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HTR1F Gene

protein-coding   GIFtS: 60

GC03P088122
5-hydroxytryptamine (serotonin) receptor 1F
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
5-HT-1F 3
5-HT1F 1, 2
5HT6 2
HTR1EL 1, 2, 3, 5
MR77 2, 5
Descriptions
5-hydroxytryptamine (serotonin) receptor 1F 2
5-hydroxytryptamine receptor 1F 2
Serotonin receptor 1F 3
External Ids
HGNC: 52921
Entrez Gene: 33552
UniProtKB: P309393
Ensembl: ENSG000001790977
Search outside databases for aliases for HTR1F gene

Previous GC identifers: GC03P088567 GC03P090821 GC03P087919 GC03P087960

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: 5HT1F_HUMAN, P30939
Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a
biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of
this receptor is mediated by G proteins that inhibit adenylate cyclase activity

Gene Wiki entry for HTR1F (5-HT1F_receptor)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the HTR1F gene  

Entrez Gene cytogenetic band: 3p12   Ensembl cytogenetic band:  3p11.2   HGNC cytogenetic band: 3p12

HTR1F Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P088122:     (about GC identifiers)

Start:
88,114,416 bp from pter
End:
88,125,609 bp from pter
Size:
11,194 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_022459.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: 5HT1F_HUMAN, P30939 (See protein sequence)
Recommended Name: 5-hydroxytryptamine receptor 1F  
Size: 366 amino acids; 41709 Da
Subcellular location: Cell membrane; Multi-pass membrane protein

REFSEQ proteins: NP_000857.1  

ENSEMBL proteins: 
ENSP00000322924 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (5HT1F Receptor)
Human Recombinant Proteins from Abnova (HTR1F)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane IEA--
GO:0005887 integral to plasma membrane TAS8380639
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Antibodies for HTR1F: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for HTR1F
Browse R&D Systems for Antibodies
Antibodies from Abcam (5HT1F Receptor), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (HTR1F)
Novus Biologicals Antibodies for HTR1F

Assays for HTR1F: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR017452 GPCR_Rhodpsn_supfam
 IPR000450 5HT1F_rcpt
 IPR002231 5HT_rcpt
 IPR000276 7TM_GPCR_Rhodpsn


   GeneDecks  HTR1F for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P30939

ProtoNet protein and cluster: P30939

2 Blocks protein families:
IPB000450 5-hydroxytryptamine 1F receptor signature
IPB002231 5-hydroxytryptamine receptor signature


UniProtKB/Swiss-Prot: 5HT1F_HUMAN, P30939
Similarity: Belongs to the G-protein coupled receptor 1 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HTR1F)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (HTR1F)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_000866

              Applied Biosystems Silencer® siRNAs for HTR1F

              Sigma-Aldrich siRNA and siRNA Panels for HTR1F  
                     Sigma-Aldrich shRNA Panels and shRNA for HTR1F  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for HTR1F
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_000866
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_000866
                                 untagged cDNA clone in CMV expression vector: NM_000866 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_000866

UniProtKB/Swiss-Prot: 5HT1F_HUMAN, P30939
Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a
biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of
this receptor is mediated by G proteins that inhibit adenylate cyclase activity

Genatlas biochemistry entry for HTR1F:
5-hydroxytryptamine (serotonin) receptor,subtype 1F,G protein coupled receptor superfamily

3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Htr1f):

behavior/neurologicalnervous systemtouch/vibrissae

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004872 receptor activity IEA--
GO:0004930 G-protein coupled receptor activity IEA--
GO:0004993 serotonin receptor activity TAS8380639
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  HTR1F  (Your Favorite Gene powered by Ingenuity) 
 G-Protein Coupled Receptor Signaling
 cAMP-mediated Signaling

   GeneDecks  HTR1F for the pathways selected above  
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1 Kegg Pathway  (Kegg details for HTR1F):
 hsa04080 Neuroactive ligand-receptor interaction

   GeneDecks  HTR1F for the pathways selected above  
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5/6 Interacting proteins for HTR1F (ENSP000003229243) via UniProtKB, MINT, and/or STRING (see all 6 )
InteractantInteraction Details
GeneCardExternal ID(s)
HTR5AENSP000002879073STRING (score=.909)
HTR1AENSP000003162443STRING (score=.907)
HTR1DENSP000003637483STRING (score=.905)
HTR1EENSP000003585973STRING (score=.905)
HTR3AENSP000003477543STRING (score=.865)
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3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007165 signal transduction IEA--
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger TAS8380639
GO:0007268 synaptic transmission TAS8380639
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for HTR1F
Sigma-Aldrich Small Molecules for HTR1F:
Metabolite Antagonist Agonist Allosteric Modulator Blocker Precursor
Inhibitor Ligand

Compounds for HTR1F available from Tocris Bioscience
CompoundAction CAS number
LY 334370 hydrochlorideSelective 5-HT1F agonist[182563-08-2]
LY 344864 hydrochloridePotent, selective 5-HT1F agonist[186544-26-3]
BRL 54443Potent 5-ht1E/F agonist
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HTR1F)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (HTR1F)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_000866

              Sigma-Aldrich siRNA and siRNA Panels for HTR1F  
                     Sigma-Aldrich shRNA Panels and shRNA for HTR1F  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_000866  

REFSEQ mRNAs for HTR1F gene: 

NM_000866.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_000866  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_000866
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_000866
                                 untagged cDNA clone in CMV expression vector: NM_000866 

Additional cDNA sequence: 

AF498981.1 BC069125.1 BC096838.1 CR606112.1 

2 DOTS entries:

DT.92061708  DT.100747290 

10 AceView cDNA sequences:

CR606112 NM_000866 AF498981 BC069125 CB565886 BM312885 CA942616 CA942859 
BM313179 AV758122 

highest scoring ESTs for HTR1F:

AF498981 BC069125 CB565886 BC096838 BM313179 BX327154 CA942859 CR606112 NM_000866 BX329042 

Unigene Cluster for HTR1F:

5-hydroxytryptamine (serotonin) receptor 1F
Hs.248136  [show with all ESTs]
Unigene Representative Sequence: NM_000866


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000319595  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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HTR1F expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for HTR1F

1 / 2 / 3

2 probe-sets matching HTR1F gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

221458_at2, 3 U133-A 1 1.00 1.00 -- -- NM_000866 0.60 1.00 0.82 1

221458_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.248136
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for HTR1F gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
HTR1F1   -- 5-hydroxytryptamine (serotonin) receptor 1F 99.73(n)
100(a)
470863  XM_526246.2  XP_526246.2 
cow
(Bos taurus)
HTR1F1   -- 5-hydroxytryptamine (serotonin) receptor 1F 91.35(n)
94.81(a)
407196  XM_001250472.1  XP_001250473.1 
rat
(Rattus norvegicus)
Htr1f1   -- 5-hydroxytryptamine (serotonin) receptor 1F 87.21(n)
92.6(a)
60448  NM_021857.1  NP_068629.1 
mouse
(Mus musculus)
Htr1f1, 5 16 (38.80 cM)5
5-hydroxytryptamine (serotonin) receptor 1F1, 5 87.95(n)1
94.52(a)1
155571  NM_008310.21  NP_032336.11 
 AK0453645  BC1205075  (see all 7)
chicken
(Gallus gallus)
HTR1F1   -- 5-hydroxytryptamine (serotonin) receptor 1F 79.06(n)
83.2(a)
427963  XM_425535.1  XP_425535.1 
About this table        Species with no ortholog for HTR1F

ENSEMBL Gene Tree for HTR1F
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
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Paralogs for HTR1F gene
HTR1D2  HTR1A2  HTR1E2  HTR1B2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/83 NCBI SNPs in HTR1F are shown (see all 83 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 15)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 3 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs20162241,2
A,C,F,H88124340(-) TAATAT/CATTAA 1 -- ut31 ese34Minor allele frequency- C:0.43EU EA WA 416
rs48589271,2
A,C,F,H88113379(+) AATTAG/ATTGAA 1 -- ng51 tfbs34Minor allele frequency- A:0.06EU EA WA 418
rs67700131,2
A,C,F88114388(+) aggctC/Acttag 1 -- ng512Minor allele frequency- A:0.38NA 182
--
rs48589251,2
A,C88113032(+) GGGttC/Tttttg 1 -- ng51 tfbs30--------
--
rs48589691,2
A,C88113186(+) cacacC/Tgccat 1 -- ng510--------
--
rs67782111,2
A,C88113976(+) cagacA/Gggtct 1 -- ng510--------
--
rs48589261,2
A,C88113235(+) tcaccA/Gtgttg 1 -- ng510--------
--
rs67812261,2
A,C88114526(+) ccctaA/Gtgaag 1 -- ut510--------
--
rs93100601,2
C88123970(+) ATATAC/TACAAT 1 -- ut31 ese30--------
--
rs20822261,2
H88112416(+) TCCCTG/AGCTTG 1 -- ng511Minor allele frequency- A:0.00WA 120
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HapMap Linkage Disequilibrium images for HTR1F (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 182134

1 Novoseek disease relationship for HTR1F gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
migraine 55.56 3 9949863 (2), 9225282 (1)
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Genetic Association Database: HTR1F
Human Genome Epidemiology Navigator: HTR1F (4 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/30 PubMed articles for HTR1F gene (see all 30 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3355 HGNC: 5292 AceView: HTR1F Ensembl:ENSG00000179097 euGenes: HUgn3355
ECgene: HTR1F H-InvDB: HTR1F
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for HTR1F:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Millipore Custom Antibody & Bulk Services
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 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for HTR1F Antibodies for HTR1F
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for HTR1F Small molecules for HTR1F
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
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 Search OriGene for HTR1F
 Tocris compounds for HTR1F




 Drugs for HTR1F
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for HTR1F

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