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SQLE Gene

protein-coding   GIFtS: 59

GC08P126079
squalene epoxidase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 1.14.99.7 3
ERG1 3
FLJ30795 2
SE 3
Descriptions
squalene epoxidase 2, 3
squalene monooxygenase 2
External Ids
HGNC: 112791
Entrez Gene: 67132
UniProtKB: Q145343
Ensembl: ENSG000001045497
Search outside databases for aliases for SQLE gene

Previous GC identifers: GC08P125035 GC08P126015 GC08P125679 GC08P125968

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for SQLE:
Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be
one of the rate-limiting enzymes in this pathway. [provided by RefSeq]

UniProtKB/Swiss-Prot: ERG1_HUMAN, Q14534
Function: Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of
the rate-limiting enzymes in this pathway

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SQLE gene  

Entrez Gene cytogenetic band: 8q24.1   Ensembl cytogenetic band:  8q24.13   HGNC cytogenetic band: 8q24.1

SQLE Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P126079:     (about GC identifiers)

Start:
126,079,901 bp from pter
End:
126,103,707 bp from pter
Size:
23,807 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000008.9  NT_008046.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ERG1_HUMAN, Q14534 (See protein sequence)
Recommended Name: Squalene monooxygenase  
Size: 574 amino acids; 63939 Da
Cofactor: FAD
Subunit: May form a complex with squalene synthase
Subcellular location: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum
membrane; Multi-pass membrane protein
Secondary accessions: Q9UEK6

REFSEQ proteins: NP_003120.2  

ENSEMBL proteins: 
ENSP00000265896 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Squalene Epoxidase )
Human Recombinant Proteins from Abnova (SQLE)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

5 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005783 endoplasmic reticulum IEA--
GO:0005789 endoplasmic reticulum membrane IEA--
GO:0005792 microsome NAS--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for SQLE: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for SQLE
Browse R&D Systems for Antibodies
Antibodies from Abcam (Squalene Epoxidase ), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (SQLE)
Novus Biologicals Antibodies for SQLE

Assays for SQLE: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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3 InterPro domains/families:
 IPR006076 FAD-dep_OxRdtase
 IPR013698 Squalene_epoxidase
 IPR003042 Rng_hydrolase-like


   GeneDecks  SQLE for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q14534

ProtoNet protein and cluster: Q14534

1 Blocks protein family: IPB013698 Squalene epoxidase

UniProtKB/Swiss-Prot: ERG1_HUMAN, Q14534
Similarity: Belongs to the squalene monooxygenase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (SQLE)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003129

              Applied Biosystems Silencer® siRNAs for SQLE

              Sigma-Aldrich siRNA and siRNA Panels for SQLE  
                     Sigma-Aldrich shRNA for SQLE  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for SQLE
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003129
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003129
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_003129 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_003129

UniProtKB/Swiss-Prot: ERG1_HUMAN, Q14534
Function: Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of
the rate-limiting enzymes in this pathway
Catalytic activity: Squalene + AH(2) + O(2) = (S)-squalene-2,3-epoxide + A + H(2)O
Enzyme Number (IUBMB): EC 1.14.99.7 

Genatlas biochemistry entry for SQLE:
squalene epoxidase catalyzing the first oxygenation step in sterol biosynthesis

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004506 squalene monooxygenase activity NAS9286711
GO:0016491 oxidoreductase activity IEA--
GO:0050660 FAD binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for SQLE (Maps provided by GeneGo):
 Cholesterol metabolism I Cholesterol Biosynthesis

   GeneDecks  SQLE for the pathways selected above  
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  SQLE  (Your Favorite Gene powered by Ingenuity) 
 Biosynthesis of Steroids

   GeneDecks  SQLE for the pathways selected above  
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2 Kegg Pathways  (Kegg details for SQLE):
 hsa00100 Biosynthesis of steroids
 hsa00900 Terpenoid biosynthesis

   GeneDecks  SQLE for the pathways selected above  
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UniProtKB/Swiss-Prot: ERG1_HUMAN, Q14534
Pathway: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl-PP: step 2/3

 Gene Network CentralTM Interacting Genes and Proteins Network for  SQLE 


5/13 Interacting proteins for SQLE (ENSP000002658963) via UniProtKB, MINT, and/or STRING (see all 13 )

InteractantInteraction Details
GeneCardExternal ID(s)
FDFT1ENSP000002205843STRING (score=.972)
LSSENSP000003487623STRING (score=.97)
FDPSENSP000003573403STRING (score=.923)
MVDENSP000003010123STRING (score=.923)
IDI1ENSP000003707483STRING (score=.893)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016126 sterol biosynthetic process NAS9286711
GO:0055114 oxidation reduction IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for SQLE
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for SQLE
10/14 Novoseek chemical compound relationships for SQLE gene (see all 14 )
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
squalene 97.18 152 1964954 (7), 11181753 (6), 9324936 (5), 9300786 (4) (see all 71)
dimethyltelluride 89.80 1 11181753 (1)
ergosterol 84.08 16 18096502 (2), 12477372 (2), 16466954 (2), 1287164 (1) (see all 9)
lanosterol 82.12 5 16079485 (1), 7791529 (1), 15893902 (1)
sterol 74.67 10 8626488 (1), 7791529 (1), 1803242 (1), 9286711 (1) (see all 8)
tellurium 69.31 9 11181753 (5), 8090356 (1)
25-hydroxycholesterol 64.01 2 8626488 (1), 16098143 (1)
mevalonate 63.80 2 1964954 (1), 10082320 (1)
cholesterol 60.75 40 1649182 (3), 9300786 (2), 8347152 (2), 11181753 (2) (see all 26)
triacylglycerol 35.60 2 8504141 (2)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (SQLE)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003129

              Sigma-Aldrich siRNA and siRNA Panels for SQLE  
                     Sigma-Aldrich shRNA for SQLE  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_003129  

REFSEQ mRNAs for SQLE gene: 

NM_003129.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_003129  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003129
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003129
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_003129 

Additional cDNA sequence: 

AF098865.1 AK225085.1 AK295302.1 AK301792.1 AK313384.1 BC017033.1 BX647400.1 BX647605.1 
CR599266.1 D78129.1 D78130.1 

11 DOTS entries:

DT.97857247  DT.117824  DT.40299615  DT.95210936  DT.100757709  DT.92441125  DT.95210960  DT.75155650 
DT.92441126  DT.92473475  DT.95210930 

24/265 AceView cDNA sequences (see all 265 ):

AA341650 F05344 AI802289 AK055357 BP872774 AI341546 AL041945 AA580614 
AI810962 AI084342 BP368846 AI937469 BC017033 AL046067 BU631333 W80742 
BF154931 Z38352 AU136459 BI492283 CK300375 AI143944 AA132213 BI324843 

highest scoring ESTs for SQLE:

D78130 AA076231 AA101516 AA113270 AA114251 AA152331 AA158138 AA232318 AA286783 AA400196 

Unigene Cluster for SQLE:

Squalene epoxidase
Hs.71465  [show with all ESTs]
Unigene Representative Sequence: NM_003129


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000265896  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

SQLE expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for SQLE

1 / 2 / 3

8 probe-sets matching SQLE gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
35839_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D78130 1.00 1.00 1.00 1

213577_at2, 3 U133-A 1 1.00 1.00 -- -- AA639705 0.40 1.00 0.76 1

213562_s_at2, 3 U133-A 1 1.00 1.00 -- -- BF979497 0.40 1.00 0.76 1

209218_at2, 3 U133-A 1 1.00 1.00 -- -- AF098865 0.80 1.00 0.91 1

1557352_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

213577_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

213562_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209218_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  SQLE for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CAAATAAAAT

SOURCE GeneReport for Unigene cluster: Hs.71465

Expression variation in blood from EXPOLDB for SQLE

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for SQLE gene from 5/19 species (see all 19 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
SQLE1   -- squalene epoxidase 88.6(n)
87.61(a)
608021  XM_856851.1  XP_861944.1 
chimpanzee
(Pan troglodytes)
SQLE1   -- squalene epoxidase 99.48(n)
99.3(a)
464383  XM_519950.2  XP_519950.2 
cow
(Bos taurus)
SQLE1   -- squalene epoxidase 89.04(n)
87.94(a)
526535  XM_604908.3  XP_604908.3 
rat
(Rattus norvegicus)
Sqle1   -- squalene epoxidase 83.01(n)
83.94(a)
29230  NM_017136.1  NP_058832.1 
mouse
(Mus musculus)
Sqle1, 5 155
squalene epoxidase1, 5 82.98(n)1
84.44(a)1
207751  NM_009270.31  NP_033296.11 
 AI3237925  AK0411985  (see all 32)
About this table        Species with no ortholog for SQLE

ENSEMBL Gene Tree for SQLE
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/180 NCBI SNPs in SQLE are shown (see all 180 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 94)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 8 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs69946861,2
A,C,F,H126080233(+) CGGCGT/CTGGCG 1 -- ut514Minor allele frequency- C:0.07EU EA WA 408
rs38532551,2
A,C,F,H126079012(-) AGTCTC/TGGAGT 1 -- ng514Minor allele frequency- T:0.06EU EA WA 418
rs126761281,2
A,C,F,H126104093(+) AACAAG/ATAAGC 1 -- ng3115Minor allele frequency- A:0.35NA EU EA WA 1336
rs99401,2
C,F,H126103592(+) CATTTA/GAAAGA 1 -- ut31 ese38Minor allele frequency- G:0.05MN EU EA WA NA 742
rs18025261,2
C,F,H126103557(+) TTGCAA/GTTTAA 1 -- ut314Minor allele frequency- G:0.04EU EA WA 418
rs38176881,2
F,H126103765(-) TCTTTT/CTCATT 1 -- ng314Minor allele frequency- C:0.02EU EA WA 414
rs69950071,2
F,H126080408(+) CCCATT/GCCCTT 1 -- ut513Minor allele frequency- G:0.02EU EA 250
rs48709341,2
H126103643(+) CTACAG/CTTTTT 1 -- ut31 ese34Minor allele frequency- C:0.00EU EA WA 414
rs10488581,2
H126084723(+) TTTGGC/TAGCTG 1 A/V mis1 ese35Minor allele frequency- T:0.00MN EU EA WA 590
--
rs70124881,2
A126080122(+) GGCGGC/GTCTGG 1 -- ut510--------
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HapMap Linkage Disequilibrium images for SQLE (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 602019

3 Novoseek disease relationships for SQLE gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
demyelination 31.84 1 11181753 (1)
infection fungal 31.54 2 17531123 (1), 12956204 (1)
hypercholesterolemia 29.64 1 1649182 (1)
About this table

Human Genome Epidemiology Navigator: SQLE (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/82 PubMed articles for SQLE gene (see all 82 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 6713 HGNC: 11279 AceView: SQLE Ensembl:ENSG00000104549 euGenes: HUgn6713
ECgene: SQLE H-InvDB: SQLE
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for SQLE:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for SQLE-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for SQLE Antibodies for SQLE
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for SQLE Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(Squalene Epoxidase )
 Antibodies (Squalene Epoxidase )
 Search OriGene for SQLE
 Search Tocris compounds for SQLE




 Drugs for SQLE
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for SQLE

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