNA editing enzyme complex which isresponsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop inAPOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit,APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects theedited APOB mRNA from nonsense-mediated decay
2 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for A1cf) :4 Gene Ontology (GO) molecular function terms (links to tree view) :
About this table
Pathways & Interactions for A1CF
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for A1CF (Your Favorite Gene powered by Ingenuity) 5/24 Interacting proteins for A1CF (ENSP00000363105 3 ) via UniProtKB, MINT, and/or STRING (see all 24
)About this table 2 Gene Ontology (GO) biological process terms (links to tree view) :
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Drugs & Compounds for A1CF (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for A1CF 3 Novoseek chemical compound relationships for A1CF gene
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Transcripts for A1CF(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
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siRNAs from Applied Biosystems ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): NM_014576 Sigma-Aldrich siRNA for A1CF Sigma-Aldrich shRNA for A1CF Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_014576 NM_138932 NM_138933
REFSEQ mRNAs for A1CF gene (3 alternative transcripts): NM_014576.2 NM_138932.1 NM_138933.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_014576 NM_138932 NM_138933
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3 ): NM_014576                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 3 ): NM_014576                                  untagged cDNA clones in CMV expression vector (see all 3 ): NM_014576  
Additional cDNA sequence: AF209192.1 AF271789.1 AF271790.1 AJ272078.1 AJ272079.1 AK000324.1 AK291982.1 AK303801.1 BC022263.1 BC054873.1 BC130519.1 BC144194.1 BC144196.1
9 DOTS entries : DT.92425892 DT.95374373 DT.100022638 DT.86841758 DT.100765861 DT.95374374 DT.99940798 DT.92425886 DT.95374372
24/110 AceView cDNA sequences (see all 110
):BM479119 AJ272078 AJ272079 CA942681 CA942922 T39997 NM_014576 BC054873 BG617808 T78557 AF271789 NM_138933 AI913746 CK825749 BF431668 CK825748 NM_138932 AW450191 AV684607 BQ269447 BV199907 AI672811 BU580671 AI760643
Unigene Cluster for A1CF: APOBEC1 complementation factor Hs.499643 [show with all ESTs ] Unigene Representative Sequence: AK303801 GeneLoc Exon Structure 5 Alternative Splicing Database (ASD) splice patterns (SP) for A1CF ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 SP1 :   -             -     -         -         SP2 :         -       -     -         -         SP3 :         -       -     -                 SP4 :                                   SP5 :   -       -                          
About this scheme ECgene alternative splicing isoforms for A1CF 8 Ensembl transcripts including schematic representations : ENST00000395488
ENST00000395495
ENST00000373995
ENST00000395489
ENST00000373993
ENST00000374001
ENST00000282641
ENST00000373997
Expression for A1CF
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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A1CF expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for A1CF 1 / 2
3 probe-sets matching A1CF gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: --SOURCE GeneReport for Unigene cluster: Hs.499643 UniProtKB/Swiss-Prot: A1CF_HUMAN, Q9NQ94 Tissue specificity : Widely expressed with highest levels in brain, liver, pancreas, colon andspleen
Orthologs for A1CF
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for A1CF gene from 5/7 species (see all 7
)
About this table Species with no ortholog for A1CF ENSEMBL Gene Tree for A1CF Paralogs for A1CF (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for A1CF gene RBM47 2 RBM46 2
SNPs/Variants for A1CF (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for A1CF (up to first 250kb)
Disorders & Mutations for A1CF
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
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and/or TGDB .)
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Medical News for A1CF (Possibly Related Articles in
Doctor's Guide )
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--
Publications for A1CF (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/27 PubMed articles for A1CF gene (see all 27
): A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling. (PubMed id 12896982) 1, 3, 4 Blanc V.... Davidson N.O. (2003) Molecular cloning of apobec-1 complementation factor, a novel RNA- binding protein involved in the editing of apolipoprotein B mRNA. (PubMed id 10669759) 1, 3, 4 Mehta A.... Driscoll D.M. (2000) Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing. (PubMed id 11815617) 1, 2, 3 Dance G.S....Smith H.C. (2002) Isolation, characterization and developmental regulation of the human apobec-1 complementation factor (ACF) gene. (PubMed id 11718896) 1, 3, 4 Henderson J.O.... Davidson N.O. (2001) Identification of domains in apobec-1 complementation factor required for RNA binding and apolipoprotein-B mRNA editing. (PubMed id 11871661) 1, 3, 4 Mehta A. and Driscoll D.M. (2002) The apolipoprotein B mRNA editing complex performs a multifunctional cycle and suppresses nonsense-mediated decay. (PubMed id 12881431) 1, 3, 4 Chester A.... Navaratnam N. (2003) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3, 4 Ota T....Sugano S. (2004) The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054) 3, 4 Deloukas P.... Rogers J. (2004) Novel role for RNA-binding protein CUGBP2 in mammalian RNA editing. CUGBP2 modulates C to U editing of apolipoprotein B mRNA by interacting with apobec-1 and ACF, the apobec-1 complementation factor. (PubMed id 11577082) 3, 4 Anant S.... Davidson N.O. (2001) Purification and molecular cloning of a novel essential component of the apolipoprotein B mRNA editing enzyme-complex. (PubMed id 10781591) 3, 4 Lellek H.... Greeve J. (2000)
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Genome Databases showing A1CF
(According to
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miRBase ,
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and/or
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Other Databases showing A1CF
(According to HUGE )
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Specialized Databases showing A1CF (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for A1CF (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for A1CF:
Recombinant Proteins (A1CF ) Antibodies (A1CF )
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