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Aliases for PFKFB4 Gene

Aliases for PFKFB4 Gene

  • 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 4 2 3
  • 6PF-2-K/Fru-2,6-P2ase Testis-Type Isozyme 3 4
  • 6PF-2-K/Fru-2,6-P2ase 4 3 4
  • PFK/FBPase 4 3 4
  • Bifunctional Enzyme With Kinase And Biphosphatase Activities 3
  • 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 4 3
  • 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase-4 3

External Ids for PFKFB4 Gene

Previous GeneCards Identifiers for PFKFB4 Gene

  • GC03M048349
  • GC03M047841
  • GC03M048516
  • GC03M048530
  • GC03M048555

Summaries for PFKFB4 Gene

GeneCards Summary for PFKFB4 Gene

PFKFB4 (6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 4) is a Protein Coding gene. Among its related pathways are Akt Signaling and Metabolism. GO annotations related to this gene include fructose-2,6-bisphosphate 2-phosphatase activity and 6-phosphofructo-2-kinase activity. An important paralog of this gene is PFKFB3.

UniProtKB/Swiss-Prot for PFKFB4 Gene

  • Synthesis and degradation of fructose 2,6-bisphosphate

Gene Wiki entry for PFKFB4 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PFKFB4 Gene

Genomics for PFKFB4 Gene

Regulatory Elements for PFKFB4 Gene

Transcription factor binding sites by QIAGEN in the PFKFB4 gene promoter:

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for PFKFB4 Gene

48,517,684 bp from pter
48,562,015 bp from pter
44,332 bases
Minus strand

Genomic View for PFKFB4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PFKFB4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PFKFB4 Gene

Proteins for PFKFB4 Gene

  • Protein details for PFKFB4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
    Protein Accession:
    Secondary Accessions:
    • Q5S3G5
    • Q5XLC2
    • Q64EX5

    Protein attributes for PFKFB4 Gene

    469 amino acids
    Molecular mass:
    54040 Da
    Quaternary structure:
    • Homodimer.

    Alternative splice isoforms for PFKFB4 Gene


neXtProt entry for PFKFB4 Gene

Proteomics data for PFKFB4 Gene at MOPED

Post-translational modifications for PFKFB4 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for PFKFB4 Gene

Antibody Products

No data available for DME Specific Peptides for PFKFB4 Gene

Domains for PFKFB4 Gene

Graphical View of Domain Structure for InterPro Entry



F264_HUMAN :
  • Q16877
  • In the C-terminal section; belongs to the phosphoglycerate mutase family.
genes like me logo Genes that share domains with PFKFB4: view

No data available for Gene Families for PFKFB4 Gene

Function for PFKFB4 Gene

Molecular function for PFKFB4 Gene

GENATLAS Biochemistry:
6-phosphofructo-2-kinase-fructose-2,6-biphosphatase bifunctional enzyme responsible for the synthesis and breakdown of fructose 2,6 biphosphate,key metabolite in the regulation of glycolysis,expressed in testis
UniProtKB/Swiss-Prot CatalyticActivity:
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
UniProtKB/Swiss-Prot EnzymeRegulation:
The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme.
UniProtKB/Swiss-Prot Function:
Synthesis and degradation of fructose 2,6-bisphosphate

Gene Ontology (GO) - Molecular Function for PFKFB4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003873 6-phosphofructo-2-kinase activity NAS 10095107
GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity IEA --
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with PFKFB4: view

Phenotypes for PFKFB4 Gene

GenomeRNAi human phenotypes for PFKFB4:
genes like me logo Genes that share phenotypes with PFKFB4: view

Animal Model Products

miRNA for PFKFB4 Gene

miRTarBase miRNAs that target PFKFB4

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PFKFB4

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for PFKFB4 Gene

Localization for PFKFB4 Gene

Subcellular locations from

Jensen Localization Image for PFKFB4 Gene COMPARTMENTS Subcellular localization image for PFKFB4 gene
Compartment Confidence
cytosol 4
mitochondrion 3
nucleus 1

Gene Ontology (GO) - Cellular Components for PFKFB4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with PFKFB4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for PFKFB4 Gene

Pathways for PFKFB4 Gene

genes like me logo Genes that share pathways with PFKFB4: view

Pathways by source for PFKFB4 Gene

2 Qiagen pathways for PFKFB4 Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for PFKFB4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process TAS --
GO:0006000 fructose metabolic process IEA --
GO:0006003 fructose 2,6-bisphosphate metabolic process IEA --
GO:0006006 glucose metabolic process TAS --
GO:0006096 glycolytic process --
genes like me logo Genes that share ontologies with PFKFB4: view

Compounds for PFKFB4 Gene

(7) HMDB Compounds for PFKFB4 Gene

Compound Synonyms Cas Number PubMed IDs
2-Phosphoglyceric acid
  • 2-(dihydrogen phosphate)Glycerate
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
  • adenosindiphosphorsaeure
Beta-D-Fructose 6-phosphate
  • beta-D-Fructose 6-phosphate
Not Available
D-Fructose 2,6-bisphosphate
  • D-Fructose 2,6-bisphosphate
Not Available

(2) Drugbank Compounds for PFKFB4 Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
Phosphoaminophosphonic Acid-Adenylate Ester

(3) Novoseek inferred chemical compound relationships for PFKFB4 Gene

Compound -log(P) Hits PubMed IDs
cobalt chloride 75.4 1
deferoxamine 56.5 1
vegf 51 4
genes like me logo Genes that share compounds with PFKFB4: view

Transcripts for PFKFB4 Gene

Unigene Clusters for PFKFB4 Gene

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PFKFB4

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PFKFB4 Gene

ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b
SP1: - - -
SP2: - - -
SP3: - - - - -
SP4: - - - -
SP5: - - - - -
SP6: - - - - -
SP7: - - - -
SP8: - - -
SP9: - - - - - -

Relevant External Links for PFKFB4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for PFKFB4 Gene

mRNA expression in normal human tissues for PFKFB4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PFKFB4 Gene

This gene is overexpressed in Whole Blood (18.1).

Protein differential expression in normal tissues for PFKFB4 Gene

This gene is overexpressed in Testis (37.3) and Ovary (23.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for PFKFB4 Gene

SOURCE GeneReport for Unigene cluster for PFKFB4 Gene Hs.476217

genes like me logo Genes that share expressions with PFKFB4: view

Expression partners for PFKFB4 Gene

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for PFKFB4 Gene

Orthologs for PFKFB4 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for PFKFB4 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia PFKFB4 35
  • 92.11 (n)
  • 98.51 (a)
  • 99 (a)
(Canis familiaris)
Mammalia PFKFB4 35
  • 92.4 (n)
  • 98.72 (a)
  • 83 (a)
(Mus musculus)
Mammalia Pfkfb4 35
  • 89.2 (n)
  • 97.44 (a)
Pfkfb4 16
Pfkfb4 36
  • 97 (a)
(Pan troglodytes)
Mammalia PFKFB4 35
  • 99.72 (n)
  • 100 (a)
  • 100 (a)
(Rattus norvegicus)
Mammalia Pfkfb4 35
  • 89.05 (n)
  • 97.23 (a)
(Monodelphis domestica)
Mammalia PFKFB4 36
  • 89 (a)
(Ornithorhynchus anatinus)
Mammalia PFKFB4 36
  • 96 (a)
(Gallus gallus)
Aves LOC100859653 35
  • 77.35 (n)
  • 92.22 (a)
  • 90 (a)
(Anolis carolinensis)
Reptilia PFKFB4 36
  • 88 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia pfkfb4 35
  • 73.89 (n)
  • 83.26 (a)
(Danio rerio)
Actinopterygii pfkfb4 35
pfkfb4l 35
  • 74.79 (n)
  • 83.33 (a)
pfkfb4 36
  • 79 (a)
pfkfb4l 36
  • 77 (a)
fruit fly
(Drosophila melanogaster)
Insecta Pfrx 36
  • 36 (a)
(Caenorhabditis elegans)
Secernentea K02B2.1 37
  • 51 (a)
Y110A7A.6a 37
  • 55 (a)
Y110A7A.6b 37
  • 56 (a)
K02B2.1 36
  • 48 (a)
Y110A7A.6 36
  • 53 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes FBP26 36
  • 46 (a)
-- 38
thale cress
(Arabidopsis thaliana)
eudicotyledons F2KP 35
  • 52.36 (n)
  • 48.27 (a)
(Oryza sativa)
Liliopsida Os05g0164100 35
  • 53.51 (n)
  • 49.07 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 63 (a)
Species with no ortholog for PFKFB4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PFKFB4 Gene

Gene Tree for PFKFB4 (if available)
Gene Tree for PFKFB4 (if available)

Paralogs for PFKFB4 Gene

Paralogs for PFKFB4 Gene

genes like me logo Genes that share paralogs with PFKFB4: view

Variants for PFKFB4 Gene

Sequence variations from dbSNP and Humsavar for PFKFB4 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs751872 -- 48,552,822(+) GTGGC(A/T)TTTCC intron-variant
rs766363 -- 48,523,650(+) GACAG(G/T)GCCTA intron-variant
rs1128386 -- 48,517,917(-) GGGTA(A/G)AGGGA utr-variant-3-prime
rs2006871 -- 48,549,264(+) CGACT(C/T)ACCCA intron-variant
rs2098101 -- 48,532,888(+) atgaa(C/G)cttga intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PFKFB4 Gene

Variant ID Type Subtype PubMed ID
nsv834684 CNV Gain 17160897
nsv523981 CNV Loss 19592680
nsv834685 CNV Loss 17160897
nsv876762 CNV Loss 21882294

Relevant External Links for PFKFB4 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PFKFB4 Gene

Disorders for PFKFB4 Gene

(1) Novoseek inferred disease relationships for PFKFB4 Gene

Disease -log(P) Hits PubMed IDs
cancer 25.6 10
genes like me logo Genes that share disorders with PFKFB4: view

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Genatlas and External Links for PFKFB4 Gene

Publications for PFKFB4 Gene

  1. Cloning, expression and chromosomal localization of a human testis 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene. (PMID: 10095107) Manzano A. … Bartrons R. (Gene 1999) 2 3 4 23
  2. Hypoxia induces transcription of 6-phosphofructo-2-kinase/fructose- 2,6-biphosphatase-4 gene via hypoxia-inducible factor-1alpha activation. (PMID: 15474002) Minchenko O. … Esumi H. (FEBS Lett. 2004) 3 4 23
  3. Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region. (PMID: 15642344) Gomez M. … Bartrons R. (FEBS Lett. 2005) 3 4 23
  4. Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human placenta. (PMID: 8830046) Sakai A. … Sakakibara R. (J. Biochem. 1996) 2 3 4
  5. Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and pancreatic cancer cell lines and expression of PFKFB genes in gastric cancers. (PMID: 17143338) Bobarykina A.Y. … Minchenko O.H. (Acta Biochim. Pol. 2006) 3 23

Products for PFKFB4 Gene

Sources for PFKFB4 Gene

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