TUBB8 Gene
protein-coding GIFtS: 44
GCID: GC10M000093
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tubulin, beta 8 class VIII
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Aliases
for TUBB8 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Tubulin, Beta 8 Class VIII1 2 | | BA631M21.21 | | Class VIII Beta-Tubulin2 | | HSA10p15 Beta-Tubulin 4Q2 | | Tubulin Beta-8 Chain2 |
Export aliases for TUBB8 gene to outside databasesPrevious GC identifers: GC10M000082 GC10M000037 |
Summaries
for TUBB8 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: TBB8_HUMAN, Q3ZCM7Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site onthe beta chain and one at a non-exchangeable site on the alpha chain (By similarity)
|
Genomic Views
for TUBB8 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000010.10 NC_018921.1 NT_008705.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the TUBB8 gene promoter:
HOXA9B HOXA9 TBP SRF Tal-1 p53 SRF (504 AA) E47 ZID Meis-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for TUBB8
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TUBB8 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 10p15.3 Ensembl cytogenetic band: 10p15.3 HGNC cytogenetic band: 10p15.3TUBB8 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 10 GeneLoc Exon Structure GeneLoc location for GC10M000093: view genomic region
(about GC identifiers)
Start:
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92,828 bp from pter |
End:
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96,053 bp from pter |
Size:
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3,226 bases |
Orientation:
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minus strand |
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Proteins
for TUBB8 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: TBB8_HUMAN, Q3ZCM7 (See
protein sequence)Recommended Name: Tubulin beta-8 chain Size: 444 amino acids; 49776 Da
Subunit: Dimer of alpha and beta chains (By similarity)
Subcellular location: Cytoplasm, cytoskeleton (By similarity)
Sequence caution: Sequence=CAI16221.1; Type=Erroneous gene model prediction;
Secondary accessions: Q5SQX9 Q8WZ78Explore the universe of human proteins at neXtProt for TUBB8: NX_Q3ZCM7
Post-translational modifications:
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamateresidues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylateddue to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated intoaxonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and themitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylationlevels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear butthey regulate the assembly and dynamics of axonemal microtubules (Probable)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q3ZCM7
TUBB8 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_817124.1
ENSEMBL proteins: ENSP00000272035 ENSP00000418077 ENSP00000328808 ENSP00000311042 ENSP00000371071 ENSP00000417592 ENSP00000403895
Human Recombinant Protein Products:
Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table
TUBB8 for ontologies About GeneDecksing
TUBB8 Antibody Products:
Assay Products for TUBB8: |
Protein
Domains /
Families
for TUBB8 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TUBB8 for domains About GeneDecksing
5/8 InterPro domains/families (see all 8):Graphical View of Domain Structure for InterPro Entry Q3ZCM7ProtoNet protein and cluster: Q3ZCM7 1 Blocks protein family: IPB002453 Beta tubulin
UniProtKB/Swiss-Prot: TBB8_HUMAN, Q3ZCM7Similarity: Belongs to the tubulin family |
Function
for TUBB8 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: TBB8_HUMAN, Q3ZCM7Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site onthe beta chain and one at a non-exchangeable site on the alpha chain (By similarity)
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TUBB8 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TUBB8 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: TUBB8 (NM_177987) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TUBB8 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TUBB8 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table
TUBB8 for ontologies About GeneDecksing
|
Pathways & Interactions
for TUBB8 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/12 super-pathways (see all 12) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Pathogenic Escherichia coli infection | | | 2 | wtCFTR and deltaF508 traffic / Membrane expression (norm and CF) | | | 3 | Proteolysis Role of Parkin in the Ubiquitin-Proteasomal Pathway | | | 4 | Cell cycle_Spindle assembly and chromosome separation | | | 5 | Cytoskeleton remodeling_Reverse signaling by ephrin B | |
Pathway sources
See GeneCards unified pathways
Show all pathways
5/10  GeneGo (Thomson Reuters) Pathways for TUBB8 (see all 10)
2  BioSystems Pathways for TUBB8
3 
Kegg Pathways (Kegg details for TUBB8):
TUBB8 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TUBB8
STRING Interaction
Network Preview (showing 5 interactants - click image to see 10)
5/12 Interacting proteins for TUBB8 (Q3ZCM72, 3 ENSP000003288084) via UniProtKB, MINT, STRING, and/or I2D (see all 12)About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007018 | microtubule-based movement |
IEA | -- | | GO:0051258 | protein polymerization |
IEA | -- |
TUBB8 for ontologies About GeneDecksing
|
Drugs & Compounds
for TUBB8 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for TUBB8
1 HMDB Compound for TUBB8 About this table
Search CenterWatch for drugs/clinical trials and news about TUBB8 / TBB8
|
Transcripts
for TUBB8 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for TUBB8 gene (2 alternative transcripts): NM_001164154.1 NM_177987.2 Unigene Cluster for TUBB8: Tubulin, beta 8 class VIII Hs.532659 [show with all ESTs]Unigene Representative Sequence: NM_1779878 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000272035 ENST00000440680 ENST00000328974 ENST00000309812 ENST00000332708
ENST00000413237 ENST00000482075 ENST00000447903(uc001ifi.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TUBB8 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TUBB8 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: TUBB8 (NM_177987) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TUBB8 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TUBB8 |
Additional cDNA sequence: BC101270.2 BC101271.1 1 DOTS entry: DT.102840564 17 AceView cDNA sequences: CR626121 CR592383 CR592105 CR600137 CR612709 CR590375 CR603249 CR601851 CR603378 CR591556 CR599052 CR621858 CR606225 CR605516 CR592639 CR614889 NM_177987
GeneLoc Exon Structure
|
Expression
for TUBB8 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TUBB8 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See TUBB8 Protein Expression from SPIRE MOPED and PaxDB
SOURCE GeneReport for Unigene cluster: Hs.532659
SABiosciences Expression via Pathway-Focused PCR Array including TUBB8: | Gap Junctions in human mouse rat |
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TUBB8
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat TUBB8 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TUBB8 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TUBB8 |
Orthologs
for TUBB8 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for TUBB8 gene from 2/10 species (see all 10) About this table
ENSEMBL Gene Tree for TUBB8 (if available)
TreeFam Gene Tree for TUBB8 (if available) |
Paralogs
for TUBB8 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for TUBB8 gene
- TUBB2 TUBB4A2 TUBB4B2 TUBB2B2 TUBB32 ENSG000001732132 TUBB2A2 TUBB62
- TUBE12 TUBB12 ENSG000002589472
18/30 SIMAP similar genes for TUBB8 using alignment to 7 protein entries: TBB8_HUMAN (see all proteins)
(see all similar genes):DKFZp566F223 XTP3TPATP1 TUBB4Q TUBB2B TUBB2C TUBB4A
TUBB4B TUBB2A TUBB TUBB6 TUBB3 TUBB1
LOC402300 TUBA1 MGC16703 TUBA1A TUBA4A TUBG1
TUBB8 for paralogs About GeneDecksing
5/9 Pseudogenes.org Pseudogenes for TUBB8 (see all 9)
PGOHUM00000251097 PGOHUM00000256869 PGOHUM00000244573 PGOHUM00000245232 PGOHUM00000256887
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Genomic Variants
for TUBB8 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 10 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for TUBB8 (92828 - 96053 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for TUBB8: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TUBB8 |
|
Disorders
/ Diseases
for TUBB8 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TUBB8 for disorders About GeneDecksing
1  disease for TUBB8: About MalaCardsmalaria
Export disorders for TUBB8 gene to outside databases
|
Publications
for TUBB8 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TUBB8 gene, integrated from 9 sources (see all 18):
(articles sorted by number of sources associating them with TUBB8) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- A cascade of complex subtelomeric duplications during the evolution of the hominoid and Old World monkey genomes. (PubMed id 11731935)1, 2 van Geel M.... de Jong P.J. (2002)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Identification of SUMOylated proteins in neuroblastoma cells after treatment with hydrogen peroxide or ascorbate. (PubMed id 21110914)1 Grant M.M. (2010)
- Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. (PubMed id 19524510)2 Rogowski K....Janke C. (2009)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- HSP27 and HSP70 interact with CD10 in C4-2 prostate cancer cells. (PubMed id 17342744)1 Dall'Era M.A....Liu A.Y. (2007)
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External Searches for TUBB8 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing TUBB8 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TUBB8 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TUBB8 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for TUBB8 gene:
Search GeneIP for patents involving TUBB8
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for TUBB8 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
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 | |
 |
| | |  | OriGene Antibodies for TUBB8 | | OriGene shRNA RFP for TUBB8 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TUBB8 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TUBB8 | | | OriGene Protein Over-expression Lysate for TUBB8 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for TUBB8 | | OriGene 3'-UTR Clone for TUBB8 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TUBB8 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TUBB8 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for TUBB8 | | OriGene Custom Protein Services for TUBB8 | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat TUBB8 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TUBB8 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TUBB8 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TUBB8 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TUBB8 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TUBB8 |
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| | | | Search Tocris compounds for TUBB8 |
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 | | | | |  |  |  | | | | ThermoFisher Antibody for TUBB8 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TUBB8 |
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